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Carl Zeiss z1 light sheet fluorescence microscopy lsfm
Overview of experimental pipeline for Octopus vulgaris embryos. RNA in situ hybridization chain reaction version 3.0 (RNA-ISH) and immunohistochemistry (IHC) are followed by fructose-glycerol clearing and imaging with <t>Light</t> <t>Sheet</t> <t>Fluorescence</t> <t>Microscopy</t> <t>(LSFM).</t> The final images (3D images and Z-stack planes) as well as videos are acquired, processed and analyzed with ZEN (black edition) and ARIVIS VISION4D v.3.1.4 software. For developmental stage XV embryo (its size is approximately 1,25 mm x 0,88 mm), RNA-ISH Clearing Imaging Image Analysis takes approximately 7 days whereas, RNA-ISH IHC Clearing Imaging Image Analysis takes around 9 days. (This figure is designed using a resource from freepik.com ).
Z1 Light Sheet Fluorescence Microscopy Lsfm, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/z1 light sheet fluorescence microscopy lsfm/product/Carl Zeiss
Average 86 stars, based on 2 article reviews
Price from $9.99 to $1999.99
z1 light sheet fluorescence microscopy lsfm - by Bioz Stars, 2022-09
86/100 stars

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1) Product Images from "Optimization of Whole Mount RNA Multiplexed in situ Hybridization Chain Reaction With Immunohistochemistry, Clearing and Imaging to Visualize Octopus Embryonic Neurogenesis"

Article Title: Optimization of Whole Mount RNA Multiplexed in situ Hybridization Chain Reaction With Immunohistochemistry, Clearing and Imaging to Visualize Octopus Embryonic Neurogenesis

Journal: Frontiers in Physiology

doi: 10.3389/fphys.2022.882413

Overview of experimental pipeline for Octopus vulgaris embryos. RNA in situ hybridization chain reaction version 3.0 (RNA-ISH) and immunohistochemistry (IHC) are followed by fructose-glycerol clearing and imaging with Light Sheet Fluorescence Microscopy (LSFM). The final images (3D images and Z-stack planes) as well as videos are acquired, processed and analyzed with ZEN (black edition) and ARIVIS VISION4D v.3.1.4 software. For developmental stage XV embryo (its size is approximately 1,25 mm x 0,88 mm), RNA-ISH Clearing Imaging Image Analysis takes approximately 7 days whereas, RNA-ISH IHC Clearing Imaging Image Analysis takes around 9 days. (This figure is designed using a resource from freepik.com ).
Figure Legend Snippet: Overview of experimental pipeline for Octopus vulgaris embryos. RNA in situ hybridization chain reaction version 3.0 (RNA-ISH) and immunohistochemistry (IHC) are followed by fructose-glycerol clearing and imaging with Light Sheet Fluorescence Microscopy (LSFM). The final images (3D images and Z-stack planes) as well as videos are acquired, processed and analyzed with ZEN (black edition) and ARIVIS VISION4D v.3.1.4 software. For developmental stage XV embryo (its size is approximately 1,25 mm x 0,88 mm), RNA-ISH Clearing Imaging Image Analysis takes approximately 7 days whereas, RNA-ISH IHC Clearing Imaging Image Analysis takes around 9 days. (This figure is designed using a resource from freepik.com ).

Techniques Used: RNA In Situ Hybridization, In Situ Hybridization, Immunohistochemistry, Imaging, Fluorescence, Microscopy, Software

Whole Mount HCR v3.0 followed by fructose-glycerol clearing on an Octopus vulgaris embryo (developmental stage XV) imaged with LSFM. Top panel illustrates the merged 3D view from the posterior side of the embryo, and bottom panel shows a single plane of a coronal section. (A) Overview image showing the expression of Ov-elav and Ov-apolpp on a Stage XV embryo in 3D. Note that only high-level expression is retained on the merged view. DAPI (in grey) is used for nuclear labelling. (B–D) 3 individual channels from (A) . (E) Overview image showing the expression of Ov-elav and Ov-apolpp on a coronal section of Stage XV embryo. (F–H) 3 individual channels from (E) . Abbreviations: ar, arm; D, dorsal; ey, eye; fu, funnel; gg, gastric ganglion; LL, lateral lip; ma, mantle; n, neuropil; OL, optic lobe; SEM, supraesophageal mass; sg, stellate ganglion;SUB, subesophageal mass; V, ventral; y, yolk.
Figure Legend Snippet: Whole Mount HCR v3.0 followed by fructose-glycerol clearing on an Octopus vulgaris embryo (developmental stage XV) imaged with LSFM. Top panel illustrates the merged 3D view from the posterior side of the embryo, and bottom panel shows a single plane of a coronal section. (A) Overview image showing the expression of Ov-elav and Ov-apolpp on a Stage XV embryo in 3D. Note that only high-level expression is retained on the merged view. DAPI (in grey) is used for nuclear labelling. (B–D) 3 individual channels from (A) . (E) Overview image showing the expression of Ov-elav and Ov-apolpp on a coronal section of Stage XV embryo. (F–H) 3 individual channels from (E) . Abbreviations: ar, arm; D, dorsal; ey, eye; fu, funnel; gg, gastric ganglion; LL, lateral lip; ma, mantle; n, neuropil; OL, optic lobe; SEM, supraesophageal mass; sg, stellate ganglion;SUB, subesophageal mass; V, ventral; y, yolk.

Techniques Used: Expressing

Whole Mount multiplexed HCR v3.0-IHC followed by fructose-glycerol clearing on an Octopus vulgaris embryo (developmental stage XV) imaged with LSFM to visualize neurogenesis. (A) Overview image showing the expression of Ov-ascl1 and Ov-neuroD and presence of mitotic cells (PH3+) on a Stage XV embryo in 3D view. DAPI (in grey) is used for nuclear labelling. (B) Image illustrating mitotic PH3+ cells with DAPI which is an indication of successful IHC after HCR. (C) Multiplexed HCR Image of Ov-ascl1 and Ov-neuroD with DAPI. (D–G) Separate channels from (A) . (H) Overlay of Ov-ascl1 and Ov-neuroD show mutually exclusive expression. Yellow line indicates the transition zone area. (I) Overview image showing the expression of Ov-ascl1 and Ov-neuroD and presence of mitotic cells (PH3+) on a coronal section of Stage XV embryo. (J–M) 4 individual channels from (I) . Abbreviations: ar, arm; D, dorsal; ey, eye; fu, funnel; LL, lateral lip; ma, mantle; OL, optic lobe; SUB, subesophageal mass; V: ventral; y, yolk.
Figure Legend Snippet: Whole Mount multiplexed HCR v3.0-IHC followed by fructose-glycerol clearing on an Octopus vulgaris embryo (developmental stage XV) imaged with LSFM to visualize neurogenesis. (A) Overview image showing the expression of Ov-ascl1 and Ov-neuroD and presence of mitotic cells (PH3+) on a Stage XV embryo in 3D view. DAPI (in grey) is used for nuclear labelling. (B) Image illustrating mitotic PH3+ cells with DAPI which is an indication of successful IHC after HCR. (C) Multiplexed HCR Image of Ov-ascl1 and Ov-neuroD with DAPI. (D–G) Separate channels from (A) . (H) Overlay of Ov-ascl1 and Ov-neuroD show mutually exclusive expression. Yellow line indicates the transition zone area. (I) Overview image showing the expression of Ov-ascl1 and Ov-neuroD and presence of mitotic cells (PH3+) on a coronal section of Stage XV embryo. (J–M) 4 individual channels from (I) . Abbreviations: ar, arm; D, dorsal; ey, eye; fu, funnel; LL, lateral lip; ma, mantle; OL, optic lobe; SUB, subesophageal mass; V: ventral; y, yolk.

Techniques Used: Immunohistochemistry, Expressing

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    Carl Zeiss z1 light sheet fluorescence microscopy lsfm
    Overview of experimental pipeline for Octopus vulgaris embryos. RNA in situ hybridization chain reaction version 3.0 (RNA-ISH) and immunohistochemistry (IHC) are followed by fructose-glycerol clearing and imaging with <t>Light</t> <t>Sheet</t> <t>Fluorescence</t> <t>Microscopy</t> <t>(LSFM).</t> The final images (3D images and Z-stack planes) as well as videos are acquired, processed and analyzed with ZEN (black edition) and ARIVIS VISION4D v.3.1.4 software. For developmental stage XV embryo (its size is approximately 1,25 mm x 0,88 mm), RNA-ISH Clearing Imaging Image Analysis takes approximately 7 days whereas, RNA-ISH IHC Clearing Imaging Image Analysis takes around 9 days. (This figure is designed using a resource from freepik.com ).
    Z1 Light Sheet Fluorescence Microscopy Lsfm, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/z1 light sheet fluorescence microscopy lsfm/product/Carl Zeiss
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    z1 light sheet fluorescence microscopy lsfm - by Bioz Stars, 2022-09
    90/100 stars
      Buy from Supplier

    99
    Carl Zeiss light sheet fluorescence microscope
    Overview of experimental pipeline for Octopus vulgaris embryos. RNA in situ hybridization chain reaction version 3.0 (RNA-ISH) and immunohistochemistry (IHC) are followed by fructose-glycerol clearing and imaging with <t>Light</t> <t>Sheet</t> <t>Fluorescence</t> <t>Microscopy</t> <t>(LSFM).</t> The final images (3D images and Z-stack planes) as well as videos are acquired, processed and analyzed with ZEN (black edition) and ARIVIS VISION4D v.3.1.4 software. For developmental stage XV embryo (its size is approximately 1,25 mm x 0,88 mm), RNA-ISH Clearing Imaging Image Analysis takes approximately 7 days whereas, RNA-ISH IHC Clearing Imaging Image Analysis takes around 9 days. (This figure is designed using a resource from freepik.com ).
    Light Sheet Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/light sheet fluorescence microscope/product/Carl Zeiss
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    light sheet fluorescence microscope - by Bioz Stars, 2022-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Overview of experimental pipeline for Octopus vulgaris embryos. RNA in situ hybridization chain reaction version 3.0 (RNA-ISH) and immunohistochemistry (IHC) are followed by fructose-glycerol clearing and imaging with Light Sheet Fluorescence Microscopy (LSFM). The final images (3D images and Z-stack planes) as well as videos are acquired, processed and analyzed with ZEN (black edition) and ARIVIS VISION4D v.3.1.4 software. For developmental stage XV embryo (its size is approximately 1,25 mm x 0,88 mm), RNA-ISH Clearing Imaging Image Analysis takes approximately 7 days whereas, RNA-ISH IHC Clearing Imaging Image Analysis takes around 9 days. (This figure is designed using a resource from freepik.com ).

    Journal: Frontiers in Physiology

    Article Title: Optimization of Whole Mount RNA Multiplexed in situ Hybridization Chain Reaction With Immunohistochemistry, Clearing and Imaging to Visualize Octopus Embryonic Neurogenesis

    doi: 10.3389/fphys.2022.882413

    Figure Lengend Snippet: Overview of experimental pipeline for Octopus vulgaris embryos. RNA in situ hybridization chain reaction version 3.0 (RNA-ISH) and immunohistochemistry (IHC) are followed by fructose-glycerol clearing and imaging with Light Sheet Fluorescence Microscopy (LSFM). The final images (3D images and Z-stack planes) as well as videos are acquired, processed and analyzed with ZEN (black edition) and ARIVIS VISION4D v.3.1.4 software. For developmental stage XV embryo (its size is approximately 1,25 mm x 0,88 mm), RNA-ISH Clearing Imaging Image Analysis takes approximately 7 days whereas, RNA-ISH IHC Clearing Imaging Image Analysis takes around 9 days. (This figure is designed using a resource from freepik.com ).

    Article Snippet: Imaging was done using Zeiss Z1 Light sheet fluorescence microscopy (LSFM) (Carl Zeiss AG, Germany).

    Techniques: RNA In Situ Hybridization, In Situ Hybridization, Immunohistochemistry, Imaging, Fluorescence, Microscopy, Software

    Whole Mount HCR v3.0 followed by fructose-glycerol clearing on an Octopus vulgaris embryo (developmental stage XV) imaged with LSFM. Top panel illustrates the merged 3D view from the posterior side of the embryo, and bottom panel shows a single plane of a coronal section. (A) Overview image showing the expression of Ov-elav and Ov-apolpp on a Stage XV embryo in 3D. Note that only high-level expression is retained on the merged view. DAPI (in grey) is used for nuclear labelling. (B–D) 3 individual channels from (A) . (E) Overview image showing the expression of Ov-elav and Ov-apolpp on a coronal section of Stage XV embryo. (F–H) 3 individual channels from (E) . Abbreviations: ar, arm; D, dorsal; ey, eye; fu, funnel; gg, gastric ganglion; LL, lateral lip; ma, mantle; n, neuropil; OL, optic lobe; SEM, supraesophageal mass; sg, stellate ganglion;SUB, subesophageal mass; V, ventral; y, yolk.

    Journal: Frontiers in Physiology

    Article Title: Optimization of Whole Mount RNA Multiplexed in situ Hybridization Chain Reaction With Immunohistochemistry, Clearing and Imaging to Visualize Octopus Embryonic Neurogenesis

    doi: 10.3389/fphys.2022.882413

    Figure Lengend Snippet: Whole Mount HCR v3.0 followed by fructose-glycerol clearing on an Octopus vulgaris embryo (developmental stage XV) imaged with LSFM. Top panel illustrates the merged 3D view from the posterior side of the embryo, and bottom panel shows a single plane of a coronal section. (A) Overview image showing the expression of Ov-elav and Ov-apolpp on a Stage XV embryo in 3D. Note that only high-level expression is retained on the merged view. DAPI (in grey) is used for nuclear labelling. (B–D) 3 individual channels from (A) . (E) Overview image showing the expression of Ov-elav and Ov-apolpp on a coronal section of Stage XV embryo. (F–H) 3 individual channels from (E) . Abbreviations: ar, arm; D, dorsal; ey, eye; fu, funnel; gg, gastric ganglion; LL, lateral lip; ma, mantle; n, neuropil; OL, optic lobe; SEM, supraesophageal mass; sg, stellate ganglion;SUB, subesophageal mass; V, ventral; y, yolk.

    Article Snippet: Imaging was done using Zeiss Z1 Light sheet fluorescence microscopy (LSFM) (Carl Zeiss AG, Germany).

    Techniques: Expressing

    Whole Mount multiplexed HCR v3.0-IHC followed by fructose-glycerol clearing on an Octopus vulgaris embryo (developmental stage XV) imaged with LSFM to visualize neurogenesis. (A) Overview image showing the expression of Ov-ascl1 and Ov-neuroD and presence of mitotic cells (PH3+) on a Stage XV embryo in 3D view. DAPI (in grey) is used for nuclear labelling. (B) Image illustrating mitotic PH3+ cells with DAPI which is an indication of successful IHC after HCR. (C) Multiplexed HCR Image of Ov-ascl1 and Ov-neuroD with DAPI. (D–G) Separate channels from (A) . (H) Overlay of Ov-ascl1 and Ov-neuroD show mutually exclusive expression. Yellow line indicates the transition zone area. (I) Overview image showing the expression of Ov-ascl1 and Ov-neuroD and presence of mitotic cells (PH3+) on a coronal section of Stage XV embryo. (J–M) 4 individual channels from (I) . Abbreviations: ar, arm; D, dorsal; ey, eye; fu, funnel; LL, lateral lip; ma, mantle; OL, optic lobe; SUB, subesophageal mass; V: ventral; y, yolk.

    Journal: Frontiers in Physiology

    Article Title: Optimization of Whole Mount RNA Multiplexed in situ Hybridization Chain Reaction With Immunohistochemistry, Clearing and Imaging to Visualize Octopus Embryonic Neurogenesis

    doi: 10.3389/fphys.2022.882413

    Figure Lengend Snippet: Whole Mount multiplexed HCR v3.0-IHC followed by fructose-glycerol clearing on an Octopus vulgaris embryo (developmental stage XV) imaged with LSFM to visualize neurogenesis. (A) Overview image showing the expression of Ov-ascl1 and Ov-neuroD and presence of mitotic cells (PH3+) on a Stage XV embryo in 3D view. DAPI (in grey) is used for nuclear labelling. (B) Image illustrating mitotic PH3+ cells with DAPI which is an indication of successful IHC after HCR. (C) Multiplexed HCR Image of Ov-ascl1 and Ov-neuroD with DAPI. (D–G) Separate channels from (A) . (H) Overlay of Ov-ascl1 and Ov-neuroD show mutually exclusive expression. Yellow line indicates the transition zone area. (I) Overview image showing the expression of Ov-ascl1 and Ov-neuroD and presence of mitotic cells (PH3+) on a coronal section of Stage XV embryo. (J–M) 4 individual channels from (I) . Abbreviations: ar, arm; D, dorsal; ey, eye; fu, funnel; LL, lateral lip; ma, mantle; OL, optic lobe; SUB, subesophageal mass; V: ventral; y, yolk.

    Article Snippet: Imaging was done using Zeiss Z1 Light sheet fluorescence microscopy (LSFM) (Carl Zeiss AG, Germany).

    Techniques: Immunohistochemistry, Expressing