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    Valiant diclofenac
    Elution of flavone B from colon. HPLC chromatographs of a blank colon; b colon spiked with internal standard <t>(diclofenac</t> 25 µg/mL); c colon spiked with flavone B (100 µg/mL) and internal standard
    Diclofenac, supplied by Valiant, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    diclofenac - by Bioz Stars, 2021-04
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    Elution of flavone B from colon. HPLC chromatographs of a blank colon; b colon spiked with internal standard (diclofenac 25 µg/mL); c colon spiked with flavone B (100 µg/mL) and internal standard

    Journal: BMC Research Notes

    Article Title: Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatography

    doi: 10.1186/s13104-016-2358-y

    Figure Lengend Snippet: Elution of flavone B from colon. HPLC chromatographs of a blank colon; b colon spiked with internal standard (diclofenac 25 µg/mL); c colon spiked with flavone B (100 µg/mL) and internal standard

    Article Snippet: Stock solutions of 100 µg/mL of flavone B, prepared as described previously [ ], and 25 µg/mL diclofenac (MP Biomedicals, LLC; Solon, OH) were prepared with acetonitrile/water/acetic acid/trimethylamine (70:30:0.2:0.05).

    Techniques: High Performance Liquid Chromatography

    Principal component analysis scores (PC1, PC2, and PC3) for protein pattern assessment of control and treated eleutheroembryos with (a) Rotenone, (b) DNOC, and (c) Diclofenac. Control groups are indicated with c. Three independent replicates were performed for all treatment groups (EC 10 treatment with either Rotenone, DNOC, or Diclofenac).

    Journal: International Journal of Proteomics

    Article Title: Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

    doi: 10.1155/2010/630134

    Figure Lengend Snippet: Principal component analysis scores (PC1, PC2, and PC3) for protein pattern assessment of control and treated eleutheroembryos with (a) Rotenone, (b) DNOC, and (c) Diclofenac. Control groups are indicated with c. Three independent replicates were performed for all treatment groups (EC 10 treatment with either Rotenone, DNOC, or Diclofenac).

    Article Snippet: All substances were of analytical grade and purchased from Riedel de Haen (Rotenone and DNOC) and MP Biomedicals (Diclofenac), respectively.

    Techniques:

    Summary of results from spot-to-spot analysis of proteomics experiments with Diclofenac (Dic), Rotenone(R), and DNOC (D). For all tested concentrations the percentages of protein spots, related to all detected protein spots in the 2-DE experiment, are depicted that show significant ( P

    Journal: International Journal of Proteomics

    Article Title: Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

    doi: 10.1155/2010/630134

    Figure Lengend Snippet: Summary of results from spot-to-spot analysis of proteomics experiments with Diclofenac (Dic), Rotenone(R), and DNOC (D). For all tested concentrations the percentages of protein spots, related to all detected protein spots in the 2-DE experiment, are depicted that show significant ( P

    Article Snippet: All substances were of analytical grade and purchased from Riedel de Haen (Rotenone and DNOC) and MP Biomedicals (Diclofenac), respectively.

    Techniques:

    Microscopically visible lethal effects of Rotenone, DNOC and Diclofenac to eleuthero-embryos studied after 48 h exposure. Concentration-effect relationships based on a logistic model are shown. Parameter estimates were as follows: Rotenone: EC 50 [ μ M] = 0.068 ± 0.00, p = 8.750 ± 2.25, DNOC: EC 50 [ μ M] = 3.007 ± 0.05, p = 12.311 ± 1.80, Diclofenac: EC 50 [ μ M] = 23.076 ± 1.08, p = 4.746 ± 1.65.

    Journal: International Journal of Proteomics

    Article Title: Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

    doi: 10.1155/2010/630134

    Figure Lengend Snippet: Microscopically visible lethal effects of Rotenone, DNOC and Diclofenac to eleuthero-embryos studied after 48 h exposure. Concentration-effect relationships based on a logistic model are shown. Parameter estimates were as follows: Rotenone: EC 50 [ μ M] = 0.068 ± 0.00, p = 8.750 ± 2.25, DNOC: EC 50 [ μ M] = 3.007 ± 0.05, p = 12.311 ± 1.80, Diclofenac: EC 50 [ μ M] = 23.076 ± 1.08, p = 4.746 ± 1.65.

    Article Snippet: All substances were of analytical grade and purchased from Riedel de Haen (Rotenone and DNOC) and MP Biomedicals (Diclofenac), respectively.

    Techniques: Concentration Assay

    2-DE gels from proteomics experiments: (a) control conditions, (b) EC 10 treatment with Rotenone, (c), EC 10 treatment with DNOC and (d) EC 10 treatment with Diclofenac. All protein samples were separated under the same 2-DE conditions: Immobiline strips pH 4–7 in the first and 14% PAA gels in the second dimension.

    Journal: International Journal of Proteomics

    Article Title: Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

    doi: 10.1155/2010/630134

    Figure Lengend Snippet: 2-DE gels from proteomics experiments: (a) control conditions, (b) EC 10 treatment with Rotenone, (c), EC 10 treatment with DNOC and (d) EC 10 treatment with Diclofenac. All protein samples were separated under the same 2-DE conditions: Immobiline strips pH 4–7 in the first and 14% PAA gels in the second dimension.

    Article Snippet: All substances were of analytical grade and purchased from Riedel de Haen (Rotenone and DNOC) and MP Biomedicals (Diclofenac), respectively.

    Techniques:

    Comparison of results from toxicoproteomics experiments with Rotenone, DNOC, and Diclofenac exposure. (a) PCA scores when all samples are included in PCA analysis. (b) Venn-Diagramm based on results from substance-specific univariate spot-to-spot analysis. Most of the protein spots changed specifically. 9 proteins concurrently changed after Rotenone, DNOC, and Diclofenac treatment. Moreover, 13 proteins simultaneously changed after Rotenone and DNOC treatment, and 14 proteins showed changed expression levels at exposure against Diclofenac or Rotenone. Only one protein was found to be changed in the larval protein samples after DNOC as well as Diclofenac treatment. (c) 2-DE gel from eleutheroembryonal protein samples. All proteins that significantly changed in expression in at least two toxicoproteomics experiments (treatment with either Rotenone, DNOC, or Diclofenac) are labelled: Protein labels of protein spots simultaneously altered in Rotenone, DNOC, and Diclofenac experiments are framed (□), in Rotenone and DNOC experiments are underlined (—), in Rotenone and Diclofenac experiments are dashed (—), and in DNOC, and Diclofenac experiments are labelled by name only.

    Journal: International Journal of Proteomics

    Article Title: Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

    doi: 10.1155/2010/630134

    Figure Lengend Snippet: Comparison of results from toxicoproteomics experiments with Rotenone, DNOC, and Diclofenac exposure. (a) PCA scores when all samples are included in PCA analysis. (b) Venn-Diagramm based on results from substance-specific univariate spot-to-spot analysis. Most of the protein spots changed specifically. 9 proteins concurrently changed after Rotenone, DNOC, and Diclofenac treatment. Moreover, 13 proteins simultaneously changed after Rotenone and DNOC treatment, and 14 proteins showed changed expression levels at exposure against Diclofenac or Rotenone. Only one protein was found to be changed in the larval protein samples after DNOC as well as Diclofenac treatment. (c) 2-DE gel from eleutheroembryonal protein samples. All proteins that significantly changed in expression in at least two toxicoproteomics experiments (treatment with either Rotenone, DNOC, or Diclofenac) are labelled: Protein labels of protein spots simultaneously altered in Rotenone, DNOC, and Diclofenac experiments are framed (□), in Rotenone and DNOC experiments are underlined (—), in Rotenone and Diclofenac experiments are dashed (—), and in DNOC, and Diclofenac experiments are labelled by name only.

    Article Snippet: All substances were of analytical grade and purchased from Riedel de Haen (Rotenone and DNOC) and MP Biomedicals (Diclofenac), respectively.

    Techniques: Expressing