10 ng Search Results


94
Chem Impex International triethanolamine
Triethanolamine, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ng Abemaxp2a Gfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PeproTech g-csf (10 ng/ml)
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40 Ng/Ml Egf, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genzyme 10 ng/ml scf
10 Ng/Ml Scf, supplied by Genzyme, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PeproTech 10 ng/ml gdnf
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PeproTech 10 ng/ml recombinant murine il-3
(A) Indicated numbers of in <t>vitro</t> <t>IL-3-induced</t> bone marrow B6 basophils (DX5+) or GM-CSF-induced bone marrow dendritic cells (DCs, CD11c+) and 4×105 BALB/c CD90+ T cells were co-cultured in 96-well round bottom plate for 5 days. Incorporation of 3H-thymidine (1 μCi/well) by proliferating cells during the last 6 h of culture was measured. (B) Indicated numbers of in vitro IL-3-induced BALB/c bone marrow basophils (DX5+) or GM-CSF-induced bone marrow dendritic cells (CD11c+) and 4×105 B6 CD90+ T cells were co-cultured in 96-well round bottom plate for 5 days. Incorporation of 3H-thymidine (1 μCi/well) by proliferating cells during the last 6 h of culture was measured. (C) Flow cytometric analysis of B6 and BALB/c bone marrow DCs and basophils. MHC class II (I-Ab for B6, I-Ad/I-Ed for BALB/c), CD40, CD80 and CD86 expression levels on CD11c+ cells (DCs, top row) and DX5+ cells (basophils, bottom row) are shown. Numbers shown in each histogram indicate % positivity of the surface marker. (D) Flow cytometric analysis of control hamster mAb (left) or MAR-1 (right) treated BALB/c mouse peripheral blood. Mice were treated with 10 μg of control mAb or MAR-1 three times every 24 hours and peripheral blood samples were corrected from the mice and analyzed. Numbers shown in dot plots indicate % of DX5+FcεRI+ cells.
10 Ng/Ml Recombinant Murine Il 3, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ProSpec 10 ng/ml human recombinant il-4
(A) Indicated numbers of in <t>vitro</t> <t>IL-3-induced</t> bone marrow B6 basophils (DX5+) or GM-CSF-induced bone marrow dendritic cells (DCs, CD11c+) and 4×105 BALB/c CD90+ T cells were co-cultured in 96-well round bottom plate for 5 days. Incorporation of 3H-thymidine (1 μCi/well) by proliferating cells during the last 6 h of culture was measured. (B) Indicated numbers of in vitro IL-3-induced BALB/c bone marrow basophils (DX5+) or GM-CSF-induced bone marrow dendritic cells (CD11c+) and 4×105 B6 CD90+ T cells were co-cultured in 96-well round bottom plate for 5 days. Incorporation of 3H-thymidine (1 μCi/well) by proliferating cells during the last 6 h of culture was measured. (C) Flow cytometric analysis of B6 and BALB/c bone marrow DCs and basophils. MHC class II (I-Ab for B6, I-Ad/I-Ed for BALB/c), CD40, CD80 and CD86 expression levels on CD11c+ cells (DCs, top row) and DX5+ cells (basophils, bottom row) are shown. Numbers shown in each histogram indicate % positivity of the surface marker. (D) Flow cytometric analysis of control hamster mAb (left) or MAR-1 (right) treated BALB/c mouse peripheral blood. Mice were treated with 10 μg of control mAb or MAR-1 three times every 24 hours and peripheral blood samples were corrected from the mice and analyzed. Numbers shown in dot plots indicate % of DX5+FcεRI+ cells.
10 Ng/Ml Human Recombinant Il 4, supplied by ProSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
PeproTech neurotrophin-3 10 ng/ml
(A) Indicated numbers of in <t>vitro</t> <t>IL-3-induced</t> bone marrow B6 basophils (DX5+) or GM-CSF-induced bone marrow dendritic cells (DCs, CD11c+) and 4×105 BALB/c CD90+ T cells were co-cultured in 96-well round bottom plate for 5 days. Incorporation of 3H-thymidine (1 μCi/well) by proliferating cells during the last 6 h of culture was measured. (B) Indicated numbers of in vitro IL-3-induced BALB/c bone marrow basophils (DX5+) or GM-CSF-induced bone marrow dendritic cells (CD11c+) and 4×105 B6 CD90+ T cells were co-cultured in 96-well round bottom plate for 5 days. Incorporation of 3H-thymidine (1 μCi/well) by proliferating cells during the last 6 h of culture was measured. (C) Flow cytometric analysis of B6 and BALB/c bone marrow DCs and basophils. MHC class II (I-Ab for B6, I-Ad/I-Ed for BALB/c), CD40, CD80 and CD86 expression levels on CD11c+ cells (DCs, top row) and DX5+ cells (basophils, bottom row) are shown. Numbers shown in each histogram indicate % positivity of the surface marker. (D) Flow cytometric analysis of control hamster mAb (left) or MAR-1 (right) treated BALB/c mouse peripheral blood. Mice were treated with 10 μg of control mAb or MAR-1 three times every 24 hours and peripheral blood samples were corrected from the mice and analyzed. Numbers shown in dot plots indicate % of DX5+FcεRI+ cells.
Neurotrophin 3 10 Ng/Ml, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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PeproTech 10 ng ml−1 of lps
(A) Indicated numbers of in <t>vitro</t> <t>IL-3-induced</t> bone marrow B6 basophils (DX5+) or GM-CSF-induced bone marrow dendritic cells (DCs, CD11c+) and 4×105 BALB/c CD90+ T cells were co-cultured in 96-well round bottom plate for 5 days. Incorporation of 3H-thymidine (1 μCi/well) by proliferating cells during the last 6 h of culture was measured. (B) Indicated numbers of in vitro IL-3-induced BALB/c bone marrow basophils (DX5+) or GM-CSF-induced bone marrow dendritic cells (CD11c+) and 4×105 B6 CD90+ T cells were co-cultured in 96-well round bottom plate for 5 days. Incorporation of 3H-thymidine (1 μCi/well) by proliferating cells during the last 6 h of culture was measured. (C) Flow cytometric analysis of B6 and BALB/c bone marrow DCs and basophils. MHC class II (I-Ab for B6, I-Ad/I-Ed for BALB/c), CD40, CD80 and CD86 expression levels on CD11c+ cells (DCs, top row) and DX5+ cells (basophils, bottom row) are shown. Numbers shown in each histogram indicate % positivity of the surface marker. (D) Flow cytometric analysis of control hamster mAb (left) or MAR-1 (right) treated BALB/c mouse peripheral blood. Mice were treated with 10 μg of control mAb or MAR-1 three times every 24 hours and peripheral blood samples were corrected from the mice and analyzed. Numbers shown in dot plots indicate % of DX5+FcεRI+ cells.
10 Ng Ml−1 Of Lps, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Indicated numbers of in vitro IL-3-induced bone marrow B6 basophils (DX5+) or GM-CSF-induced bone marrow dendritic cells (DCs, CD11c+) and 4×105 BALB/c CD90+ T cells were co-cultured in 96-well round bottom plate for 5 days. Incorporation of 3H-thymidine (1 μCi/well) by proliferating cells during the last 6 h of culture was measured. (B) Indicated numbers of in vitro IL-3-induced BALB/c bone marrow basophils (DX5+) or GM-CSF-induced bone marrow dendritic cells (CD11c+) and 4×105 B6 CD90+ T cells were co-cultured in 96-well round bottom plate for 5 days. Incorporation of 3H-thymidine (1 μCi/well) by proliferating cells during the last 6 h of culture was measured. (C) Flow cytometric analysis of B6 and BALB/c bone marrow DCs and basophils. MHC class II (I-Ab for B6, I-Ad/I-Ed for BALB/c), CD40, CD80 and CD86 expression levels on CD11c+ cells (DCs, top row) and DX5+ cells (basophils, bottom row) are shown. Numbers shown in each histogram indicate % positivity of the surface marker. (D) Flow cytometric analysis of control hamster mAb (left) or MAR-1 (right) treated BALB/c mouse peripheral blood. Mice were treated with 10 μg of control mAb or MAR-1 three times every 24 hours and peripheral blood samples were corrected from the mice and analyzed. Numbers shown in dot plots indicate % of DX5+FcεRI+ cells.

Journal: Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Article Title: Host Basophils are Dispensable for Induction of Donor Th2 Differentiation and Severity of Experimental Graft-versus-Host Disease

doi: 10.1016/j.bbmt.2011.08.013

Figure Lengend Snippet: (A) Indicated numbers of in vitro IL-3-induced bone marrow B6 basophils (DX5+) or GM-CSF-induced bone marrow dendritic cells (DCs, CD11c+) and 4×105 BALB/c CD90+ T cells were co-cultured in 96-well round bottom plate for 5 days. Incorporation of 3H-thymidine (1 μCi/well) by proliferating cells during the last 6 h of culture was measured. (B) Indicated numbers of in vitro IL-3-induced BALB/c bone marrow basophils (DX5+) or GM-CSF-induced bone marrow dendritic cells (CD11c+) and 4×105 B6 CD90+ T cells were co-cultured in 96-well round bottom plate for 5 days. Incorporation of 3H-thymidine (1 μCi/well) by proliferating cells during the last 6 h of culture was measured. (C) Flow cytometric analysis of B6 and BALB/c bone marrow DCs and basophils. MHC class II (I-Ab for B6, I-Ad/I-Ed for BALB/c), CD40, CD80 and CD86 expression levels on CD11c+ cells (DCs, top row) and DX5+ cells (basophils, bottom row) are shown. Numbers shown in each histogram indicate % positivity of the surface marker. (D) Flow cytometric analysis of control hamster mAb (left) or MAR-1 (right) treated BALB/c mouse peripheral blood. Mice were treated with 10 μg of control mAb or MAR-1 three times every 24 hours and peripheral blood samples were corrected from the mice and analyzed. Numbers shown in dot plots indicate % of DX5+FcεRI+ cells.

Article Snippet: For the generation of bone marrow basophils, bone marrow cells were cultured for 9–10 days in RPMI1640 media supplemented with 10% FCS and 10 ng/ml recombinant murine IL-3 (Peprotech) 1 , 6 , 8 , 9 .

Techniques: In Vitro, Cell Culture, Expressing, Marker