Journal: Nature Communications

Article Title: A flexible photoacoustic retinal prosthesis

doi: 10.1038/s41467-025-67518-6

Figure Lengend Snippet: a Percentage of retinal ganglion cells (RGCs) modulated by photoacoustic (PA) stimulation per stimulation site before and after group III mGluR agonist L-AP4 application (2 retinae, n = 9 stimulation sites). b L-AP4 decreased RGC spontaneous firing rate and firing rate during PA-elicited responses in Long Evans (LE) retinae. The population firing rate of RGCs ( n = 41 cells, 2 retinae) is compared between baseline (Basal) and stimulation (Stim.), before blocker application (No blocker), following blocker application (L-AP4) and after washout (Washout) with Ringer medium. Significance of differences between fr basal and fr stim per condition, no blocker : P < 0.001; L-AP4 : P < 0.001; L-AP4 + ACET : P = 0.024; washout : P < 0.001. c L-AP4 increases response latency in LE retinae. Response latency per condition ( n = 41 cells, 2 retinae), no blocker : 49 ± 5 ms; L-AP4 : 65 ± 4 ms; washout : 42 ± 3 ms. Due to absence of responses during application of L-AP4 + ACET blockers, no latencies are reported for this group. Dashed black line: 45-ms cutoff for slow- and fast-latency responses. d Percentage of cells modulated by photoacoustic stimulation per stimulation site before and after (rs)-CPP and CNQX application in P23H retinae (2 retinae, n = 5 stimulation sites). e Glutamate blockers (rs)-CPP and CNQX abolished RGC responses to PA stimulation in P23H retinae ( n = 25 cells, 2 retinae). Significance per condition, fr basal vs fr stim : no blocker: P < 0.001; (rs)- CPP + CNQX: P = 0.141; washout: P = 0.016. Significance stimulation firing rate fr stim after blocker application and fr stim before application ( P < 0.001, two-sided Wilcoxon signed-rank), and fr stim after washout ( P < 0.001, two-sided Wilcoxon signed-rank). For each panel, groups are tested for significant differences using Friedman chi square, with post-hoc two-sided Wilcoxon signed-rank. p -values are holm-corrected (***: P < 0.001, **: P < 0.05). Box plots ( a – e ) central line: median, lower and upper bounds: first (Q1) and third quartiles (Q3), whiskers extend to the most extreme data points within 1.5 times the interquartile range from Q1 and Q3. Dashed line: mean.

Article Snippet: Kainate antagonist ( S )-1-(2-Amino-2-carboxyethyl)-3-(2-carboxy-5-phenylthiophene-3-yl-methyl)-5-methylpyrimidine-2,4-dione (ACET, 1 μM, Tocris Bioscience) was used to block remaining PA responses after L-AP4 application.

Techniques:

Journal: Human reproduction (Oxford, England)

Article Title: Interactions between galectin-3 and integrinbeta3 in regulating endometrial cell proliferation and adhesion.

doi: 10.1093/humrep/dep250

Figure Lengend Snippet: Figure 4 Western blots and flow cytometry analysis of integrins expression on gal-3 siRNA transfected cells. (A) The expression of integrinb3 was significantly increased in gal-3 silenced cells (S) compared with negative transfected (N) or mock group cells (M) (*: P , 0.05), and the change of expression of integrinb1 was not significant. (B) Flow cytometry results for integrinb1/3 expression. Isotype: cells incubated with fluorescein-conjugated IgG1 isotype and Cy5 conjugated IgG2a isotype; Test: gal-3 silenced cells; Negative: negative siRNA transfected cells; Mock: cells incubated only with lipofectamine 2000. (C) Bar graph for the flow cytometry analysis of integrinb1/3 expression. Test: gal-3 silenced cells; Negative: negative siRNA transfected cells; Mock: cells incubated only with lipofectamine 2000 (*: P , 0.05). Experiments were performed in duplicate and repeated three times. Statistical analyses were performed using One-Way ANOVA and the LSD was applied for post hoc testing using SPSS software 15.0 for windows with P , 0.05 being considered significant.

Article Snippet: Then cells were incubated with fluorescein-conjugated mouse anti-human integrinb1 monoclonal antibody (MAB1951F-100, ow nloaded from https://academ ic.oup.com /hum rep/article/24/11/2879/625632 by guest on 20 July 2024 Millipore, 1:100) or with fluorescein-conjugated mouse IgG1, K isotype (eBioscience, 1:100) as a control antibody, or with Cy5 Conjugated Mouse monoclonal anti-human integrinb3 antibody (MAB1957S-100, Millipore, 1:100) or with Cy5 Conjugated Mouse IgG2a isotype (SouthernBiotech, 1:100) as a control antibody, at room temperature for 30 min.

Techniques: Western Blot, Cytometry, Expressing, Transfection, Flow Cytometry, Incubation, Software

Journal: Journal of Virology

Article Title: Transferrin Receptor Protein 1 Is an Entry Factor for Rabies Virus

doi: 10.1128/jvi.01612-22

Figure Lengend Snippet: An antibody against TfR1 blocks RABV infection. HEK293 cells (A), N2a cells (B), or mPN cells (E) were treated with TfR1 antibody at different concentrations or IgG2a (20 μg/mL) for 1 h at 4°C and then infected with ERA-EGFP. The cell nuclei were stained (blue). The percentages of infected cells and representative images are shown. HEK293 cells (C), N2a cells (D), or mPN cells (F) were treated with TfR1 antibody or IgG2a (20 μg/mL) for 48 h. Cell viability was determined by using a Cell Titer Glo kit. The data shown are means ± the SD of three independent experiments. The two-tailed unpaired Student t test was used for the statistical analysis. ns, not significant; **, P < 0.01.

Article Snippet: The media were removed from the well, and the cells were treated with the indicated concentrations of TfR1 antibody (BD Pharmingen, 555534), or isotype antibody IgG2a (20 μg/mL; Southern Biotech, 0103-01) for 1 h on ice.

Techniques: Infection, Staining, Two Tailed Test