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Image Search Results
Journal: International Journal of Cancer
Article Title: Crumbs3 is a critical factor that regulates invasion and metastasis of colon adenocarcinoma via the specific interaction with FGFR1
doi: 10.1002/ijc.32336
Figure Lengend Snippet: FGFR1 correlates with Crb3a in colon cancer cells. ( a , d ) Knockdown of FGFR1 or FGFR4 using siRNAs in ( a ) DLD‐1 or ( d ) WiDr cells were validated by immunoblot. ( b , e ) MTT assay using siFGFR treated ( b ) DLD‐1 or ( e ) WiDr cells. ( c ) Cell migration of siFGFR1 treated DLD‐1 cells was examined by transwell chamber assay. ( f ) Expression of FLAG‐Crb3a, FGFR1‐HIS, FGFR1Δcyto‐HIS and Tubulin in stably expressing cells was validated by immunoblot. ( g ) Cell migration of Crb3 ‐KO DLD‐1 cell stably expressing Crb3 and/or FGFR1 constructions was evaluated by transwell chamber assay. ( h ) Cell migration of Crb3a ‐revertant DLD‐1 cells was examined by transwell chamber assay in the presence of 20 μM MEK inhibitors (SL327 or U0126). ( i ) ERK phosphorylation by recombinant FGF1 treatment decreased in Crb3 ‐KO DLD‐1 cells, and forced expression of Crb3a in Crb3 ‐KO cell recovered ERK phosphorylation.
Article Snippet: Stimulation was begun by switching opti‐MEMI including 100 ng/mL
Techniques: Western Blot, MTT Assay, Migration, Transwell Chamber Assay, Expressing, Stable Transfection, Recombinant