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Image Search Results
Journal: Biochemical Journal
Article Title: Mechanistic studies of the agmatine deiminase from Listeria monocytogenes
doi: 10.1042/BCJ20160221
Figure Lengend Snippet: ( A ) Inactivation of L. monocytogenes AgD by various concentrations of iodoacetamide at pH 7.5: (■) 0 mM, (□) 10 mM, (●) 20 mM and (○) 30 mM iodoacetamide. ( B ) Plot of the pseudo-first-order rate constant of inactivation ( k obs ) against iodoacetamide concentration. ( C ) Plot of k inact / K I against pH identifying the p K a of Cys 356 , i.e. the second-order rate constant of inactivation. ( D ) Substrate protection experiments with L. monocytogenes AgD demonstrates that the substrate can protect against iodoacetamide inactivation.
Article Snippet:
Techniques: Concentration Assay