00081 Search Results


cytek northern lights clc  (Cytek Biosciences)
95
Cytek Biosciences cytek northern lights clc
Cytek Northern Lights Clc, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00081/Northern+Lights+CLC/pm39940782-461-7-7
Average 95 stars, based on 1 article reviews
cytek northern lights clc - by Bioz Stars, 2026-09
95/100 stars
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rabbit anti matrin 3 antibody  (Bethyl)
90
Bethyl rabbit anti matrin 3 antibody
Rabbit Anti Matrin 3 Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00081/Matrin+3+IHC+Antibody/pmc04380077-335-4-11
Average 90 stars, based on 1 article reviews
rabbit anti matrin 3 antibody - by Bioz Stars, 2026-09
90/100 stars
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human transferrin  (Biotium)
94
Biotium human transferrin
(A) FerroOrange staining in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2. Immunofluorescence microscopy images of FerroOrange and DAPI indicating the representative effect of three independent experiments (scale bar, 20 μm) and quantitation of immunofluorescence microscopy images. Data are presented as signal intensity relative to the sh scr condition and are the average of three independent experiments. (B) <t>Transferrin</t> uptake in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2. Data are presented as signal intensity relative to the sh scr condition and are the average of three independent experiments. (C) Surface staining of CD71 quantified using flow cytometry. Data are presented as median intensity relative to the sh scr condition and are the average of three independent experiments. (D) FerroOrange staining in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2 in the presence of FAC. (E) Quantification of relative cell number in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2 in the absence or presence of ferrostatin, ferrostatin with ferric ammonium citrate (FAC), and FAC alone. Data are presented relative to the sh scr condition for each respective treatment and are the average of three independent experiments. (F) Correlation plots between the expression of PIP4K2A from TCGA, with the indicated gene signatures. (G) Kaplan-Meier overall survival curve of patients with PDAC stratified by the expression levels of genes in the indicated gene signatures. (H–J) The MIA PaCa-2 cells were transduced with sh scr, sh α#1, or sh α#2 in the absence or presence of FAC. (H) Glucose consumption. (I) Lactate production. Data are presented relative to the cell number for each respective treatment and are representative of three independent experiments. (J) Immunoblot assessing GLUT1 and PI5P4Kα levels. Tubulin was used as a loading control. Data are representative of three independent experiments. (B–E, H, and I) Data are presented as mean ± SD. Statistical significance was calculated using unpaired two-tailed Student’s t test without (E, H, and I) or with (B–D) Welch’s correction and with pairwise Wilcoxon rank-sum test (F). ns, not significant, * p < 0.05, ** p ≤ 0.001, and *** p ≤ 0.0001.
Human Transferrin, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00081/Human+Transferrin/pmc12622763-280-33-38
Average 94 stars, based on 1 article reviews
human transferrin - by Bioz Stars, 2026-09
94/100 stars
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tips  (Opentrons Labworks)
94
Opentrons Labworks tips
(A) FerroOrange staining in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2. Immunofluorescence microscopy images of FerroOrange and DAPI indicating the representative effect of three independent experiments (scale bar, 20 μm) and quantitation of immunofluorescence microscopy images. Data are presented as signal intensity relative to the sh scr condition and are the average of three independent experiments. (B) <t>Transferrin</t> uptake in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2. Data are presented as signal intensity relative to the sh scr condition and are the average of three independent experiments. (C) Surface staining of CD71 quantified using flow cytometry. Data are presented as median intensity relative to the sh scr condition and are the average of three independent experiments. (D) FerroOrange staining in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2 in the presence of FAC. (E) Quantification of relative cell number in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2 in the absence or presence of ferrostatin, ferrostatin with ferric ammonium citrate (FAC), and FAC alone. Data are presented relative to the sh scr condition for each respective treatment and are the average of three independent experiments. (F) Correlation plots between the expression of PIP4K2A from TCGA, with the indicated gene signatures. (G) Kaplan-Meier overall survival curve of patients with PDAC stratified by the expression levels of genes in the indicated gene signatures. (H–J) The MIA PaCa-2 cells were transduced with sh scr, sh α#1, or sh α#2 in the absence or presence of FAC. (H) Glucose consumption. (I) Lactate production. Data are presented relative to the cell number for each respective treatment and are representative of three independent experiments. (J) Immunoblot assessing GLUT1 and PI5P4Kα levels. Tubulin was used as a loading control. Data are representative of three independent experiments. (B–E, H, and I) Data are presented as mean ± SD. Statistical significance was calculated using unpaired two-tailed Student’s t test without (E, H, and I) or with (B–D) Welch’s correction and with pairwise Wilcoxon rank-sum test (F). ns, not significant, * p < 0.05, ** p ≤ 0.001, and *** p ≤ 0.0001.
Tips, supplied by Opentrons Labworks, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00081/Opentrons+Filter+Tips/pm40101838-63-2-11
Average 94 stars, based on 1 article reviews
tips - by Bioz Stars, 2026-09
94/100 stars
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human donor mscs 00081  (RoosterBio)
90
RoosterBio human donor mscs 00081
(A) FerroOrange staining in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2. Immunofluorescence microscopy images of FerroOrange and DAPI indicating the representative effect of three independent experiments (scale bar, 20 μm) and quantitation of immunofluorescence microscopy images. Data are presented as signal intensity relative to the sh scr condition and are the average of three independent experiments. (B) <t>Transferrin</t> uptake in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2. Data are presented as signal intensity relative to the sh scr condition and are the average of three independent experiments. (C) Surface staining of CD71 quantified using flow cytometry. Data are presented as median intensity relative to the sh scr condition and are the average of three independent experiments. (D) FerroOrange staining in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2 in the presence of FAC. (E) Quantification of relative cell number in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2 in the absence or presence of ferrostatin, ferrostatin with ferric ammonium citrate (FAC), and FAC alone. Data are presented relative to the sh scr condition for each respective treatment and are the average of three independent experiments. (F) Correlation plots between the expression of PIP4K2A from TCGA, with the indicated gene signatures. (G) Kaplan-Meier overall survival curve of patients with PDAC stratified by the expression levels of genes in the indicated gene signatures. (H–J) The MIA PaCa-2 cells were transduced with sh scr, sh α#1, or sh α#2 in the absence or presence of FAC. (H) Glucose consumption. (I) Lactate production. Data are presented relative to the cell number for each respective treatment and are representative of three independent experiments. (J) Immunoblot assessing GLUT1 and PI5P4Kα levels. Tubulin was used as a loading control. Data are representative of three independent experiments. (B–E, H, and I) Data are presented as mean ± SD. Statistical significance was calculated using unpaired two-tailed Student’s t test without (E, H, and I) or with (B–D) Welch’s correction and with pairwise Wilcoxon rank-sum test (F). ns, not significant, * p < 0.05, ** p ≤ 0.001, and *** p ≤ 0.0001.
Human Donor Mscs 00081, supplied by RoosterBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00081/human+donor+mscs+00081/pmc05910660-355-5-14
Average 90 stars, based on 1 article reviews
human donor mscs 00081 - by Bioz Stars, 2026-09
90/100 stars
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xylene 244–00081  (FUJIFILM)
90
FUJIFILM xylene 244–00081
(A) FerroOrange staining in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2. Immunofluorescence microscopy images of FerroOrange and DAPI indicating the representative effect of three independent experiments (scale bar, 20 μm) and quantitation of immunofluorescence microscopy images. Data are presented as signal intensity relative to the sh scr condition and are the average of three independent experiments. (B) <t>Transferrin</t> uptake in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2. Data are presented as signal intensity relative to the sh scr condition and are the average of three independent experiments. (C) Surface staining of CD71 quantified using flow cytometry. Data are presented as median intensity relative to the sh scr condition and are the average of three independent experiments. (D) FerroOrange staining in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2 in the presence of FAC. (E) Quantification of relative cell number in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2 in the absence or presence of ferrostatin, ferrostatin with ferric ammonium citrate (FAC), and FAC alone. Data are presented relative to the sh scr condition for each respective treatment and are the average of three independent experiments. (F) Correlation plots between the expression of PIP4K2A from TCGA, with the indicated gene signatures. (G) Kaplan-Meier overall survival curve of patients with PDAC stratified by the expression levels of genes in the indicated gene signatures. (H–J) The MIA PaCa-2 cells were transduced with sh scr, sh α#1, or sh α#2 in the absence or presence of FAC. (H) Glucose consumption. (I) Lactate production. Data are presented relative to the cell number for each respective treatment and are representative of three independent experiments. (J) Immunoblot assessing GLUT1 and PI5P4Kα levels. Tubulin was used as a loading control. Data are representative of three independent experiments. (B–E, H, and I) Data are presented as mean ± SD. Statistical significance was calculated using unpaired two-tailed Student’s t test without (E, H, and I) or with (B–D) Welch’s correction and with pairwise Wilcoxon rank-sum test (F). ns, not significant, * p < 0.05, ** p ≤ 0.001, and *** p ≤ 0.0001.
Xylene 244–00081, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00081/xylene+244+00081/10__7554_slash_elife__54275-343-14-17
Average 90 stars, based on 1 article reviews
xylene 244–00081 - by Bioz Stars, 2026-09
90/100 stars
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report dss serial no. osq80-00081  (CANMET Mining Research)
90
CANMET Mining Research report dss serial no. osq80-00081
(A) FerroOrange staining in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2. Immunofluorescence microscopy images of FerroOrange and DAPI indicating the representative effect of three independent experiments (scale bar, 20 μm) and quantitation of immunofluorescence microscopy images. Data are presented as signal intensity relative to the sh scr condition and are the average of three independent experiments. (B) <t>Transferrin</t> uptake in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2. Data are presented as signal intensity relative to the sh scr condition and are the average of three independent experiments. (C) Surface staining of CD71 quantified using flow cytometry. Data are presented as median intensity relative to the sh scr condition and are the average of three independent experiments. (D) FerroOrange staining in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2 in the presence of FAC. (E) Quantification of relative cell number in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2 in the absence or presence of ferrostatin, ferrostatin with ferric ammonium citrate (FAC), and FAC alone. Data are presented relative to the sh scr condition for each respective treatment and are the average of three independent experiments. (F) Correlation plots between the expression of PIP4K2A from TCGA, with the indicated gene signatures. (G) Kaplan-Meier overall survival curve of patients with PDAC stratified by the expression levels of genes in the indicated gene signatures. (H–J) The MIA PaCa-2 cells were transduced with sh scr, sh α#1, or sh α#2 in the absence or presence of FAC. (H) Glucose consumption. (I) Lactate production. Data are presented relative to the cell number for each respective treatment and are representative of three independent experiments. (J) Immunoblot assessing GLUT1 and PI5P4Kα levels. Tubulin was used as a loading control. Data are representative of three independent experiments. (B–E, H, and I) Data are presented as mean ± SD. Statistical significance was calculated using unpaired two-tailed Student’s t test without (E, H, and I) or with (B–D) Welch’s correction and with pairwise Wilcoxon rank-sum test (F). ns, not significant, * p < 0.05, ** p ≤ 0.001, and *** p ≤ 0.0001.
Report Dss Serial No. Osq80 00081, supplied by CANMET Mining Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00081/report+dss+serial+no++osq80+00081/10__1590_slash_0370___44672023770080-176-8-3
Average 90 stars, based on 1 article reviews
report dss serial no. osq80-00081 - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


(A) FerroOrange staining in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2. Immunofluorescence microscopy images of FerroOrange and DAPI indicating the representative effect of three independent experiments (scale bar, 20 μm) and quantitation of immunofluorescence microscopy images. Data are presented as signal intensity relative to the sh scr condition and are the average of three independent experiments. (B) Transferrin uptake in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2. Data are presented as signal intensity relative to the sh scr condition and are the average of three independent experiments. (C) Surface staining of CD71 quantified using flow cytometry. Data are presented as median intensity relative to the sh scr condition and are the average of three independent experiments. (D) FerroOrange staining in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2 in the presence of FAC. (E) Quantification of relative cell number in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2 in the absence or presence of ferrostatin, ferrostatin with ferric ammonium citrate (FAC), and FAC alone. Data are presented relative to the sh scr condition for each respective treatment and are the average of three independent experiments. (F) Correlation plots between the expression of PIP4K2A from TCGA, with the indicated gene signatures. (G) Kaplan-Meier overall survival curve of patients with PDAC stratified by the expression levels of genes in the indicated gene signatures. (H–J) The MIA PaCa-2 cells were transduced with sh scr, sh α#1, or sh α#2 in the absence or presence of FAC. (H) Glucose consumption. (I) Lactate production. Data are presented relative to the cell number for each respective treatment and are representative of three independent experiments. (J) Immunoblot assessing GLUT1 and PI5P4Kα levels. Tubulin was used as a loading control. Data are representative of three independent experiments. (B–E, H, and I) Data are presented as mean ± SD. Statistical significance was calculated using unpaired two-tailed Student’s t test without (E, H, and I) or with (B–D) Welch’s correction and with pairwise Wilcoxon rank-sum test (F). ns, not significant, * p < 0.05, ** p ≤ 0.001, and *** p ≤ 0.0001.

Journal: Cell reports

Article Title: PI5P4Kα promotes glucose and iron acquisition to support metabolic fitness in pancreatic cancer

doi: 10.1016/j.celrep.2025.116199

Figure Lengend Snippet: (A) FerroOrange staining in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2. Immunofluorescence microscopy images of FerroOrange and DAPI indicating the representative effect of three independent experiments (scale bar, 20 μm) and quantitation of immunofluorescence microscopy images. Data are presented as signal intensity relative to the sh scr condition and are the average of three independent experiments. (B) Transferrin uptake in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2. Data are presented as signal intensity relative to the sh scr condition and are the average of three independent experiments. (C) Surface staining of CD71 quantified using flow cytometry. Data are presented as median intensity relative to the sh scr condition and are the average of three independent experiments. (D) FerroOrange staining in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2 in the presence of FAC. (E) Quantification of relative cell number in the indicated cell lines transduced with sh scr, sh α#1, or sh α#2 in the absence or presence of ferrostatin, ferrostatin with ferric ammonium citrate (FAC), and FAC alone. Data are presented relative to the sh scr condition for each respective treatment and are the average of three independent experiments. (F) Correlation plots between the expression of PIP4K2A from TCGA, with the indicated gene signatures. (G) Kaplan-Meier overall survival curve of patients with PDAC stratified by the expression levels of genes in the indicated gene signatures. (H–J) The MIA PaCa-2 cells were transduced with sh scr, sh α#1, or sh α#2 in the absence or presence of FAC. (H) Glucose consumption. (I) Lactate production. Data are presented relative to the cell number for each respective treatment and are representative of three independent experiments. (J) Immunoblot assessing GLUT1 and PI5P4Kα levels. Tubulin was used as a loading control. Data are representative of three independent experiments. (B–E, H, and I) Data are presented as mean ± SD. Statistical significance was calculated using unpaired two-tailed Student’s t test without (E, H, and I) or with (B–D) Welch’s correction and with pairwise Wilcoxon rank-sum test (F). ns, not significant, * p < 0.05, ** p ≤ 0.001, and *** p ≤ 0.0001.

Article Snippet: The following reagents were used: Polybrene (NC9840454, Santa Cruz Biotechnology), 37% Formaldehyde (RSOF0010, RICCA chemical company), Crystal Violet (C0775, Sigma), Emricasan (510230, MedKoo Biosciences), Tetramethylrhodamine Ethyl Ester Perchlorate (TMRE) (T669, Thermo Fisher Scientific), Human Transferrin, CF594 Conjugate (00084, Biotium), DAPI (422801, BioLegend), Hoechst 33342 (0219030525, MP Biomedicals), Doxycycline hyclate (D9891, Sigma).

Techniques: Staining, Transduction, Immunofluorescence, Microscopy, Quantitation Assay, Flow Cytometry, Expressing, Western Blot, Control, Two Tailed Test