Journal: Journal of medical virology

Article Title: Bidirectional transfer of human cytomegalovirus strains in donor and recipient seropositive lung transplant patients.

doi: 10.1002/jmv.29770

Figure Lengend Snippet: FIGURE 1 HCMV genotypes present in pre‐Tx donor (D) and recipient (R) lung tissue and post‐Tx emergence in D + R + LTR. (A) HCMV DNA loads in pre‐Tx D and R lung FFPE sections, in post‐Tx follow‐up plasma and BAL samples, and in the cellular subsets of the BAL. Red circles indicate samples that underwent HCMV genotyping. Gray bars show the period under antiviral therapy. (B) CD45 + BAL cells were further sorted for CD163 and CD14 expression, distinguishing between CD14 + CD163‐ (left upper gate), CD14 + CD163+ (right upper gate) and CD14−cells (left lower gate). Red squares indicate HCMV‐DNA positive cell subsets. (C) Schematic illustration of the D and R FFPE blocks with the number of sections investigated. HCMV‐DNA positive slides are dark green and red circles indicate the slides positive for HCMV genotyping. (D) Relative frequency of the genotype‐specific reads in the indicated post‐Tx samples. BAL, bronchoalveolar lavage; d, day; FFPE, formalin‐fixed paraffin‐embedded; gN, gO, glycoprotein N, O; LTR, lung transplant recipient; Tx, transplantation; UL, unique long.

Article Snippet: From the FFPE tissue sections DNA was extracted using the FavorPrep FFPE DNA Extraction Micro Kit (Favorgen) according to manufacturer's protocol, while DNA was eluted with 36 μL of preheated elution buffer giving about 30 μL final volume.

Techniques: Clinical Proteomics, Expressing, Formalin-fixed Paraffin-Embedded, Transplantation Assay

Journal: Journal of medical virology

Article Title: Bidirectional transfer of human cytomegalovirus strains in donor and recipient seropositive lung transplant patients.

doi: 10.1002/jmv.29770

Figure Lengend Snippet: FIGURE 2 HCMV genotypes present in pre‐Tx recipient lung tissue and post‐Tx emergence in D + R + LTR. (A) HCMV DNA loads in R pre‐Tx lung FFPE sections, in post‐Tx follow‐up plasma and BAL samples, and in the cellular subsets of the BAL. Red circles indicate samples that underwent HCMV genotyping. Gray bars show the period under antiviral therapy. (B) CD45 + BAL cells were further sorted for CD163 and CD14 expression, distinguishing between CD14 + CD163−(left upper gate), CD14+ CD163+ (right upper gate) and CD14−cells (left lower gate). Red squares indicate HCMV‐DNA positive cell subsets. (C) Schematic illustration of the D and R FFPE blocks with number of sections investigated. HCMV‐DNA positive slides are dark green and red circles indicate the slides positive for HCMV genotyping. (D) Relative frequency of the genotype‐specific reads in the indicated post‐Tx samples. BAL, bronchoalveolar lavage; d, day; D, donor; FFPE, formalin‐fixed paraffin‐ embedded; gN, gO, glycoprotein N, O; LTR, lung transplant recipient; R, recipient; Tx, transplantation; UL, unique long.

Article Snippet: From the FFPE tissue sections DNA was extracted using the FavorPrep FFPE DNA Extraction Micro Kit (Favorgen) according to manufacturer's protocol, while DNA was eluted with 36 μL of preheated elution buffer giving about 30 μL final volume.

Techniques: Clinical Proteomics, Expressing, Formalin-fixed Paraffin-Embedded, Transplantation Assay