Journal: Journal of Immunological Methods

Article Title: Preformulation studies with the Escherichia coli double mutant heat-labile toxin adjuvant for use in an oral vaccine

doi: 10.1016/j.jim.2017.09.003

Figure Lengend Snippet: Recovery of intact dmLT after incubation in saliva. dmLT was mixed with either pooled human saliva or DPBS with 0.05% Tween® 80 to achieve a final concentration of 0.2 mg/mL. dmLT dilutions were incubated at either 2 °C–8 °C or 37 °C and samples were taken at 10, 30, and 60 min to test dmLT stability by ELISA (N = 1). DPBS: Dulbecco's phosphate buffered saline.

Article Snippet: For this test, GM1 ganglioside (Sigma-Aldrich, St Louis, MO, cat #G7641), the putative cell receptor for the B-subunit of dmLT, was used to coat 96-well plates (Costar®, Corning®, Corning, NY, cat #9018) by diluting to 1 μg/mL in Dulbecco's phosphate buffered saline (DPBS, pH 7; HyClone™, Fisher Scientific, Waltham, MA, cat #SH30378.02) and adding 0.1 mL/well.

Techniques: Incubation, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Journal of Immunological Methods

Article Title: Preformulation studies with the Escherichia coli double mutant heat-labile toxin adjuvant for use in an oral vaccine

doi: 10.1016/j.jim.2017.09.003

Figure Lengend Snippet: Recovery of dmLT after incubation at pH 3–7. dmLT was mixed to achieve a final concentration of 0.05 mg/mL with 0.01 M citrate buffers containing 0.9% sodium chloride and within a range of pH 3–7 (N = 3). ( A ) pH 3–7 incubation at 2 °C–8 °C; ( B ) pH 3–7 incubation at 37 °C; ( C ) pH 4–5 incubation at 2 °C–8 °C; ( D ) pH 4–5 incubation at 37 °C. Recovery was measured by ELISA. DPBS: Dulbecco's phosphate buffered saline.

Article Snippet: For this test, GM1 ganglioside (Sigma-Aldrich, St Louis, MO, cat #G7641), the putative cell receptor for the B-subunit of dmLT, was used to coat 96-well plates (Costar®, Corning®, Corning, NY, cat #9018) by diluting to 1 μg/mL in Dulbecco's phosphate buffered saline (DPBS, pH 7; HyClone™, Fisher Scientific, Waltham, MA, cat #SH30378.02) and adding 0.1 mL/well.

Techniques: Incubation, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: STAR Protocols

Article Title: Protocol for in vivo elimination of avian auditory hair cells, multiplexed mRNA detection, immunohistochemistry, and S-phase labeling

doi: 10.1016/j.xpro.2024.103118

Figure Lengend Snippet:

Article Snippet: 10X DPBS without Ca 2+ /Mg 2+ , Corning , Cat# 46-013-CM.

Techniques: Sequencing, Recombinant, Fluorsave, Electron Microscopy, Hybridization, Amplification, Software, Adhesive, Microscopy, Sterility, Microinjection, Injection

Journal: STAR Protocols

Article Title: Protocol for in vivo elimination of avian auditory hair cells, multiplexed mRNA detection, immunohistochemistry, and S-phase labeling

doi: 10.1016/j.xpro.2024.103118

Figure Lengend Snippet:

Article Snippet: 10X DPBS without Ca 2+ /Mg 2+ , Corning , Cat# 46-013-CM.

Techniques: Concentration Assay