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Image Search Results
Journal: Life
Article Title: Periostin-Induced Wnt10a Activation Promotes Dental Pulp Stem Cell Migration During Pulp Regeneration
doi: 10.3390/life15111732
Figure Lengend Snippet: Wnt10a expression rate in migrated cells. ( A ) Double stained images of migrated cells with Wnt10a immunostaining (red) and hoechst33342 immunostaining (blue). ( a – c ) negative control; neg. ( d – f ) positive control; pog(FBS). ( g – i ) DKK1. ( a , d , g ) Wnt10a immunostained images. ( b , e , h ) hoechst33342 immunostained image. ( c , f , i ) merged images. ( B ) Wnt10a positive cell rate of migrated cells. ( C ) Fluorescence insensitivity. * p < 0.05, ** p < 0.01. All data represent mean ± standard deviation (n = 4).
Article Snippet: The primary antibody was anti
Techniques: Expressing, Staining, Immunostaining, Negative Control, Positive Control, Fluorescence, Standard Deviation
Journal: Life
Article Title: Periostin-Induced Wnt10a Activation Promotes Dental Pulp Stem Cell Migration During Pulp Regeneration
doi: 10.3390/life15111732
Figure Lengend Snippet: Localization and Gene Expression of Migrated Cells in Regenerated Dental Pulp. ( A ) HE stained image ( a , d ). Immunostained images of Regenerated pulp with BrdU ( b , e ), Wnt10a ( c , f ). ( B ) Double stained images of regenerated pulp-like tissue with Hoechst 33342 immunostaining (blue), BrdU immunostaining (green), and Wnt10a immunostaining (red). ( a ) Hoechst 33342 immunostaining. ( b ) BrdU immunostaining. ( c ) Wnt10a immunostaining. ( d ) merged images of Hoechst 33342, BrdU and Wnt10a. ( C ) Gene Expression in dental pulp. ( a ) Wnt10a, ( b ) DKK1, ( c ) LRP5/6, ( d ) Periostin (POSTN). ( D ) Foldchange values and statistical significance. * p < 0.05, ** p < 0.01. Relative quantification by ΔΔCt method after q-PCR analysis. All data represent mean ± standard deviation (n = 4).
Article Snippet: The primary antibody was anti
Techniques: Gene Expression, Staining, Immunostaining, Quantitative Proteomics, Standard Deviation
Journal: Life
Article Title: Periostin-Induced Wnt10a Activation Promotes Dental Pulp Stem Cell Migration During Pulp Regeneration
doi: 10.3390/life15111732
Figure Lengend Snippet: Changes in the number of migrated cells and the rate of Wnt10a-positive cells induced by periostin stimulation. ( A ) Double stained images of regenerated pulp-like tissue with Wnt10a immunostaining (red) and hoechst33342 immunostaining (blue). ( a – c ) neg. ( d – f ) POSTN. ( g – i ) DKK1. ( j – l ) DKK1 and POSTN. ( a , d , g , j ) Wnt10a immunostained images. ( b , e , h , k ) Hoechst33342 immunostained image. ( c , f , i , l ) superimposed images of Wnt10a and hoechst33342. ( B ) Double stained images of regenerated pulp-like tissue with Wnt10a immunostaining (red) and Lectin immunostaining (green). ( a – d ) Wnt10a immunostained images. ( e – h ) Lectin immunostained image. ( i – l ) superimposed images of Wnt10a and Lectin. ( a , e , i ) 3 days. ( b , f , j ) 7 days. ( c , g , k ) 14 days. ( d , h , l ) 21 days. ( C ) Area of angiogenesis within the regenerated pulp-like tissue. * p < 0.05. Relative quantification by ΔΔCt method after q-PCR analysis. All data represent mean ± standard deviation (n = 4).
Article Snippet: The primary antibody was anti
Techniques: Staining, Immunostaining, Quantitative Proteomics, Standard Deviation
Journal: Life
Article Title: Periostin-Induced Wnt10a Activation Promotes Dental Pulp Stem Cell Migration During Pulp Regeneration
doi: 10.3390/life15111732
Figure Lengend Snippet: Comparison of Wnt10a Expression Levels in Periostin-Stimulated Cells. ( A ) Gene Expression in Periostin-Stimulated Cells. ( a ) Wnt10a, ( b ) DKK1, ( c ) LRP5/6, ( d ) Periostin(POSTN). ( B ) Foldchange values and statistical significance. ( C ) Wnt10a Expression in Periostin-Stimulated Cells by ELISA. ( D ) Wnt10a Expression in Periostin-Stimulated Cells by Western blotting. * p < 0.05. Relative quantification by ΔΔCt method after q-PCR analysis. All data represent mean ± standard deviation (n = 4).
Article Snippet: The primary antibody was anti
Techniques: Comparison, Expressing, Gene Expression, Enzyme-linked Immunosorbent Assay, Western Blot, Quantitative Proteomics, Standard Deviation