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Restoration of cAMP levels does not induce differentiation in LanCL2 KD cells. A: Cellular cAMP levels in scramble and LanCL2 KD cells before and after the addition of MDI. B: Increase in cAMP concentrations in 3T3-L1 cells by treatment with <t>forskolin.</t> C: Differentiation of scramble and LanCL2 KD cells in the presence of increasing concentrations of forskolin. D: Quantification of Oil Red O staining of differentiated scramble and LanCL2 KD cells in C. Data represent the average of two independent experiments. E: Differentiation of scramble and LanCL2 KD cells in the presence of increasing concentrations of 8-Br-cAMP. F: Quantification of Oil Red O staining of differentiated scramble and LanCL2 KD cells in E. Data represent the average of two independent experiments. One-way-ANOVA was used to compare each data point to scramble control. * P < 0.05; ** P < 0.01.
Forskolin, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/0219066901/Forskolin/pmc06071776-153-0-3
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forskolin - by Bioz Stars, 2026-09
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Restoration of cAMP levels does not induce differentiation in LanCL2 KD cells. A: Cellular cAMP levels in scramble and LanCL2 KD cells before and after the addition of MDI. B: Increase in cAMP concentrations in 3T3-L1 cells by treatment with forskolin. C: Differentiation of scramble and LanCL2 KD cells in the presence of increasing concentrations of forskolin. D: Quantification of Oil Red O staining of differentiated scramble and LanCL2 KD cells in C. Data represent the average of two independent experiments. E: Differentiation of scramble and LanCL2 KD cells in the presence of increasing concentrations of 8-Br-cAMP. F: Quantification of Oil Red O staining of differentiated scramble and LanCL2 KD cells in E. Data represent the average of two independent experiments. One-way-ANOVA was used to compare each data point to scramble control. * P < 0.05; ** P < 0.01.

Journal: Journal of Lipid Research

Article Title: Lanthionine synthetase C-like protein 2 (LanCL2) is important for adipogenic differentiation [S]

doi: 10.1194/jlr.M085274

Figure Lengend Snippet: Restoration of cAMP levels does not induce differentiation in LanCL2 KD cells. A: Cellular cAMP levels in scramble and LanCL2 KD cells before and after the addition of MDI. B: Increase in cAMP concentrations in 3T3-L1 cells by treatment with forskolin. C: Differentiation of scramble and LanCL2 KD cells in the presence of increasing concentrations of forskolin. D: Quantification of Oil Red O staining of differentiated scramble and LanCL2 KD cells in C. Data represent the average of two independent experiments. E: Differentiation of scramble and LanCL2 KD cells in the presence of increasing concentrations of 8-Br-cAMP. F: Quantification of Oil Red O staining of differentiated scramble and LanCL2 KD cells in E. Data represent the average of two independent experiments. One-way-ANOVA was used to compare each data point to scramble control. * P < 0.05; ** P < 0.01.

Article Snippet: Forskolin was from MP Biomedicals (Solon, OH), and 8-Br-cAMP was from Tocris (Ellisville, MO).

Techniques: Staining