zona pellucida free  (Millipore)


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    Structured Review

    Millipore zona pellucida free
    Spermiograms and in vitro fertilization assays. Spermiograms from PCI +/+ ( a ) and from PCI +/– ( b ) were normal, whereas in spermiograms from PCI –/– mice ( c ) immature and malformed cells were prevalent. ( d ) In in vitro fertilization assays, sperm obtained from PCI +/– mice were able to bind and subsequently fertilize PCI +/+ oocytes. Sperm obtained from PCI –/– males failed to fertilize zona <t>pellucida–containing</t> ( e ) or zona pellucida–free ( f ) oocytes.
    Zona Pellucida Free, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zona pellucida free/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zona pellucida free - by Bioz Stars, 2020-04
    93/100 stars

    Images

    1) Product Images from "Disruption of the protein C inhibitor gene results in impaired spermatogenesis and male infertility"

    Article Title: Disruption of the protein C inhibitor gene results in impaired spermatogenesis and male infertility

    Journal: The Journal of Clinical Investigation

    doi:

    Spermiograms and in vitro fertilization assays. Spermiograms from PCI +/+ ( a ) and from PCI +/– ( b ) were normal, whereas in spermiograms from PCI –/– mice ( c ) immature and malformed cells were prevalent. ( d ) In in vitro fertilization assays, sperm obtained from PCI +/– mice were able to bind and subsequently fertilize PCI +/+ oocytes. Sperm obtained from PCI –/– males failed to fertilize zona pellucida–containing ( e ) or zona pellucida–free ( f ) oocytes.
    Figure Legend Snippet: Spermiograms and in vitro fertilization assays. Spermiograms from PCI +/+ ( a ) and from PCI +/– ( b ) were normal, whereas in spermiograms from PCI –/– mice ( c ) immature and malformed cells were prevalent. ( d ) In in vitro fertilization assays, sperm obtained from PCI +/– mice were able to bind and subsequently fertilize PCI +/+ oocytes. Sperm obtained from PCI –/– males failed to fertilize zona pellucida–containing ( e ) or zona pellucida–free ( f ) oocytes.

    Techniques Used: In Vitro, Mouse Assay

    Related Articles

    Microscopy:

    Article Title: An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells
    Article Snippet: After injected embryos reformed blastocoel, the chimera embryos were live cell imaged using Leica microscope fitted with a live cell imaging system and fixed after 24–36 h post-injection for staining and confocal imaging. .. For embryo immunostaining, zona pellucida-free injected embryos were fixed with 3.5% paraformaldehyde, permeabilized in 0.5% Triton X-100 (Sigma) and blocked with 5% BSA and then incubated with primary antibodies against CDX2 (BioGenex), β-Catenin (1:50, Abcam) and detected by DyLight 549- or 633- conjugated secondary antibodies(Thermo).

    In Vitro:

    Article Title: Disruption of the protein C inhibitor gene results in impaired spermatogenesis and male infertility
    Article Snippet: Paragraph title: In vitro fertilization test and sperm-egg binding assay. ... These oocytes were either surrounded by cumulus cells, cumulus cell–free (removed by treatment with 0.1% hyaluronidase), or zona pellucida–free (removed by treatment with 0.1% proteinase K; Sigma-Aldrich).

    Article Title: An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells
    Article Snippet: Paragraph title: Mouse blastocyst injection and in vitro culture ... For embryo immunostaining, zona pellucida-free injected embryos were fixed with 3.5% paraformaldehyde, permeabilized in 0.5% Triton X-100 (Sigma) and blocked with 5% BSA and then incubated with primary antibodies against CDX2 (BioGenex), β-Catenin (1:50, Abcam) and detected by DyLight 549- or 633- conjugated secondary antibodies(Thermo).

    Fluorescence:

    Article Title: An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells
    Article Snippet: For embryo immunostaining, zona pellucida-free injected embryos were fixed with 3.5% paraformaldehyde, permeabilized in 0.5% Triton X-100 (Sigma) and blocked with 5% BSA and then incubated with primary antibodies against CDX2 (BioGenex), β-Catenin (1:50, Abcam) and detected by DyLight 549- or 633- conjugated secondary antibodies(Thermo). .. A Nikon-A1 fluorescence microscope was used for image acquisition.

    Live Cell Imaging:

    Article Title: An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells
    Article Snippet: After injected embryos reformed blastocoel, the chimera embryos were live cell imaged using Leica microscope fitted with a live cell imaging system and fixed after 24–36 h post-injection for staining and confocal imaging. .. For embryo immunostaining, zona pellucida-free injected embryos were fixed with 3.5% paraformaldehyde, permeabilized in 0.5% Triton X-100 (Sigma) and blocked with 5% BSA and then incubated with primary antibodies against CDX2 (BioGenex), β-Catenin (1:50, Abcam) and detected by DyLight 549- or 633- conjugated secondary antibodies(Thermo).

    Immunostaining:

    Article Title: An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells
    Article Snippet: .. For embryo immunostaining, zona pellucida-free injected embryos were fixed with 3.5% paraformaldehyde, permeabilized in 0.5% Triton X-100 (Sigma) and blocked with 5% BSA and then incubated with primary antibodies against CDX2 (BioGenex), β-Catenin (1:50, Abcam) and detected by DyLight 549- or 633- conjugated secondary antibodies(Thermo). .. Nuclei were stained with DAPI (Sigma).

    Light Microscopy:

    Article Title: Disruption of the protein C inhibitor gene results in impaired spermatogenesis and male infertility
    Article Snippet: These oocytes were either surrounded by cumulus cells, cumulus cell–free (removed by treatment with 0.1% hyaluronidase), or zona pellucida–free (removed by treatment with 0.1% proteinase K; Sigma-Aldrich). .. Sperm number and motility (of at least 200 sperm) were determined by light microscopy.

    Incubation:

    Article Title: An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells
    Article Snippet: .. For embryo immunostaining, zona pellucida-free injected embryos were fixed with 3.5% paraformaldehyde, permeabilized in 0.5% Triton X-100 (Sigma) and blocked with 5% BSA and then incubated with primary antibodies against CDX2 (BioGenex), β-Catenin (1:50, Abcam) and detected by DyLight 549- or 633- conjugated secondary antibodies(Thermo). .. Nuclei were stained with DAPI (Sigma).

    Cell Culture:

    Article Title: An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells
    Article Snippet: After injection, embryos containing hESCs were cultured in medium supplemented with naïve culture medium:KOSM (1:1) (Chen et al., ) in 37°C, 5% CO2 incubator. .. For embryo immunostaining, zona pellucida-free injected embryos were fixed with 3.5% paraformaldehyde, permeabilized in 0.5% Triton X-100 (Sigma) and blocked with 5% BSA and then incubated with primary antibodies against CDX2 (BioGenex), β-Catenin (1:50, Abcam) and detected by DyLight 549- or 633- conjugated secondary antibodies(Thermo).

    Staining:

    Article Title: An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells
    Article Snippet: After injected embryos reformed blastocoel, the chimera embryos were live cell imaged using Leica microscope fitted with a live cell imaging system and fixed after 24–36 h post-injection for staining and confocal imaging. .. For embryo immunostaining, zona pellucida-free injected embryos were fixed with 3.5% paraformaldehyde, permeabilized in 0.5% Triton X-100 (Sigma) and blocked with 5% BSA and then incubated with primary antibodies against CDX2 (BioGenex), β-Catenin (1:50, Abcam) and detected by DyLight 549- or 633- conjugated secondary antibodies(Thermo).

    Injection:

    Article Title: An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells
    Article Snippet: .. For embryo immunostaining, zona pellucida-free injected embryos were fixed with 3.5% paraformaldehyde, permeabilized in 0.5% Triton X-100 (Sigma) and blocked with 5% BSA and then incubated with primary antibodies against CDX2 (BioGenex), β-Catenin (1:50, Abcam) and detected by DyLight 549- or 633- conjugated secondary antibodies(Thermo). .. Nuclei were stained with DAPI (Sigma).

    Binding Assay:

    Article Title: Disruption of the protein C inhibitor gene results in impaired spermatogenesis and male infertility
    Article Snippet: Paragraph title: In vitro fertilization test and sperm-egg binding assay. ... These oocytes were either surrounded by cumulus cells, cumulus cell–free (removed by treatment with 0.1% hyaluronidase), or zona pellucida–free (removed by treatment with 0.1% proteinase K; Sigma-Aldrich).

    Imaging:

    Article Title: An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells
    Article Snippet: After injected embryos reformed blastocoel, the chimera embryos were live cell imaged using Leica microscope fitted with a live cell imaging system and fixed after 24–36 h post-injection for staining and confocal imaging. .. For embryo immunostaining, zona pellucida-free injected embryos were fixed with 3.5% paraformaldehyde, permeabilized in 0.5% Triton X-100 (Sigma) and blocked with 5% BSA and then incubated with primary antibodies against CDX2 (BioGenex), β-Catenin (1:50, Abcam) and detected by DyLight 549- or 633- conjugated secondary antibodies(Thermo).

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  • 90
    Millipore zona pellucida free eggs
    Zona Pellucida Free Eggs, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zona pellucida free eggs/product/Millipore
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zona pellucida free eggs - by Bioz Stars, 2020-04
    90/100 stars
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