Journal: Stem Cell Research & Therapy

Article Title: Wharton’s jelly mesenchymal stem cells embedded in PF-127 hydrogel plus sodium ascorbyl phosphate combination promote diabetic wound healing in type 2 diabetic rat

doi: 10.1186/s13287-021-02626-w

Figure Lengend Snippet: Immunohistochemistry identifies the macrophage transformation, cell proliferation, and neovascularization. a Immunohistochemical staining with anti-CD86, CD163, Ki-67, and CD31 antibodies on diabetic wound in each group was performed and analyzed at 14 days post-transplantation. Scar bar: 50 μm. b, c The quantitative data of the total number of CD86-positive M1 macrophages ( b ) and CD163-positive M2 macrophages ( c ) per field were analyzed. Error bars represent mean ± SEM; n = 3 independent experiments. Significance was determined using one-way ANOVA. * p < 0.05, *** p < 0.001. d The quantitative data of the percentage of Ki-67-positive proliferating cells per field were analyzed. Error bars represent mean ± SEM; n = 3 independent experiments. Significance was determined using one-way ANOVA. * p < 0.05, *** p < 0.001. e The quantitative data of the total number of CD31-positive newly formed blood vessels were analyzed. Error bars represent mean ± SEM; n = 3 independent experiments. Significance was determined using one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Paraffin sections of each group were undergone antigen retrieval with sodium citrate buffer and blocked by 3% goat serum (16210064, Gibco, USA) for 1 h. Diluted primary antibodies (anti-CD31 antibody (Abcam, ab28364); anti-Ki-67 antibody (Cell Signaling Technology, 12202T); anti-CD163 antibody (Abcam, ab182422); anti-CD86 antibody (Cell Signaling Technology, 19589S)) with blocking buffer were used to incubate at 4 °C overnight.

Techniques: Immunohistochemistry, Transformation Assay, Immunohistochemical staining, Staining, Transplantation Assay

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: CRISPR-Cas9 gene editing of hepatitis B virus in chronically infected humanized mice

doi: 10.1016/j.omtm.2020.11.014

Figure Lengend Snippet: Characterization of HBV-infected and AAV- Sa Cas9-treated humanized FRG mouse livers Serial liver sections from humanized liver FRG mice were stained with hematoxylin and eosin (H&E), subjected to RNAscope for the presence of HBV and Sa Cas9 RNA, or co-labeled by immunohistochemistry for human cytokeratin 18 (hCK18) in combination with HBV surface antigen (HBsAg), activated caspase-3, or Ki67. Representative serial sections are shown for animal GFP-L3, which received control GFP6 and GFP7 sgRNAs. Scale bars, 1 mm.

Article Snippet: For some mice, 5-μm paraffin sections were treated with citrate antigen retrieval buffer after rehydration and stained using antibodies against human FAH (hFAH) (Sigma-Aldrich, AV41681), hCK18 (Agilent Technologies, DC10 #M701029-2), HBsAg (Bio-Rad, OBt0990), Ki67 (Cell Signaling Technology, D3B5 #12202), or cleaved caspase-3-Asp175 (Cell Signaling Technology, 9661).

Techniques: Infection, Staining, RNAscope, Labeling, Immunohistochemistry, Control