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    Valiant ac devd afc
    Caspase activity in midgut and remaining carcass following injection of dsRNA. Rexville D mosquitoes were injected with dsRNA ( Aeiap1 , Aedronc , or cat ) or PBS or mock injected. Midguts were dissected at 1, 2, or 3 days postinjection, and pooled tissues from 10 individuals were homogenized in lysis buffer. Caspase activity was determined in midguts (A) and remaining carcass (B) using the effector caspase substrate <t>Ac-DEVD-AFC.</t> Data are means ± standard errors of the means from three independent experiments, and statistical significance (****, P
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    Caspase activity in midgut and remaining carcass following injection of dsRNA. Rexville D mosquitoes were injected with dsRNA ( Aeiap1 , Aedronc , or cat ) or PBS or mock injected. Midguts were dissected at 1, 2, or 3 days postinjection, and pooled tissues from 10 individuals were homogenized in lysis buffer. Caspase activity was determined in midguts (A) and remaining carcass (B) using the effector caspase substrate Ac-DEVD-AFC. Data are means ± standard errors of the means from three independent experiments, and statistical significance (****, P

    Journal: Journal of Virology

    Article Title: Effects of Manipulating Apoptosis on Sindbis Virus Infection of Aedes aegypti Mosquitoes

    doi: 10.1128/JVI.00125-12

    Figure Lengend Snippet: Caspase activity in midgut and remaining carcass following injection of dsRNA. Rexville D mosquitoes were injected with dsRNA ( Aeiap1 , Aedronc , or cat ) or PBS or mock injected. Midguts were dissected at 1, 2, or 3 days postinjection, and pooled tissues from 10 individuals were homogenized in lysis buffer. Caspase activity was determined in midguts (A) and remaining carcass (B) using the effector caspase substrate Ac-DEVD-AFC. Data are means ± standard errors of the means from three independent experiments, and statistical significance (****, P

    Article Snippet: Protein concentration was determined by absorbance at 280 nm, and 50 μg of protein was mixed in 100 μl reaction buffer (100 mM HEPES [pH 7.4] containing 2 mM DTT, 0.1% 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate [CHAPS], 1% sucrose) with 200 μM Ac-DEVD-AFC (MP Biomedical), an effector caspase substrate, and incubated for 15 min at 37°C.

    Techniques: Activity Assay, Injection, Lysis