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Image Search Results
Journal: Molecular Pharmaceutics
Article Title: Role of the Phospholipase A 2 Receptor in Liposome Drug Delivery in Prostate Cancer Cells
doi: 10.1021/mp500174p
Figure Lengend Snippet: Effect of PLA2R1 knockdown on chemotherapeutic-induced toxicity in PC-3 cells. Cells were treated with docetaxel (A), cisplatin (B), or Dox (C) for 72 h. MTT assays were used to determine the effect of knocking down PLA2R1 on chemotherapeutic-induced cytotoxicity. The effect of PLA2R1 on doxorubicin-induced toxicity was further assessed at 72 h using phase contrast microscopy at 10× magnification (D). Data in panels A–C are represented as the mean ± SD of at least three separate experiments ( n = 3/study). Data in panel D are indicative of at least three separate experiments.
Article Snippet:
Techniques: Knockdown, Microscopy
Journal: Molecular Pharmaceutics
Article Title: Role of the Phospholipase A 2 Receptor in Liposome Drug Delivery in Prostate Cancer Cells
doi: 10.1021/mp500174p
Figure Lengend Snippet: Effect of PLA2R1 knockdown on the toxicity of doxorubicin encapsulated in SSL and SPRL in PC-3 cells. PLA2R1 knockdown cells and those expressing scrambled shRNA were treated with 2.5 μM concentrations of Dox encapsulated in SSL or SPRL. MTT assays (A) and phase contrast microscopy at 40× magnification (B) were used to determine the effect of PLA2R1 knockdown on cytotoxicity. Data in panel A are represented as the mean ± SEM of at least three separate experiments ( n = 3/study). *Indicates a significant difference ( p < 0.05) as compared to cells transfected with scrambled shRNA. Data in panel E are indicative of at least three separate experiments.
Article Snippet:
Techniques: Knockdown, Expressing, shRNA, Microscopy, Transfection
Journal: Molecular Pharmaceutics
Article Title: Role of the Phospholipase A 2 Receptor in Liposome Drug Delivery in Prostate Cancer Cells
doi: 10.1021/mp500174p
Figure Lengend Snippet: Effect of PLA2R1 knockdown on DiO and doxorubicin uptake from SSL and SPRL in PC-3 cells. PC-3 cells were treated with liposomes containing Dox and DiO for 24 to 72 h. The efficiency of DiO and drug uptake via SSL (A, B) and SPRL (C, D) was quantified using flow cytometry. Data are represented as the mean ± SEM of at least three separate experiments ( n = 3/study). *Indicates a significant difference ( p < 0.05) as compared to cells expressing scrambled shRNA.
Article Snippet:
Techniques: Knockdown, Liposomes, Flow Cytometry, Expressing, shRNA