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Thermo Fisher zncl2
Zncl2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zncl2/product/Thermo Fisher
Average 92 stars, based on 10 article reviews
Price from $9.99 to $1999.99
zncl2 - by Bioz Stars, 2020-05
92/100 stars

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Amplification:

Article Title: Decoding bacteriophage P22 assembly: identification of two charged residues in scaffolding protein responsible for coat protein interaction
Article Snippet: .. This amplified DNA was used to electroporate UB-1751 or UB-1838 cells, and transformants were selected for loss of the TetRA cassette on plates containing 24 mg/ml fusaric acid (Sigma, St. Louis, MO), 20 mM ZnCl2 and 0.25 mg/ml anhydrotetracycline hydrochloride (Thermo Fisher Scientific, Waltham, MA) while retaining kanamycin resistance. .. The loss of tetracycline resistance and presence of prophage mutations were confirmed by sequencing using either whole phage DNA (when the phage was fully functional) or relevant PCR products amplified from nonfunctional prophages as template; the entire scaffolding protein gene was sequenced in each of the mutant phages to ensure no additional scaffolding protein mutations were present.

Fluorescence:

Article Title: Zn(II)-Coordinated Quantum Dot-FRET Nanosensors for the Detection of Protein Kinase Activity
Article Snippet: .. Afterward, QD525 (20 µL at 10 nM) and ZnCl2 (1 µL at 10 mM) were added to the solution containing peptide-conjugated SA beads to give a final volume of 100 µL, which was followed by 5-min incubation at RT and fluorescence measurement at wavelengths of 450–650 nm using a microplate reader (VarioskanTM Flash, Thermo Scientific, Waltham, MA, USA). .. Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) Analysis A C18 pipette-tip was used to concentrate and desalt the peptide substrates with or without kinase reaction according to the manufacturer’s specifications.

Protease Inhibitor:

Article Title: The KRAB zinc finger protein ZFP809 is required to initiate epigenetic silencing of endogenous retroviruses
Article Snippet: .. EMSA binding reactions were carried out with nuclear extracts from ∼106 cells per reaction in modified Thornell binding buffer (25 mM HEPES at pH 7.9, 1 mM EDTA, 12.5% [v/v] glycerol, 5 mM DTT, 25 ng of poly(dI-dC) per microliter, 150 mM NaCl, 5 mM KCl, 3 mM MgCl2 , 0.1 mM ZnCl2 , 15% fetal calf serum, 1× protease inhibitor mix [Invitrogen]) for 30 min at 30°C and analyzed by electrophoresis on 4% native polyacrylamide gels. .. Differential gene expression analysis was performed by counting reads overlapping with exons of RefSeq genes by BEDTools.

Electrophoresis:

Article Title: The KRAB zinc finger protein ZFP809 is required to initiate epigenetic silencing of endogenous retroviruses
Article Snippet: .. EMSA binding reactions were carried out with nuclear extracts from ∼106 cells per reaction in modified Thornell binding buffer (25 mM HEPES at pH 7.9, 1 mM EDTA, 12.5% [v/v] glycerol, 5 mM DTT, 25 ng of poly(dI-dC) per microliter, 150 mM NaCl, 5 mM KCl, 3 mM MgCl2 , 0.1 mM ZnCl2 , 15% fetal calf serum, 1× protease inhibitor mix [Invitrogen]) for 30 min at 30°C and analyzed by electrophoresis on 4% native polyacrylamide gels. .. Differential gene expression analysis was performed by counting reads overlapping with exons of RefSeq genes by BEDTools.

Incubation:

Article Title: YY1 tethers Xist RNA to the inactive X nucleation center
Article Snippet: .. 5 μg of total RNA was incubated with protein-bead complexes at room temperature for 1 h in PBS containing 2 mM MgCl2 , 0.2 mM ZnCl2 , 15 mM β-mercaptoethanol, 100 U/ml RNase Inhibitor, 0.1 mg/ml yeast tRNA (Ambion), 0.05% BSA and 0.2% NP40. ..

Article Title: Zn(II)-Coordinated Quantum Dot-FRET Nanosensors for the Detection of Protein Kinase Activity
Article Snippet: .. Afterward, QD525 (20 µL at 10 nM) and ZnCl2 (1 µL at 10 mM) were added to the solution containing peptide-conjugated SA beads to give a final volume of 100 µL, which was followed by 5-min incubation at RT and fluorescence measurement at wavelengths of 450–650 nm using a microplate reader (VarioskanTM Flash, Thermo Scientific, Waltham, MA, USA). .. Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) Analysis A C18 pipette-tip was used to concentrate and desalt the peptide substrates with or without kinase reaction according to the manufacturer’s specifications.

other:

Article Title: Characterization of Biochemical Properties of Bacillus subtilis RecQ Helicase
Article Snippet: Puratronic CaCl2 , MgCl2 , MnCl2 , NiCl2 , and ZnCl2 were from Alfa Aesar (Ward Hill, MA).

De-Phosphorylation Assay:

Article Title: Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences
Article Snippet: .. Plasmid DNA was hydrolyzed with nuclease P1 (Sigma; 2 U) in 30 mM sodium acetate, pH 5.3, 0.5 mM ZnCl2 in a 100 μL volume at 50 °C for 2 h. Subsequent dephosphorylation was carried out by addition of 10 μL of 1 M Tris-Cl, pH 8.0, and 5 U of alkaline phosphatase (Fermentas, FASTAP) at 37 °C for another 2 h. The enzymes were subsequently removed by ultrafiltration (AMICON ULTRA 0.5 mL Ultracel 3 kD) followed by addition of 100 pmol of d(Gps A) S p as an internal standard. ..

Modification:

Article Title: The KRAB zinc finger protein ZFP809 is required to initiate epigenetic silencing of endogenous retroviruses
Article Snippet: .. EMSA binding reactions were carried out with nuclear extracts from ∼106 cells per reaction in modified Thornell binding buffer (25 mM HEPES at pH 7.9, 1 mM EDTA, 12.5% [v/v] glycerol, 5 mM DTT, 25 ng of poly(dI-dC) per microliter, 150 mM NaCl, 5 mM KCl, 3 mM MgCl2 , 0.1 mM ZnCl2 , 15% fetal calf serum, 1× protease inhibitor mix [Invitrogen]) for 30 min at 30°C and analyzed by electrophoresis on 4% native polyacrylamide gels. .. Differential gene expression analysis was performed by counting reads overlapping with exons of RefSeq genes by BEDTools.

Binding Assay:

Article Title: The KRAB zinc finger protein ZFP809 is required to initiate epigenetic silencing of endogenous retroviruses
Article Snippet: .. EMSA binding reactions were carried out with nuclear extracts from ∼106 cells per reaction in modified Thornell binding buffer (25 mM HEPES at pH 7.9, 1 mM EDTA, 12.5% [v/v] glycerol, 5 mM DTT, 25 ng of poly(dI-dC) per microliter, 150 mM NaCl, 5 mM KCl, 3 mM MgCl2 , 0.1 mM ZnCl2 , 15% fetal calf serum, 1× protease inhibitor mix [Invitrogen]) for 30 min at 30°C and analyzed by electrophoresis on 4% native polyacrylamide gels. .. Differential gene expression analysis was performed by counting reads overlapping with exons of RefSeq genes by BEDTools.

Plasmid Preparation:

Article Title: Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences
Article Snippet: .. Plasmid DNA was hydrolyzed with nuclease P1 (Sigma; 2 U) in 30 mM sodium acetate, pH 5.3, 0.5 mM ZnCl2 in a 100 μL volume at 50 °C for 2 h. Subsequent dephosphorylation was carried out by addition of 10 μL of 1 M Tris-Cl, pH 8.0, and 5 U of alkaline phosphatase (Fermentas, FASTAP) at 37 °C for another 2 h. The enzymes were subsequently removed by ultrafiltration (AMICON ULTRA 0.5 mL Ultracel 3 kD) followed by addition of 100 pmol of d(Gps A) S p as an internal standard. ..

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    Thermo Fisher zncl2
    Zncl2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zncl2/product/Thermo Fisher
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    zncl2 - by Bioz Stars, 2020-05
    92/100 stars
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