Journal: Drug Delivery

Article Title: Combined photodynamic-chemotherapy investigation of cancer cells using carbon quantum dot-based drug carrier system

doi: 10.1080/10717544.2020.1765431

Figure Lengend Snippet: Overall representation for the preparation of 5-ALA-CQD-Glu-β-CD nanocarrier.

Article Snippet: 5-ALA was tagged with CQD functionalized mono-(5-BOC-protected-glutamine-6-deoxy) β-cyclodextrin via EDC/NHS chemistry.

Techniques:

Journal: Drug Delivery

Article Title: Combined photodynamic-chemotherapy investigation of cancer cells using carbon quantum dot-based drug carrier system

doi: 10.1080/10717544.2020.1765431

Figure Lengend Snippet: FTIR spectra of (a) Ts-β-CD, (b) BOC-Glu, (c) Ts-β-CD- g -BOC-Glu, (d) CQD, (e) CQD-Glu-β-CD, (f) 5-ALA-CQD-Glu-β-CD, and (g) DOX/5-ALA-CQD-Glu-β-CD nanocarrier.

Article Snippet: 5-ALA was tagged with CQD functionalized mono-(5-BOC-protected-glutamine-6-deoxy) β-cyclodextrin via EDC/NHS chemistry.

Techniques:

Journal: Drug Delivery

Article Title: Combined photodynamic-chemotherapy investigation of cancer cells using carbon quantum dot-based drug carrier system

doi: 10.1080/10717544.2020.1765431

Figure Lengend Snippet: XRD pattern of (a) Ts-β-CD, (b) BOC-Glu, (c) Ts-β-CD- g -BOC-Glu, (d) CQD (e) CQD-Glu-β-CD, (f) 5-ALA-CQD-Glu-β-CD, and (f) DOX/5-ALA-CQD-Glu-β-CD nanocarrier.

Article Snippet: 5-ALA was tagged with CQD functionalized mono-(5-BOC-protected-glutamine-6-deoxy) β-cyclodextrin via EDC/NHS chemistry.

Techniques:

Journal: Drug Delivery

Article Title: Combined photodynamic-chemotherapy investigation of cancer cells using carbon quantum dot-based drug carrier system

doi: 10.1080/10717544.2020.1765431

Figure Lengend Snippet: TEM images with SEAD spectrum of (a) CQD, (b) 5-ALA-CQD-BOC-Glu-β-CD, and (c) DOX/5-ALA-CQD-BOC-Glu-β-CD nanocarrier.

Article Snippet: 5-ALA was tagged with CQD functionalized mono-(5-BOC-protected-glutamine-6-deoxy) β-cyclodextrin via EDC/NHS chemistry.

Techniques:

Journal: Drug Delivery

Article Title: Combined photodynamic-chemotherapy investigation of cancer cells using carbon quantum dot-based drug carrier system

doi: 10.1080/10717544.2020.1765431

Figure Lengend Snippet: The AFM images of 2D, 3D, and topographical images of (a–c) CQD, (d–f) 5-ALA-CQD-BOC-Glu-β-CD, and (g–i) DOX/5-ALA-CQD-BOC-Glu-β-CD nanocarrier.

Article Snippet: 5-ALA was tagged with CQD functionalized mono-(5-BOC-protected-glutamine-6-deoxy) β-cyclodextrin via EDC/NHS chemistry.

Techniques:

Journal: Drug Delivery

Article Title: Combined photodynamic-chemotherapy investigation of cancer cells using carbon quantum dot-based drug carrier system

doi: 10.1080/10717544.2020.1765431

Figure Lengend Snippet: UV–vis spectrum (a) encapsulation efficiency and (b) loading capacity of DOX/5-ALA-CQD-BOC-Glu-β-CD nanocarrier.

Article Snippet: 5-ALA was tagged with CQD functionalized mono-(5-BOC-protected-glutamine-6-deoxy) β-cyclodextrin via EDC/NHS chemistry.

Techniques:

Journal: Drug Delivery

Article Title: Combined photodynamic-chemotherapy investigation of cancer cells using carbon quantum dot-based drug carrier system

doi: 10.1080/10717544.2020.1765431

Figure Lengend Snippet: In vitro drug release behavior of 5-ALA and DOX drug from DOX/5-ALA-CQD-BOC-Glu-β-CD under three different physiological pHs (A) 2.8, (B) 5.5, (C) 6.8 at 27 °C for 24 h (A–C). Cumulative drug release (%) behavior (D–F).

Article Snippet: 5-ALA was tagged with CQD functionalized mono-(5-BOC-protected-glutamine-6-deoxy) β-cyclodextrin via EDC/NHS chemistry.

Techniques: In Vitro

Journal: Drug Delivery

Article Title: Combined photodynamic-chemotherapy investigation of cancer cells using carbon quantum dot-based drug carrier system

doi: 10.1080/10717544.2020.1765431

Figure Lengend Snippet: In vitro morphological changes of fibroblast (WS-1) cell line treated with 5-ALA CQD-Glu-β-CD and DOX/5-ALA-CQD-Glu-β-CD nanocarrier in a various time-dependent manner such as 6 h, 12 h, 18 h, and 24 h and IC 50 concentration of 60 μg/mL at scale bar: ∼50 μm.

Article Snippet: 5-ALA was tagged with CQD functionalized mono-(5-BOC-protected-glutamine-6-deoxy) β-cyclodextrin via EDC/NHS chemistry.

Techniques: In Vitro, Concentration Assay

Journal: Drug Delivery

Article Title: Combined photodynamic-chemotherapy investigation of cancer cells using carbon quantum dot-based drug carrier system

doi: 10.1080/10717544.2020.1765431

Figure Lengend Snippet: In vitro morphological changes by the chemo-photodynamic effect of breast cancer (MCF-7) cell treated with DOX/CQD-Glu-β-CD, 5-ALA-CQD-Glu-β-CD, and DOX/5-ALA-CQD-Glu-β-CD nanocarrier with and without irradiation in various time-dependent manners such as 6 h, 12 h, 18 h, and 24 h and IC 50 concentration of 60 μg/mL at scale bar: ∼100 μm.

Article Snippet: 5-ALA was tagged with CQD functionalized mono-(5-BOC-protected-glutamine-6-deoxy) β-cyclodextrin via EDC/NHS chemistry.

Techniques: In Vitro, Irradiation, Concentration Assay

Journal: Drug Delivery

Article Title: Combined photodynamic-chemotherapy investigation of cancer cells using carbon quantum dot-based drug carrier system

doi: 10.1080/10717544.2020.1765431

Figure Lengend Snippet: Fluorescent microscopic images of breast cancer (MCF-7) cell line incubated with DOX-loaded CQD-Glu-β-CD, and DOX-loaded 5-ALA-CQD-Glu-β-CD nanocarrier without and with laser irradiation in a various time-dependent manner such as 6 h, 12 h, 18 h, and 24 h and IC 50 concentration of 60 μg/mL respectively at scale bar: ∼30 μm.

Article Snippet: 5-ALA was tagged with CQD functionalized mono-(5-BOC-protected-glutamine-6-deoxy) β-cyclodextrin via EDC/NHS chemistry.

Techniques: Incubation, Irradiation, Concentration Assay

Journal: Drug Delivery

Article Title: Combined photodynamic-chemotherapy investigation of cancer cells using carbon quantum dot-based drug carrier system

doi: 10.1080/10717544.2020.1765431

Figure Lengend Snippet: ROS level evaluation under irradiation in 6 h incubation with DOX/5-ALA-CQD-Glu-β-CD nanocarrier in breast cancer (MCF-7) cell line without (a–c) and with radiation (d–f) scale bar: ∼10 μm.

Article Snippet: 5-ALA was tagged with CQD functionalized mono-(5-BOC-protected-glutamine-6-deoxy) β-cyclodextrin via EDC/NHS chemistry.

Techniques: Irradiation, Incubation

Journal: Drug Delivery

Article Title: Combined photodynamic-chemotherapy investigation of cancer cells using carbon quantum dot-based drug carrier system

doi: 10.1080/10717544.2020.1765431

Figure Lengend Snippet: Qualitative assessment of fluorescence intensity associated with MCF-7 cells by the incubation of coumarin-6 loaded CQD-Glu-β-CD nanocarrier for 1 (a), 3 (b), 6 (c), and 12 h using blue filter (excitation wavelength 460–490 nm, emission 470 nm) in inverted fluorescence microscopy.

Article Snippet: 5-ALA was tagged with CQD functionalized mono-(5-BOC-protected-glutamine-6-deoxy) β-cyclodextrin via EDC/NHS chemistry.

Techniques: Fluorescence, Incubation, Microscopy

Journal: The Journal of Physiology

Article Title: Inhibition of cardiac pacemaker channel hHCN2 depends on intercalation of lipopolysaccharide into channel-containing membrane microdomains

doi: 10.1113/jphysiol.2013.268540

Figure Lengend Snippet: A, current traces recorded after incubation of cells with 10 mm βCD (90 min). B, current traces obtained after co-incubation of cells with 10 mm βCD and 50 μg ml−1 LPS (90 min). C, mean V0.5 recorded under control conditions (open bar, n = 15), in the presence of 50 μg ml−1 LPS (black bar, n = 8), after incubation of cells with 10 mm βCD (60–120 min, light grey bar, n = 10) and after co-incubation of cells with 10 mm βCD and 50 μg ml−1 LPS (60–120 min, dark grey bar, n = 13). βCD shifts V0.5 of IhHCN2 to less negative potentials. However, in the presence of βCD, LPS does not modulate IhHCN2. *P < 0.05 versus control.

Article Snippet: In order to verify a physical interaction of βCD with LPS, we used a βCD polymer (βCD monomers cross-linked by epichlorohydrin; sc-296103; Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

Techniques: Incubation, Control

Journal: The Journal of Physiology

Article Title: Inhibition of cardiac pacemaker channel hHCN2 depends on intercalation of lipopolysaccharide into channel-containing membrane microdomains

doi: 10.1113/jphysiol.2013.268540

Figure Lengend Snippet: A, current traces recorded in the presence of 10 mm βCD. B, current traces recorded in the presence of 50 μg ml−1 LPS, 60 min after washout of βCD. C, mean V0.5 obtained in the presence of 10 mm βCD (white bar, n = 9), 60 min after washout of βCD (grey bar, n = 7) and in the presence of 50 μg ml−1 LPS, 60 min after washout of βCD (black bar, n = 9). The effect of βCD on V0.5 of IhHCN2 is still present 60 min after βCD washout, indicating that cell membranes are still cholesterol-depleted. The LPS-dependent shift of V0.5 to more negative potentials is reduced but still detectable after βCD is washed out. D, schematic diagram of the interaction of the acyl chains of lipid A with the hydrophobic cavity of βCD (according to Sakata et al. 2011). *P < 0.05 versus βCD washout.

Article Snippet: In order to verify a physical interaction of βCD with LPS, we used a βCD polymer (βCD monomers cross-linked by epichlorohydrin; sc-296103; Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

Techniques: