Structured Review

Vitrolife zona pellucida
Early PNT promotes survival of normally fertilized zygotes following PNT. a , Progression from MII arrest to completion of the 1st mitosis showing timings of early PNT (ePNT) and late PNT (LtPNT) b , Images show the steps involved in ePNT (i) Arrowheads indicate the pronuclei (PN) (ii) Enucleation pipette inserted through a laser-induced opening in the zona <t>pellucida</t> (arrow, bottom panel). Lower panel, enucleated zygote (cytoplast). Inset shows two karyoplasts, each consisting of a single PN surrounded by a small amount of cytoplasm. (iii) Karyoplasts treated with HVJ-E and inserted under the zona pellucida. Lower panel, arrow indicates removal of excess cytoplasm (see Video 2 ). c , Survival of reconstituted ePNT and LtPNT zygotes ( P
Zona Pellucida, supplied by Vitrolife, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease"

Article Title: Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease

Journal: Nature

doi: 10.1038/nature18303

Early PNT promotes survival of normally fertilized zygotes following PNT. a , Progression from MII arrest to completion of the 1st mitosis showing timings of early PNT (ePNT) and late PNT (LtPNT) b , Images show the steps involved in ePNT (i) Arrowheads indicate the pronuclei (PN) (ii) Enucleation pipette inserted through a laser-induced opening in the zona pellucida (arrow, bottom panel). Lower panel, enucleated zygote (cytoplast). Inset shows two karyoplasts, each consisting of a single PN surrounded by a small amount of cytoplasm. (iii) Karyoplasts treated with HVJ-E and inserted under the zona pellucida. Lower panel, arrow indicates removal of excess cytoplasm (see Video 2 ). c , Survival of reconstituted ePNT and LtPNT zygotes ( P
Figure Legend Snippet: Early PNT promotes survival of normally fertilized zygotes following PNT. a , Progression from MII arrest to completion of the 1st mitosis showing timings of early PNT (ePNT) and late PNT (LtPNT) b , Images show the steps involved in ePNT (i) Arrowheads indicate the pronuclei (PN) (ii) Enucleation pipette inserted through a laser-induced opening in the zona pellucida (arrow, bottom panel). Lower panel, enucleated zygote (cytoplast). Inset shows two karyoplasts, each consisting of a single PN surrounded by a small amount of cytoplasm. (iii) Karyoplasts treated with HVJ-E and inserted under the zona pellucida. Lower panel, arrow indicates removal of excess cytoplasm (see Video 2 ). c , Survival of reconstituted ePNT and LtPNT zygotes ( P

Techniques Used: Transferring

Related Articles

Polymerase Chain Reaction:

Article Title: mtDNA dynamics between cleavage-stage embryos and blastocysts
Article Snippet: .. Cleavage-stage embryos were placed in a droplet containing Ca2+ /Mg2+-free medium (G-PGD, Vitrolife, Göteborg, Sweden/LifeGlobal, Guilford, CT), the zona pellucida was perforated by laser pulses (OCTAX, Herborn, Germany) and a single blastomere was withdrawn from each embryo and placed in 0.2 mL of PCR tubes containing 2 μL PBS. ..

Incubation:

Article Title: Bisphenol A affects early bovine embryo development and metabolism that is negated by an oestrogen receptor inhibitor
Article Snippet: Differential cell staining The number of cells in the inner cell mass (ICM) and the trophectoderm (TE) of expanded and hatched blastocysts was determined using differential staining on day 7.5 post-IVF, based on previously published methods . .. Briefly, embryos were placed in 0.5% (w/v; 10 μL) pronase in GMOPS (Vitrolife) to remove the zona pellucida, and then washed in GMOPS-PLUS (Vitrolife) for 5 min. Embryos were subsequently incubated in 10 μL of 10 mM trinitrobenzenesulfonic acid (TNBS) in 4 mg/mL polyvinyl pyrollidone (PVP) in simple-G1 medium (Vitrolife; G1-PVP) for 20 min, washed twice in GMOPS-PLUS then transferred to 0.1 mg/mL anti-2,4 dinitrophenol-G1-PVP (10 μL) for 10 min. ..

Transferring:

Article Title: Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease
Article Snippet: The inner diameter pipette measurements were 25-35µm for LtPNT, and 17µm for ePNT. (3) The PN, surrounded by a small amount of cytoplasm, were aspirated into the enucleation pipette, either as a single karyoplast, or as two separate karyoplasts (see and ). (4) Karyoplasts were briefly exposed to a suspension of HVJ-E; GenomONE-CF Ex (Cosmo Bio, Japan). .. Undiluted suspension was used for LtPNT and ePNT (Series I) and a 1:10 dilution was used for ePNT (Series II). (5) The pipette containing the karyoplasts was inserted through the laser-drilled opening in the zona pellucida and karyoplasts were gently expelled into the perivitelline space and allowed to fuse with the cytoplast. (6) Reconstituted and control zygotes were cultured either in a sequential medium G-1 Plus (day 1-3)/ G-2 Plus (day 3-6) (Vitrolife; LtPNT and ePNT (series I)), or in the single-step G-TL medium (Vitrolife; ePNT (series II)) from day 1-6. .. Time-lapse embryo imaging was performed for three sets of ePNT (Series II) experiments using the Primo Vision Time-lapse monitoring system (Vitrolife, Sweden).

Cell Culture:

Article Title: Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease
Article Snippet: The inner diameter pipette measurements were 25-35µm for LtPNT, and 17µm for ePNT. (3) The PN, surrounded by a small amount of cytoplasm, were aspirated into the enucleation pipette, either as a single karyoplast, or as two separate karyoplasts (see and ). (4) Karyoplasts were briefly exposed to a suspension of HVJ-E; GenomONE-CF Ex (Cosmo Bio, Japan). .. Undiluted suspension was used for LtPNT and ePNT (Series I) and a 1:10 dilution was used for ePNT (Series II). (5) The pipette containing the karyoplasts was inserted through the laser-drilled opening in the zona pellucida and karyoplasts were gently expelled into the perivitelline space and allowed to fuse with the cytoplast. (6) Reconstituted and control zygotes were cultured either in a sequential medium G-1 Plus (day 1-3)/ G-2 Plus (day 3-6) (Vitrolife; LtPNT and ePNT (series I)), or in the single-step G-TL medium (Vitrolife; ePNT (series II)) from day 1-6. .. Time-lapse embryo imaging was performed for three sets of ePNT (Series II) experiments using the Primo Vision Time-lapse monitoring system (Vitrolife, Sweden).

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    Vitrolife zona pellucida
    Zona Pellucida, supplied by Vitrolife, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zona pellucida/product/Vitrolife
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zona pellucida - by Bioz Stars, 2021-04
    86/100 stars
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