Journal: PLoS ONE

Article Title: Cyclic ADP Ribose-Dependent Ca 2+ Release by Group I Metabotropic Glutamate Receptors in Acutely Dissociated Rat Hippocampal Neurons

doi: 10.1371/journal.pone.0026625

Figure Lengend Snippet: Acutely dissociated rat hippocampal CA1 neurons were loaded with 2 µM Fura 2-AM for Ca 2+ measurements. Cells were pretreated with 25 µM MPEP (A), 100 µM LY367385 (B), 0 Ca 2+ external solutions (C) or 2 µM thapsigargin (D) prior to the second application of DHPG (50 µM). (E) Bar graphs represent the relative peak amplitude of second Ca 2+ transients to those of first (Ca DHPG,2 /Ca DHPG,1 ). Scale bars indicate 10 sec (horizontal) and 50 nM (vertical). CTRL = control, LY = LY367385, SKF = SKF96365, TG = thapsigargin. ** indicates p <0.01.

Article Snippet: Pipette solutions used for electrophysiology studies contained (in mM) K-gluconate 110, KCl 30, Hepes 20, Mg-ATP 4, Na-vitamin C 4, Na-GTP 0.3, EGTA 0.1 titrated to pH 7.3 with KOH. ( RS )-3,5-DHPG, LY367385, MPEP, SKF96365, CNQX, AP-5, ryanodine, TTX were purchased from Tocris.

Techniques: Control

Journal: BMC Evolutionary Biology

Article Title: Phyloproteomic and functional analyses do not support a split in the genus Borrelia (phylum Spirochaetes)

doi: 10.1186/s12862-019-1379-2

Figure Lengend Snippet: Venn diagrams display shared proteins in the ten most important biological processes (BP) in the species of Spirochaetes targeted in this study. The numbers indicate the mean number of proteins per species involved in each BP. Heat maps included below the Venn diagram of each BP show the Sorensen’s dissimilarity index between the proteins involved in a given process for the different species of bacteria. Rf: Relapsing fever group; LTB: Leptospira spp., Treponema spp., and Brachyspira spp.; Lb: Lyme borreliosis group; FL: free-living species, including the genera Spirochaeta , Sphaerochaeta, and Sediminispirochaeta . The total number of proteins involved in each process is as follows: Carbohydrate metabolism: 263, DNA hydrolysis: 150, DNA repair: 99, Metabolism: 594, Phosphorylation: 358, Redox: 798, Regulation of transcription: 385, Translation: 174, Transmembrane transport: 359, Transport: 711

Article Snippet: Sphaerochaeta coccoides (strain ATCC BAA-1237 / DSM 17374 / SPN1) , FL , UP000007939 , 760,011 , 1819.

Techniques: Bacteria, Phospho-proteomics

Journal: BMC Evolutionary Biology

Article Title: Phyloproteomic and functional analyses do not support a split in the genus Borrelia (phylum Spirochaetes)

doi: 10.1186/s12862-019-1379-2

Figure Lengend Snippet: Detrended Canonical Analysis plot of all biological processes (BPs) found in 41 species of the phylum Spirochaetes displays the similarities between species or strains. Each small black point (unlabeled) represents a BP. The blue points are the species or strains of spirochaetes, placed along the two first axes of the DCA. At the scale shown, the species in the groups Rf and Lb (circled) cannot be separated into individual points; instead, they are all tightly clustered (grouped inside the black circle) and separated from the other taxa. Abbreviations: L: Leptospira , T: Treponema , Br: Brachyspira , Se: Sediminispirochaeta , Sp: Sphaerochaeta , S: Spirochaeta

Article Snippet: Sphaerochaeta coccoides (strain ATCC BAA-1237 / DSM 17374 / SPN1) , FL , UP000007939 , 760,011 , 1819.

Techniques:

Journal: BMC Evolutionary Biology

Article Title: Phyloproteomic and functional analyses do not support a split in the genus Borrelia (phylum Spirochaetes)

doi: 10.1186/s12862-019-1379-2

Figure Lengend Snippet: Phyloproteomic network shows the shared proteins in the 41 species and strains of Spirochaetes. The main four groups of species are Rf: Recurrent fever group; Lb: Lyme borreliosis group; FL: Free life species of the genera Sediminispirochaeta , Spirochaeta and Sphaerochaeta ; and LTB: Leptospira , Treponema , Brachyspira . The small, unlabeled circles represent proteins, and the four large, labeled circles represent the indicated species. The proteins are either clustered around a group of species (unique proteins) or they are linked (shared) with other groups. The links and circles located between groups are color-coded according to the cluster of origin (blue: FL; red: LTB; yellow: Rf and Lb). The size of the circle indicates the weighted degree of importance in the network, which is related to the number of times the protein is shared by the species of Spirochaetes; the smallest points indicate that the protein was only recorded in one group, the largest circles indicate that the protein is shared by all four groups of species

Article Snippet: Sphaerochaeta coccoides (strain ATCC BAA-1237 / DSM 17374 / SPN1) , FL , UP000007939 , 760,011 , 1819.

Techniques: Labeling

Journal: BMC Evolutionary Biology

Article Title: Phyloproteomic and functional analyses do not support a split in the genus Borrelia (phylum Spirochaetes)

doi: 10.1186/s12862-019-1379-2

Figure Lengend Snippet: The list of species and strains included in this study, with reference to the identification number of the proteome and the organism in the UniProt database, as well as the number of proteins annotated

Article Snippet: Sphaerochaeta coccoides (strain ATCC BAA-1237 / DSM 17374 / SPN1) , FL , UP000007939 , 760,011 , 1819.

Techniques:

Journal: Biotech (Basel (Switzerland))

Article Title: Bioinformatics Predicted Linear Epitopes of the Major Coat Protein of the Beet Yellows Virus for Detection of the Virus in the Cell Extract of the Infected Plant.

doi: 10.3390/biotech11040052

Figure Lengend Snippet: Figure 7. Sensitivity of the obtained antisera. (A) antiserum to ep1; (B) antiserum to ep2; (C) antiserum obtained to mix of the epitopes ep1 and ep2. Curves’ numbers show antiserum-antigen pairs analysed: 1, 5, 9—pre-immune sera and native BYV; 2—antiserum to ep1 and ep1; 3—antiserum to ep1 and native BYV; 4—antiserum to ep1 and healthy plant control; 6—antiserum to ep2 and native BYV; 7 antiserum to ep2 and ep2; 8—antiserum to ep2 and healthy plant control; 10—antiserum obtained to the mix of epitopes ep1, ep2 and healthy plant control; 11—antiserum obtained to the mix of epitopes ep1, ep2 and native BYV; 12—antiserum obtained to the mix of epitopes ep1, ep2 and mix of these epitopes.

Article Snippet: Another positive control for the antisera against the BYV was purchased from DSMZ company (Germany).

Techniques: Control

Journal: Biotech (Basel (Switzerland))

Article Title: Bioinformatics Predicted Linear Epitopes of the Major Coat Protein of the Beet Yellows Virus for Detection of the Virus in the Cell Extract of the Infected Plant.

doi: 10.3390/biotech11040052

Figure Lengend Snippet: Figure 6. Dot-ELISA of the murine antisera obtained. The dilution ratios for antisera are indicated on the right, while the quantity of used CP BYV epitope is indicated at the bottom of the figure. (A) antiserum to ep1; (B) antiserum to ep2; (C) antiserum to the mix of ep1 and ep2.

Article Snippet: Another positive control for the antisera against the BYV was purchased from DSMZ company (Germany).

Techniques: Enzyme-linked Immunosorbent Assay