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Journal: Biomolecules
Article Title: Direct Effects of Capsaicin on Voltage-Dependent Calcium Channels of Mammalian Skeletal Muscle
doi: 10.3390/biom16010135
Figure Lengend Snippet: Effect of capsaicin on the kinetics and the current–voltage relationship of calcium currents, and summary of effects of test compounds on calcium currents. ( A ) Effect of capsaicin on L-type Ca 2+ current kinetics in rat myotubes. Means and the S.E.M. of the time to reach peak amplitude and inactivation time constant (τ) are shown before (control, white bars) and five min following 30 μM capsaicin (gray bars) application ( p < 0.05; ANOVA). ( B ) Current–voltage relationship of Ca 2+ currents in the absence (filled circles) and presence (open circles) of 30 μM capsaicin is presented. The 500 ms depolarizing voltage steps from a holding potential of −80 mV to command potentials between −50 and +70 mV were used to evoke currents. The data points are the mean and the S.E.M. values from five cells. ( C ) The effects of 30 µM capsaicin on Ca 2+ currents activated by depolarizations to +10 mV from holding potentials of −80 mV or −50 mV ( n = 5 cells). ( D ) The effects of capsaicin and TRPV1 antagonists on the maximal amplitudes of L-type Ca 2+ currents in rat myotubes. CAP: capsaicin; CPZ: capsazepine; SB: SB-366791; VAN: vanillin. * denotes statistical significance at the level of p < 0.05 when compared to the controls.
Article Snippet: Capsaicin, other capsaicin analogs, vanillyl and its derivatives, SB-366791, and all other reagents were obtained from Sigma (St. Louis, MO, USA), while
Techniques: Control