Journal: Nature Communications

Article Title: Lack of intestinal Mucin-1 impairs intestinal epithelial barrier and promotes metabolic dysfunction-associated steatotic liver disease in male mice

doi: 10.1038/s41467-025-67034-7

Figure Lengend Snippet: a Experimental schematic of Muc1 Flox/Flox and Muc1 EKO mice after 16 weeks of ND or HFD diet. The diagram of the mouse was created in BioRender. Li, Z. (2025) https://BioRender.com/e2kqno5 . Mice were housed at room temperature (RT, 21 °C) or thermoneutrality (TN, 29 °C). CLAMS, Comprehensive Laboratory Animal Monitoring System. Villin-Cre (Villin promoter-driven Cre recombinase), Muc1 Flox/Flox (Muc1 with loxP sites flanking the gene), Muc1 EKO (Epithelial-Knockout of Muc1), ND (Normal Diet), HFD (High-Fat Diet). b Typical liver H&E staining, oil red O staining, Sirius red staining, and Immunohistochemical staining of F4/80. Scale bars: 25 μm. c Body weight, liver weight. d Glucose tolerance test (GTT). e Insulin tolerance test (ITT). f Quantitative analysis of Serum TG, Hepatic TG, ALT, and AST. g , h Inflammation-related ( g ) and Fibrosis-related ( h ) Gene expression in liver tissues. Data are mean ± s.e.m. n = 8 mice per group. P -values were calculated by a two-tailed unpaired t -test. Source data are provided as a Source data file.

Article Snippet: Muc1 Flox/Flox and β-Catenin Flox/Flox mice were purchased from Cyagen (S-CKO-03832, S-CKO-01559).

Techniques: Knock-Out, Staining, Immunohistochemical staining, Gene Expression, Two Tailed Test

Journal: Nature Communications

Article Title: Lack of intestinal Mucin-1 impairs intestinal epithelial barrier and promotes metabolic dysfunction-associated steatotic liver disease in male mice

doi: 10.1038/s41467-025-67034-7

Figure Lengend Snippet: a LPS Concentration were measured in the serum of Muc1 Flox/Flox and Muc1 EKO mice ( n = 8 per group). b Bacterial DNA/Whole DNA in blood was measured by RT-qPCR ( n = 8 per group). c Plasmatic 4KDa FITC Dextran Flux concentration 4 h after gavage by 750 µg/g bodyweight ( n = 8 per group). d Representative transmission electron microscopy images and Width of colonic epithelial cell zonula adherence in mice ( n = 8). Scale bars: 1 µm (left), 200 nm (right). e Fluorescent confocal images of colon tissue sections of mice and humans with MASLD and their control group. Scale bars: 40 μm. f Relative protein expression of MUC1 and β-Catenin in colon tissues of the control group and MASLD group ( n = 30 per group). g Relative protein expression of MUC1 and β-Catenin in colon tissues of mice fed with ND and HFD ( n = 6 per group). h Fluorescent confocal images of colon tissue sections of Muc1 Flox/Flox and Muc1 EKO mice. Scale bars: 40 μm. i Relative protein expression of MUC1, β-Catenin, and Phospho-β-Catenin in colon tissues of Muc1 Flox/Flox and Muc1 EKO mice ( n = 8 per group). j Western blots for the indicated proteins from HT-29 cells transfected with shCTL or shMUC1 treated with 50 μM lysosome inhibitor CQ for 24 h or 10 μM proteasome inhibitor MG132 for 6 h, or DMSO as a control. CHX (Cycloheximide), DMSO (Dimethyl sulfoxide), CQ (Chloroquine), shCTL (shRNA Control), shMUC1 (shRNA targeting MUC1). Data are means ± SEM. P -values were calculated by a two-tailed unpaired t -test. Western blotting data are representative of three independent experiments. Source data are provided as a Source data file.

Article Snippet: Muc1 Flox/Flox and β-Catenin Flox/Flox mice were purchased from Cyagen (S-CKO-03832, S-CKO-01559).

Techniques: Concentration Assay, Quantitative RT-PCR, Transmission Assay, Electron Microscopy, Control, Expressing, Western Blot, Transfection, shRNA, Two Tailed Test