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    Valiant mitomycin c
    Kinetics of Rad51 foci formation in V79B and CL-V8B cells after (A) <t>BLM</t> and (B) <t>MMC</t> exposure. V-C8 defective in Brca2 / Fancd1 (HR and Fanconi anemia pathway) represent control cell line with Rad51 foci impairment. Data represent mean values from at least three independent experiments. Error bars represent standard error. BLM, bleomycin; MMC, mitomycin C; HR, homologous recombination.
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    Kinetics of Rad51 foci formation in V79B and CL-V8B cells after (A) BLM and (B) MMC exposure. V-C8 defective in Brca2 / Fancd1 (HR and Fanconi anemia pathway) represent control cell line with Rad51 foci impairment. Data represent mean values from at least three independent experiments. Error bars represent standard error. BLM, bleomycin; MMC, mitomycin C; HR, homologous recombination.

    Journal: Molecular Medicine Reports

    Article Title: Distinct cellular phenotype linked to defective DNA interstrand crosslink repair and homologous recombination

    doi: 10.3892/mmr.2017.6781

    Figure Lengend Snippet: Kinetics of Rad51 foci formation in V79B and CL-V8B cells after (A) BLM and (B) MMC exposure. V-C8 defective in Brca2 / Fancd1 (HR and Fanconi anemia pathway) represent control cell line with Rad51 foci impairment. Data represent mean values from at least three independent experiments. Error bars represent standard error. BLM, bleomycin; MMC, mitomycin C; HR, homologous recombination.

    Article Snippet: At the indicated time points (8, 24, 48 and 56 h) after the MMC or BLM treatment, the cells were washed and fixed in 2% formaldehyde (MP Biomedicals LLC, Solon, OH, USA) for 15 min. Then, the cells were permeabilized with 0.1% Triton X-100 (MP Biomedicals LLC) and blocked for 30 min with PBS (PBS+) containing 0.15% glycine and 0.5% bovine serum albumin (Sigma-Aldrich; Merck Millipore).

    Techniques: Homologous Recombination

    Clonogenic survival of CL-V8B and the parental cell line V79B following exposure to (A) MMC, (B) CDDP, (C) UVC radiation, (D) hydrogen peroxide, (E) CPT, (F) HU, (G) BLM, (H) 6-tG, (I) poly (adenosine diphosphate-ribose) polymerase inhibitor KU0058948. Data are presented as the mean values from at least three independent experiments. Error bars represent standard error. MMC, mitomycin C; CDDP, cisplatin; CPT, camptothecin; HU, hydroxyurea; BLM, bleomycin; 6-tG, 6-tioguanine.

    Journal: Molecular Medicine Reports

    Article Title: Distinct cellular phenotype linked to defective DNA interstrand crosslink repair and homologous recombination

    doi: 10.3892/mmr.2017.6781

    Figure Lengend Snippet: Clonogenic survival of CL-V8B and the parental cell line V79B following exposure to (A) MMC, (B) CDDP, (C) UVC radiation, (D) hydrogen peroxide, (E) CPT, (F) HU, (G) BLM, (H) 6-tG, (I) poly (adenosine diphosphate-ribose) polymerase inhibitor KU0058948. Data are presented as the mean values from at least three independent experiments. Error bars represent standard error. MMC, mitomycin C; CDDP, cisplatin; CPT, camptothecin; HU, hydroxyurea; BLM, bleomycin; 6-tG, 6-tioguanine.

    Article Snippet: At the indicated time points (8, 24, 48 and 56 h) after the MMC or BLM treatment, the cells were washed and fixed in 2% formaldehyde (MP Biomedicals LLC, Solon, OH, USA) for 15 min. Then, the cells were permeabilized with 0.1% Triton X-100 (MP Biomedicals LLC) and blocked for 30 min with PBS (PBS+) containing 0.15% glycine and 0.5% bovine serum albumin (Sigma-Aldrich; Merck Millipore).

    Techniques: Cycling Probe Technology