Structured Review

The Nest Group zic philic hplc column
Zic Philic Hplc Column, supplied by The Nest Group, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zic philic hplc column/product/The Nest Group
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
zic philic hplc column - by Bioz Stars, 2021-07
86/100 stars

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Concentration Assay:

Article Title: Persulfide Reactivity in the Detection of Protein S-Sulfhydration
Article Snippet: The resulting protein (100 µL, 62 µM) was then incubated with a Na2 S solution (1 µL, 30 mM) at rt for 10 min, and purified by spin column to give the Gpx3 persulfide, which was immediately used in downstream reactions. .. Freshly prepared GSH or protein persulfide (final concentration: 1 mM for glutathione; 20 µM for protein) was incubated with IAM or other nucleophilic or electrophilic reagents (final concentration: 4 mM for glutathione; 2 mM for protein) at rt for 1 h. After purification, aliquots of the samples were analyzed by electrospray LC/MS on Poroshell 120 (Agilent) or ZIC® -pHILIC HPLC column (the Nest group). .. To a solution of freshly prepared glutathione persulfide (1 mM final concentration) in 1 mL Tris-HCl buffer (100 mM, pH 7.4), was added MMTS or BBMTS (4 mM final concentration), and the solution was stirred at rt for 30 min.

Incubation:

Article Title: Persulfide Reactivity in the Detection of Protein S-Sulfhydration
Article Snippet: The resulting protein (100 µL, 62 µM) was then incubated with a Na2 S solution (1 µL, 30 mM) at rt for 10 min, and purified by spin column to give the Gpx3 persulfide, which was immediately used in downstream reactions. .. Freshly prepared GSH or protein persulfide (final concentration: 1 mM for glutathione; 20 µM for protein) was incubated with IAM or other nucleophilic or electrophilic reagents (final concentration: 4 mM for glutathione; 2 mM for protein) at rt for 1 h. After purification, aliquots of the samples were analyzed by electrospray LC/MS on Poroshell 120 (Agilent) or ZIC® -pHILIC HPLC column (the Nest group). .. To a solution of freshly prepared glutathione persulfide (1 mM final concentration) in 1 mL Tris-HCl buffer (100 mM, pH 7.4), was added MMTS or BBMTS (4 mM final concentration), and the solution was stirred at rt for 30 min.

Purification:

Article Title: Persulfide Reactivity in the Detection of Protein S-Sulfhydration
Article Snippet: The resulting protein (100 µL, 62 µM) was then incubated with a Na2 S solution (1 µL, 30 mM) at rt for 10 min, and purified by spin column to give the Gpx3 persulfide, which was immediately used in downstream reactions. .. Freshly prepared GSH or protein persulfide (final concentration: 1 mM for glutathione; 20 µM for protein) was incubated with IAM or other nucleophilic or electrophilic reagents (final concentration: 4 mM for glutathione; 2 mM for protein) at rt for 1 h. After purification, aliquots of the samples were analyzed by electrospray LC/MS on Poroshell 120 (Agilent) or ZIC® -pHILIC HPLC column (the Nest group). .. To a solution of freshly prepared glutathione persulfide (1 mM final concentration) in 1 mL Tris-HCl buffer (100 mM, pH 7.4), was added MMTS or BBMTS (4 mM final concentration), and the solution was stirred at rt for 30 min.

Liquid Chromatography with Mass Spectroscopy:

Article Title: Persulfide Reactivity in the Detection of Protein S-Sulfhydration
Article Snippet: The resulting protein (100 µL, 62 µM) was then incubated with a Na2 S solution (1 µL, 30 mM) at rt for 10 min, and purified by spin column to give the Gpx3 persulfide, which was immediately used in downstream reactions. .. Freshly prepared GSH or protein persulfide (final concentration: 1 mM for glutathione; 20 µM for protein) was incubated with IAM or other nucleophilic or electrophilic reagents (final concentration: 4 mM for glutathione; 2 mM for protein) at rt for 1 h. After purification, aliquots of the samples were analyzed by electrospray LC/MS on Poroshell 120 (Agilent) or ZIC® -pHILIC HPLC column (the Nest group). .. To a solution of freshly prepared glutathione persulfide (1 mM final concentration) in 1 mL Tris-HCl buffer (100 mM, pH 7.4), was added MMTS or BBMTS (4 mM final concentration), and the solution was stirred at rt for 30 min.

High Performance Liquid Chromatography:

Article Title: Persulfide Reactivity in the Detection of Protein S-Sulfhydration
Article Snippet: The resulting protein (100 µL, 62 µM) was then incubated with a Na2 S solution (1 µL, 30 mM) at rt for 10 min, and purified by spin column to give the Gpx3 persulfide, which was immediately used in downstream reactions. .. Freshly prepared GSH or protein persulfide (final concentration: 1 mM for glutathione; 20 µM for protein) was incubated with IAM or other nucleophilic or electrophilic reagents (final concentration: 4 mM for glutathione; 2 mM for protein) at rt for 1 h. After purification, aliquots of the samples were analyzed by electrospray LC/MS on Poroshell 120 (Agilent) or ZIC® -pHILIC HPLC column (the Nest group). .. To a solution of freshly prepared glutathione persulfide (1 mM final concentration) in 1 mL Tris-HCl buffer (100 mM, pH 7.4), was added MMTS or BBMTS (4 mM final concentration), and the solution was stirred at rt for 30 min.

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    The Nest Group zic philic hplc column
    Zic Philic Hplc Column, supplied by The Nest Group, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zic philic hplc column/product/The Nest Group
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zic philic hplc column - by Bioz Stars, 2021-07
    86/100 stars
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