11859 freeze dried Search Results


86
Millipore bmp5 prom fw 11859
( A ) Schematic of RNA-seq strategy. mRNA was isolated from 4 days old wild type and Pbsn-Cre Gata3 f/f organoids at passages P0, P2, P3 and P4. ( B ) Deletion of exon four in Pbsn-Cre Gata3 f/f samples increases with passages. Shown are the read counts from RNAseq assigned to the Gata3 locus in samples isolated from wild type and Pbsn-Cre Gata3 f/f prostate tissue at passage P0, P2, P3 or P4. ( C ) Venn diagram of genes differentially expressed between wild type and Pbsn-Cre Gata3 f/f prostate organoids using likelihood-ratio test with q-value <0.01. ( D ) Heatmap of log transformed mRNA read counts of differentially expressed genes between wild type and Pbsn-Cre Gata3 f/f organoids and whose expression pattern follows Gata3 loss with passages. ( E ) <t>Bmp5</t> mRNA expression levels as assessed by quantitative RT-PCR in both wild type and Pbsn-Cre Gata3 f/f organoids over passages. Data represent the average ± SD from three independent cDNA obtained from a pool of prostate cells from a minimum of three mice. Relative mRNA expression levels are normalized to Ppia mRNA levels (two-tailed t-test as compared to wild-type condition; *p<0.01, **p<0.005). See also – . Figure 2—source data 1. Expression levels of differentially expressed genes between wild type and Pbsn-Cre Gata3 f/f associated with . Figure 2—source data 2. Expression value for . Figure 2—source data 3. Statistical analysis for .
Bmp5 Prom Fw 11859, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Chemie GmbH angewandte chemie zuschriften 11859 angew
( A ) Schematic of RNA-seq strategy. mRNA was isolated from 4 days old wild type and Pbsn-Cre Gata3 f/f organoids at passages P0, P2, P3 and P4. ( B ) Deletion of exon four in Pbsn-Cre Gata3 f/f samples increases with passages. Shown are the read counts from RNAseq assigned to the Gata3 locus in samples isolated from wild type and Pbsn-Cre Gata3 f/f prostate tissue at passage P0, P2, P3 or P4. ( C ) Venn diagram of genes differentially expressed between wild type and Pbsn-Cre Gata3 f/f prostate organoids using likelihood-ratio test with q-value <0.01. ( D ) Heatmap of log transformed mRNA read counts of differentially expressed genes between wild type and Pbsn-Cre Gata3 f/f organoids and whose expression pattern follows Gata3 loss with passages. ( E ) <t>Bmp5</t> mRNA expression levels as assessed by quantitative RT-PCR in both wild type and Pbsn-Cre Gata3 f/f organoids over passages. Data represent the average ± SD from three independent cDNA obtained from a pool of prostate cells from a minimum of three mice. Relative mRNA expression levels are normalized to Ppia mRNA levels (two-tailed t-test as compared to wild-type condition; *p<0.01, **p<0.005). See also – . Figure 2—source data 1. Expression levels of differentially expressed genes between wild type and Pbsn-Cre Gata3 f/f associated with . Figure 2—source data 2. Expression value for . Figure 2—source data 3. Statistical analysis for .
Angewandte Chemie Zuschriften 11859 Angew, supplied by Chemie GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Qiagen 11848 11859 2015 blood
( A ) Schematic of RNA-seq strategy. mRNA was isolated from 4 days old wild type and Pbsn-Cre Gata3 f/f organoids at passages P0, P2, P3 and P4. ( B ) Deletion of exon four in Pbsn-Cre Gata3 f/f samples increases with passages. Shown are the read counts from RNAseq assigned to the Gata3 locus in samples isolated from wild type and Pbsn-Cre Gata3 f/f prostate tissue at passage P0, P2, P3 or P4. ( C ) Venn diagram of genes differentially expressed between wild type and Pbsn-Cre Gata3 f/f prostate organoids using likelihood-ratio test with q-value <0.01. ( D ) Heatmap of log transformed mRNA read counts of differentially expressed genes between wild type and Pbsn-Cre Gata3 f/f organoids and whose expression pattern follows Gata3 loss with passages. ( E ) <t>Bmp5</t> mRNA expression levels as assessed by quantitative RT-PCR in both wild type and Pbsn-Cre Gata3 f/f organoids over passages. Data represent the average ± SD from three independent cDNA obtained from a pool of prostate cells from a minimum of three mice. Relative mRNA expression levels are normalized to Ppia mRNA levels (two-tailed t-test as compared to wild-type condition; *p<0.01, **p<0.005). See also – . Figure 2—source data 1. Expression levels of differentially expressed genes between wild type and Pbsn-Cre Gata3 f/f associated with . Figure 2—source data 2. Expression value for . Figure 2—source data 3. Statistical analysis for .
11848 11859 2015 Blood, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech cat 11859 1 ap rrid ab 2065902
( A ) Schematic of RNA-seq strategy. mRNA was isolated from 4 days old wild type and Pbsn-Cre Gata3 f/f organoids at passages P0, P2, P3 and P4. ( B ) Deletion of exon four in Pbsn-Cre Gata3 f/f samples increases with passages. Shown are the read counts from RNAseq assigned to the Gata3 locus in samples isolated from wild type and Pbsn-Cre Gata3 f/f prostate tissue at passage P0, P2, P3 or P4. ( C ) Venn diagram of genes differentially expressed between wild type and Pbsn-Cre Gata3 f/f prostate organoids using likelihood-ratio test with q-value <0.01. ( D ) Heatmap of log transformed mRNA read counts of differentially expressed genes between wild type and Pbsn-Cre Gata3 f/f organoids and whose expression pattern follows Gata3 loss with passages. ( E ) <t>Bmp5</t> mRNA expression levels as assessed by quantitative RT-PCR in both wild type and Pbsn-Cre Gata3 f/f organoids over passages. Data represent the average ± SD from three independent cDNA obtained from a pool of prostate cells from a minimum of three mice. Relative mRNA expression levels are normalized to Ppia mRNA levels (two-tailed t-test as compared to wild-type condition; *p<0.01, **p<0.005). See also – . Figure 2—source data 1. Expression levels of differentially expressed genes between wild type and Pbsn-Cre Gata3 f/f associated with . Figure 2—source data 2. Expression value for . Figure 2—source data 3. Statistical analysis for .
Cat 11859 1 Ap Rrid Ab 2065902, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Kannur corporation 11859 891 n gokul and shanmugam detecting algal blooms 20 74835 153 e
( A ) Schematic of RNA-seq strategy. mRNA was isolated from 4 days old wild type and Pbsn-Cre Gata3 f/f organoids at passages P0, P2, P3 and P4. ( B ) Deletion of exon four in Pbsn-Cre Gata3 f/f samples increases with passages. Shown are the read counts from RNAseq assigned to the Gata3 locus in samples isolated from wild type and Pbsn-Cre Gata3 f/f prostate tissue at passage P0, P2, P3 or P4. ( C ) Venn diagram of genes differentially expressed between wild type and Pbsn-Cre Gata3 f/f prostate organoids using likelihood-ratio test with q-value <0.01. ( D ) Heatmap of log transformed mRNA read counts of differentially expressed genes between wild type and Pbsn-Cre Gata3 f/f organoids and whose expression pattern follows Gata3 loss with passages. ( E ) <t>Bmp5</t> mRNA expression levels as assessed by quantitative RT-PCR in both wild type and Pbsn-Cre Gata3 f/f organoids over passages. Data represent the average ± SD from three independent cDNA obtained from a pool of prostate cells from a minimum of three mice. Relative mRNA expression levels are normalized to Ppia mRNA levels (two-tailed t-test as compared to wild-type condition; *p<0.01, **p<0.005). See also – . Figure 2—source data 1. Expression levels of differentially expressed genes between wild type and Pbsn-Cre Gata3 f/f associated with . Figure 2—source data 2. Expression value for . Figure 2—source data 3. Statistical analysis for .
11859 891 N Gokul And Shanmugam Detecting Algal Blooms 20 74835 153 E, supplied by Kannur corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Schematic of RNA-seq strategy. mRNA was isolated from 4 days old wild type and Pbsn-Cre Gata3 f/f organoids at passages P0, P2, P3 and P4. ( B ) Deletion of exon four in Pbsn-Cre Gata3 f/f samples increases with passages. Shown are the read counts from RNAseq assigned to the Gata3 locus in samples isolated from wild type and Pbsn-Cre Gata3 f/f prostate tissue at passage P0, P2, P3 or P4. ( C ) Venn diagram of genes differentially expressed between wild type and Pbsn-Cre Gata3 f/f prostate organoids using likelihood-ratio test with q-value <0.01. ( D ) Heatmap of log transformed mRNA read counts of differentially expressed genes between wild type and Pbsn-Cre Gata3 f/f organoids and whose expression pattern follows Gata3 loss with passages. ( E ) Bmp5 mRNA expression levels as assessed by quantitative RT-PCR in both wild type and Pbsn-Cre Gata3 f/f organoids over passages. Data represent the average ± SD from three independent cDNA obtained from a pool of prostate cells from a minimum of three mice. Relative mRNA expression levels are normalized to Ppia mRNA levels (two-tailed t-test as compared to wild-type condition; *p<0.01, **p<0.005). See also – . Figure 2—source data 1. Expression levels of differentially expressed genes between wild type and Pbsn-Cre Gata3 f/f associated with . Figure 2—source data 2. Expression value for . Figure 2—source data 3. Statistical analysis for .

Journal: eLife

Article Title: Regulation of stem/progenitor cell maintenance by BMP5 in prostate homeostasis and cancer initiation

doi: 10.7554/eLife.54542

Figure Lengend Snippet: ( A ) Schematic of RNA-seq strategy. mRNA was isolated from 4 days old wild type and Pbsn-Cre Gata3 f/f organoids at passages P0, P2, P3 and P4. ( B ) Deletion of exon four in Pbsn-Cre Gata3 f/f samples increases with passages. Shown are the read counts from RNAseq assigned to the Gata3 locus in samples isolated from wild type and Pbsn-Cre Gata3 f/f prostate tissue at passage P0, P2, P3 or P4. ( C ) Venn diagram of genes differentially expressed between wild type and Pbsn-Cre Gata3 f/f prostate organoids using likelihood-ratio test with q-value <0.01. ( D ) Heatmap of log transformed mRNA read counts of differentially expressed genes between wild type and Pbsn-Cre Gata3 f/f organoids and whose expression pattern follows Gata3 loss with passages. ( E ) Bmp5 mRNA expression levels as assessed by quantitative RT-PCR in both wild type and Pbsn-Cre Gata3 f/f organoids over passages. Data represent the average ± SD from three independent cDNA obtained from a pool of prostate cells from a minimum of three mice. Relative mRNA expression levels are normalized to Ppia mRNA levels (two-tailed t-test as compared to wild-type condition; *p<0.01, **p<0.005). See also – . Figure 2—source data 1. Expression levels of differentially expressed genes between wild type and Pbsn-Cre Gata3 f/f associated with . Figure 2—source data 2. Expression value for . Figure 2—source data 3. Statistical analysis for .

Article Snippet: Sequence-based reagent , Bmp5_prom_Fw+11859 , Sigma-Aldrich , , TTGGAAGAGTTCCGATGAGG.

Techniques: RNA Sequencing Assay, Isolation, Transformation Assay, Expressing, Quantitative RT-PCR, Two Tailed Test

( A–B ) Organoid-forming capacity is abrogated by both BMP and BMPR-SMAD1/5/8 inhibitors treatment. Sorted basal cells from wild type or Krt5 CreERT2 Gata3 f/f prostate were grown in presence or absence of NOGGIN ( A ) or small inhibitor K02288 ( B ) for six passages. Cre activity was induced by hydroxy-tamoxifen treatment in vitro for the first passage ( A ) or by tamoxifen injection in vivo 4 weeks prior to culture ( B ). Organoid-forming potential was assessed as in . ( C ) Bmp5 loss reduces organoid-forming activity in vitro, while propagation potential capacity of wild type cells is increased by BMP5 treatment. Basal cells (10 5 ) from wild type, Bmp5 SE/SE , Krt5 CreERT2 Gata3 f/f or Krt5 CreERT2 Gata3 f/f Bmp5 SE/SE mice were grown for six passages. Exogenous BMP5 was added to culture media where indicated. Cre activity was induced in vivo as in ( B ). ( D ) Bmp5 silencing specifically affects organoid-forming activity in vitro. ShRNA against Bmp5 or scrambled were electroporated in first passage organoid from wild type and Krt5 CreERT2 Gata3 f/f basal cells and grown for seven passages. Cre activity was induced in vivo as in ( B ). ( E ) Bmp5 levels affects regenerative potential in vivo. Different numbers of sorted basal prostate cells from Rosa26 LTdTomato (control) or Bmp5 SE/SE Rosa 26 LTdTomato were transplanted with UGSM either wild type, ectopically expressing BMP5 or derived from Bmp5 SE/SE mice. Limiting dilution analysis was done as in . Notice that the loss of Bmp5 in basal cells but not in UGSM affects prostate reconstituting units (PRU) frequency (two-tailed t-test as compared to wild-type condition; **p<0.02, *p<0.04). ( F ) Immunofluorescence of allografts show presence of prostatic ducts expressing both KRT5 and KRT8/18. Scale bar is representative of 50 μm. See also – . Figure 3—source data 1. Statistical analysis for and . Figure 3—source data 2. Statistical analysis for .

Journal: eLife

Article Title: Regulation of stem/progenitor cell maintenance by BMP5 in prostate homeostasis and cancer initiation

doi: 10.7554/eLife.54542

Figure Lengend Snippet: ( A–B ) Organoid-forming capacity is abrogated by both BMP and BMPR-SMAD1/5/8 inhibitors treatment. Sorted basal cells from wild type or Krt5 CreERT2 Gata3 f/f prostate were grown in presence or absence of NOGGIN ( A ) or small inhibitor K02288 ( B ) for six passages. Cre activity was induced by hydroxy-tamoxifen treatment in vitro for the first passage ( A ) or by tamoxifen injection in vivo 4 weeks prior to culture ( B ). Organoid-forming potential was assessed as in . ( C ) Bmp5 loss reduces organoid-forming activity in vitro, while propagation potential capacity of wild type cells is increased by BMP5 treatment. Basal cells (10 5 ) from wild type, Bmp5 SE/SE , Krt5 CreERT2 Gata3 f/f or Krt5 CreERT2 Gata3 f/f Bmp5 SE/SE mice were grown for six passages. Exogenous BMP5 was added to culture media where indicated. Cre activity was induced in vivo as in ( B ). ( D ) Bmp5 silencing specifically affects organoid-forming activity in vitro. ShRNA against Bmp5 or scrambled were electroporated in first passage organoid from wild type and Krt5 CreERT2 Gata3 f/f basal cells and grown for seven passages. Cre activity was induced in vivo as in ( B ). ( E ) Bmp5 levels affects regenerative potential in vivo. Different numbers of sorted basal prostate cells from Rosa26 LTdTomato (control) or Bmp5 SE/SE Rosa 26 LTdTomato were transplanted with UGSM either wild type, ectopically expressing BMP5 or derived from Bmp5 SE/SE mice. Limiting dilution analysis was done as in . Notice that the loss of Bmp5 in basal cells but not in UGSM affects prostate reconstituting units (PRU) frequency (two-tailed t-test as compared to wild-type condition; **p<0.02, *p<0.04). ( F ) Immunofluorescence of allografts show presence of prostatic ducts expressing both KRT5 and KRT8/18. Scale bar is representative of 50 μm. See also – . Figure 3—source data 1. Statistical analysis for and . Figure 3—source data 2. Statistical analysis for .

Article Snippet: Sequence-based reagent , Bmp5_prom_Fw+11859 , Sigma-Aldrich , , TTGGAAGAGTTCCGATGAGG.

Techniques: Activity Assay, In Vitro, Injection, In Vivo, shRNA, Expressing, Derivative Assay, Two Tailed Test, Immunofluorescence

( A–D ) Growth rate of cells upon organoid passage for the indicated genotypes and treatment calculated from nonlinear regression curve fit of data from , respectively (one-way ANOVA; *p<0.02, **p<0.005, ***p<0.0005, ****p<0.0001). ( E–F ) Treatment of organoids with BMP5 or NOGGIN does not affect proliferation nor apoptosis. Immunofluorescence staining for KRT5 and Ki67 ( E ) or TUNEL reaction ( F ) were done on wild-type 4-day-old organoids treated or not with BMP5 or NOGGIN at passage 2. Scale bar is representative of 50 μm.

Journal: eLife

Article Title: Regulation of stem/progenitor cell maintenance by BMP5 in prostate homeostasis and cancer initiation

doi: 10.7554/eLife.54542

Figure Lengend Snippet: ( A–D ) Growth rate of cells upon organoid passage for the indicated genotypes and treatment calculated from nonlinear regression curve fit of data from , respectively (one-way ANOVA; *p<0.02, **p<0.005, ***p<0.0005, ****p<0.0001). ( E–F ) Treatment of organoids with BMP5 or NOGGIN does not affect proliferation nor apoptosis. Immunofluorescence staining for KRT5 and Ki67 ( E ) or TUNEL reaction ( F ) were done on wild-type 4-day-old organoids treated or not with BMP5 or NOGGIN at passage 2. Scale bar is representative of 50 μm.

Article Snippet: Sequence-based reagent , Bmp5_prom_Fw+11859 , Sigma-Aldrich , , TTGGAAGAGTTCCGATGAGG.

Techniques: Immunofluorescence, Staining, TUNEL Assay

( A ) The aberrant organoid-forming capacity of Pten -deficient basal cells is abrogated by BMP inhibitor (NOGGIN) treatment. Cre activity was induced in vitro by treatment with hydroxy-tamoxifen for the first passage of organoid derived from basal cells of Krt5 CreERT2 Pten f/f mice. From passage 5, organoids were cultured in presence or absence of NOGGIN. Organoid-forming potential was assessed as in . ( B ) Krt5 CreERT2 Pten f/f tamoxifen-treated mice were injected with either K02288 or PBS for 4 weeks. ( C–D ) Representative histological sections of prostate tissue ( C ) and skin tissue ( D ) stained with H&E showing an absence of PIN and skin hyperplasia in K02288-treated as compared to mock-treated mice. ( E ) Kaplan-Meier skin tumor free survival curves of tamoxifen-induced Krt5 CreERT2 Pten f/f treated or not with K02288 (log-rank (Mantel-Cox) test; p<0.0001, n = 7 and n = 14, respectively). Tick-marks represent sacrificed animals. ( F ) Bmp5 loss rescues the aberrant organoid-forming capacity of Pten -deficient basal cells. Cre activity was induced in vivo by tamoxifen injection in adult mice 4 weeks prior to organoid propagation potential assessment. ( G–L ) Eight-week-old mice were treated with tamoxifen and sacrificed 4 weeks later. ( H–I ) Bmp5 loss and Gata3 overexpression rescues Pten -deficient prostate and skin hyperplasia. Shown are representative H&E pictures of prostate ( H ) and skin ( I ) tissues. ( J ) Kaplan-Meier skin tumor free survival curves of tamoxifen-induced Krt5 CreERT2 Pten f/f , Krt5 CreERT2 Pten f/f Bmp5 SE/SE and Krt5 CreERT2 Pten f/f Rosa26 G3/+ mice (log-rank (Mantel-Cox) test; p<0.0001, n = 22, n = 17 and n = 9, respectively). Tick-marks represent sacrificed animals. ( K–L ) Representative FACS phenotype ( K ) and percentage of SCA1 hi cells ( L ) in the luminal compartment as defined by Lin(CD45, CD31, TER119) - EpCAM + CD49f Med of total prostate (one-way ANOVA; ***p<0.0001). ( M ) Representative H&E pictures of prostate tissues of Krt5 CreERT2 Pten f/f and Krt5 CreERT2 Pten f/f Bmp5 SE/SE mice sacrificed 9 weeks after tamoxifen treatment. See also , . Figure 4—source data 1. Statistical analysis for and ; . Figure 4—source data 2. Statistical analysis for . Figure 4—source data 3. Statistical analysis for .

Journal: eLife

Article Title: Regulation of stem/progenitor cell maintenance by BMP5 in prostate homeostasis and cancer initiation

doi: 10.7554/eLife.54542

Figure Lengend Snippet: ( A ) The aberrant organoid-forming capacity of Pten -deficient basal cells is abrogated by BMP inhibitor (NOGGIN) treatment. Cre activity was induced in vitro by treatment with hydroxy-tamoxifen for the first passage of organoid derived from basal cells of Krt5 CreERT2 Pten f/f mice. From passage 5, organoids were cultured in presence or absence of NOGGIN. Organoid-forming potential was assessed as in . ( B ) Krt5 CreERT2 Pten f/f tamoxifen-treated mice were injected with either K02288 or PBS for 4 weeks. ( C–D ) Representative histological sections of prostate tissue ( C ) and skin tissue ( D ) stained with H&E showing an absence of PIN and skin hyperplasia in K02288-treated as compared to mock-treated mice. ( E ) Kaplan-Meier skin tumor free survival curves of tamoxifen-induced Krt5 CreERT2 Pten f/f treated or not with K02288 (log-rank (Mantel-Cox) test; p<0.0001, n = 7 and n = 14, respectively). Tick-marks represent sacrificed animals. ( F ) Bmp5 loss rescues the aberrant organoid-forming capacity of Pten -deficient basal cells. Cre activity was induced in vivo by tamoxifen injection in adult mice 4 weeks prior to organoid propagation potential assessment. ( G–L ) Eight-week-old mice were treated with tamoxifen and sacrificed 4 weeks later. ( H–I ) Bmp5 loss and Gata3 overexpression rescues Pten -deficient prostate and skin hyperplasia. Shown are representative H&E pictures of prostate ( H ) and skin ( I ) tissues. ( J ) Kaplan-Meier skin tumor free survival curves of tamoxifen-induced Krt5 CreERT2 Pten f/f , Krt5 CreERT2 Pten f/f Bmp5 SE/SE and Krt5 CreERT2 Pten f/f Rosa26 G3/+ mice (log-rank (Mantel-Cox) test; p<0.0001, n = 22, n = 17 and n = 9, respectively). Tick-marks represent sacrificed animals. ( K–L ) Representative FACS phenotype ( K ) and percentage of SCA1 hi cells ( L ) in the luminal compartment as defined by Lin(CD45, CD31, TER119) - EpCAM + CD49f Med of total prostate (one-way ANOVA; ***p<0.0001). ( M ) Representative H&E pictures of prostate tissues of Krt5 CreERT2 Pten f/f and Krt5 CreERT2 Pten f/f Bmp5 SE/SE mice sacrificed 9 weeks after tamoxifen treatment. See also , . Figure 4—source data 1. Statistical analysis for and ; . Figure 4—source data 2. Statistical analysis for . Figure 4—source data 3. Statistical analysis for .

Article Snippet: Sequence-based reagent , Bmp5_prom_Fw+11859 , Sigma-Aldrich , , TTGGAAGAGTTCCGATGAGG.

Techniques: Activity Assay, In Vitro, Derivative Assay, Cell Culture, Injection, Staining, In Vivo, Over Expression

Journal: eLife

Article Title: Regulation of stem/progenitor cell maintenance by BMP5 in prostate homeostasis and cancer initiation

doi: 10.7554/eLife.54542

Figure Lengend Snippet:

Article Snippet: Sequence-based reagent , Bmp5_prom_Fw+11859 , Sigma-Aldrich , , TTGGAAGAGTTCCGATGAGG.

Techniques: Transfection, Construct, Recombinant, shRNA, Sequencing, In Situ, Sample Prep, Software