Journal: BMC Neuroscience

Article Title: Glycine receptors expression in rat spinal cord and dorsal root ganglion in prostaglandin E2 intrathecal injection models

doi: 10.1186/s12868-018-0470-8

Figure Lengend Snippet: a Western blot and quantitative analysis of b gephyrin, c GlyRα1, and d GlyRα3 protein expressions in L5 DRG at 1 h and 5 h after PGE2 intrathecal injection. The gephyrin, GlyRα1 and GlyRα3 protein expressions were significantly decreased in the PGE2 group at 5 h. Confocal microscopic imaging showed expression of e gephyrin, f GlyRα1 and g GlyRα3 localized mainly in the neuron cells. Each group had n = 3–6 rats. Scale Bars: 20 μm; ** p < 0.01, and *** p < 0.001, One-way ANOVA, followed by the LSD test

Article Snippet: The membranes were firstly blocked with 5% milk in phosphate-buffered saline (PBS) with 0.1% Tween-20 for 1 h at room temperature, and then probed overnight at 4 °C with Rabbit anti-GlyRα1 Polyclonal Antibody (1:1000, AGR-001, Alomone Labs, Israel), Rabbit anti-gephyrin Polyclonal Antibody (1:2000, AIP-005, Alomone Labs, Israel), goat Anti-GlyRα3 polyclonal Antibody (1:500, SC-17282, Santa Cruz Biotechnology, Santa Cruz, CA, USA), or mouse anti-actin monoclonal antibody (1:10,000, MAB1501, Indianapolis, IN, USA) primary antibody to detect the expression of GlyRα1, GlyRα3 and gephyrin in the DRG and spinal cord.

Techniques: Western Blot, Injection, Imaging, Expressing

Journal: Journal of neurochemistry

Article Title: Neurochemical Organization of the Ventral Striatum’s Olfactory Tubercle

doi: 10.1111/jnc.14919

Figure Lengend Snippet: (Top) Diagram of a rat brain, including a coronal section indicating the nucleus accumbens, ventral pallidum, and olfactory tubercle (beige shaded region). (Bottom) Enlarged view of the olfactory tubercle, with layer 1 depicted in pink, layer 2 depicted in orange, and layer 3 depicted in yellow. An island of Calleja is depicted in red. Some examples of different cell types found in the olfactory tubercle are illustrated. Based in part upon (Xiong and Wesson 2016). M, medial; D, dorsal; MSN, medium spiny neuron; GC, granule cell; CIN, cholinergic interneuron.

Article Snippet: Strong immunoreactivity for the α1 and β2 subunits is restricted to the ventral pallidum and possibly the ICj, but is weak to moderate throughout the rest of the OT ( Schwarzer et al. 2001 ).

Techniques: