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biotinylated val ala asp ome fluoromethyl ketone  (Valiant Co Ltd)
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Valiant Co Ltd biotinylated val ala asp ome fluoromethyl ketone
Increased activation of ERK and NF-κB in p43FLIP T cells. Purified T cells from p43FLIP mice or normal littermate control (NLC) mice were stimulated with <t>biotinylated</t> anti-CD3/anti-CD28 antibody and cross-linked with streptavidin for the times indicated. The proteasome blocker MG132 was added to some samples to prevent degradation of phospho-IκBα. Lysates were then analyzed using Bio-Plex for ERK phosphorylation (A) and IκBα phosphorylation (B) as a ratio of the phosphorylated form of each molecule to the total amount of the same molecule. Shown are the means ± S.D. of three experiments expressed as -fold increase in the phospho-ERK/total ERK or phospho-IκBα/total IκBα ratios over unstimulated cells (time 0). Statistical analysis was done over the complete time period using repeated measures ANOVA (phospho-ERK, p < 0.0001; phospho-IκBα, p = 0.0038). Independently, lysates were also analyzed by immunoblotting for phospho-ERK, total ERK, phospho-MEK, and total MEK (C) and phospho-IκBα and total IκBα (D). The findings obtained by immunoblotting were consistent in two experiments using p43FLIP T cells, as well as in two experiments using p43FLIP Jurkat T cells (not shown). PMA, phorbol 12-myristate 13-acetate.
Biotinylated Val Ala Asp Ome Fluoromethyl Ketone, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/03fk01401/Biotin-Val-Ala-Asp(Ome)-fluoromethylketone/pmc03887185-224-15-19
Average 90 stars, based on 1 article reviews
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Increased activation of ERK and NF-κB in p43FLIP T cells. Purified T cells from p43FLIP mice or normal littermate control (NLC) mice were stimulated with biotinylated anti-CD3/anti-CD28 antibody and cross-linked with streptavidin for the times indicated. The proteasome blocker MG132 was added to some samples to prevent degradation of phospho-IκBα. Lysates were then analyzed using Bio-Plex for ERK phosphorylation (A) and IκBα phosphorylation (B) as a ratio of the phosphorylated form of each molecule to the total amount of the same molecule. Shown are the means ± S.D. of three experiments expressed as -fold increase in the phospho-ERK/total ERK or phospho-IκBα/total IκBα ratios over unstimulated cells (time 0). Statistical analysis was done over the complete time period using repeated measures ANOVA (phospho-ERK, p < 0.0001; phospho-IκBα, p = 0.0038). Independently, lysates were also analyzed by immunoblotting for phospho-ERK, total ERK, phospho-MEK, and total MEK (C) and phospho-IκBα and total IκBα (D). The findings obtained by immunoblotting were consistent in two experiments using p43FLIP T cells, as well as in two experiments using p43FLIP Jurkat T cells (not shown). PMA, phorbol 12-myristate 13-acetate.

Journal: The Journal of Biological Chemistry

Article Title: The c-FLIP L Cleavage Product p43FLIP Promotes Activation of Extracellular Signal-regulated Kinase (ERK), Nuclear Factor κB (NF-κB), and Caspase-8 and T Cell Survival *

doi: 10.1074/jbc.M113.506428

Figure Lengend Snippet: Increased activation of ERK and NF-κB in p43FLIP T cells. Purified T cells from p43FLIP mice or normal littermate control (NLC) mice were stimulated with biotinylated anti-CD3/anti-CD28 antibody and cross-linked with streptavidin for the times indicated. The proteasome blocker MG132 was added to some samples to prevent degradation of phospho-IκBα. Lysates were then analyzed using Bio-Plex for ERK phosphorylation (A) and IκBα phosphorylation (B) as a ratio of the phosphorylated form of each molecule to the total amount of the same molecule. Shown are the means ± S.D. of three experiments expressed as -fold increase in the phospho-ERK/total ERK or phospho-IκBα/total IκBα ratios over unstimulated cells (time 0). Statistical analysis was done over the complete time period using repeated measures ANOVA (phospho-ERK, p < 0.0001; phospho-IκBα, p = 0.0038). Independently, lysates were also analyzed by immunoblotting for phospho-ERK, total ERK, phospho-MEK, and total MEK (C) and phospho-IκBα and total IκBα (D). The findings obtained by immunoblotting were consistent in two experiments using p43FLIP T cells, as well as in two experiments using p43FLIP Jurkat T cells (not shown). PMA, phorbol 12-myristate 13-acetate.

Article Snippet: Viable day 4 T cell lymphoblasts were disrupted using lysis buffer containing 20 μ m biotinylated Val-Ala-Asp(OMe)-fluoromethyl ketone (biotin-VAD; MP Biomedicals).

Techniques: Activation Assay, Purification, Western Blot