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Valiant Co Ltd ethidium bromide efflux
Putative efflux-pump inhibitors reduce <t>ethidium</t> <t>bromide</t> efflux in serum-grown A. baumannii. Cells were grown in serum and putative efflux inhibitors ABEPI1 (cyan), analogue 10 (pink), analogue 14 (orange), analogue 16 (black) and analogue 17 (brown) were added to 2 μg/mL. DMSO (red) represents vehicle alone and full ethidium bromide efflux potential. Known efflux inhibitor PAβN (green) was added to 40 μg/mL. Cells grown in LB (blue) show little to no ethidium bromide efflux. Values are an average of three experiments performed in duplicate ± SD.
Ethidium Bromide Efflux, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Putative efflux-pump inhibitors reduce ethidium bromide efflux in serum-grown A. baumannii. Cells were grown in serum and putative efflux inhibitors ABEPI1 (cyan), analogue 10 (pink), analogue 14 (orange), analogue 16 (black) and analogue 17 (brown) were added to 2 μg/mL. DMSO (red) represents vehicle alone and full ethidium bromide efflux potential. Known efflux inhibitor PAβN (green) was added to 40 μg/mL. Cells grown in LB (blue) show little to no ethidium bromide efflux. Values are an average of three experiments performed in duplicate ± SD.

Journal: ChemMedChem

Article Title: Optimization of 4-Substituted Benzenesulfonamide Scaffold To Reverse Acinetobacter baumannii Serum-Adaptive Efflux Associated Antibiotic Tolerance

doi: 10.1002/cmdc.202000328

Figure Lengend Snippet: Putative efflux-pump inhibitors reduce ethidium bromide efflux in serum-grown A. baumannii. Cells were grown in serum and putative efflux inhibitors ABEPI1 (cyan), analogue 10 (pink), analogue 14 (orange), analogue 16 (black) and analogue 17 (brown) were added to 2 μg/mL. DMSO (red) represents vehicle alone and full ethidium bromide efflux potential. Known efflux inhibitor PAβN (green) was added to 40 μg/mL. Cells grown in LB (blue) show little to no ethidium bromide efflux. Values are an average of three experiments performed in duplicate ± SD.

Article Snippet: To determine the effect on ethidium bromide efflux, the cells were treated with 2 μg/mL compound or 40 μg/mL phenylalanine arginine β-naphthylamide (PAβN; MP Biomedicals, Irvine, CA).

Techniques: