10829448 Search Results


p101  (Cell Signaling Technology Inc)
92
Cell Signaling Technology Inc p101
( A ) p110α lipid kinase activity, ( B ) p110β lipid kinase activity, and ( C ) IRS1-associated phosphatidylinositol (PI) kinase activity in L-DKO mice and controls were assessed by immunoprecipitation. ( D ) Representative western blot of immunoprecipitation experiments with antibodies for IRS1 and subsequent immunoblotting with antibodies for p110γ, <t>p101,</t> Vps34, p150, PIK3-C2α and PIK3-C2γ. The whole-lysate reference sample was from an insulin-treated flox control mouse. IP = immunoprecipitation, IB = immunoblot. Basal condition is indicated with a minus (-) sign, insulin-treated condition is indicated with a plus (+) sign. Basal conditions refer to fasting of mice overnight and injection of saline through the vena cava. Insulin treatment refers to injection of 5 U of insulin through the vena cava 5 min prior to euthanization. Error bars indicate SEM (n = 4). *, p < 0.05 compared to controls.
P101, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p101/product/Cell Signaling Technology Inc
Average 92 stars, based on 1 article reviews
p101 - by Bioz Stars, 2026-02
92/100 stars
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rp24-66j14  (ATCC)
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( A ) p110α lipid kinase activity, ( B ) p110β lipid kinase activity, and ( C ) IRS1-associated phosphatidylinositol (PI) kinase activity in L-DKO mice and controls were assessed by immunoprecipitation. ( D ) Representative western blot of immunoprecipitation experiments with antibodies for IRS1 and subsequent immunoblotting with antibodies for p110γ, p101, Vps34, p150, PIK3-C2α and PIK3-C2γ. The whole-lysate reference sample was from an insulin-treated flox control mouse. IP = immunoprecipitation, IB = immunoblot. Basal condition is indicated with a minus (-) sign, insulin-treated condition is indicated with a plus (+) sign. Basal conditions refer to fasting of mice overnight and injection of saline through the vena cava. Insulin treatment refers to injection of 5 U of insulin through the vena cava 5 min prior to euthanization. Error bars indicate SEM (n = 4). *, p < 0.05 compared to controls.

Journal: F1000Research

Article Title: Hepatic deletion of p110α and p85α results in insulin resistance despite sustained IRS1-associated phosphatidylinositol kinase activity

doi: 10.12688/f1000research.12418.2

Figure Lengend Snippet: ( A ) p110α lipid kinase activity, ( B ) p110β lipid kinase activity, and ( C ) IRS1-associated phosphatidylinositol (PI) kinase activity in L-DKO mice and controls were assessed by immunoprecipitation. ( D ) Representative western blot of immunoprecipitation experiments with antibodies for IRS1 and subsequent immunoblotting with antibodies for p110γ, p101, Vps34, p150, PIK3-C2α and PIK3-C2γ. The whole-lysate reference sample was from an insulin-treated flox control mouse. IP = immunoprecipitation, IB = immunoblot. Basal condition is indicated with a minus (-) sign, insulin-treated condition is indicated with a plus (+) sign. Basal conditions refer to fasting of mice overnight and injection of saline through the vena cava. Insulin treatment refers to injection of 5 U of insulin through the vena cava 5 min prior to euthanization. Error bars indicate SEM (n = 4). *, p < 0.05 compared to controls.

Article Snippet: IRS1 (RRID:AB_2127860, rabbit monoclonal, 1:50, Cell Signaling Technology Inc, cat# 3407 for western blot), IRS1 (RRID:AB_631842, rabbit polyclonal, 10μl per reaction, Santa Cruz Biotechnology Inc, cat# sc-559 for immunoprecipitation), p110α (rabbit monoclonal, 1:1000 for western blot and 1:50 for immunoprecipitation, Cell Signaling Technology Inc, cat# 4249), p110β (RRID:AB_2165246, rabbit monoclonal, 1:500 for western blot and 1:50 for immunoprecipitation, Cell Signaling Technology Inc, cat# 3011), p110γ (RRID:AB_10828316, rabbit monoclonal, 1:1000, Cell Signaling Technology Inc, cat# 5405), p110δ (mouse monoclonal, 1:500, Becton, Dickinson and Company, cat# 611015), p101 (RRID:AB_10829448, rabbit monoclonal, 1:1000, Cell Signaling Technology Inc, cat# 5569), Vps34 (RRID:AB_2299765, rabbit monoclonal, 1:1000, Cell Signaling Technology Inc, cat# 4263), p150 (rabbit polyclonal, 1:1000, Cell Signaling Technology Inc, cat# 14580), Akt/PKB (RRID:AB_329827, rabbit polyclonal, 1:1000, Cell Signaling Technology Inc, cat# 9272), pS-Akt/PKB (RRID:AB_329825, rabbit polyclonal, 1:1000, Cell Signaling Technology Inc, cat# 9271), pT-Akt/PKB (RRID:AB_2255933, rabbit monoclonal, 1:1000, Cell Signaling Technology Inc, cat# 2965), pT-p70S6K (RRID:AB_330944, rabbit polyclonal, 1:1000, Cell Signaling Technology Inc, cat# 9205), p70S6K (rabbit polyclonal, 1:500, Cell Signaling Technology Inc, cat# 9202), p85α (RRID:AB_2268174, rabbit monoclonal, 1:1000, Abcam, cat# 22653), p85-pan (RRID:AB_10831521, rabbit monoclonal, 1:1000, Cell Signaling Technology Inc, cat# 4257), pT202/Y204-ERK (RRID:AB_2315112, rabbit monoclonal, 1:1000, Cell Signaling Technology Inc, cat# 4370), ERK (RRID:AB_390779, rabbit monoclonal, 1:1000, Cell Signaling Technology Inc, cat# 4695), p55γ (mouse monoclonal, 1:2000, Abcam, cat# ab186612), PIK3C2α (rabbit polyclonal, 1:1000, MyBiosource, cat# MBS9202698), PIK3C2γ (rabbit polyclonal, 1:1000, MyBiosource, cat# MBS820611).

Techniques: Activity Assay, Immunoprecipitation, Western Blot, Control, Injection, Saline