Journal: Colloids and surfaces. B, Biointerfaces

Article Title: Adsorption and decontamination of α-synuclein from medically and environmentally-relevant surfaces

doi: 10.1016/j.colsurfb.2018.03.011

Figure Lengend Snippet: Representative AFM images. First row, AFM image of α-syn 10 μg/mL on mica. Similar AFM image result of α-syn on mica was reported in previous study7. Second and third rows, AFM image of fresh stainless steel surface, and representative AFM images of α-syn 10 μg/mL and BSA 10 μg/mL on stainless steel with and without being treated with 1N NaOH. Last row, AFM image of AUT adsorbed on Au surface, and representative AFM images of α-syn 10 μg/mL on AUT surface with and without being treated with 1N NaOH.

Article Snippet: Decontamination/desorption experiments α-Syn and BSA were removed from surfaces with 1N NaOH solution, which was diluted from purified 10N NaOH (Fisher Scientific).

Techniques:

Journal: Colloids and surfaces. B, Biointerfaces

Article Title: Adsorption and decontamination of α-synuclein from medically and environmentally-relevant surfaces

doi: 10.1016/j.colsurfb.2018.03.011

Figure Lengend Snippet: Ex-situ measurements of α-syn and BSA decontamination by 1N NaOH. d and d NaOH indicated the proteins thicknesses before and after being treated with NaOH, respectively.

Article Snippet: Decontamination/desorption experiments α-Syn and BSA were removed from surfaces with 1N NaOH solution, which was diluted from purified 10N NaOH (Fisher Scientific).

Techniques:

Journal: mSystems

Article Title: Commensal Oral Rothia mucilaginosa Produces Enterobactin, a Metal-Chelating Siderophore

doi: 10.1128/mSystems.00161-20

Figure Lengend Snippet: (A) Growth curves for Rothia mucilaginosa ATCC 25296 incubated under aerobic conditions in liquid M9 medium, supplemented with different carbon sources ( x axis, hours; y axis, optical density [OD 600 ]). (B) Colorimetric absorbance at 500 nm ( y axis, A 500 ) capturing catecholate derivatives in liquid R. mucilaginosa (Rmuc) and R. dentocariosa M567 (Rdent) growth cultures ( x axis) using Arnow’s assay. R. mucilaginosa was grown in M9 medium supplemented with 100 mM glycerol (Glyc_Rmuc), 100 mM lactate (Lac_Rmuc), and 100 mM sucrose (Suc_Rmuc). R. dentocariosa was grown in 100 mM glycerol to see if glycerol induced siderophore production as seen for R. mucilaginosa cultures.

Article Snippet: To develop the ruby red color indicating the presence of a catecholate siderophore, 20 μl 10 N NaOH (Ricca Chemical) was added.

Techniques: Incubation

Journal: mSystems

Article Title: Commensal Oral Rothia mucilaginosa Produces Enterobactin, a Metal-Chelating Siderophore

doi: 10.1128/mSystems.00161-20

Figure Lengend Snippet: Replicate mirror mass fragmentation patterns for enterobactin ( m/z 670.152) produced by Rothia mucilaginosa ATCC 25296 (ion fragments pointing up) and enterobactin from the gold standard spectrum (ion fragments pointing down) in the Global Natural Products Social Molecular Networking (GNPS) library . Six major fragments ( m/z 123.04, m/z 137.02, m/z 224.05, m/z 311.09 to 311.14, m/z 447.09 to 447.11, and m/z 534.14) of the query compound matched the gold standard in GNPS. (A) Purified extract derived from R. mucilaginosa ATCC 25296 growth medium (negative ionization mode). (B) Further enrichment of the siderophore using thin-layer chromatography (positive ionization mode). Both experiments confirmed the production of enterobactin.

Article Snippet: To develop the ruby red color indicating the presence of a catecholate siderophore, 20 μl 10 N NaOH (Ricca Chemical) was added.

Techniques: Produced, Purification, Derivative Assay, Thin Layer Chromatography