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Image Search Results
Journal: International Journal of Biological Sciences
Article Title: NVP-BEZ235 inhibits thyroid cancer growth by p53- dependent/independent p21 upregulation
doi: 10.7150/ijbs.37592
Figure Lengend Snippet: NVP-BEZ235 induces cell cycle arrest in thyroid cancer cells. (A) IHH4, K1, BCPAP and C643 cells were treated with 200 nM NVP-BEZ235 for 48 hours, respectively. DNA content was measured by flow cytometry to determine cell cycle fractions. The fraction of cells in each cell cycle phase was indicated in the figures. (B) The protein expressions of cyclin E1 and cyclin-dependent kinase 2 (Cdk2) were examined by western blot in IHH4, K1, BCPAP and C643 cells with 200 nM NVP-BEZ235 treatment (left). Relative protein levels were quantified by ImageJ software (right). * p < 0.05, ** p < 0.01, *** p < 0.001, respectively, NVP-BEZ235 versus control.
Article Snippet:
Techniques: Flow Cytometry, Western Blot, Software
Journal: International Journal of Biological Sciences
Article Title: NVP-BEZ235 inhibits thyroid cancer growth by p53- dependent/independent p21 upregulation
doi: 10.7150/ijbs.37592
Figure Lengend Snippet: p21 knockdown abolished the effect of NVP-BEZ235 in both p53 wild type and mutant thyroid cancer cells. ( A) MTT assays showed that the inhibitory effect of cell growth on IHH4, K1, BCPAP and C643 cells after NVP-BEZ235 treatment (200 nM) was abolished by p21 knockdown (si-p21). (B) Cell cycle arrest induced by NVP-BEZ235 (200 nM) was reversed by p21 knockdown (si-p21) in IHH4, K1, BCPAP and C643 cell lines. (C) p21 knockdown (si-p21) was performed in IHH4, K1, BCPAP and C643 cells and then cells were treated with NVP-BEZ235. The protein expressions of p21, cyclin E1 and Cdk2 were determined by Western blot (left). Relative protein levels were quantified by ImageJ software (right). GAPDH was used as loading control. 235, NVP-BEZ235; NC, Negative control. * p < 0.05, ** p < 0.01, *** p < 0.001, respectively, 235 + si-p21 versus 235 + NC.
Article Snippet:
Techniques: Mutagenesis, Western Blot, Software, Negative Control