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Image Search Results
Journal: Molecular Neurobiology
Article Title: Transplantation of Wnt5a-modified Bone Marrow Mesenchymal Stem Cells Promotes Recovery After Spinal Cord Injury via the PI3K/AKT Pathway
doi: 10.1007/s12035-024-04248-8
Figure Lengend Snippet: In vivo transplantation of Wnt5a-modified BMSCs reduces astrocyte production and promotes neuronal regeneration. A - C : Immunofluorescence staining of GAP43, MAP2, and MBP-positive cells in spinal cord tissues. Data are presented as mean ± SEM. # P <0:05 compared to the SCI group; * P <0:05 compared to the NC group. D : GAP43, MAP2, GFAP, and MBP protein expression were measured in spine tissue samples by western blotting. Data are presented as mean ± SEM. & P <0:05 compared to the Sham group; # P <0:05 compared with SCI group; * P <0:05 compared to NC group
Article Snippet: The membrane was sealed using pre-chilled NcmBlot Rapid Closure Solution, and primary antibodies were added at 4°C: microtubule-associated protein-2 (MAP-2) (1:1000; Boster Biological Engineering Co.), glial fibrillary acidic protein (GFAP) (1:1000; Boster Biological Engineering Co.), β3-tubulin (1:1000; CST), growth-associated protein 43 (GAP43) (1:1000; NOVUS),
Techniques: In Vivo, Transplantation Assay, Modification, Immunofluorescence, Staining, Expressing, Western Blot