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Merck & Co zic philic column
Analysis of metabolic flux of glutamine by 13 C 5 -L-glutamine supplementation and subsequent LC-MS analysis. ( A ) Relative abundance of GSH, GSSG and TCA cycle metabolites was determined by using LC-MS analysis with <t>ZIC-pHILIC</t> chromatography. Data are presented as a mean of n = 3 ± SEM. Unpaired t test. ( B ) mRNA expression and protein concentration analysis of the acetyl-CoA-carboxylase (ACC). Basal ACC1 and ACC2 mRNA expression is determined by qRT-PCR and is related to the housekeeping gene RPL37A. Data are presented as a mean of n = 3 ± SEM. Unpaired t test with Welch’s correction. The ACC phospho/ACC total ratio protein concentration was determined by Western blot analysis using an anti-ACC total and anti-phospho-ACC antibody and is related to the housekeeper protein Hsp90. A representative blot is displayed. Data are presented as mean of n = 5 ± SEM. *p ≤ 0 . 05 , **p ≤ 0 . 01,***p ≤ 0.001 .
Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zic philic column/product/Merck & Co
Average 86 stars, based on 1 article reviews
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zic philic column - by Bioz Stars, 2021-06
86/100 stars

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1) Product Images from "UGCG influences glutamine metabolism of breast cancer cells"

Article Title: UGCG influences glutamine metabolism of breast cancer cells

Journal: Scientific Reports

doi: 10.1038/s41598-019-52169-7

Analysis of metabolic flux of glutamine by 13 C 5 -L-glutamine supplementation and subsequent LC-MS analysis. ( A ) Relative abundance of GSH, GSSG and TCA cycle metabolites was determined by using LC-MS analysis with ZIC-pHILIC chromatography. Data are presented as a mean of n = 3 ± SEM. Unpaired t test. ( B ) mRNA expression and protein concentration analysis of the acetyl-CoA-carboxylase (ACC). Basal ACC1 and ACC2 mRNA expression is determined by qRT-PCR and is related to the housekeeping gene RPL37A. Data are presented as a mean of n = 3 ± SEM. Unpaired t test with Welch’s correction. The ACC phospho/ACC total ratio protein concentration was determined by Western blot analysis using an anti-ACC total and anti-phospho-ACC antibody and is related to the housekeeper protein Hsp90. A representative blot is displayed. Data are presented as mean of n = 5 ± SEM. *p ≤ 0 . 05 , **p ≤ 0 . 01,***p ≤ 0.001 .
Figure Legend Snippet: Analysis of metabolic flux of glutamine by 13 C 5 -L-glutamine supplementation and subsequent LC-MS analysis. ( A ) Relative abundance of GSH, GSSG and TCA cycle metabolites was determined by using LC-MS analysis with ZIC-pHILIC chromatography. Data are presented as a mean of n = 3 ± SEM. Unpaired t test. ( B ) mRNA expression and protein concentration analysis of the acetyl-CoA-carboxylase (ACC). Basal ACC1 and ACC2 mRNA expression is determined by qRT-PCR and is related to the housekeeping gene RPL37A. Data are presented as a mean of n = 3 ± SEM. Unpaired t test with Welch’s correction. The ACC phospho/ACC total ratio protein concentration was determined by Western blot analysis using an anti-ACC total and anti-phospho-ACC antibody and is related to the housekeeper protein Hsp90. A representative blot is displayed. Data are presented as mean of n = 5 ± SEM. *p ≤ 0 . 05 , **p ≤ 0 . 01,***p ≤ 0.001 .

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Chromatography, Expressing, Protein Concentration, Quantitative RT-PCR, Western Blot

2) Product Images from "UGCG influences glutamine metabolism of breast cancer cells"

Article Title: UGCG influences glutamine metabolism of breast cancer cells

Journal: Scientific Reports

doi: 10.1038/s41598-019-52169-7

Analysis of metabolic flux of glutamine by 13 C 5 -L-glutamine supplementation and subsequent LC-MS analysis. ( A ) Relative abundance of GSH, GSSG and TCA cycle metabolites was determined by using LC-MS analysis with ZIC-pHILIC chromatography. Data are presented as a mean of n = 3 ± SEM. Unpaired t test. ( B ) mRNA expression and protein concentration analysis of the acetyl-CoA-carboxylase (ACC). Basal ACC1 and ACC2 mRNA expression is determined by qRT-PCR and is related to the housekeeping gene RPL37A. Data are presented as a mean of n = 3 ± SEM. Unpaired t test with Welch’s correction. The ACC phospho/ACC total ratio protein concentration was determined by Western blot analysis using an anti-ACC total and anti-phospho-ACC antibody and is related to the housekeeper protein Hsp90. A representative blot is displayed. Data are presented as mean of n = 5 ± SEM. *p ≤ 0 . 05 , **p ≤ 0 . 01,***p ≤ 0.001 .
Figure Legend Snippet: Analysis of metabolic flux of glutamine by 13 C 5 -L-glutamine supplementation and subsequent LC-MS analysis. ( A ) Relative abundance of GSH, GSSG and TCA cycle metabolites was determined by using LC-MS analysis with ZIC-pHILIC chromatography. Data are presented as a mean of n = 3 ± SEM. Unpaired t test. ( B ) mRNA expression and protein concentration analysis of the acetyl-CoA-carboxylase (ACC). Basal ACC1 and ACC2 mRNA expression is determined by qRT-PCR and is related to the housekeeping gene RPL37A. Data are presented as a mean of n = 3 ± SEM. Unpaired t test with Welch’s correction. The ACC phospho/ACC total ratio protein concentration was determined by Western blot analysis using an anti-ACC total and anti-phospho-ACC antibody and is related to the housekeeper protein Hsp90. A representative blot is displayed. Data are presented as mean of n = 5 ± SEM. *p ≤ 0 . 05 , **p ≤ 0 . 01,***p ≤ 0.001 .

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Chromatography, Expressing, Protein Concentration, Quantitative RT-PCR, Western Blot

Related Articles

High Performance Liquid Chromatography:

Article Title: Discovery of novel geranylgeranyl reductases and characterization of their substrate promiscuity
Article Snippet: .. The separation of FPP, GGPP, and their reduced forms was conducted on a ZIC-pHILIC column (150 mm length, 2.1 mm internal diameter, and 5 µm particle size, Merck) using an Agilent Technologies 1200 Series Rapid Resolution high-performance liquid chromatography (HPLC) system. .. Solvents for HPLC were purchased from HoneyWell and were of HPLC grade or higher.

Article Title: Benzoxaborole treatment perturbs S-adenosyl-L-methionine metabolism in Trypanosoma brucei
Article Snippet: All mass spectrometry of metabolite samples was carried out by Glasgow Polyomics. .. Metabolomics samples were separated by hydrophilic interaction liquid chromatography (HPLC) using a ZIC-pHILIC (polymer-based HPLC) column (Merck). ..

Article Title: Protective Role of Mytilus edulis Hydrolysate in Lipopolysaccharide-Galactosamine Acute Liver Injury
Article Snippet: The sustainability of the lab-scale pre-GMP production was confirmed by the IGMM (Institute of Genetics and Molecular Medicine) Mass Spectrometry laboratory (University of Edinburgh). .. N2-01 (in its undiluted form) was diluted 40:1 in 5:3:2 methanol:acetonitrile:water and 10 ul were injected onto a ZIC-pHILIC 4.6 mm x 150 mm Sequant column (Merck, United Kingdom) on an Ultimate 3000 series HPLC (Thermo Fisher Scientific, United States) with A gradient from 90 to 5% B in 20 min, where A was 20 mm ammonium carbonate and B was acetonitrile. .. Data were acquired on Q Exactive (Thermo Fisher Scientific, United States) with HESI in positive (75.5–1132.5) and negative (77.5–1132.5) mode at 70k resolution, with ddMS2 at 17.5k.

Liquid Chromatography with Mass Spectroscopy:

Article Title: UGCG influences glutamine metabolism of breast cancer cells
Article Snippet: The pellet was used for protein concentration determination and supernatant was stored at −80 °C prior to analysis. .. For the LC-MS method a 150 mm × 2.1 mm, 5 μm ZIC pHILIC column was used (Merck, VIC, Australia). ..

Hydrophilic Interaction Liquid Chromatography:

Article Title: Benzoxaborole treatment perturbs S-adenosyl-L-methionine metabolism in Trypanosoma brucei
Article Snippet: All mass spectrometry of metabolite samples was carried out by Glasgow Polyomics. .. Metabolomics samples were separated by hydrophilic interaction liquid chromatography (HPLC) using a ZIC-pHILIC (polymer-based HPLC) column (Merck). ..

Injection:

Article Title: Protective Role of Mytilus edulis Hydrolysate in Lipopolysaccharide-Galactosamine Acute Liver Injury
Article Snippet: The sustainability of the lab-scale pre-GMP production was confirmed by the IGMM (Institute of Genetics and Molecular Medicine) Mass Spectrometry laboratory (University of Edinburgh). .. N2-01 (in its undiluted form) was diluted 40:1 in 5:3:2 methanol:acetonitrile:water and 10 ul were injected onto a ZIC-pHILIC 4.6 mm x 150 mm Sequant column (Merck, United Kingdom) on an Ultimate 3000 series HPLC (Thermo Fisher Scientific, United States) with A gradient from 90 to 5% B in 20 min, where A was 20 mm ammonium carbonate and B was acetonitrile. .. Data were acquired on Q Exactive (Thermo Fisher Scientific, United States) with HESI in positive (75.5–1132.5) and negative (77.5–1132.5) mode at 70k resolution, with ddMS2 at 17.5k.

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    Merck & Co sequant zic philic column
    Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a <t>SeQuant®</t> <t>ZIC®-pHILIC</t> column, before (black) and after (red) enzyme digestion ( b )
    Sequant Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic philic column/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sequant zic philic column - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Merck & Co zic philic
    Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a <t>SeQuant®</t> <t>ZIC®-pHILIC</t> column, before (black) and after (red) enzyme digestion ( b )
    Zic Philic, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zic philic/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zic philic - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

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    Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a SeQuant® ZIC®-pHILIC column, before (black) and after (red) enzyme digestion ( b )

    Journal: BMC Microbiology

    Article Title: Utilization of different MurNAc sources by the oral pathogen Tannerella forsythia and role of the inner membrane transporter AmpG

    doi: 10.1186/s12866-020-02006-z

    Figure Lengend Snippet: Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a SeQuant® ZIC®-pHILIC column, before (black) and after (red) enzyme digestion ( b )

    Article Snippet: Five-microliters of each sample were analysed immediately by LS-MS run in negative ion mode as described previously [ ] in order to ensure the efficient separation of GlcNAc-MurNAc and MurNAc on a SeQuant® ZIC®-pHILIC column (Merck, PEEK 150 × 2.1 mm, 5 μm).

    Techniques: Incubation