Journal: Nature neuroscience

Article Title: The exon junction complex component Magoh controls brain size by regulating neural stem cell division

doi: 10.1038/nn.2527

Figure Lengend Snippet: a,b , Graphs representing Lis1 gene expression measured by quantitative PCR ( a ) and LIS1 protein expression measured from Western blot analyses ( b ). b , Representative cropped Western blots of cortical lysates from indicated ages and genotypes, probed with antibodies against LIS1 (46 kDa) and α-Tubulin (55 kDa) as a loading control. Below the lanes are densitometry values normalized for loading, with control normalized to 1.0. The full-length blots are presented in . c , Confocal micrographs of coronal sections from E16.5 brains in utero co-electroporated at E13.5 with pCAG-GFP (green) and the following: Luciferase shRNA + vector, Magoh shRNA + vector, Magoh shRNA + Lis1 Luciferase shRNA + Lis1 . Note that in Magoh shRNA + vector brains there are fewer GFP+ cells in the SVZ/VZ layers. d , Graph depicting the percentage of GFP+ cells in lower SVZ/VZ and upper CP layers of the brain for indicated in utero electroporations. e , Confocal micrographs of brains from indicated in utero electroporations showing GFP (green) and stained for Tbr2 (red) and DAPI (blue). The inset shows a higher magnification view of the boxed region, depicting co-localization (yellow) of Tbr2 (red) and GFP (green). f , Graph depicting the percentage of GFP+ cells that express Tbr2 for indicated in utero electroporations. Graphs in ( d,f ) show averages of all sections. *, P <0.05, **, P <0.005; Error bars, s.d.; Scale bars, 100 µm.

Article Snippet: Lis1 was subcloned into pCAGGS vector from a pcDNA3- Lis1 construct (Addgene).

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Control, In Utero, Luciferase, shRNA, Plasmid Preparation, Staining

Journal: NPJ Biofilms and Microbiomes

Article Title: TSP50 deficiency in neural stem cells aggravates colitis in mice by altering intestinal microbiome

doi: 10.1038/s41522-025-00737-3

Figure Lengend Snippet: a Scheme of FMT and AKK replantation protocol. b Body weight loss after DSS treatment ( n = 6). c , d The DAI scores and survival rates after DSS treatment ( n = 6). e Colon lengths and quantification after DSS treatment ( n = 6). f Representative HE staining and histopathological scores of colon tissues after DSS treatment ( n = 6). Scale bar: 50 μm. g ELISA analysis of serum TNF-α levels after DSS treatment ( n = 4). h ELISA analysis of serum IL-6 levels after DSS treatment ( TSP50 fl/fl , n = 3; TSP50 fl/fl Nestin Cre , n = 3; FMT- TSP50 fl/fl Nestin Cre , n = 4; AKK- TSP50 fl/fl Nestin Cre , n = 4). Values were expressed as means ± SEM. Two‑way ANOVA was used to analyze ( b , c ), one‑way ANOVA was used to analyze ( e , f , g , h ). ns (no significance), * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The sections were incubated with primary antibody: anti-MUC2 (1:1000; ab272692, Abcam, Cambridge, United Kingdom), anti- TSP50 (1:500; 12574-1-AP, Proteintech, Wuhan, China), anti- AChE (1:20; ab2803, Abcam), anti- NESTIN (1:200; 19483-1-AP, Proteintech) and corresponding secondary antibody.

Techniques: Staining, Enzyme-linked Immunosorbent Assay

Journal: NPJ Biofilms and Microbiomes

Article Title: TSP50 deficiency in neural stem cells aggravates colitis in mice by altering intestinal microbiome

doi: 10.1038/s41522-025-00737-3

Figure Lengend Snippet: a ELISA analysis of ACh levels in the colon after ACh supplementation ( TSP50 fl/fl , n = 6; TSP50 fl/fl Nestin Cre , n = 6; ACh- TSP50 fl/fl , n = 8; ACh- TSP50 fl/fl Nestin Cre , n = 8). b PCoA analysis of fecal microbiota using weighted UniFrac distances of beta diversity after ACh supplementation. c Histogram of phylum-level species composition after ACh supplementation. d Histogram of genus-level species composition after ACh supplementation. e Histogram of species difference comparison for the top 10 abundances at phylum-level ( n = 6). f Histogram of species difference comparison for the top 10 abundances at genus-level ( n = 6). g Heat map of the relative abundance of the dominant genus. h LEfSe analysis of differential microbiota in TSP50 fl/fl mice before and after ACh treatment. i LEfSe analysis of differential microbiota in TSP50 fl/fl Nestin Cre mice before and after ACh treatment. j GC-MS analysis of fecal SCFAs after ACh supplementation ( n = 5). k ELISA analysis of ACh levels in the colon after ABX treatment ( n = 6). l ELISA analysis of ACh levels in the colon after co-housing treatment ( n = 6). m ELISA analysis of ACh levels in the colon after FMT and AKK replantation ( n = 6). Values were expressed as means ± SEM. One‑way ANOVA was used to analyze ( a , j , k , l , m ), Kruskal-Wallis H test was used to analyze ( e , f ). ns (no significance), * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The sections were incubated with primary antibody: anti-MUC2 (1:1000; ab272692, Abcam, Cambridge, United Kingdom), anti- TSP50 (1:500; 12574-1-AP, Proteintech, Wuhan, China), anti- AChE (1:20; ab2803, Abcam), anti- NESTIN (1:200; 19483-1-AP, Proteintech) and corresponding secondary antibody.

Techniques: Enzyme-linked Immunosorbent Assay, Comparison, Gas Chromatography-Mass Spectrometry

Journal: NPJ Biofilms and Microbiomes

Article Title: TSP50 deficiency in neural stem cells aggravates colitis in mice by altering intestinal microbiome

doi: 10.1038/s41522-025-00737-3

Figure Lengend Snippet: a WB analysis and quantification of AChE protein levels in mouse brain and colon ( n = 3). b Co-IP analysis of proteins interacting with TSP50 in mouse brain and colon. c GST pull-down of AChE by GST-TSP50 using proteins purified in B21 bacteria, followed by WB analysis. d WB analysis and quantification of AChE protein levels in control and TSP50-overexpressing 293T cells after CHX treatment ( n = 4). e WB analysis and quantification of AChE protein levels after incubation with TSP50 protein at 20 mM Tris-HCL (pH7.6) for 1 h at 37 °C ( n = 4). (Ctr: No incubation; 1 h: Incubation for 1 h) f WB analysis and quantification of TSP50 and AChE protein levels in 293T cells with normal and overexpression TSP50 and different TSP50 mutants ( n = 3). g Representative IHC staining of TSP50 and AChE protein expression in CD patient tissues and quantification ( n = 19). Scale bar: 50 μm. h Pearson correlation analysis was performed between the relative levels of TSP50 and AChE protein in samples of CD patients. Values were expressed as means ± SEM. One‑way ANOVA was used to analyze ( a , d , f , g ), unpaired two-tailed Student’s t test was used to analyze ( e ). ns (no significance), * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The sections were incubated with primary antibody: anti-MUC2 (1:1000; ab272692, Abcam, Cambridge, United Kingdom), anti- TSP50 (1:500; 12574-1-AP, Proteintech, Wuhan, China), anti- AChE (1:20; ab2803, Abcam), anti- NESTIN (1:200; 19483-1-AP, Proteintech) and corresponding secondary antibody.

Techniques: Co-Immunoprecipitation Assay, Purification, Bacteria, Control, Incubation, Over Expression, Immunohistochemistry, Expressing, Two Tailed Test

Journal: NPJ Biofilms and Microbiomes

Article Title: TSP50 deficiency in neural stem cells aggravates colitis in mice by altering intestinal microbiome

doi: 10.1038/s41522-025-00737-3

Figure Lengend Snippet: a Scheme of NMS treatment protocol. b Body weight loss after DSS treatment ( TSP50 fl/fl , n = 8; TSP50 fl/fl Nestin Cre , n = 8; NMS- TSP50 fl/fl , n = 6; NMS- TSP50 fl/fl Nestin Cre , n = 6). c , d The DAI scores and survival rates after DSS treatment ( TSP50 fl/fl , n = 8; TSP50 fl/fl Nestin Cre , n = 8; NMS- TSP50 fl/fl , n = 6; NMS- TSP50 fl/fl Nestin Cre , n = 6). e Colon lengths and quantification after DSS treatment ( TSP50 fl/fl , n = 8; TSP50 fl/fl Nestin Cre , n = 8; NMS- TSP50 fl/fl , n = 6; NMS- TSP50 fl/fl Nestin Cre , n = 6). (T fl/fl : TSP50 fl/fl ; T fl/fl N Cre : TSP50 fl/fl Nestin Cre ; NMS-T fl/fl : NMS- TSP50 fl/fl ; NMS-T fl/fl N Cre : NMS- TSP50 fl/fl Nestin Cre ). f Representative HE staining and histopathological scores of colon tissues after DSS treatment ( n = 6). Scale bar: 50 μm. g ELISA analysis of serum TNF-α levels after DSS treatment ( TSP50 fl/fl , n = 6; TSP50 fl/fl Nestin Cre , n = 6; NMS- TSP50 fl/fl , n = 5; NMS- TSP50 fl/fl Nestin Cre , n = 5). h ELISA analysis of serum IL-6 levels after DSS treatment ( TSP50 fl/fl , n = 8; TSP50 fl/fl Nestin Cre , n = 8; NMS- TSP50 fl/fl , n = 6; NMS- TSP50 fl/fl Nestin Cre , n = 6). Values were expressed as means ± SEM. Two‑way ANOVA was used to analyze ( b , c ), one‑way ANOVA was used to analyze ( e , f , g , h ). ns (no significance), * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The sections were incubated with primary antibody: anti-MUC2 (1:1000; ab272692, Abcam, Cambridge, United Kingdom), anti- TSP50 (1:500; 12574-1-AP, Proteintech, Wuhan, China), anti- AChE (1:20; ab2803, Abcam), anti- NESTIN (1:200; 19483-1-AP, Proteintech) and corresponding secondary antibody.

Techniques: Staining, Enzyme-linked Immunosorbent Assay