βcgrp Search Results


90
Neo MPS Inc βcgrp peptide
βcgrp Peptide, supplied by Neo MPS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
βcgrp peptide - by Bioz Stars, 2026-05
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90
Bachem βcgrp(1–37)
βcgrp(1–37), supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
GL Biochem βcgrp
βcgrp, supplied by GL Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Phoenix Pharmaceuticals βcgrp
Fig. 5. <t>CGRP</t> increases NFATc2 binding to the IL-17 promoter region. (A) ChIP was performed using anti-NFATc2, anti-RORγt or control IgG antibodies. PCR was performed using specific primers for the IL-17 promoter. Th cells were stimulated with 1 <t>nM</t> <t>αCGRP</t> (α) and βCGRP (β). (B) The histogram shows the results of quantitative analyses of (A) using real-time PCR analysis. Values are presented as mean ± SD (n = 4; *P < 0.05). (C) ChIP was performed as in (A). Th cells were stimulated with 50 μM 6-bnz-cAMP (6-bnz) or 50 μM 8-CPT-cAMP (8-CPT) in the presence of TGF-β and IL-6. (D) The histogram shows the results of quantitative analyses of (C) using real-time PCR analysis. Values are pre- sented as mean ± SD (n = 3; **P < 0.01). (E) The effect of NFAT inhibitor (50 μM) on CGRP-induced IL-17 mRNA expression was determined by real-time PCR. Values are presented as means ± SD (n = 3). *P < 0.05.
βcgrp, supplied by Phoenix Pharmaceuticals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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Fig. 5. CGRP increases NFATc2 binding to the IL-17 promoter region. (A) ChIP was performed using anti-NFATc2, anti-RORγt or control IgG antibodies. PCR was performed using specific primers for the IL-17 promoter. Th cells were stimulated with 1 nM αCGRP (α) and βCGRP (β). (B) The histogram shows the results of quantitative analyses of (A) using real-time PCR analysis. Values are presented as mean ± SD (n = 4; *P < 0.05). (C) ChIP was performed as in (A). Th cells were stimulated with 50 μM 6-bnz-cAMP (6-bnz) or 50 μM 8-CPT-cAMP (8-CPT) in the presence of TGF-β and IL-6. (D) The histogram shows the results of quantitative analyses of (C) using real-time PCR analysis. Values are pre- sented as mean ± SD (n = 3; **P < 0.01). (E) The effect of NFAT inhibitor (50 μM) on CGRP-induced IL-17 mRNA expression was determined by real-time PCR. Values are presented as means ± SD (n = 3). *P < 0.05.

Journal: International immunology

Article Title: Calcitonin gene-related peptide enhances experimental autoimmune encephalomyelitis by promoting Th17-cell functions.

doi: 10.1093/intimm/dxs075

Figure Lengend Snippet: Fig. 5. CGRP increases NFATc2 binding to the IL-17 promoter region. (A) ChIP was performed using anti-NFATc2, anti-RORγt or control IgG antibodies. PCR was performed using specific primers for the IL-17 promoter. Th cells were stimulated with 1 nM αCGRP (α) and βCGRP (β). (B) The histogram shows the results of quantitative analyses of (A) using real-time PCR analysis. Values are presented as mean ± SD (n = 4; *P < 0.05). (C) ChIP was performed as in (A). Th cells were stimulated with 50 μM 6-bnz-cAMP (6-bnz) or 50 μM 8-CPT-cAMP (8-CPT) in the presence of TGF-β and IL-6. (D) The histogram shows the results of quantitative analyses of (C) using real-time PCR analysis. Values are pre- sented as mean ± SD (n = 3; **P < 0.01). (E) The effect of NFAT inhibitor (50 μM) on CGRP-induced IL-17 mRNA expression was determined by real-time PCR. Values are presented as means ± SD (n = 3). *P < 0.05.

Article Snippet: N6-benzoyl-cAMP (6-bnz-cAMP) and 8-(4-Chlorophenylthio)-2′-O-Methyl-cAMP (8-CPT-cAMP) were purchased from Calbiochem (San Diego, CA) and PKA inhibitor H89 and NFAT inhibitor were purchased from Sigma Aldrich (St. Louis, MO). αCGRP was purchased from Peptide Institute (Osaka, Japan) and βCGRP was purchased from Phoenix Pharmaceuticals, Inc. (Burlingame, CA).

Techniques: Binding Assay, Control, Real-time Polymerase Chain Reaction, Expressing