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Image Search Results
Journal: Cells
Article Title: Subarachnoid Hemorrhage Depletes Calcitonin Gene-Related Peptide Levels of Trigeminal Neurons in Rat Dura Mater.
doi: 10.3390/cells13080653
Figure Lengend Snippet: Figure 3. SAH reduces the proportion of CGRP-immunoreactive (IR) nerve fibers in the rat dura mater. (a) A representative image—a composite figure of CGRP-IR, red) and the pan-neuronal marker Tubulin-IR (green) immunostaining—showing the dural area around the middle meningeal artery selected for further analysis. The white square represents 1 mm2. Asterisks (*) indicate branches of the middle meningeal artery and the arrows indicate Tubulin-IR perivascular nerve fibers. (b) Representative photomicrographs were taken from a naïve control (CTL, upper panel) and an SAH animal showing CGRP-IR (left), Tubulin-IR (middle), and the composite image (right). The insets show perivascular CGRP-IR nerve fiber bundles. Densities of CGRP-IR (c) and Tubulin-IR (d) nerve fibers, as well as CGRP-IR/Tubulin-IR density ratios (e). While Tubulin-IR was not significantly different in any experimental groups, CGRP-IR and the CGRP-IR/Tubulin-IR ratios were significantly reduced in the SAH group compared to CTL and sham-injected (SHAM) animals. Data are expressed as mean ± SEM and were analyzed by one-way ANOVA (Tubulin-IR) or expressed as median with range (IQR 25–75%) analyzed by Kruskal-Wallis One Way ANOVA on Ranks (CGRP-IR, and CGRP- IR/Tubulin-IR ratios). Pairwise comparisons were performed with Tukey’s post hoc test in each analysis. *: p < 0.05 versus SHAM, #: p < 0.05 versus CTL, n = 6 in all groups.
Article Snippet: The collected samples were processed using a
Techniques: Marker, Immunostaining, Control, Injection
Journal: Cells
Article Title: Subarachnoid Hemorrhage Depletes Calcitonin Gene-Related Peptide Levels of Trigeminal Neurons in Rat Dura Mater.
doi: 10.3390/cells13080653
Figure Lengend Snippet: Figure 5. CGRP immunoreactivity and mRNA expression are reduced by SAH in the rat trigeminal ganglion (TG). (a) A representative image showing CGRP-immunoreactive cell bodies in the TG of a control rat. Inset: Strong red fluorescence shows +++ CGRP-immunoreactive neurons marked with asterisks. (b) Intensity of CGRP-immunoreactivity of individual neurons on a 0–255 grey scale range in SHAM (left) and SAH (right) animals. The number of high-intensity value cells in the SAH group is reduced. (c) Distribution of CGRP-IR neurons according to the staining intensity in SHAM and SAH groups. Data are expressed as mean ± SEM and were analyzed by One-Way ANOVA. n = 7 per each group. *: p < 0.05. (d) CGRP mRNA expression in the rat TG in the naive control (CTL, SHAM, and SAH groups). Data are expressed as mean ± SEM and were analyzed by One-Way ANOVA followed by Tukey’s post hoc test.
Article Snippet: The collected samples were processed using a
Techniques: Expressing, Control, Fluorescence, Staining