zymoclean large fragment dna recovery kit  (Zymo Research)


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    Name:
    Zymoclean Large Fragment DNA Recovery
    Description:
    The Zymoclean Large Fragment DNA Recovery Kit provides a streamlined method for the rapid purification and concentration of high quality large sized DNA from agarose gels Simply add the specially formulated Agarose Dissolving Buffer ADB to the gel slice containing a DNA sample let dissolve and then transfer to the supplied Zymo Spin IC XL Column There is no need for organic denaturants or chloroform Instead the product utilizes unique spin column technology to yield high quality purified DNA in just minutes DNA purified using the Zymoclean Large Fragment DNA Recovery Kit is ideal for PCR sequencing endonuclease digestion ligation etc The entire procedure typically takes about 15 minutes
    Catalog Number:
    d4046
    Price:
    None
    Category:
    Life Science Reagents and Media
    Applications:
    Large Fragment DNA Purification
    Size:
    25 units
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    Structured Review

    Zymo Research zymoclean large fragment dna recovery kit
    Zymoclean Large Fragment DNA Recovery
    The Zymoclean Large Fragment DNA Recovery Kit provides a streamlined method for the rapid purification and concentration of high quality large sized DNA from agarose gels Simply add the specially formulated Agarose Dissolving Buffer ADB to the gel slice containing a DNA sample let dissolve and then transfer to the supplied Zymo Spin IC XL Column There is no need for organic denaturants or chloroform Instead the product utilizes unique spin column technology to yield high quality purified DNA in just minutes DNA purified using the Zymoclean Large Fragment DNA Recovery Kit is ideal for PCR sequencing endonuclease digestion ligation etc The entire procedure typically takes about 15 minutes
    https://www.bioz.com/result/zymoclean large fragment dna recovery kit/product/Zymo Research
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zymoclean large fragment dna recovery kit - by Bioz Stars, 2021-03
    99/100 stars

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    Synthesized:

    Article Title: User-Friendly Reverse Genetics System for Modification of the Right End of Fowl Adenovirus 4 Genome
    Article Snippet: Construct Intermediate Plasmid pKFAV4AP Plasmid pKFAV4 (46.2 kb) contained two AvrII sites. .. Two single-stranded oligonucleotides surrounding the AvrII sites in pKFAV4 were synthesized ( ) and fused to form a double stranded DNA (AvrII-PacI, 96 bp in length) through DNA polymerasemediated extension reaction (Q5 High-Fidelity DNA Polymerase, Cat. M0491S; New England Biolabs, Ipswich, Massachusetts, USA), and a PacI site (ttaattaa) was added in the middle to demarcate the two AvrII-centered parts. pKFAV4 was digested with AvrII, the small fragment (16.7 kb) was recovered from agarose gel (Zymoclean Large Fragment DNA Recovery Kit, Cat.D4045; Zymo Research, Irvine, CA, USA) and mixed with AvrII-PacI fragment, and Gibson assembly was performed to generate an intermediate plasmid pKFAV4AP ( pKFAV4 A vrII- P acI) (NEBuilderHiFi DNA Assembly Master Mix, Cat. E2621; New England Biolabs). .. Delete ORF1-ORF2 in pKFAV4 pKFAV4AP was digested with AgeI/NheI, and the fragment of 4942 bp was recovered; PCR was performed to amplify AgeI-ORF1 fragment (Open Reading Frame, ORF; from upstream of AgeI site in ORF0 to ORF1; 204 bp) with primers of 1707KFAV4AgeIF/R ( ) using pKFAV4AP as the template (Q5 High-Fidelity DNA Polymerase); PCR was performed to amplify GFP expression cassette (1672 bp) with primers of 1707F02GFPF/R using pAd5GFP as the template; and these three fragments were fused together to generate plasmid pKFAV4APN-GFP ( pKFAV4AP N heI -GFP ) by DNA assembly. pKFAV4APN-GFP was digested with NheI, treated with CIP (Calf Intestinal Alkaline Phosphatase; New England Biolabs), and ligated with the large fragment of NheI-digested pKFAV4AP (9840 bp) to generate plasmid pKFAV4AP-GFP (16,575 bp). pKFAV4AP-GFP was digested with PacI and fused to the large fragment of AvrII-digested pKFAV4 (29,468 bp) to generate pKFAV4-GFP (45,981 bp) by DNA assembly (NEBuilderHiFi DNA Assembly Master Mix).

    Agarose Gel Electrophoresis:

    Article Title: User-Friendly Reverse Genetics System for Modification of the Right End of Fowl Adenovirus 4 Genome
    Article Snippet: Construct Intermediate Plasmid pKFAV4AP Plasmid pKFAV4 (46.2 kb) contained two AvrII sites. .. Two single-stranded oligonucleotides surrounding the AvrII sites in pKFAV4 were synthesized ( ) and fused to form a double stranded DNA (AvrII-PacI, 96 bp in length) through DNA polymerasemediated extension reaction (Q5 High-Fidelity DNA Polymerase, Cat. M0491S; New England Biolabs, Ipswich, Massachusetts, USA), and a PacI site (ttaattaa) was added in the middle to demarcate the two AvrII-centered parts. pKFAV4 was digested with AvrII, the small fragment (16.7 kb) was recovered from agarose gel (Zymoclean Large Fragment DNA Recovery Kit, Cat.D4045; Zymo Research, Irvine, CA, USA) and mixed with AvrII-PacI fragment, and Gibson assembly was performed to generate an intermediate plasmid pKFAV4AP ( pKFAV4 A vrII- P acI) (NEBuilderHiFi DNA Assembly Master Mix, Cat. E2621; New England Biolabs). .. Delete ORF1-ORF2 in pKFAV4 pKFAV4AP was digested with AgeI/NheI, and the fragment of 4942 bp was recovered; PCR was performed to amplify AgeI-ORF1 fragment (Open Reading Frame, ORF; from upstream of AgeI site in ORF0 to ORF1; 204 bp) with primers of 1707KFAV4AgeIF/R ( ) using pKFAV4AP as the template (Q5 High-Fidelity DNA Polymerase); PCR was performed to amplify GFP expression cassette (1672 bp) with primers of 1707F02GFPF/R using pAd5GFP as the template; and these three fragments were fused together to generate plasmid pKFAV4APN-GFP ( pKFAV4AP N heI -GFP ) by DNA assembly. pKFAV4APN-GFP was digested with NheI, treated with CIP (Calf Intestinal Alkaline Phosphatase; New England Biolabs), and ligated with the large fragment of NheI-digested pKFAV4AP (9840 bp) to generate plasmid pKFAV4AP-GFP (16,575 bp). pKFAV4AP-GFP was digested with PacI and fused to the large fragment of AvrII-digested pKFAV4 (29,468 bp) to generate pKFAV4-GFP (45,981 bp) by DNA assembly (NEBuilderHiFi DNA Assembly Master Mix).

    Article Title: Genomes of trombidid mites reveal novel predicted allergens and laterally transferred genes associated with secondary metabolism
    Article Snippet: .. A 0.6% Certified Megabase Agarose gel (Bio-Rad) was used to separate the fragments, and those in the range of 2–5 kb were extracted and recovered using a Zymoclean Large Fragment DNA Recovery Kit (Zymo Research). ..

    Article Title: Revealing the transcriptomic complexity of switchgrass by PacBio long-read sequencing
    Article Snippet: After the first-strand reaction, the cDNA (Additional file : Figure S18a) was amplified using a PCR primer (5′-TAGTCGAACTGAGATCTCCAGCAG-3′) and KAPA HiFi HotSart ReadyMix (KAPA). .. Two size-selection procedures were used: (1) a set of libraries was made from the cDNA generated from five cycles of PCR and gel selection, and different size fractions (cDNA < 0.5 kb, 0.5–1 kb, 1–2 kb, 2–3 kb, and 3–6 kb) were collected from a 0.8% agarose gel and purified using the Zymoclean Large Fragment DNA Recovery Kit (Zymo) (Additional file : Figure S18b); and (2) to reduce bias towards short reads, a second procedure of size selection was performed on a Sage Science Electrophoretic Lateral Fractionator (ELF), resulting in the isolation of 10 discrete size fractions. cDNA from 10 cycles of PCR was loaded onto a 0.75% cassette (Sage), and size-based separation mode was used to select cDNA from 500 bp. cDNA fractions > 5 Kbp were collected for additional 10 PCR cycles and ELF selection to enrich long reads (Additional file : Figure S18c). .. After size selection, the collected cDNA fractions were treated with DNA damage repair mix [ ], followed by end repair and ligation of SMRT adapters using the PacBio SMRTbell Template Prep Kit to create PacBio libraries.

    Article Title: Clade II Candida auris possess genomic structural variations related to an ancestral strain
    Article Snippet: .. After centrifugation at 17,400 x g for 5 min, the upper aqueous phase was subjected to electrophoresis on a 1% TAE agarose gel, followed by purification of long size DNA (15-40kb) using a Zymoclean large-fragment DNA recovery kit (Zymoresearch, Irvine, CA, USA). .. Short read and long read DNA libraries with purified DNA were constructed using QIAseq FX DNA Library Kit (QIAGEN, Hilden, Germany) and the SMRTbell template prep kit 1.0 (PacBio, Menlo Park, CA, USA) according to the manufacturer instructions, followed by whole-genome sequencing using NextSeq (Illumina, San Diego, CA, USA), MiSeq (Illumina), and Sequel (PacBio).

    Plasmid Preparation:

    Article Title: User-Friendly Reverse Genetics System for Modification of the Right End of Fowl Adenovirus 4 Genome
    Article Snippet: Construct Intermediate Plasmid pKFAV4AP Plasmid pKFAV4 (46.2 kb) contained two AvrII sites. .. Two single-stranded oligonucleotides surrounding the AvrII sites in pKFAV4 were synthesized ( ) and fused to form a double stranded DNA (AvrII-PacI, 96 bp in length) through DNA polymerasemediated extension reaction (Q5 High-Fidelity DNA Polymerase, Cat. M0491S; New England Biolabs, Ipswich, Massachusetts, USA), and a PacI site (ttaattaa) was added in the middle to demarcate the two AvrII-centered parts. pKFAV4 was digested with AvrII, the small fragment (16.7 kb) was recovered from agarose gel (Zymoclean Large Fragment DNA Recovery Kit, Cat.D4045; Zymo Research, Irvine, CA, USA) and mixed with AvrII-PacI fragment, and Gibson assembly was performed to generate an intermediate plasmid pKFAV4AP ( pKFAV4 A vrII- P acI) (NEBuilderHiFi DNA Assembly Master Mix, Cat. E2621; New England Biolabs). .. Delete ORF1-ORF2 in pKFAV4 pKFAV4AP was digested with AgeI/NheI, and the fragment of 4942 bp was recovered; PCR was performed to amplify AgeI-ORF1 fragment (Open Reading Frame, ORF; from upstream of AgeI site in ORF0 to ORF1; 204 bp) with primers of 1707KFAV4AgeIF/R ( ) using pKFAV4AP as the template (Q5 High-Fidelity DNA Polymerase); PCR was performed to amplify GFP expression cassette (1672 bp) with primers of 1707F02GFPF/R using pAd5GFP as the template; and these three fragments were fused together to generate plasmid pKFAV4APN-GFP ( pKFAV4AP N heI -GFP ) by DNA assembly. pKFAV4APN-GFP was digested with NheI, treated with CIP (Calf Intestinal Alkaline Phosphatase; New England Biolabs), and ligated with the large fragment of NheI-digested pKFAV4AP (9840 bp) to generate plasmid pKFAV4AP-GFP (16,575 bp). pKFAV4AP-GFP was digested with PacI and fused to the large fragment of AvrII-digested pKFAV4 (29,468 bp) to generate pKFAV4-GFP (45,981 bp) by DNA assembly (NEBuilderHiFi DNA Assembly Master Mix).

    Polymerase Chain Reaction:

    Article Title: Identification of genetic linkage group 1-linked sequences in Japanese eel (Anguilla japonica) by single chromosome sorting and sequencing
    Article Snippet: PCR was conducted using PrimeSTAR GXL DNA polymerase (TaKaRa, Kusatsu, Japan) in the following condition: 30 cycles of 98°C for 10 s, 60°C for 15 s, and 68°C for 8 min. .. The PCR products were electrophoresed on 1% agarose gels, extracted from the prominent bands using the Zymoclean™ Large Fragment DNA Recovery Kit (Zymo Research, Irvine, CA, USA), and utilized for FISH. .. Chromosome preparation and FISH Chromosome slides were prepared from lymphocytes as described by Fujiwara et al. [ ].

    Article Title: Revealing the transcriptomic complexity of switchgrass by PacBio long-read sequencing
    Article Snippet: After the first-strand reaction, the cDNA (Additional file : Figure S18a) was amplified using a PCR primer (5′-TAGTCGAACTGAGATCTCCAGCAG-3′) and KAPA HiFi HotSart ReadyMix (KAPA). .. Two size-selection procedures were used: (1) a set of libraries was made from the cDNA generated from five cycles of PCR and gel selection, and different size fractions (cDNA < 0.5 kb, 0.5–1 kb, 1–2 kb, 2–3 kb, and 3–6 kb) were collected from a 0.8% agarose gel and purified using the Zymoclean Large Fragment DNA Recovery Kit (Zymo) (Additional file : Figure S18b); and (2) to reduce bias towards short reads, a second procedure of size selection was performed on a Sage Science Electrophoretic Lateral Fractionator (ELF), resulting in the isolation of 10 discrete size fractions. cDNA from 10 cycles of PCR was loaded onto a 0.75% cassette (Sage), and size-based separation mode was used to select cDNA from 500 bp. cDNA fractions > 5 Kbp were collected for additional 10 PCR cycles and ELF selection to enrich long reads (Additional file : Figure S18c). .. After size selection, the collected cDNA fractions were treated with DNA damage repair mix [ ], followed by end repair and ligation of SMRT adapters using the PacBio SMRTbell Template Prep Kit to create PacBio libraries.

    Article Title: Ribavirin inhibition of cell-culture infectious hepatitis C genotype 1-3 viruses is strain-dependent.
    Article Snippet: Ribavirin remains relevant for successful treatment of chronic hepatitis C virus (HCV) infections in low-income settings, as well as for therapy of difficult-to-treat HCV patients. .. Ribavirin remains relevant for successful treatment of chronic hepatitis C virus (HCV) infections in low-income settings, as well as for therapy of difficult-to-treat HCV patients. .. Ribavirin remains relevant for successful treatment of chronic hepatitis C virus (HCV) infections in low-income settings, as well as for therapy of difficult-to-treat HCV patients.

    Fluorescence In Situ Hybridization:

    Article Title: Identification of genetic linkage group 1-linked sequences in Japanese eel (Anguilla japonica) by single chromosome sorting and sequencing
    Article Snippet: PCR was conducted using PrimeSTAR GXL DNA polymerase (TaKaRa, Kusatsu, Japan) in the following condition: 30 cycles of 98°C for 10 s, 60°C for 15 s, and 68°C for 8 min. .. The PCR products were electrophoresed on 1% agarose gels, extracted from the prominent bands using the Zymoclean™ Large Fragment DNA Recovery Kit (Zymo Research, Irvine, CA, USA), and utilized for FISH. .. Chromosome preparation and FISH Chromosome slides were prepared from lymphocytes as described by Fujiwara et al. [ ].

    Purification:

    Article Title: In vitro self-replication and multicistronic expression of large synthetic genomes
    Article Snippet: To confirm the identity of the replication products pLD1-3 by restriction pattern analysis, TTcDR samples were processed by adding 1 µL RNAse Cocktail (Thermo Fisher) and 1 mg/ml Proteinase K. After 16 h of incubation at 37 °C, the samples were loaded on a neutral 0.8% agarose gel. .. DNA products migrating at a size of ~20–30 kb were gel-extracted and purified using the Zymoclean Large DNA Fragment Extraction Kit (Zymo Research). .. Purified DNA was cut with MluI and restriction patterns visualised by neutral gel electrophoresis as described above.

    Article Title: Revealing the transcriptomic complexity of switchgrass by PacBio long-read sequencing
    Article Snippet: After the first-strand reaction, the cDNA (Additional file : Figure S18a) was amplified using a PCR primer (5′-TAGTCGAACTGAGATCTCCAGCAG-3′) and KAPA HiFi HotSart ReadyMix (KAPA). .. Two size-selection procedures were used: (1) a set of libraries was made from the cDNA generated from five cycles of PCR and gel selection, and different size fractions (cDNA < 0.5 kb, 0.5–1 kb, 1–2 kb, 2–3 kb, and 3–6 kb) were collected from a 0.8% agarose gel and purified using the Zymoclean Large Fragment DNA Recovery Kit (Zymo) (Additional file : Figure S18b); and (2) to reduce bias towards short reads, a second procedure of size selection was performed on a Sage Science Electrophoretic Lateral Fractionator (ELF), resulting in the isolation of 10 discrete size fractions. cDNA from 10 cycles of PCR was loaded onto a 0.75% cassette (Sage), and size-based separation mode was used to select cDNA from 500 bp. cDNA fractions > 5 Kbp were collected for additional 10 PCR cycles and ELF selection to enrich long reads (Additional file : Figure S18c). .. After size selection, the collected cDNA fractions were treated with DNA damage repair mix [ ], followed by end repair and ligation of SMRT adapters using the PacBio SMRTbell Template Prep Kit to create PacBio libraries.

    Article Title: Ribavirin inhibition of cell-culture infectious hepatitis C genotype 1-3 viruses is strain-dependent.
    Article Snippet: Ribavirin remains relevant for successful treatment of chronic hepatitis C virus (HCV) infections in low-income settings, as well as for therapy of difficult-to-treat HCV patients. .. Ribavirin remains relevant for successful treatment of chronic hepatitis C virus (HCV) infections in low-income settings, as well as for therapy of difficult-to-treat HCV patients. .. Ribavirin remains relevant for successful treatment of chronic hepatitis C virus (HCV) infections in low-income settings, as well as for therapy of difficult-to-treat HCV patients.

    Article Title: Clade II Candida auris possess genomic structural variations related to an ancestral strain
    Article Snippet: .. After centrifugation at 17,400 x g for 5 min, the upper aqueous phase was subjected to electrophoresis on a 1% TAE agarose gel, followed by purification of long size DNA (15-40kb) using a Zymoclean large-fragment DNA recovery kit (Zymoresearch, Irvine, CA, USA). .. Short read and long read DNA libraries with purified DNA were constructed using QIAseq FX DNA Library Kit (QIAGEN, Hilden, Germany) and the SMRTbell template prep kit 1.0 (PacBio, Menlo Park, CA, USA) according to the manufacturer instructions, followed by whole-genome sequencing using NextSeq (Illumina, San Diego, CA, USA), MiSeq (Illumina), and Sequel (PacBio).

    Generated:

    Article Title: Revealing the transcriptomic complexity of switchgrass by PacBio long-read sequencing
    Article Snippet: After the first-strand reaction, the cDNA (Additional file : Figure S18a) was amplified using a PCR primer (5′-TAGTCGAACTGAGATCTCCAGCAG-3′) and KAPA HiFi HotSart ReadyMix (KAPA). .. Two size-selection procedures were used: (1) a set of libraries was made from the cDNA generated from five cycles of PCR and gel selection, and different size fractions (cDNA < 0.5 kb, 0.5–1 kb, 1–2 kb, 2–3 kb, and 3–6 kb) were collected from a 0.8% agarose gel and purified using the Zymoclean Large Fragment DNA Recovery Kit (Zymo) (Additional file : Figure S18b); and (2) to reduce bias towards short reads, a second procedure of size selection was performed on a Sage Science Electrophoretic Lateral Fractionator (ELF), resulting in the isolation of 10 discrete size fractions. cDNA from 10 cycles of PCR was loaded onto a 0.75% cassette (Sage), and size-based separation mode was used to select cDNA from 500 bp. cDNA fractions > 5 Kbp were collected for additional 10 PCR cycles and ELF selection to enrich long reads (Additional file : Figure S18c). .. After size selection, the collected cDNA fractions were treated with DNA damage repair mix [ ], followed by end repair and ligation of SMRT adapters using the PacBio SMRTbell Template Prep Kit to create PacBio libraries.

    Selection:

    Article Title: Revealing the transcriptomic complexity of switchgrass by PacBio long-read sequencing
    Article Snippet: After the first-strand reaction, the cDNA (Additional file : Figure S18a) was amplified using a PCR primer (5′-TAGTCGAACTGAGATCTCCAGCAG-3′) and KAPA HiFi HotSart ReadyMix (KAPA). .. Two size-selection procedures were used: (1) a set of libraries was made from the cDNA generated from five cycles of PCR and gel selection, and different size fractions (cDNA < 0.5 kb, 0.5–1 kb, 1–2 kb, 2–3 kb, and 3–6 kb) were collected from a 0.8% agarose gel and purified using the Zymoclean Large Fragment DNA Recovery Kit (Zymo) (Additional file : Figure S18b); and (2) to reduce bias towards short reads, a second procedure of size selection was performed on a Sage Science Electrophoretic Lateral Fractionator (ELF), resulting in the isolation of 10 discrete size fractions. cDNA from 10 cycles of PCR was loaded onto a 0.75% cassette (Sage), and size-based separation mode was used to select cDNA from 500 bp. cDNA fractions > 5 Kbp were collected for additional 10 PCR cycles and ELF selection to enrich long reads (Additional file : Figure S18c). .. After size selection, the collected cDNA fractions were treated with DNA damage repair mix [ ], followed by end repair and ligation of SMRT adapters using the PacBio SMRTbell Template Prep Kit to create PacBio libraries.

    Isolation:

    Article Title: Revealing the transcriptomic complexity of switchgrass by PacBio long-read sequencing
    Article Snippet: After the first-strand reaction, the cDNA (Additional file : Figure S18a) was amplified using a PCR primer (5′-TAGTCGAACTGAGATCTCCAGCAG-3′) and KAPA HiFi HotSart ReadyMix (KAPA). .. Two size-selection procedures were used: (1) a set of libraries was made from the cDNA generated from five cycles of PCR and gel selection, and different size fractions (cDNA < 0.5 kb, 0.5–1 kb, 1–2 kb, 2–3 kb, and 3–6 kb) were collected from a 0.8% agarose gel and purified using the Zymoclean Large Fragment DNA Recovery Kit (Zymo) (Additional file : Figure S18b); and (2) to reduce bias towards short reads, a second procedure of size selection was performed on a Sage Science Electrophoretic Lateral Fractionator (ELF), resulting in the isolation of 10 discrete size fractions. cDNA from 10 cycles of PCR was loaded onto a 0.75% cassette (Sage), and size-based separation mode was used to select cDNA from 500 bp. cDNA fractions > 5 Kbp were collected for additional 10 PCR cycles and ELF selection to enrich long reads (Additional file : Figure S18c). .. After size selection, the collected cDNA fractions were treated with DNA damage repair mix [ ], followed by end repair and ligation of SMRT adapters using the PacBio SMRTbell Template Prep Kit to create PacBio libraries.

    Article Title: qDSB-Seq is a general method for genome-wide quantification of DNA double-strand breaks using sequencing
    Article Snippet: After ligation, the beads were washed once with TE and encapsulated DNA was initially sonicated using Covaris S220. .. Total DNA was isolated using Zymoclean™ Large Fragment DNA Recovery Kit (Zymo Research) and once again fragmented by sonication to create ~400 bp fragments. .. Labeled fragments were captured by Dynabeads MyOne C1 beads (Invitrogen), blunted, and phosphorylated using Quick Blunting Kit (NEB), then ligated to a distal adapter (both proximal and distal adapters are identical to those used in the original BLESS method ).

    Sonication:

    Article Title: qDSB-Seq is a general method for genome-wide quantification of DNA double-strand breaks using sequencing
    Article Snippet: After ligation, the beads were washed once with TE and encapsulated DNA was initially sonicated using Covaris S220. .. Total DNA was isolated using Zymoclean™ Large Fragment DNA Recovery Kit (Zymo Research) and once again fragmented by sonication to create ~400 bp fragments. .. Labeled fragments were captured by Dynabeads MyOne C1 beads (Invitrogen), blunted, and phosphorylated using Quick Blunting Kit (NEB), then ligated to a distal adapter (both proximal and distal adapters are identical to those used in the original BLESS method ).

    Centrifugation:

    Article Title: Clade II Candida auris possess genomic structural variations related to an ancestral strain
    Article Snippet: .. After centrifugation at 17,400 x g for 5 min, the upper aqueous phase was subjected to electrophoresis on a 1% TAE agarose gel, followed by purification of long size DNA (15-40kb) using a Zymoclean large-fragment DNA recovery kit (Zymoresearch, Irvine, CA, USA). .. Short read and long read DNA libraries with purified DNA were constructed using QIAseq FX DNA Library Kit (QIAGEN, Hilden, Germany) and the SMRTbell template prep kit 1.0 (PacBio, Menlo Park, CA, USA) according to the manufacturer instructions, followed by whole-genome sequencing using NextSeq (Illumina, San Diego, CA, USA), MiSeq (Illumina), and Sequel (PacBio).

    Electrophoresis:

    Article Title: Clade II Candida auris possess genomic structural variations related to an ancestral strain
    Article Snippet: .. After centrifugation at 17,400 x g for 5 min, the upper aqueous phase was subjected to electrophoresis on a 1% TAE agarose gel, followed by purification of long size DNA (15-40kb) using a Zymoclean large-fragment DNA recovery kit (Zymoresearch, Irvine, CA, USA). .. Short read and long read DNA libraries with purified DNA were constructed using QIAseq FX DNA Library Kit (QIAGEN, Hilden, Germany) and the SMRTbell template prep kit 1.0 (PacBio, Menlo Park, CA, USA) according to the manufacturer instructions, followed by whole-genome sequencing using NextSeq (Illumina, San Diego, CA, USA), MiSeq (Illumina), and Sequel (PacBio).

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    • zymoclean large fragment dna recovery kit  (Zymo Research)
    99
    Zymo Research zymoclean large fragment dna recovery kit
    Zymoclean Large Fragment Dna Recovery Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zymoclean large fragment dna recovery kit/product/Zymo Research
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zymoclean large fragment dna recovery kit - by Bioz Stars, 2021-03
    99/100 stars
    		  Buy from Supplier

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