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Merck & Co zic philic column
Analysis of metabolic flux of glutamine by 13 C 5 -L-glutamine supplementation and subsequent LC-MS analysis. ( A ) Relative abundance of GSH, GSSG and TCA cycle metabolites was determined by using LC-MS analysis with <t>ZIC-pHILIC</t> chromatography. Data are presented as a mean of n = 3 ± SEM. Unpaired t test. ( B ) mRNA expression and protein concentration analysis of the acetyl-CoA-carboxylase (ACC). Basal ACC1 and ACC2 mRNA expression is determined by qRT-PCR and is related to the housekeeping gene RPL37A. Data are presented as a mean of n = 3 ± SEM. Unpaired t test with Welch’s correction. The ACC phospho/ACC total ratio protein concentration was determined by Western blot analysis using an anti-ACC total and anti-phospho-ACC antibody and is related to the housekeeper protein Hsp90. A representative blot is displayed. Data are presented as mean of n = 5 ± SEM. *p ≤ 0 . 05 , **p ≤ 0 . 01,***p ≤ 0.001 .
Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zic philic column/product/Merck & Co
Average 92 stars, based on 59 article reviews
Price from $9.99 to $1999.99
zic philic column - by Bioz Stars, 2020-07
92/100 stars

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1) Product Images from "UGCG influences glutamine metabolism of breast cancer cells"

Article Title: UGCG influences glutamine metabolism of breast cancer cells

Journal: Scientific Reports

doi: 10.1038/s41598-019-52169-7

Analysis of metabolic flux of glutamine by 13 C 5 -L-glutamine supplementation and subsequent LC-MS analysis. ( A ) Relative abundance of GSH, GSSG and TCA cycle metabolites was determined by using LC-MS analysis with ZIC-pHILIC chromatography. Data are presented as a mean of n = 3 ± SEM. Unpaired t test. ( B ) mRNA expression and protein concentration analysis of the acetyl-CoA-carboxylase (ACC). Basal ACC1 and ACC2 mRNA expression is determined by qRT-PCR and is related to the housekeeping gene RPL37A. Data are presented as a mean of n = 3 ± SEM. Unpaired t test with Welch’s correction. The ACC phospho/ACC total ratio protein concentration was determined by Western blot analysis using an anti-ACC total and anti-phospho-ACC antibody and is related to the housekeeper protein Hsp90. A representative blot is displayed. Data are presented as mean of n = 5 ± SEM. *p ≤ 0 . 05 , **p ≤ 0 . 01,***p ≤ 0.001 .
Figure Legend Snippet: Analysis of metabolic flux of glutamine by 13 C 5 -L-glutamine supplementation and subsequent LC-MS analysis. ( A ) Relative abundance of GSH, GSSG and TCA cycle metabolites was determined by using LC-MS analysis with ZIC-pHILIC chromatography. Data are presented as a mean of n = 3 ± SEM. Unpaired t test. ( B ) mRNA expression and protein concentration analysis of the acetyl-CoA-carboxylase (ACC). Basal ACC1 and ACC2 mRNA expression is determined by qRT-PCR and is related to the housekeeping gene RPL37A. Data are presented as a mean of n = 3 ± SEM. Unpaired t test with Welch’s correction. The ACC phospho/ACC total ratio protein concentration was determined by Western blot analysis using an anti-ACC total and anti-phospho-ACC antibody and is related to the housekeeper protein Hsp90. A representative blot is displayed. Data are presented as mean of n = 5 ± SEM. *p ≤ 0 . 05 , **p ≤ 0 . 01,***p ≤ 0.001 .

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Chromatography, Expressing, Protein Concentration, Quantitative RT-PCR, Western Blot

Related Articles

Hydrophilic Interaction Liquid Chromatography:

Article Title: Reduced levels of dopamine and altered metabolism in brains of HPRT knock-out rats: a new rodent model of Lesch-Nyhan Disease
Article Snippet: .. LC-MS Samples (10 μl) were analysed by hydrophilic interaction liquid chromatography (HILIC)-mass spectrometry (LC-MS) (UltiMate 3000 RSLC (Thermo Fisher, San Jose, California, USA) using a 150 × 4.6 mm ZIC-pHILIC column (Merck SeQuant, Umea, Sweden) running at 300 ul/min and an Exactive (Thermo Scientific) for MS detection. .. Buffers consisted of A: 20 mM ammonium carbonate (Sigma Aldrich) in H2 O (Fisher Scientific) and B: acetonitrile (Fisher Scientific).

Injection:

Article Title: Metabolomics-Based Screening of the Malaria Box Reveals both Novel and Established Mechanisms of Action
Article Snippet: .. Samples (10 μl) were injected onto a Dionex RSLC U3000 LC system (Thermo) fitted with a ZIC-pHILIC column (5 μm, 4.6 by 150 mm; Merck) and 20 mM ammonium carbonate (A) and acetonitrile (B) as the mobile phases ( ). ..

Liquid Chromatography:

Article Title: Genetic regulation of mitotic competence in G0 quiescent cells
Article Snippet: .. Samples were spiked with two internal standards, Pipes and Hepes, extracted, and separated by liquid chromatography on a ZIC-pHILIC column (Merck), and then measured using an LTQ Orbitrap mass spectrometer (Thermo Fisher Scientific). .. MZmine 2 software was used for raw mass spectrum analysis ( ).

Mass Spectrometry:

Article Title: Genetic regulation of mitotic competence in G0 quiescent cells
Article Snippet: .. Samples were spiked with two internal standards, Pipes and Hepes, extracted, and separated by liquid chromatography on a ZIC-pHILIC column (Merck), and then measured using an LTQ Orbitrap mass spectrometer (Thermo Fisher Scientific). .. MZmine 2 software was used for raw mass spectrum analysis ( ).

Article Title: Reduced levels of dopamine and altered metabolism in brains of HPRT knock-out rats: a new rodent model of Lesch-Nyhan Disease
Article Snippet: .. LC-MS Samples (10 μl) were analysed by hydrophilic interaction liquid chromatography (HILIC)-mass spectrometry (LC-MS) (UltiMate 3000 RSLC (Thermo Fisher, San Jose, California, USA) using a 150 × 4.6 mm ZIC-pHILIC column (Merck SeQuant, Umea, Sweden) running at 300 ul/min and an Exactive (Thermo Scientific) for MS detection. .. Buffers consisted of A: 20 mM ammonium carbonate (Sigma Aldrich) in H2 O (Fisher Scientific) and B: acetonitrile (Fisher Scientific).

Liquid Chromatography with Mass Spectroscopy:

Article Title: Reduced levels of dopamine and altered metabolism in brains of HPRT knock-out rats: a new rodent model of Lesch-Nyhan Disease
Article Snippet: .. LC-MS Samples (10 μl) were analysed by hydrophilic interaction liquid chromatography (HILIC)-mass spectrometry (LC-MS) (UltiMate 3000 RSLC (Thermo Fisher, San Jose, California, USA) using a 150 × 4.6 mm ZIC-pHILIC column (Merck SeQuant, Umea, Sweden) running at 300 ul/min and an Exactive (Thermo Scientific) for MS detection. .. Buffers consisted of A: 20 mM ammonium carbonate (Sigma Aldrich) in H2 O (Fisher Scientific) and B: acetonitrile (Fisher Scientific).

Article Title: UGCG influences glutamine metabolism of breast cancer cells
Article Snippet: .. For the LC-MS method a 150 mm × 2.1 mm, 5 μm ZIC pHILIC column was used (Merck, VIC, Australia). ..

Article Title: Linking soil biology and chemistry in biological soil crust using isolate exometabolomics
Article Snippet: .. Samples were analyzed using normal-phase LC/MS with a ZIC-pHILIC column (150 × 2.1 mm, 3.5 μm 200 Å, Merck Sequant, Darmstadt, Germany) using an Agilent 1290 series UHPLC (Agilent Technologies, Santa Clara, California, USA). .. Chromatographic separation was achieved using two mobile phases, 5 mM ammonium acetate in water (A) and 90% acetonitrile with 5 mM ammonium acetate (B) at a flow rate of 0.25 mL/min with the following gradient: 100% B for 1.5 min, decreased to 50% B by 25 min, held until 29.9 min then returned to initial conditions by 30 min with a total runtime of 40 min.

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    Merck & Co zic philic column
    Analysis of metabolic flux of glutamine by 13 C 5 -L-glutamine supplementation and subsequent LC-MS analysis. ( A ) Relative abundance of GSH, GSSG and TCA cycle metabolites was determined by using LC-MS analysis with <t>ZIC-pHILIC</t> chromatography. Data are presented as a mean of n = 3 ± SEM. Unpaired t test. ( B ) mRNA expression and protein concentration analysis of the acetyl-CoA-carboxylase (ACC). Basal ACC1 and ACC2 mRNA expression is determined by qRT-PCR and is related to the housekeeping gene RPL37A. Data are presented as a mean of n = 3 ± SEM. Unpaired t test with Welch’s correction. The ACC phospho/ACC total ratio protein concentration was determined by Western blot analysis using an anti-ACC total and anti-phospho-ACC antibody and is related to the housekeeper protein Hsp90. A representative blot is displayed. Data are presented as mean of n = 5 ± SEM. *p ≤ 0 . 05 , **p ≤ 0 . 01,***p ≤ 0.001 .
    Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zic philic column/product/Merck & Co
    Average 92 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    zic philic column - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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    Analysis of metabolic flux of glutamine by 13 C 5 -L-glutamine supplementation and subsequent LC-MS analysis. ( A ) Relative abundance of GSH, GSSG and TCA cycle metabolites was determined by using LC-MS analysis with ZIC-pHILIC chromatography. Data are presented as a mean of n = 3 ± SEM. Unpaired t test. ( B ) mRNA expression and protein concentration analysis of the acetyl-CoA-carboxylase (ACC). Basal ACC1 and ACC2 mRNA expression is determined by qRT-PCR and is related to the housekeeping gene RPL37A. Data are presented as a mean of n = 3 ± SEM. Unpaired t test with Welch’s correction. The ACC phospho/ACC total ratio protein concentration was determined by Western blot analysis using an anti-ACC total and anti-phospho-ACC antibody and is related to the housekeeper protein Hsp90. A representative blot is displayed. Data are presented as mean of n = 5 ± SEM. *p ≤ 0 . 05 , **p ≤ 0 . 01,***p ≤ 0.001 .

    Journal: Scientific Reports

    Article Title: UGCG influences glutamine metabolism of breast cancer cells

    doi: 10.1038/s41598-019-52169-7

    Figure Lengend Snippet: Analysis of metabolic flux of glutamine by 13 C 5 -L-glutamine supplementation and subsequent LC-MS analysis. ( A ) Relative abundance of GSH, GSSG and TCA cycle metabolites was determined by using LC-MS analysis with ZIC-pHILIC chromatography. Data are presented as a mean of n = 3 ± SEM. Unpaired t test. ( B ) mRNA expression and protein concentration analysis of the acetyl-CoA-carboxylase (ACC). Basal ACC1 and ACC2 mRNA expression is determined by qRT-PCR and is related to the housekeeping gene RPL37A. Data are presented as a mean of n = 3 ± SEM. Unpaired t test with Welch’s correction. The ACC phospho/ACC total ratio protein concentration was determined by Western blot analysis using an anti-ACC total and anti-phospho-ACC antibody and is related to the housekeeper protein Hsp90. A representative blot is displayed. Data are presented as mean of n = 5 ± SEM. *p ≤ 0 . 05 , **p ≤ 0 . 01,***p ≤ 0.001 .

    Article Snippet: For the LC-MS method a 150 mm × 2.1 mm, 5 μm ZIC pHILIC column was used (Merck, VIC, Australia).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Chromatography, Expressing, Protein Concentration, Quantitative RT-PCR, Western Blot