Journal: Molecular Cell

Article Title: A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation

doi: 10.1016/j.molcel.2021.05.032

Figure Lengend Snippet:

Article Snippet: 3′,5′-cyclic AMP.Na (cAMP) , Chem-Impex Int’l Inc. , Cat#00008.

Techniques: Recombinant, Software

Journal: Cellular and Molecular Life Sciences

Article Title: The human ABCB6 protein is the functional homologue of HMT-1 proteins mediating cadmium detoxification

doi: 10.1007/s00018-019-03105-5

Figure Lengend Snippet: ABCB6 confers cadmium tolerance in hmt - 1 Δ mutant S. pombe . a SDS-PAGE and immunoblot analysis of yeast cells expressing HMT-1-HA (lane 1), HMT-1-GFP (lane 2), ABCB6 (lane 3), ABCB6-KM (lane 4) or ABCB6-GFP (lane 5). Expression of SpHMT-1 was revealed using anti-HA antibody; ABCB6 was labeled by the ABCB6-567 antibody; EGFP tagged proteins were labeled by an anti-EGFP antibody. b ABCB6-GFP (green) localizes to vacuoles (red) of S. pombe . Hmt - 1 -deleted S. pombe was transformed with pREP1-HMT-1-GFP or ABCB6-GFP; vacuoles were stained with FM 4–64. Insets show individual cells. Scale bar 10 μm. c Wild-type S. pombe cells transformed with empty pREP1 vector (WT); hmt - 1 Δ mutant cells transformed with empty pREP1 vector ( hmt - 1 Δ), pREP1-HMT-1-HA ( hmt - 1 Δ/SpHMT-1-HA), pREP1-ABCB6 ( hmt - 1 Δ/ABCB6) or pREP1-ABCB6-KM ( hmt - 1 Δ/ABCB6-KM) were grown overnight to an A 600nm of 1.8. Aliquots of the cell suspensions were then serially diluted and spotted onto solid EMM supplemented with adenine, uracil and the indicated concentrations of CdCl 2 . Colonies were visualized after incubating the plates for 8 days at 30 °C. d Transformants were grown overnight to an A 600nm of 0.8–1. Aliquots of 100-μL were inoculated into 2 mL of the same medium containing CdCl 2 at the indicated concentrations. A 600nm was measured after growth at 30 °C for 72 h. Values, expressed as viability (%), were normalized to untreated control ( n = 3)

Article Snippet: The S. pombe wild-type strain BG_00008 (ade6-M216, ura4-D18, leu1-32) and the hmt - 1 -deleted mutant strain BG_H4691 (ade6-M216, ura4-D18, leu1-32) was a generous gift from R. Lill (Philipps-Universität Marburg).

Techniques: Mutagenesis, SDS Page, Western Blot, Expressing, Labeling, Transformation Assay, Staining, Plasmid Preparation, Control

Journal: Cellular and Molecular Life Sciences

Article Title: The human ABCB6 protein is the functional homologue of HMT-1 proteins mediating cadmium detoxification

doi: 10.1007/s00018-019-03105-5

Figure Lengend Snippet: ABCB6 restores vacuolar Cd levels in hmt - 1 Δ mutant S. pombe . Yeast cells lacking SpHMT-1 ( hmt - 1 Δ) were engineered to express ABCB6, ABCB6-KM or SpHMT-1. Transformed cells were cultured for 18 h in growth medium supplemented with 20 μM CdCl 2 . Intact vacuoles were isolated by differential centrifugation; the Cd content of the isolated vacuoles was quantified by GFAAS. Data show average Cd levels relative to control calculated from independent experiments ( n = 3)

Article Snippet: The S. pombe wild-type strain BG_00008 (ade6-M216, ura4-D18, leu1-32) and the hmt - 1 -deleted mutant strain BG_H4691 (ade6-M216, ura4-D18, leu1-32) was a generous gift from R. Lill (Philipps-Universität Marburg).

Techniques: Mutagenesis, Transformation Assay, Cell Culture, Isolation, Centrifugation, Control