Journal:

Article Title: The Mitochondrial Toxin Produced by Streptomyces griseus Strains Isolated from an Indoor Environment Is Valinomycin

doi:

Figure Lengend Snippet: Toxicity to boar spermatozoa of methanol-soluble substances from actinomycetes isolated from indoor environments

Article Snippet: Twelve strains were identified by chemotaxonomic methods and by 16S rDNA sequencing as members of the genera Streptomyces , Nocardiopsis , and Dietzia (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain Toxicity to boar spermatozoa a Source of strain b EC 50 of methanol-soluble substances (ng ml −1 ) EC 50 of cell extract (mg ml −1 ) Indoor isolates S. griseus 2/ppi 20 0.0005 (10 5 ) c Children’s day care center (settled dust) S. griseus 8/ppi 50 0.001 (10 6 ) Children’s day care center (settled dust) S. griseus 10/ppi 10 0.0009 (10 6 ) Children’s day care center (settled dust) S. griseus 1/k 63 0.002 (10 6 ) Elementary school (air) Nocardiopsis albus 123 7,500 0.5 (10 8 ) Children’s day care center (water-damaged building material) S. griseus 157 59,000 >9 (>10 9 ) Children’s day care center (water-damaged building material) Dietzia sp. strain 147 >72,000 >1 (>10 9 ) Children’s day care center (water-damaged building material) Dietzia sp. strain 148 >86,000 >1 (>10 9 ) Children’s day care center (water-damaged building material) Nocardiopsis dassonvillei 305 >10,000 >0.3 (>10 8 ) Cattle barn (air) Streptomyces albidoflavus 703 36,000 1 (10 8 ) Cattle barn (settled dust) Nocardiopsis dassonvillei 704 >40,000 >0.5 (>10 8 ) Cattle barn (air) Reference strains S. griseus DSM 40236 T 26,000 1 (10 8 ) DSMZ Dietzia maris N 1009 T <36,000 >1 (>10 8 ) DSMZ Bacillus mycoides ATCC 6462 T 67,000 2.5 (10 8 ) ATCC B. cereus ATCC 14569 T 16,000 1 (10 8 ) ATCC B. cereus 4810/72 c 28 0.002 (10 6 ) Food poisoning (emetic) Open in a separate window a The EC 50 is the concentration that paralyzed more than 50% of the sperm cells. b DSMZ, Deutsche Sammlung von Mikroorganism und Zellkulturen GmbH; ATCC, American Type Culture Collection. c The numbers in parentheses are the equivalent numbers of cells. d Data from reference 3 .

Techniques: Isolation