Journal: Scientific Reports

Article Title: RECQL1 as a potential therapeutic target for PARP inhibitor-resistant ovarian cancer

doi: 10.1038/s41598-025-21844-3

Figure Lengend Snippet: Representative RECQL1 immunohistochemistry images showing positive staining in the nuclei of ovarian cancer cells. ( a ) Low RECQL1 protein expression. ( b ) High RECQL1 protein expression. Original magnification: 100 × .

Article Snippet: Subsequently, IHC staining was performed with a primary anti-RECQL1 antibody (Bethyl Laboratories, Montgomery, TX, USA, catalog no. IHC-00200) diluted 1:400 according to the DAKO Envision protocol (DAKO).

Techniques: Immunohistochemistry, Staining, Expressing

Journal: Scientific Reports

Article Title: RECQL1 as a potential therapeutic target for PARP inhibitor-resistant ovarian cancer

doi: 10.1038/s41598-025-21844-3

Figure Lengend Snippet: ( a ) Kaplan–Meier curves for progression-free survival (PFS) of EOC patients. There was a trend towards shorter PFS in patients in the high RECQL1 expression group compared with that in patients in the low RECQL1 expression group, but the difference was not statistically significant ( P = 0.074). ( b ) Kaplan–Meier curves for overall survival (OS) of EOC patients. Patients in the high RECQL1 expression group showed significantly shorter OS compared with those in the low RECQL1 expression group ( P = 0.027). EOC epithelial ovarian cancer, MST median survival time, NR not reached.

Article Snippet: Subsequently, IHC staining was performed with a primary anti-RECQL1 antibody (Bethyl Laboratories, Montgomery, TX, USA, catalog no. IHC-00200) diluted 1:400 according to the DAKO Envision protocol (DAKO).

Techniques: Expressing

Journal: Scientific Reports

Article Title: RECQL1 as a potential therapeutic target for PARP inhibitor-resistant ovarian cancer

doi: 10.1038/s41598-025-21844-3

Figure Lengend Snippet: Western blot demonstrating RECQL1 protein expression patterns in the ES-2 and SKOV-3 ovarian cancer cell lines (no treatment). Knockdown of RECQL1 expression in ES-2 and SKOV-3 cells transfected with RECQL1-siRNA was tested at concentrations of 5, 10, and 15 nM. As controls, cells were transfected with negative control-siRNA and treated with transfection reagent without siRNA (mock-transfected).

Article Snippet: Subsequently, IHC staining was performed with a primary anti-RECQL1 antibody (Bethyl Laboratories, Montgomery, TX, USA, catalog no. IHC-00200) diluted 1:400 according to the DAKO Envision protocol (DAKO).

Techniques: Western Blot, Expressing, Knockdown, Transfection, Negative Control

Journal: Scientific Reports

Article Title: RECQL1 as a potential therapeutic target for PARP inhibitor-resistant ovarian cancer

doi: 10.1038/s41598-025-21844-3

Figure Lengend Snippet: Knockdown of RECQL1 expression and sensitivity to olaparib in the ES-2 and SKOV-3 ovarian cancer cell lines. The siRNA-mediated knockdown of RECQL1 expression significantly enhanced sensitivity to olaparib in ( a ) ES-2 and ( b ) SKOV-3 cells. Each assay was performed in triplicate. The data are represented as the mean ± SD. * P < 0.05 compared with the mock and negative control-siRNA transfected cells.

Article Snippet: Subsequently, IHC staining was performed with a primary anti-RECQL1 antibody (Bethyl Laboratories, Montgomery, TX, USA, catalog no. IHC-00200) diluted 1:400 according to the DAKO Envision protocol (DAKO).

Techniques: Knockdown, Expressing, Negative Control, Transfection

Journal: British journal of pharmacology

Article Title: Influence of Slco2b1-knockout and SLCO2B1-humanization on coproporphyrin I and III levels in rats.

doi: 10.1111/bph.16205

Figure Lengend Snippet: FIGURE 5 Generation of the rSlco2b1/ and the SLCO2B1+/+ rat models applying the CRISPR/Cas9-technology. (a) Cas9 mRNA and the guiding RNAs (gRNA) were co-injected into fertilized eggs which caused cleavage of the target gene and resulted in deletion of approximately 24 kb after repair in the knockout animals. Subsequently, the human SLCO2B1 coding sequence with Kozak sequence was introduced into exon 2 prior to the start codon of the endogenous sequence resulting in humanization of the gene locus. (b,c) Newborn animals were genotyped using region-specific primers (indicated with light blue arrows) to identify heterozygous or homozygous knockout (rSlco2b1/) and humanized (SLCO2B1+/+) animals. Hepatic transcript levels of (d,e) rSlco2b1 or (f,g) SLCO2B1 measured by real-time qPCR validated the knockout and the humanization and were analysed for sex differences in expression levels (*Kruskal–Wallis with uncorrected Dunn's test).

Article Snippet: 2.2.1 | Animal study Male and female wildtype, rSlco2b1 / and SLCO2B1+/+ Wistar rats (n = 7 per group, age 10–12 weeks) were generated at the Animal Facility of the University of Basel (specific pathogen-free conditions) by breeding heterozygous rSlco2b1 /wildtype and SLCO2B1+/wildtype animals, which were custom made in collaboration with Cyagen US Inc. (Santa Clara CA, USA, see Figure 5a).

Techniques: CRISPR, Injection, Knock-Out, Sequencing, Expressing

Journal: British journal of pharmacology

Article Title: Influence of Slco2b1-knockout and SLCO2B1-humanization on coproporphyrin I and III levels in rats.

doi: 10.1111/bph.16205

Figure Lengend Snippet: FIGURE 6 Comparison of coproporphyrin (CP) serum levels in wildtype, rSlco2b1-knockout and SLCO2B1-humanized rats. (a) CPIII and (b) CPI serum levels were rather unaffected by the genetic modification of rOATP2B1 as determined by comparing wildtype (filled symbol) and rSlco2b1/ (open symbol) rats of both sexes. Comparison of CP serum levels in SLCO2B1+/+ (half-filled symbol) and rSlco2b1-knockout animals revealed significantly higher levels for (c) CPIII and (d) CPI in knockout animals of female sex. For statistical analysis, a *Kruskal–Wallis (with uncorrected Dunn's test) was applied comparing animals of different genotypes but the same sex.

Article Snippet: 2.2.1 | Animal study Male and female wildtype, rSlco2b1 / and SLCO2B1+/+ Wistar rats (n = 7 per group, age 10–12 weeks) were generated at the Animal Facility of the University of Basel (specific pathogen-free conditions) by breeding heterozygous rSlco2b1 /wildtype and SLCO2B1+/wildtype animals, which were custom made in collaboration with Cyagen US Inc. (Santa Clara CA, USA, see Figure 5a).

Techniques: Comparison, Knock-Out, Modification

Journal: British journal of pharmacology

Article Title: Influence of Slco2b1-knockout and SLCO2B1-humanization on coproporphyrin I and III levels in rats.

doi: 10.1111/bph.16205

Figure Lengend Snippet: FIGURE 7 Expression of drug transporters in rat liver. mRNA expression of (a,d) rSlco1b2, (b,e) rAbcc2 and (c,f) rAbcc3 in liver of male and female wildtype (filled symbol), rSlco2b1/ (open symbol) and SLCO2B1+/+ (half-filled symbol) rats was analysed by SYBR green real-time PCR (*Kruskal–Wallis with uncorrected Dunn's test). (g,h) Western blots showing the bands of rOATP1B2, rMRP2 and rMRP3 detected in the liver of male and female wildtype, rSlco2b1/ and SLCO2B1+/+ rats. Rat GAPDH served as loading control.

Article Snippet: 2.2.1 | Animal study Male and female wildtype, rSlco2b1 / and SLCO2B1+/+ Wistar rats (n = 7 per group, age 10–12 weeks) were generated at the Animal Facility of the University of Basel (specific pathogen-free conditions) by breeding heterozygous rSlco2b1 /wildtype and SLCO2B1+/wildtype animals, which were custom made in collaboration with Cyagen US Inc. (Santa Clara CA, USA, see Figure 5a).

Techniques: Expressing, SYBR Green Assay, Real-time Polymerase Chain Reaction, Western Blot, Control

Journal: British journal of pharmacology

Article Title: Influence of Slco2b1-knockout and SLCO2B1-humanization on coproporphyrin I and III levels in rats.

doi: 10.1111/bph.16205

Figure Lengend Snippet: FIGURE 8 Immunohistochemistry of drug transporters in liver. Liver sections of male and female wildtype, rSlco2b1/ and SLCO2B1+/+ rats were probed for expression of rOATP1B2, rMRP2 and rMRP3 by immunohistochemistry. Nuclei were counterstained using Mayer's hemalum solution. Images were taken with the DMi8 microscope equipped with the MC170 HD camera and the LAS 4.8 software (white bar scale indicates 100 μm).

Article Snippet: 2.2.1 | Animal study Male and female wildtype, rSlco2b1 / and SLCO2B1+/+ Wistar rats (n = 7 per group, age 10–12 weeks) were generated at the Animal Facility of the University of Basel (specific pathogen-free conditions) by breeding heterozygous rSlco2b1 /wildtype and SLCO2B1+/wildtype animals, which were custom made in collaboration with Cyagen US Inc. (Santa Clara CA, USA, see Figure 5a).

Techniques: Immunohistochemistry, Expressing, Microscopy, Software

Journal: British journal of pharmacology

Article Title: Influence of Slco2b1-knockout and SLCO2B1-humanization on coproporphyrin I and III levels in rats.

doi: 10.1111/bph.16205

Figure Lengend Snippet: FIGURE 9 Analysis of CP transporters in the rat kidney. mRNA expression of (a) rSlco2b1, (b) SLCO2B1 and (c,d) rAbcc2 in the kidneys of male and female wildtype (solid), rSlco2b1/ (empty) and SLCO2B1+/+ (half-filled) rats were analysed by SYBR™green real-time qPCR (*Kruskal–Wallis with uncorrected Dunn's test). (e,f) Western blot of rMRP2 in the kidney of male and female wildtype, rSlco2b1/ and SLCO2B1+/+ rats. Rat GAPDH served as loading control. (g) Kidney sections of male and female wildtype, rSlco2b1/ and SLCO2B1+/+ rats were probed for expression of rMRP2 by immunohistochemistry. Nuclei were counterstained using Mayer's hemalum solution. Representative images are shown (white bar scale = 100 μm).

Article Snippet: 2.2.1 | Animal study Male and female wildtype, rSlco2b1 / and SLCO2B1+/+ Wistar rats (n = 7 per group, age 10–12 weeks) were generated at the Animal Facility of the University of Basel (specific pathogen-free conditions) by breeding heterozygous rSlco2b1 /wildtype and SLCO2B1+/wildtype animals, which were custom made in collaboration with Cyagen US Inc. (Santa Clara CA, USA, see Figure 5a).

Techniques: Expressing, SYBR Green Assay, Western Blot, Control, Immunohistochemistry