00004 Search Results


chk1  (Bethyl)
86
Bethyl chk1
Vaccinia Induces a Cytoplasmic ATR/ATM-Dependent DNA Damage Response (A) Analysis of pSQ/TQ immunoreactivity (green) reveals phosphorylation of ATR/ATM substrates in response to infection with the Western Reserve (WR) strain of vaccinia virus at the indicated time post-infection or UV irradiation. The graph shows the quantitation of cells with cytoplasmic pSQ/TQ immunoreactivity. (B) Immunoblot analysis shows H2AX is phosphorylated in HeLa cells in response to UV irradiation, but not vaccinia infection. (C) Combined inhibition of ATM and ATR blocks vaccinia-induced cytoplasmic pSQ/TQ immunoreactivity. (D) Immunoblot analysis demonstrates that vaccinia induces phosphorylation of <t>Chk1</t> and Chk2. Cell fractionation demonstrates that ATR and Chk1 are phosphorylated in the cytoplasm. (E) Analysis of pSQ/TQ immunoreactivity in cells infected for 4 hr and treated with the indicated inhibitors. All error bars represent SEM from three independent experiments in which a minimum of 200 cells were counted, with ∗ p < 0.05 and ∗∗∗∗ p < 0.0001. Scale bars, 20 μm.
Chk1, supplied by Bethyl, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00004/Chk1+IHC+Antibody/pmc05437729-198-26-27
Average 86 stars, based on 1 article reviews
chk1 - by Bioz Stars, 2026-09
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acrylamide aam  (Chem Impex International)
95
Chem Impex International acrylamide aam
Vaccinia Induces a Cytoplasmic ATR/ATM-Dependent DNA Damage Response (A) Analysis of pSQ/TQ immunoreactivity (green) reveals phosphorylation of ATR/ATM substrates in response to infection with the Western Reserve (WR) strain of vaccinia virus at the indicated time post-infection or UV irradiation. The graph shows the quantitation of cells with cytoplasmic pSQ/TQ immunoreactivity. (B) Immunoblot analysis shows H2AX is phosphorylated in HeLa cells in response to UV irradiation, but not vaccinia infection. (C) Combined inhibition of ATM and ATR blocks vaccinia-induced cytoplasmic pSQ/TQ immunoreactivity. (D) Immunoblot analysis demonstrates that vaccinia induces phosphorylation of <t>Chk1</t> and Chk2. Cell fractionation demonstrates that ATR and Chk1 are phosphorylated in the cytoplasm. (E) Analysis of pSQ/TQ immunoreactivity in cells infected for 4 hr and treated with the indicated inhibitors. All error bars represent SEM from three independent experiments in which a minimum of 200 cells were counted, with ∗ p < 0.05 and ∗∗∗∗ p < 0.0001. Scale bars, 20 μm.
Acrylamide Aam, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00004/Acrylamide/norris_sam_carsten_puisis__2019__development_of_photodegradable_polymer_networks_cellular_applications_and_mathematical_models-1487-77-94
Average 95 stars, based on 1 article reviews
acrylamide aam - by Bioz Stars, 2026-09
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single channel pipette p20 gen2  (Opentrons Labworks)
94
Opentrons Labworks single channel pipette p20 gen2
Vaccinia Induces a Cytoplasmic ATR/ATM-Dependent DNA Damage Response (A) Analysis of pSQ/TQ immunoreactivity (green) reveals phosphorylation of ATR/ATM substrates in response to infection with the Western Reserve (WR) strain of vaccinia virus at the indicated time post-infection or UV irradiation. The graph shows the quantitation of cells with cytoplasmic pSQ/TQ immunoreactivity. (B) Immunoblot analysis shows H2AX is phosphorylated in HeLa cells in response to UV irradiation, but not vaccinia infection. (C) Combined inhibition of ATM and ATR blocks vaccinia-induced cytoplasmic pSQ/TQ immunoreactivity. (D) Immunoblot analysis demonstrates that vaccinia induces phosphorylation of <t>Chk1</t> and Chk2. Cell fractionation demonstrates that ATR and Chk1 are phosphorylated in the cytoplasm. (E) Analysis of pSQ/TQ immunoreactivity in cells infected for 4 hr and treated with the indicated inhibitors. All error bars represent SEM from three independent experiments in which a minimum of 200 cells were counted, with ∗ p < 0.05 and ∗∗∗∗ p < 0.0001. Scale bars, 20 μm.
Single Channel Pipette P20 Gen2, supplied by Opentrons Labworks, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00004/Single+Channel+Electronic+Pipette/pmc12117386-75-2-7
Average 94 stars, based on 1 article reviews
single channel pipette p20 gen2 - by Bioz Stars, 2026-09
94/100 stars
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alab (uk/us/06/2022/01/00004)  (ALAB Laboratories)
90
ALAB Laboratories alab (uk/us/06/2022/01/00004)
Vaccinia Induces a Cytoplasmic ATR/ATM-Dependent DNA Damage Response (A) Analysis of pSQ/TQ immunoreactivity (green) reveals phosphorylation of ATR/ATM substrates in response to infection with the Western Reserve (WR) strain of vaccinia virus at the indicated time post-infection or UV irradiation. The graph shows the quantitation of cells with cytoplasmic pSQ/TQ immunoreactivity. (B) Immunoblot analysis shows H2AX is phosphorylated in HeLa cells in response to UV irradiation, but not vaccinia infection. (C) Combined inhibition of ATM and ATR blocks vaccinia-induced cytoplasmic pSQ/TQ immunoreactivity. (D) Immunoblot analysis demonstrates that vaccinia induces phosphorylation of <t>Chk1</t> and Chk2. Cell fractionation demonstrates that ATR and Chk1 are phosphorylated in the cytoplasm. (E) Analysis of pSQ/TQ immunoreactivity in cells infected for 4 hr and treated with the indicated inhibitors. All error bars represent SEM from three independent experiments in which a minimum of 200 cells were counted, with ∗ p < 0.05 and ∗∗∗∗ p < 0.0001. Scale bars, 20 μm.
Alab (Uk/Us/06/2022/01/00004), supplied by ALAB Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00004/alab++uk+us+06+2022+01+00004+/pm40647335-321-10-13
Average 90 stars, based on 1 article reviews
alab (uk/us/06/2022/01/00004) - by Bioz Stars, 2026-09
90/100 stars
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0.00004% (4.0 ppm) sodium selenite  (research diets inc)
90
research diets inc 0.00004% (4.0 ppm) sodium selenite
Vaccinia Induces a Cytoplasmic ATR/ATM-Dependent DNA Damage Response (A) Analysis of pSQ/TQ immunoreactivity (green) reveals phosphorylation of ATR/ATM substrates in response to infection with the Western Reserve (WR) strain of vaccinia virus at the indicated time post-infection or UV irradiation. The graph shows the quantitation of cells with cytoplasmic pSQ/TQ immunoreactivity. (B) Immunoblot analysis shows H2AX is phosphorylated in HeLa cells in response to UV irradiation, but not vaccinia infection. (C) Combined inhibition of ATM and ATR blocks vaccinia-induced cytoplasmic pSQ/TQ immunoreactivity. (D) Immunoblot analysis demonstrates that vaccinia induces phosphorylation of <t>Chk1</t> and Chk2. Cell fractionation demonstrates that ATR and Chk1 are phosphorylated in the cytoplasm. (E) Analysis of pSQ/TQ immunoreactivity in cells infected for 4 hr and treated with the indicated inhibitors. All error bars represent SEM from three independent experiments in which a minimum of 200 cells were counted, with ∗ p < 0.05 and ∗∗∗∗ p < 0.0001. Scale bars, 20 μm.
0.00004% (4.0 Ppm) Sodium Selenite, supplied by research diets inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00004/0+00004+++4+0+ppm++sodium+selenite/pmc03566909-74-22-41
Average 90 stars, based on 1 article reviews
0.00004% (4.0 ppm) sodium selenite - by Bioz Stars, 2026-09
90/100 stars
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health research challenge for impact (hrci) cooperative agreement [#ghs-a-0009-00004-00]  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare health research challenge for impact (hrci) cooperative agreement [#ghs-a-0009-00004-00]
Vaccinia Induces a Cytoplasmic ATR/ATM-Dependent DNA Damage Response (A) Analysis of pSQ/TQ immunoreactivity (green) reveals phosphorylation of ATR/ATM substrates in response to infection with the Western Reserve (WR) strain of vaccinia virus at the indicated time post-infection or UV irradiation. The graph shows the quantitation of cells with cytoplasmic pSQ/TQ immunoreactivity. (B) Immunoblot analysis shows H2AX is phosphorylated in HeLa cells in response to UV irradiation, but not vaccinia infection. (C) Combined inhibition of ATM and ATR blocks vaccinia-induced cytoplasmic pSQ/TQ immunoreactivity. (D) Immunoblot analysis demonstrates that vaccinia induces phosphorylation of <t>Chk1</t> and Chk2. Cell fractionation demonstrates that ATR and Chk1 are phosphorylated in the cytoplasm. (E) Analysis of pSQ/TQ immunoreactivity in cells infected for 4 hr and treated with the indicated inhibitors. All error bars represent SEM from three independent experiments in which a minimum of 200 cells were counted, with ∗ p < 0.05 and ∗∗∗∗ p < 0.0001. Scale bars, 20 μm.
Health Research Challenge For Impact (Hrci) Cooperative Agreement [#Ghs A 0009 00004 00], supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00004/health+research+challenge+for+impact++hrci++cooperative+agreement+++ghs+a+0009+00004+00+/pm31228143-159-16-27
Average 90 stars, based on 1 article reviews
health research challenge for impact (hrci) cooperative agreement [#ghs-a-0009-00004-00] - by Bioz Stars, 2026-09
90/100 stars
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stip1 rabbit mab  (Genecopoeia)
95
Genecopoeia stip1 rabbit mab
Vaccinia Induces a Cytoplasmic ATR/ATM-Dependent DNA Damage Response (A) Analysis of pSQ/TQ immunoreactivity (green) reveals phosphorylation of ATR/ATM substrates in response to infection with the Western Reserve (WR) strain of vaccinia virus at the indicated time post-infection or UV irradiation. The graph shows the quantitation of cells with cytoplasmic pSQ/TQ immunoreactivity. (B) Immunoblot analysis shows H2AX is phosphorylated in HeLa cells in response to UV irradiation, but not vaccinia infection. (C) Combined inhibition of ATM and ATR blocks vaccinia-induced cytoplasmic pSQ/TQ immunoreactivity. (D) Immunoblot analysis demonstrates that vaccinia induces phosphorylation of <t>Chk1</t> and Chk2. Cell fractionation demonstrates that ATR and Chk1 are phosphorylated in the cytoplasm. (E) Analysis of pSQ/TQ immunoreactivity in cells infected for 4 hr and treated with the indicated inhibitors. All error bars represent SEM from three independent experiments in which a minimum of 200 cells were counted, with ∗ p < 0.05 and ∗∗∗∗ p < 0.0001. Scale bars, 20 μm.
Stip1 Rabbit Mab, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00004/STIP1+Rabbit+mAb/custom%40mab-00004%4033766539
Average 95 stars, based on 1 article reviews
stip1 rabbit mab - by Bioz Stars, 2026-09
95/100 stars
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int-00004  (Shire US Inc)
90
Shire US Inc int-00004
Vaccinia Induces a Cytoplasmic ATR/ATM-Dependent DNA Damage Response (A) Analysis of pSQ/TQ immunoreactivity (green) reveals phosphorylation of ATR/ATM substrates in response to infection with the Western Reserve (WR) strain of vaccinia virus at the indicated time post-infection or UV irradiation. The graph shows the quantitation of cells with cytoplasmic pSQ/TQ immunoreactivity. (B) Immunoblot analysis shows H2AX is phosphorylated in HeLa cells in response to UV irradiation, but not vaccinia infection. (C) Combined inhibition of ATM and ATR blocks vaccinia-induced cytoplasmic pSQ/TQ immunoreactivity. (D) Immunoblot analysis demonstrates that vaccinia induces phosphorylation of <t>Chk1</t> and Chk2. Cell fractionation demonstrates that ATR and Chk1 are phosphorylated in the cytoplasm. (E) Analysis of pSQ/TQ immunoreactivity in cells infected for 4 hr and treated with the indicated inhibitors. All error bars represent SEM from three independent experiments in which a minimum of 200 cells were counted, with ∗ p < 0.05 and ∗∗∗∗ p < 0.0001. Scale bars, 20 μm.
Int 00004, supplied by Shire US Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00004/int+00004/pm19407721-120-13-5
Average 90 stars, based on 1 article reviews
int-00004 - by Bioz Stars, 2026-09
90/100 stars
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argon-36  (ISOFLEX)
90
ISOFLEX argon-36
Vaccinia Induces a Cytoplasmic ATR/ATM-Dependent DNA Damage Response (A) Analysis of pSQ/TQ immunoreactivity (green) reveals phosphorylation of ATR/ATM substrates in response to infection with the Western Reserve (WR) strain of vaccinia virus at the indicated time post-infection or UV irradiation. The graph shows the quantitation of cells with cytoplasmic pSQ/TQ immunoreactivity. (B) Immunoblot analysis shows H2AX is phosphorylated in HeLa cells in response to UV irradiation, but not vaccinia infection. (C) Combined inhibition of ATM and ATR blocks vaccinia-induced cytoplasmic pSQ/TQ immunoreactivity. (D) Immunoblot analysis demonstrates that vaccinia induces phosphorylation of <t>Chk1</t> and Chk2. Cell fractionation demonstrates that ATR and Chk1 are phosphorylated in the cytoplasm. (E) Analysis of pSQ/TQ immunoreactivity in cells infected for 4 hr and treated with the indicated inhibitors. All error bars represent SEM from three independent experiments in which a minimum of 200 cells were counted, with ∗ p < 0.05 and ∗∗∗∗ p < 0.0001. Scale bars, 20 μm.
Argon 36, supplied by ISOFLEX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/00004/Argon-36/custom%40iso-sti-00004%4020884215
Average 90 stars, based on 1 article reviews
argon-36 - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


Vaccinia Induces a Cytoplasmic ATR/ATM-Dependent DNA Damage Response (A) Analysis of pSQ/TQ immunoreactivity (green) reveals phosphorylation of ATR/ATM substrates in response to infection with the Western Reserve (WR) strain of vaccinia virus at the indicated time post-infection or UV irradiation. The graph shows the quantitation of cells with cytoplasmic pSQ/TQ immunoreactivity. (B) Immunoblot analysis shows H2AX is phosphorylated in HeLa cells in response to UV irradiation, but not vaccinia infection. (C) Combined inhibition of ATM and ATR blocks vaccinia-induced cytoplasmic pSQ/TQ immunoreactivity. (D) Immunoblot analysis demonstrates that vaccinia induces phosphorylation of Chk1 and Chk2. Cell fractionation demonstrates that ATR and Chk1 are phosphorylated in the cytoplasm. (E) Analysis of pSQ/TQ immunoreactivity in cells infected for 4 hr and treated with the indicated inhibitors. All error bars represent SEM from three independent experiments in which a minimum of 200 cells were counted, with ∗ p < 0.05 and ∗∗∗∗ p < 0.0001. Scale bars, 20 μm.

Journal: Cell Reports

Article Title: Cytoplasmic ATR Activation Promotes Vaccinia Virus Genome Replication

doi: 10.1016/j.celrep.2017.04.025

Figure Lengend Snippet: Vaccinia Induces a Cytoplasmic ATR/ATM-Dependent DNA Damage Response (A) Analysis of pSQ/TQ immunoreactivity (green) reveals phosphorylation of ATR/ATM substrates in response to infection with the Western Reserve (WR) strain of vaccinia virus at the indicated time post-infection or UV irradiation. The graph shows the quantitation of cells with cytoplasmic pSQ/TQ immunoreactivity. (B) Immunoblot analysis shows H2AX is phosphorylated in HeLa cells in response to UV irradiation, but not vaccinia infection. (C) Combined inhibition of ATM and ATR blocks vaccinia-induced cytoplasmic pSQ/TQ immunoreactivity. (D) Immunoblot analysis demonstrates that vaccinia induces phosphorylation of Chk1 and Chk2. Cell fractionation demonstrates that ATR and Chk1 are phosphorylated in the cytoplasm. (E) Analysis of pSQ/TQ immunoreactivity in cells infected for 4 hr and treated with the indicated inhibitors. All error bars represent SEM from three independent experiments in which a minimum of 200 cells were counted, with ∗ p < 0.05 and ∗∗∗∗ p < 0.0001. Scale bars, 20 μm.

Article Snippet: Cells were labeled with primary antibodies against Phospho-(Ser/Thr) ATM/ATR (New England Biolabs, 2851), RPA (Santa Cruz Biotechnology, SC-56770), Ku70 (Bethel Laboratories, IHC-00723), ATR (Bethel Laboratories, A300-138A), Chk1 (Bethyl Laboratories, IHC-00004), RPA phospho-Ser33 (Bethyl Laboratories, IHC-00421), INTS7 (GeneTex, GTX82516), E9 monoclonal , and I3 polyclonal ( ).

Techniques: Phospho-proteomics, Infection, Western Blot, Virus, Irradiation, Quantitation Assay, Inhibition, Cell Fractionation

ATR and Chk1 Are Required for Vaccinia Genome Replication (A) Immunoblot analysis demonstrates that ATR (ATRi VE-821), but not ATM (ATMi KU55633), inhibits late (F13 and A27), but not early (H5), viral protein expression 8 hr post-infection. (B) Immunofluorescence analysis of cells infected for 8 hr reveals that ATRi inhibits expression of RFP-A3, a late viral core protein. Scale bar, 10 μm. (C) ATRi inhibits viral production 8 hpi. (D) Quantification of viral genome copy number in cells infected for 6 hr in the presence of AraC or ATRi 1 hr pre- (−1) or post- (+1) infection. Both AraC and ATRi inhibit expression of F13 (late), but not H5 (early). (E) Removal of AraC 4 hr post-infection in the presence, but not absence, of ATRi reduces genome replication and late (F13, A27), but not early (H5), viral protein expression. (F) Removal of AraC 4 hr post-infection in the presence of the indicated inhibitors reduces genome replication and late gene expression. All error bars represent SEM from three independent experiments, with ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001.

Journal: Cell Reports

Article Title: Cytoplasmic ATR Activation Promotes Vaccinia Virus Genome Replication

doi: 10.1016/j.celrep.2017.04.025

Figure Lengend Snippet: ATR and Chk1 Are Required for Vaccinia Genome Replication (A) Immunoblot analysis demonstrates that ATR (ATRi VE-821), but not ATM (ATMi KU55633), inhibits late (F13 and A27), but not early (H5), viral protein expression 8 hr post-infection. (B) Immunofluorescence analysis of cells infected for 8 hr reveals that ATRi inhibits expression of RFP-A3, a late viral core protein. Scale bar, 10 μm. (C) ATRi inhibits viral production 8 hpi. (D) Quantification of viral genome copy number in cells infected for 6 hr in the presence of AraC or ATRi 1 hr pre- (−1) or post- (+1) infection. Both AraC and ATRi inhibit expression of F13 (late), but not H5 (early). (E) Removal of AraC 4 hr post-infection in the presence, but not absence, of ATRi reduces genome replication and late (F13, A27), but not early (H5), viral protein expression. (F) Removal of AraC 4 hr post-infection in the presence of the indicated inhibitors reduces genome replication and late gene expression. All error bars represent SEM from three independent experiments, with ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001.

Article Snippet: Cells were labeled with primary antibodies against Phospho-(Ser/Thr) ATM/ATR (New England Biolabs, 2851), RPA (Santa Cruz Biotechnology, SC-56770), Ku70 (Bethel Laboratories, IHC-00723), ATR (Bethel Laboratories, A300-138A), Chk1 (Bethyl Laboratories, IHC-00004), RPA phospho-Ser33 (Bethyl Laboratories, IHC-00421), INTS7 (GeneTex, GTX82516), E9 monoclonal , and I3 polyclonal ( ).

Techniques: Western Blot, Expressing, Infection, Immunofluorescence, Gene Expression