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Avantor zic philic column
Zic Philic Column, supplied by Avantor, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zic philic column/product/Avantor
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
zic philic column - by Bioz Stars, 2021-07
86/100 stars

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Liquid Chromatography with Mass Spectroscopy:

Article Title: Towards a More Reliable Identification of Isomeric Metabolites Using Pattern Guided Retention Validation
Article Snippet: The supernatant was transferred to LC–MS vials and stored at − 80 ∘ C until analysis. .. LC–MS measurements were performed as described elsewhere [ ], utilizing high-resolution liquid chromatography (Thermo Fisher Scientific, Waltham, MA, USA) with a ZIC-pHILIC column (VWR) at pH 9.4 coupled via electrospray ionization (ESI) to an Orbitrap Q Exactive mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). .. The same preparation was measured twice by LC–MS 11 months apart on the same machine with LC-column replaced in between.

Liquid Chromatography:

Article Title: Towards a More Reliable Identification of Isomeric Metabolites Using Pattern Guided Retention Validation
Article Snippet: The supernatant was transferred to LC–MS vials and stored at − 80 ∘ C until analysis. .. LC–MS measurements were performed as described elsewhere [ ], utilizing high-resolution liquid chromatography (Thermo Fisher Scientific, Waltham, MA, USA) with a ZIC-pHILIC column (VWR) at pH 9.4 coupled via electrospray ionization (ESI) to an Orbitrap Q Exactive mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). .. The same preparation was measured twice by LC–MS 11 months apart on the same machine with LC-column replaced in between.

Mass Spectrometry:

Article Title: Towards a More Reliable Identification of Isomeric Metabolites Using Pattern Guided Retention Validation
Article Snippet: The supernatant was transferred to LC–MS vials and stored at − 80 ∘ C until analysis. .. LC–MS measurements were performed as described elsewhere [ ], utilizing high-resolution liquid chromatography (Thermo Fisher Scientific, Waltham, MA, USA) with a ZIC-pHILIC column (VWR) at pH 9.4 coupled via electrospray ionization (ESI) to an Orbitrap Q Exactive mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). .. The same preparation was measured twice by LC–MS 11 months apart on the same machine with LC-column replaced in between.

High Performance Liquid Chromatography:

Article Title: Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress
Article Snippet: Supernatants were transferred to glass HPLC vials and stored at −80 °C until analysis LC-MS analysis. .. For HPLC-MS analysis a ZIC-pHILIC column (SeQuant, VWR) was used and Exactive and Q-Exactive mass spectrometers (Thermo Fisher Scientific) were operated with electrospray (ESI) ionisation and polarity switching mode at scan range (m/z ) 75–1000 at a resolution of 25,000 at 200 m/z. .. Data were acquired using Xcalibur software (Thermo Fisher Scientific) and peak areas of metabolites were determined using TraceFinder software (Thermo Fisher Scientific) by identifying metabolites using mass and known retention time following in-house analysis of commercial standards on our systems.

Article Title: Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress
Article Snippet: Supernatants were transferred to glass HPLC vials and stored at −80 °C until analysis LC-MS analysis. .. For HPLC-MS analysis a ZIC-pHILIC column (SeQuant, VWR) was used and Exactive and Q-Exactive mass spectrometers (Thermo Fisher Scientific) were operated with electrospray (ESI) ionisation and polarity switching mode at scan range ( m/z ) 75–1000 at a resolution of 25,000 at 200 m/z . .. Data were acquired using Xcalibur software (Thermo Fisher Scientific) and peak areas of metabolites were determined using TraceFinder software (Thermo Fisher Scientific) by identifying metabolites using mass and known retention time following in-house analysis of commercial standards on our systems.

Article Title: ATG7 regulates energy metabolism, differentiation and survival of Philadelphia-chromosome-positive cells
Article Snippet: LC-MS analysis A Thermo Scientific Exactive Orbitrap mass spectrometer was used together with a Thermo Scientific Accela HPLC system (Thermo Scientific Waltham, MA, USA). .. The HPLC setup consisted of a ZIC-pHILIC column (SeQuant 150×2.1 mm, 5 µm, 1.50460.0001, Merck KGaA, Darmstadt, Germany), with a ZIC-pHILIC guard column (SeQuant 20 x2.1 mm, 1.50438.0001, Merck KGaA, Darmstadt, Germany) and an initial mobile phase of 20% 20 mM ammonium carbonate, pH 9.4 (Sigma-Aldrich, 74415), and 80% acetonitrile (VWR, HiperSolv Chromanorm, 83639.320). .. Cell and media extracts (5 µl) were injected and metabolites were separated over a 15-min mobile phase gradient, decreasing the acetonitrile content to 20%, at a flow rate of 200 μL/min and a column temperature of 45°C.

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    Avantor zic philic column
    Zic Philic Column, supplied by Avantor, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zic philic column/product/Avantor
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zic philic column - by Bioz Stars, 2021-07
    86/100 stars
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