01341 Search Results


zbp1  (Cyagen Biosciences)
92
Cyagen Biosciences zbp1
Figure 5. Palmitic acid upregulates hepatocyte <t>ZBP1</t> expression level through JNK pathway to exacerbate I/R injury of steatotic livers. (A and B) ZBP1 mRNA levels in donor livers (n = 6) or mouse I/R model (n = 4 for sham and n = 8 for I/R) were quantified. (C and D) mRNA and protein levels of ZBP1 after different concentration of PA in normal PMH for 48 hours (n = 5). (E and F) mRNA and protein levels of ZBP1 after stimulation of 0.4 mM PA and 10μM SP600125 in normal PHH for 24 hours (n = 5). (G and H) mRNA and protein levels of ZBP1 after stimulation of 0.4 mM PA for 24 hours in c-Jun knockdown normal PHH (n = 5). (I) ZBP1 promoter mutation schema and luciferase activity after c-Jun overexpression (n = 6). (J) Binding of c-Jun to ZBP1 promoter after stimulation of PA and SP600125 treatment or c-Jun knockdown in normal PHH (n = 6). (K–M) Zbp1+/+ or Zbp1–/– PMH (n = 3) were pretreated with 0.2 mM PA for 24 hours and subjected to 10 h hypoxia. Cell death (K) was analyzed after 24 hours reoxygenation and apoptosis (L) and mRNA levels of cyto- kines (M) was analyzed after 3 hours reoxygenation. All data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. 2-way ANOVA, post hoc Bonferroni’s test (A, B, I, J, and M). 1-way ANOVA, post hoc Dunnett’s test (C, E, and G). Unpaired, 2-tailed Student’s t test (K).
Zbp1, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/01341/Casp8/10__1172_slash_jci180451-228-6-12
Average 92 stars, based on 1 article reviews
zbp1 - by Bioz Stars, 2026-09
92/100 stars
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boc cys mob  (Chem Impex International)
95
Chem Impex International boc cys mob
Figure 5. Palmitic acid upregulates hepatocyte <t>ZBP1</t> expression level through JNK pathway to exacerbate I/R injury of steatotic livers. (A and B) ZBP1 mRNA levels in donor livers (n = 6) or mouse I/R model (n = 4 for sham and n = 8 for I/R) were quantified. (C and D) mRNA and protein levels of ZBP1 after different concentration of PA in normal PMH for 48 hours (n = 5). (E and F) mRNA and protein levels of ZBP1 after stimulation of 0.4 mM PA and 10μM SP600125 in normal PHH for 24 hours (n = 5). (G and H) mRNA and protein levels of ZBP1 after stimulation of 0.4 mM PA for 24 hours in c-Jun knockdown normal PHH (n = 5). (I) ZBP1 promoter mutation schema and luciferase activity after c-Jun overexpression (n = 6). (J) Binding of c-Jun to ZBP1 promoter after stimulation of PA and SP600125 treatment or c-Jun knockdown in normal PHH (n = 6). (K–M) Zbp1+/+ or Zbp1–/– PMH (n = 3) were pretreated with 0.2 mM PA for 24 hours and subjected to 10 h hypoxia. Cell death (K) was analyzed after 24 hours reoxygenation and apoptosis (L) and mRNA levels of cyto- kines (M) was analyzed after 3 hours reoxygenation. All data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. 2-way ANOVA, post hoc Bonferroni’s test (A, B, I, J, and M). 1-way ANOVA, post hoc Dunnett’s test (C, E, and G). Unpaired, 2-tailed Student’s t test (K).
Boc Cys Mob, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/01341/Boc-L-cysteine/pm16706526__ol0606740si20060317_033636-27-0-4
Average 95 stars, based on 1 article reviews
boc cys mob - by Bioz Stars, 2026-09
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blue dextran 2000  (Kyfora Bio)
92
Kyfora Bio blue dextran 2000
Figure 5. Palmitic acid upregulates hepatocyte <t>ZBP1</t> expression level through JNK pathway to exacerbate I/R injury of steatotic livers. (A and B) ZBP1 mRNA levels in donor livers (n = 6) or mouse I/R model (n = 4 for sham and n = 8 for I/R) were quantified. (C and D) mRNA and protein levels of ZBP1 after different concentration of PA in normal PMH for 48 hours (n = 5). (E and F) mRNA and protein levels of ZBP1 after stimulation of 0.4 mM PA and 10μM SP600125 in normal PHH for 24 hours (n = 5). (G and H) mRNA and protein levels of ZBP1 after stimulation of 0.4 mM PA for 24 hours in c-Jun knockdown normal PHH (n = 5). (I) ZBP1 promoter mutation schema and luciferase activity after c-Jun overexpression (n = 6). (J) Binding of c-Jun to ZBP1 promoter after stimulation of PA and SP600125 treatment or c-Jun knockdown in normal PHH (n = 6). (K–M) Zbp1+/+ or Zbp1–/– PMH (n = 3) were pretreated with 0.2 mM PA for 24 hours and subjected to 10 h hypoxia. Cell death (K) was analyzed after 24 hours reoxygenation and apoptosis (L) and mRNA levels of cyto- kines (M) was analyzed after 3 hours reoxygenation. All data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. 2-way ANOVA, post hoc Bonferroni’s test (A, B, I, J, and M). 1-way ANOVA, post hoc Dunnett’s test (C, E, and G). Unpaired, 2-tailed Student’s t test (K).
Blue Dextran 2000, supplied by Kyfora Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/01341/Dextran/10__1016_slash_s0021___9258_ascii40_18_ascii41_50398___7-49-10-22
Average 92 stars, based on 1 article reviews
blue dextran 2000 - by Bioz Stars, 2026-09
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3 amino 2 naphthoic acid  (Chem Impex International)
95
Chem Impex International 3 amino 2 naphthoic acid
Figure 5. Palmitic acid upregulates hepatocyte <t>ZBP1</t> expression level through JNK pathway to exacerbate I/R injury of steatotic livers. (A and B) ZBP1 mRNA levels in donor livers (n = 6) or mouse I/R model (n = 4 for sham and n = 8 for I/R) were quantified. (C and D) mRNA and protein levels of ZBP1 after different concentration of PA in normal PMH for 48 hours (n = 5). (E and F) mRNA and protein levels of ZBP1 after stimulation of 0.4 mM PA and 10μM SP600125 in normal PHH for 24 hours (n = 5). (G and H) mRNA and protein levels of ZBP1 after stimulation of 0.4 mM PA for 24 hours in c-Jun knockdown normal PHH (n = 5). (I) ZBP1 promoter mutation schema and luciferase activity after c-Jun overexpression (n = 6). (J) Binding of c-Jun to ZBP1 promoter after stimulation of PA and SP600125 treatment or c-Jun knockdown in normal PHH (n = 6). (K–M) Zbp1+/+ or Zbp1–/– PMH (n = 3) were pretreated with 0.2 mM PA for 24 hours and subjected to 10 h hypoxia. Cell death (K) was analyzed after 24 hours reoxygenation and apoptosis (L) and mRNA levels of cyto- kines (M) was analyzed after 3 hours reoxygenation. All data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. 2-way ANOVA, post hoc Bonferroni’s test (A, B, I, J, and M). 1-way ANOVA, post hoc Dunnett’s test (C, E, and G). Unpaired, 2-tailed Student’s t test (K).
3 Amino 2 Naphthoic Acid, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/01341/3-Amino-2-naphthoic+acid/pm31656961-59-0-6
Average 95 stars, based on 1 article reviews
3 amino 2 naphthoic acid - by Bioz Stars, 2026-09
95/100 stars
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Image Search Results


Figure 5. Palmitic acid upregulates hepatocyte ZBP1 expression level through JNK pathway to exacerbate I/R injury of steatotic livers. (A and B) ZBP1 mRNA levels in donor livers (n = 6) or mouse I/R model (n = 4 for sham and n = 8 for I/R) were quantified. (C and D) mRNA and protein levels of ZBP1 after different concentration of PA in normal PMH for 48 hours (n = 5). (E and F) mRNA and protein levels of ZBP1 after stimulation of 0.4 mM PA and 10μM SP600125 in normal PHH for 24 hours (n = 5). (G and H) mRNA and protein levels of ZBP1 after stimulation of 0.4 mM PA for 24 hours in c-Jun knockdown normal PHH (n = 5). (I) ZBP1 promoter mutation schema and luciferase activity after c-Jun overexpression (n = 6). (J) Binding of c-Jun to ZBP1 promoter after stimulation of PA and SP600125 treatment or c-Jun knockdown in normal PHH (n = 6). (K–M) Zbp1+/+ or Zbp1–/– PMH (n = 3) were pretreated with 0.2 mM PA for 24 hours and subjected to 10 h hypoxia. Cell death (K) was analyzed after 24 hours reoxygenation and apoptosis (L) and mRNA levels of cyto- kines (M) was analyzed after 3 hours reoxygenation. All data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. 2-way ANOVA, post hoc Bonferroni’s test (A, B, I, J, and M). 1-way ANOVA, post hoc Dunnett’s test (C, E, and G). Unpaired, 2-tailed Student’s t test (K).

Journal: Journal of Clinical Investigation

Article Title: ZBP1-mediated apoptosis and inflammation exacerbate steatotic liver ischemia/reperfusion injury

doi: 10.1172/jci180451

Figure Lengend Snippet: Figure 5. Palmitic acid upregulates hepatocyte ZBP1 expression level through JNK pathway to exacerbate I/R injury of steatotic livers. (A and B) ZBP1 mRNA levels in donor livers (n = 6) or mouse I/R model (n = 4 for sham and n = 8 for I/R) were quantified. (C and D) mRNA and protein levels of ZBP1 after different concentration of PA in normal PMH for 48 hours (n = 5). (E and F) mRNA and protein levels of ZBP1 after stimulation of 0.4 mM PA and 10μM SP600125 in normal PHH for 24 hours (n = 5). (G and H) mRNA and protein levels of ZBP1 after stimulation of 0.4 mM PA for 24 hours in c-Jun knockdown normal PHH (n = 5). (I) ZBP1 promoter mutation schema and luciferase activity after c-Jun overexpression (n = 6). (J) Binding of c-Jun to ZBP1 promoter after stimulation of PA and SP600125 treatment or c-Jun knockdown in normal PHH (n = 6). (K–M) Zbp1+/+ or Zbp1–/– PMH (n = 3) were pretreated with 0.2 mM PA for 24 hours and subjected to 10 h hypoxia. Cell death (K) was analyzed after 24 hours reoxygenation and apoptosis (L) and mRNA levels of cyto- kines (M) was analyzed after 3 hours reoxygenation. All data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. 2-way ANOVA, post hoc Bonferroni’s test (A, B, I, J, and M). 1-way ANOVA, post hoc Dunnett’s test (C, E, and G). Unpaired, 2-tailed Student’s t test (K).

Article Snippet: Casp8fl/fl (S-CKO-01552), Mlkl–/– (S-KO-14468), Ripk3–/– (S-KO-10874), Zbp1–/– (S-KO-11088) mice were obtained from Cyagen, China.

Techniques: Expressing, Concentration Assay, Knockdown, Mutagenesis, Luciferase, Activity Assay, Over Expression, Binding Assay

Figure 6. Z-NA sensing is not required for ZBP1-induced liver I/R injury. (A–F) Different truncation mutants of Zbp1 (A) were packaged into AAV8 and injected to ND-fed mice and I/R operation was conducted 1 month later (n = 8). Cell death and ZBP1-RIPK1 interaction analysis (B), serum ALT/AST detec- tion (C), H&E staining (D), TUNEL staining (E), and detection of serum levels of the proinflammatory cytokines (F) were performed. All data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. 1-way ANOVA, post hoc Dunnett’s test. Scale bars: 200 μm.

Journal: Journal of Clinical Investigation

Article Title: ZBP1-mediated apoptosis and inflammation exacerbate steatotic liver ischemia/reperfusion injury

doi: 10.1172/jci180451

Figure Lengend Snippet: Figure 6. Z-NA sensing is not required for ZBP1-induced liver I/R injury. (A–F) Different truncation mutants of Zbp1 (A) were packaged into AAV8 and injected to ND-fed mice and I/R operation was conducted 1 month later (n = 8). Cell death and ZBP1-RIPK1 interaction analysis (B), serum ALT/AST detec- tion (C), H&E staining (D), TUNEL staining (E), and detection of serum levels of the proinflammatory cytokines (F) were performed. All data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. 1-way ANOVA, post hoc Dunnett’s test. Scale bars: 200 μm.

Article Snippet: Casp8fl/fl (S-CKO-01552), Mlkl–/– (S-KO-14468), Ripk3–/– (S-KO-10874), Zbp1–/– (S-KO-11088) mice were obtained from Cyagen, China.

Techniques: Injection, Staining, TUNEL Assay

Figure 7. ROS triggers ZBP1 aggregation and activation in liver I/R injury. (A) Flag immunostaining was performed in I/R-challenged ND-fed mouse livers overexpressing Zbp1-Flag (n = 8). (B) ZBP1 aggregation was detected with immunoblot under nonreducing or reducing conditions in I/R-challenged mouse livers or transplantation-challenged donor livers (n = 3). (C) hZBP1-GFP plasmids were transfected into HEK293T cells. After 1 mM H2O2 treatment for 4 hours or H/R challenge after pretreatment with 10 mM NAC, ZBP1 oligomers were detected from green fluorescence (n = 8). (D–H) After AAV8-mediated Zbp1-Flag overexpression in ND-fed mice and pretreatment with NAC, I/R operation was performed (n = 8). Flag immunostaining (D), ZBP1 aggregation and ZBP1-RIPK1 interaction (E), serum ALT/AST levels (F), H&E and TUNEL staining (G), and detection of serum levels of the proinflammatory cytokines (H) were detected. All data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. Unpaired, 2-tailed Student’s t test (A and D). 2-way ANOVA, post hoc Bonferroni’s test (C, G, and H). Scale bars (A, C, and D): 10 μm, (G) 200 μm.

Journal: Journal of Clinical Investigation

Article Title: ZBP1-mediated apoptosis and inflammation exacerbate steatotic liver ischemia/reperfusion injury

doi: 10.1172/jci180451

Figure Lengend Snippet: Figure 7. ROS triggers ZBP1 aggregation and activation in liver I/R injury. (A) Flag immunostaining was performed in I/R-challenged ND-fed mouse livers overexpressing Zbp1-Flag (n = 8). (B) ZBP1 aggregation was detected with immunoblot under nonreducing or reducing conditions in I/R-challenged mouse livers or transplantation-challenged donor livers (n = 3). (C) hZBP1-GFP plasmids were transfected into HEK293T cells. After 1 mM H2O2 treatment for 4 hours or H/R challenge after pretreatment with 10 mM NAC, ZBP1 oligomers were detected from green fluorescence (n = 8). (D–H) After AAV8-mediated Zbp1-Flag overexpression in ND-fed mice and pretreatment with NAC, I/R operation was performed (n = 8). Flag immunostaining (D), ZBP1 aggregation and ZBP1-RIPK1 interaction (E), serum ALT/AST levels (F), H&E and TUNEL staining (G), and detection of serum levels of the proinflammatory cytokines (H) were detected. All data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. Unpaired, 2-tailed Student’s t test (A and D). 2-way ANOVA, post hoc Bonferroni’s test (C, G, and H). Scale bars (A, C, and D): 10 μm, (G) 200 μm.

Article Snippet: Casp8fl/fl (S-CKO-01552), Mlkl–/– (S-KO-14468), Ripk3–/– (S-KO-10874), Zbp1–/– (S-KO-11088) mice were obtained from Cyagen, China.

Techniques: Activation Assay, Immunostaining, Western Blot, Transplantation Assay, Transfection, Fluorescence, Over Expression, TUNEL Assay, Staining