zona pellucida (Vitrolife)
86
Structured Review
Vitrolife
zona pellucida
Zona Pellucida, supplied by Vitrolife, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zona pellucida/product/Vitrolife
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Zona Pellucida, supplied by Vitrolife, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zona pellucida/product/Vitrolife
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
zona pellucida - by Bioz Stars,
2021-04
86/100 stars
Images
1) Product Images from "Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease"
Article Title: Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease
Journal: Nature
doi: 10.1038/nature18303
Figure Legend Snippet: Early PNT promotes survival of normally fertilized zygotes following PNT. a , Progression from MII arrest to completion of the 1st mitosis showing timings of early PNT (ePNT) and late PNT (LtPNT) b , Images show the steps involved in ePNT (i) Arrowheads indicate the pronuclei (PN) (ii) Enucleation pipette inserted through a laser-induced opening in the zona pellucida (arrow, bottom panel). Lower panel, enucleated zygote (cytoplast). Inset shows two karyoplasts, each consisting of a single PN surrounded by a small amount of cytoplasm. (iii) Karyoplasts treated with HVJ-E and inserted under the zona pellucida. Lower panel, arrow indicates removal of excess cytoplasm (see Video 2 ). c , Survival of reconstituted ePNT and LtPNT zygotes ( P
Techniques Used: Transferring
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Polymerase Chain Reaction:Article Title: mtDNA dynamics between cleavage-stage embryos and blastocysts Article Snippet: .. Incubation:Article Title: Bisphenol A affects early bovine embryo development and metabolism that is negated by an oestrogen receptor inhibitor Article Snippet: Differential cell staining The number of cells in the inner cell mass (ICM) and the trophectoderm (TE) of expanded and hatched blastocysts was determined using differential staining on day 7.5 post-IVF, based on previously published methods . .. Briefly, embryos were placed in 0.5% (w/v; 10 μL) pronase in GMOPS (Vitrolife) to remove the Transferring:Article Title: Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease Article Snippet: The inner diameter pipette measurements were 25-35µm for LtPNT, and 17µm for ePNT. (3) The PN, surrounded by a small amount of cytoplasm, were aspirated into the enucleation pipette, either as a single karyoplast, or as two separate karyoplasts (see and ). (4) Karyoplasts were briefly exposed to a suspension of HVJ-E; GenomONE-CF Ex (Cosmo Bio, Japan). .. Undiluted suspension was used for LtPNT and ePNT (Series I) and a 1:10 dilution was used for ePNT (Series II). (5) The pipette containing the karyoplasts was inserted through the laser-drilled opening in the Cell Culture:Article Title: Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease Article Snippet: The inner diameter pipette measurements were 25-35µm for LtPNT, and 17µm for ePNT. (3) The PN, surrounded by a small amount of cytoplasm, were aspirated into the enucleation pipette, either as a single karyoplast, or as two separate karyoplasts (see and ). (4) Karyoplasts were briefly exposed to a suspension of HVJ-E; GenomONE-CF Ex (Cosmo Bio, Japan). .. Undiluted suspension was used for LtPNT and ePNT (Series I) and a 1:10 dilution was used for ePNT (Series II). (5) The pipette containing the karyoplasts was inserted through the laser-drilled opening in the |