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Demonstrating the generalizability of RT-Exo to characterize aptamers with distinct structures binding various targets. (A) Structure of the adenosine aptamer conjugate A23T-R91–4bp. (B) RT-Exo assay data showing fluorescence over time at different concentrations of adenosine, and (C) specificity of A23T-R91–4bp as determined using the RT-Exo assay. (D) Structure of the THC aptamer conjugate THC1.2-R91–4bp. (E, F) RT-Exo assay data for THC1.2-R91–4bp (E) binding to THC and (F) specificity. (G) Structure of the cocaine aptamer conjugate MNS4.1-R91–4bp. (H, I) RT-Exo assay data for MNS4.1-R91–4bp (H) binding to cocaine and (I) specificity. THCC: (±)-11-nor-9-carboxy-Δ 9 -THC, CBN: cannabinol, THCV: tetrahydrocannabivarin, THCA: THC carboxylic acid A, CBD: cannabidiol, CBDA: cannabidiolic acid, CBGA: cannabigerolic acid, COC: cocaine, AMP: amphetamine, MTC: methcathinone, PENT: pentylone, CLO: clonazepam, CAF: caffeine, PRO: procaine, ACM: acetaminophen, IBU: ibuprofen, NIC: nicotine, BE: benzoylecgonine, MEPH: mephedrone, MDPV: methylenedioxypyrovalerone, FENT: fentanyl, METH: (+)-methamphetamine, MOR: morphine, MTP: methylphenidate, HER: heroin, PSE: pseudoephedrine, MTD: methadone, BZC: benzocaine, SCP: scopolamine, FLU: fluoxetine, SER: serotonin, DOPA: dopamine, LAC: lactose, MAN: mannitol, LIDO: lidocaine, DPH: diphenhydramine, LEV: <t>levamisole,</t> OXY: oxycodone, QUI: quinine, FUB: AB-FUBINACA, ALP: alprazolam, DIAZ: diazepam.
Levamisole Hcl, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Demonstrating the generalizability of RT-Exo to characterize aptamers with distinct structures binding various targets. (A) Structure of the adenosine aptamer conjugate A23T-R91–4bp. (B) RT-Exo assay data showing fluorescence over time at different concentrations of adenosine, and (C) specificity of A23T-R91–4bp as determined using the RT-Exo assay. (D) Structure of the THC aptamer conjugate THC1.2-R91–4bp. (E, F) RT-Exo assay data for THC1.2-R91–4bp (E) binding to THC and (F) specificity. (G) Structure of the cocaine aptamer conjugate MNS4.1-R91–4bp. (H, I) RT-Exo assay data for MNS4.1-R91–4bp (H) binding to cocaine and (I) specificity. THCC: (±)-11-nor-9-carboxy-Δ 9 -THC, CBN: cannabinol, THCV: tetrahydrocannabivarin, THCA: THC carboxylic acid A, CBD: cannabidiol, CBDA: cannabidiolic acid, CBGA: cannabigerolic acid, COC: cocaine, AMP: amphetamine, MTC: methcathinone, PENT: pentylone, CLO: clonazepam, CAF: caffeine, PRO: procaine, ACM: acetaminophen, IBU: ibuprofen, NIC: nicotine, BE: benzoylecgonine, MEPH: mephedrone, MDPV: methylenedioxypyrovalerone, FENT: fentanyl, METH: (+)-methamphetamine, MOR: morphine, MTP: methylphenidate, HER: heroin, PSE: pseudoephedrine, MTD: methadone, BZC: benzocaine, SCP: scopolamine, FLU: fluoxetine, SER: serotonin, DOPA: dopamine, LAC: lactose, MAN: mannitol, LIDO: lidocaine, DPH: diphenhydramine, LEV: levamisole, OXY: oxycodone, QUI: quinine, FUB: AB-FUBINACA, ALP: alprazolam, DIAZ: diazepam.

Journal: Journal of the American Chemical Society

Article Title: High-Throughput Aptamer Characterization via Real-Time Nuclease Digestion

doi: 10.1021/jacs.5c17561

Figure Lengend Snippet: Demonstrating the generalizability of RT-Exo to characterize aptamers with distinct structures binding various targets. (A) Structure of the adenosine aptamer conjugate A23T-R91–4bp. (B) RT-Exo assay data showing fluorescence over time at different concentrations of adenosine, and (C) specificity of A23T-R91–4bp as determined using the RT-Exo assay. (D) Structure of the THC aptamer conjugate THC1.2-R91–4bp. (E, F) RT-Exo assay data for THC1.2-R91–4bp (E) binding to THC and (F) specificity. (G) Structure of the cocaine aptamer conjugate MNS4.1-R91–4bp. (H, I) RT-Exo assay data for MNS4.1-R91–4bp (H) binding to cocaine and (I) specificity. THCC: (±)-11-nor-9-carboxy-Δ 9 -THC, CBN: cannabinol, THCV: tetrahydrocannabivarin, THCA: THC carboxylic acid A, CBD: cannabidiol, CBDA: cannabidiolic acid, CBGA: cannabigerolic acid, COC: cocaine, AMP: amphetamine, MTC: methcathinone, PENT: pentylone, CLO: clonazepam, CAF: caffeine, PRO: procaine, ACM: acetaminophen, IBU: ibuprofen, NIC: nicotine, BE: benzoylecgonine, MEPH: mephedrone, MDPV: methylenedioxypyrovalerone, FENT: fentanyl, METH: (+)-methamphetamine, MOR: morphine, MTP: methylphenidate, HER: heroin, PSE: pseudoephedrine, MTD: methadone, BZC: benzocaine, SCP: scopolamine, FLU: fluoxetine, SER: serotonin, DOPA: dopamine, LAC: lactose, MAN: mannitol, LIDO: lidocaine, DPH: diphenhydramine, LEV: levamisole, OXY: oxycodone, QUI: quinine, FUB: AB-FUBINACA, ALP: alprazolam, DIAZ: diazepam.

Article Snippet: Levamisole HCl was purchased from MP Biomedicals.

Techniques: Binding Assay, Fluorescence