Journal: Frontiers in Pharmacology

Article Title: Changes in Expression and Cellular Localization of Rat Skeletal Muscle ClC-1 Chloride Channel in Relation to Age, Myofiber Phenotype and PKC Modulation

doi: 10.3389/fphar.2020.00714

Figure Lengend Snippet: Subcellular localization of ClC-1 in EDL and SOL muscle fibers in relation to rat age obtained by epifluorescence microscopy analysis (16× magnification). The images show double-immunofluorescence with β-DG (red) and ClC-1 (green) in EDL (A) and SOL (B) fibers.

Article Snippet: The antibodies against ClC-1 and beta-dystroglycan (β-DG) (Novocastra cod: NCL-b-DG) were diluted 1:200 and 1:500, respectively, in PBS-gelation.

Techniques: Epifluorescence Microscopy, Immunofluorescence

Journal: Frontiers in Pharmacology

Article Title: Changes in Expression and Cellular Localization of Rat Skeletal Muscle ClC-1 Chloride Channel in Relation to Age, Myofiber Phenotype and PKC Modulation

doi: 10.3389/fphar.2020.00714

Figure Lengend Snippet: Quantification of colocalization of ClC-1 and β-DG in the fibers of EDL and SOL muscles in relation to age obtained using Pearson’s correlation coefficient and epifluorescence microscopy. The histograms show the mean ± SEM of Pcc-R obtained by comparing the distribution of ClC-1 and β-DG in three different images measured using epifluorescence microscopy (16× magnification) for each age analyzed. Significant differences between groups were evaluated using one-way ANOVA (Pcc-R in EDL muscles F=25.34, R=0.91; Pcc-R in SOL muscles F=34.72, R=0.92). Fisher’s test was used to evaluate the individual differences between groups. Significant differences (P <0.05) are expressed for ° vs. 7 Days and • vs. 12 Days.

Article Snippet: The antibodies against ClC-1 and beta-dystroglycan (β-DG) (Novocastra cod: NCL-b-DG) were diluted 1:200 and 1:500, respectively, in PBS-gelation.

Techniques: Muscles, Epifluorescence Microscopy

Journal: Frontiers in Pharmacology

Article Title: Changes in Expression and Cellular Localization of Rat Skeletal Muscle ClC-1 Chloride Channel in Relation to Age, Myofiber Phenotype and PKC Modulation

doi: 10.3389/fphar.2020.00714

Figure Lengend Snippet: Subcellular localization of ClC-1 in EDL and SOL muscle fibers in relation to rat age using high-resolution confocal microscopy (20× magnification). Images show a transversal section of (EDL (A) and SOL (B) rat muscles 7P and 2M old obtained by double-immunofluorescence with ClC-1 (green) and β-DG (red).

Article Snippet: The antibodies against ClC-1 and beta-dystroglycan (β-DG) (Novocastra cod: NCL-b-DG) were diluted 1:200 and 1:500, respectively, in PBS-gelation.

Techniques: Confocal Microscopy, Muscles, Immunofluorescence

Journal: Frontiers in Pharmacology

Article Title: Changes in Expression and Cellular Localization of Rat Skeletal Muscle ClC-1 Chloride Channel in Relation to Age, Myofiber Phenotype and PKC Modulation

doi: 10.3389/fphar.2020.00714

Figure Lengend Snippet: Quantification of colocalization of ClC-1 and β-DG in the fibers of EDL and SOL muscles in relation to age obtained using Pearson’s correlation coefficient and confocal microscopy. The histograms show the mean ± SEM of Pcc-R obtained by comparing the distribution of ClC-1 and β-DG in three different images measured using confocal microscopy (20× magnification) for each age analyzed. Significant differences between groups were evaluated using Student’s t-test (P <0.0001) are expressed for ° vs. 7 Days.

Article Snippet: The antibodies against ClC-1 and beta-dystroglycan (β-DG) (Novocastra cod: NCL-b-DG) were diluted 1:200 and 1:500, respectively, in PBS-gelation.

Techniques: Muscles, Confocal Microscopy