anti ptdins 3 4 5 p3 igm hrp  (Echelon Biosciences)


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    Echelon Biosciences anti ptdins 3 4 5 p3 igm hrp
    Anti Ptdins 3 4 5 P3 Igm Hrp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ptdins 3 4 5 p3 igm hrp/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ptdins 3 4 5 p3 igm hrp - by Bioz Stars, 2022-08
    93/100 stars

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  • 86
    Echelon Biosciences anti pip3 antibody
    Effect of DT97 on p110-δ kinase activity ( A ) Effect of individual p110 isoform knockdown on <t>PIP3</t> production. RPMI8226 cells were transfected with shRNA to scrambled control or isoforms PIK3CA, PIK3CB, PIK3CG or PIK3CD . PIP3 production was then determined from the transfected cells after puromycin selection. Data points represent the average value from triplicate measurements. ( B ) Dose-dependent effect of DT97 on purified p110-δ. Assays were performed in triplicate, values represent the mean and error bars represent the SD. ( C ) Effect of DT97 treatment on RPMI8226 cells after transfection with control shRNA or shRNA specific to individual PIK3C isoforms. Cells were transfected, selected in puromycin and cell viabiltiy determined using the XTT assay. Values represent the average of triplicate measurements. ( D ) Effect of DT97 and idelalisib on MM and CLL cell viability. The effect of each agent at 1 uM was determined using the XTT assay after treatment for 72 h. Values represent the average of triplicate measurements. ( E ) Effect of DT97 and idelalisib on apoptosis in MM and CLL cells. The effect of each agent at 1 uM was determined by counting the number of apoptotic cells after 24 h. Values represent the average of triplicate measurements.
    Anti Pip3 Antibody, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pip3 antibody/product/Echelon Biosciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti pip3 antibody - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    86
    Echelon Biosciences anti pip3
    Effect of DT97 on p110-δ kinase activity ( A ) Effect of individual p110 isoform knockdown on <t>PIP3</t> production. RPMI8226 cells were transfected with shRNA to scrambled control or isoforms PIK3CA, PIK3CB, PIK3CG or PIK3CD . PIP3 production was then determined from the transfected cells after puromycin selection. Data points represent the average value from triplicate measurements. ( B ) Dose-dependent effect of DT97 on purified p110-δ. Assays were performed in triplicate, values represent the mean and error bars represent the SD. ( C ) Effect of DT97 treatment on RPMI8226 cells after transfection with control shRNA or shRNA specific to individual PIK3C isoforms. Cells were transfected, selected in puromycin and cell viabiltiy determined using the XTT assay. Values represent the average of triplicate measurements. ( D ) Effect of DT97 and idelalisib on MM and CLL cell viability. The effect of each agent at 1 uM was determined using the XTT assay after treatment for 72 h. Values represent the average of triplicate measurements. ( E ) Effect of DT97 and idelalisib on apoptosis in MM and CLL cells. The effect of each agent at 1 uM was determined by counting the number of apoptotic cells after 24 h. Values represent the average of triplicate measurements.
    Anti Pip3, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pip3/product/Echelon Biosciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti pip3 - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    93
    Echelon Biosciences ptdins 3 4 5 p3
    Effect of DT97 on p110-δ kinase activity ( A ) Effect of individual p110 isoform knockdown on <t>PIP3</t> production. RPMI8226 cells were transfected with shRNA to scrambled control or isoforms PIK3CA, PIK3CB, PIK3CG or PIK3CD . PIP3 production was then determined from the transfected cells after puromycin selection. Data points represent the average value from triplicate measurements. ( B ) Dose-dependent effect of DT97 on purified p110-δ. Assays were performed in triplicate, values represent the mean and error bars represent the SD. ( C ) Effect of DT97 treatment on RPMI8226 cells after transfection with control shRNA or shRNA specific to individual PIK3C isoforms. Cells were transfected, selected in puromycin and cell viabiltiy determined using the XTT assay. Values represent the average of triplicate measurements. ( D ) Effect of DT97 and idelalisib on MM and CLL cell viability. The effect of each agent at 1 uM was determined using the XTT assay after treatment for 72 h. Values represent the average of triplicate measurements. ( E ) Effect of DT97 and idelalisib on apoptosis in MM and CLL cells. The effect of each agent at 1 uM was determined by counting the number of apoptotic cells after 24 h. Values represent the average of triplicate measurements.
    Ptdins 3 4 5 P3, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptdins 3 4 5 p3/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ptdins 3 4 5 p3 - by Bioz Stars, 2022-08
    93/100 stars
      Buy from Supplier

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    Effect of DT97 on p110-δ kinase activity ( A ) Effect of individual p110 isoform knockdown on PIP3 production. RPMI8226 cells were transfected with shRNA to scrambled control or isoforms PIK3CA, PIK3CB, PIK3CG or PIK3CD . PIP3 production was then determined from the transfected cells after puromycin selection. Data points represent the average value from triplicate measurements. ( B ) Dose-dependent effect of DT97 on purified p110-δ. Assays were performed in triplicate, values represent the mean and error bars represent the SD. ( C ) Effect of DT97 treatment on RPMI8226 cells after transfection with control shRNA or shRNA specific to individual PIK3C isoforms. Cells were transfected, selected in puromycin and cell viabiltiy determined using the XTT assay. Values represent the average of triplicate measurements. ( D ) Effect of DT97 and idelalisib on MM and CLL cell viability. The effect of each agent at 1 uM was determined using the XTT assay after treatment for 72 h. Values represent the average of triplicate measurements. ( E ) Effect of DT97 and idelalisib on apoptosis in MM and CLL cells. The effect of each agent at 1 uM was determined by counting the number of apoptotic cells after 24 h. Values represent the average of triplicate measurements.

    Journal: Oncotarget

    Article Title: High throughput chemical library screening identifies a novel p110-δ inhibitor that potentiates the anti-myeloma effect of bortezomib

    doi: 10.18632/oncotarget.9568

    Figure Lengend Snippet: Effect of DT97 on p110-δ kinase activity ( A ) Effect of individual p110 isoform knockdown on PIP3 production. RPMI8226 cells were transfected with shRNA to scrambled control or isoforms PIK3CA, PIK3CB, PIK3CG or PIK3CD . PIP3 production was then determined from the transfected cells after puromycin selection. Data points represent the average value from triplicate measurements. ( B ) Dose-dependent effect of DT97 on purified p110-δ. Assays were performed in triplicate, values represent the mean and error bars represent the SD. ( C ) Effect of DT97 treatment on RPMI8226 cells after transfection with control shRNA or shRNA specific to individual PIK3C isoforms. Cells were transfected, selected in puromycin and cell viabiltiy determined using the XTT assay. Values represent the average of triplicate measurements. ( D ) Effect of DT97 and idelalisib on MM and CLL cell viability. The effect of each agent at 1 uM was determined using the XTT assay after treatment for 72 h. Values represent the average of triplicate measurements. ( E ) Effect of DT97 and idelalisib on apoptosis in MM and CLL cells. The effect of each agent at 1 uM was determined by counting the number of apoptotic cells after 24 h. Values represent the average of triplicate measurements.

    Article Snippet: Triplicate reactions were incubated with anti-PIP3 antibody for 1 h at room temperature.

    Techniques: Activity Assay, Transfection, shRNA, Selection, Purification, XTT Assay

    PI3K catalytic activity in MM cells ( A ) Comparison of healthy (normal), MGUS and MM CD138 + cells. PIP3 production was measured using CD138 + cells from healthy individuals, MGUS or MM patients. Cells were incubated with substrate and the amount of PIP3 generated quantitated by ELISA according to the manufacturer's instructions. ( B ) PIP3 production from CD138 + cells of MM patients that were either clinical responders or non-responders to bortezomib-based therapy. ( C ) PIP3 production from bortezomib sensitive and resistant MM cells. Each assay contained approximately 10,000 cells. All assays were performed in triplicate, values shown represent the mean and error bars represent the SD.

    Journal: Oncotarget

    Article Title: High throughput chemical library screening identifies a novel p110-δ inhibitor that potentiates the anti-myeloma effect of bortezomib

    doi: 10.18632/oncotarget.9568

    Figure Lengend Snippet: PI3K catalytic activity in MM cells ( A ) Comparison of healthy (normal), MGUS and MM CD138 + cells. PIP3 production was measured using CD138 + cells from healthy individuals, MGUS or MM patients. Cells were incubated with substrate and the amount of PIP3 generated quantitated by ELISA according to the manufacturer's instructions. ( B ) PIP3 production from CD138 + cells of MM patients that were either clinical responders or non-responders to bortezomib-based therapy. ( C ) PIP3 production from bortezomib sensitive and resistant MM cells. Each assay contained approximately 10,000 cells. All assays were performed in triplicate, values shown represent the mean and error bars represent the SD.

    Article Snippet: Triplicate reactions were incubated with anti-PIP3 antibody for 1 h at room temperature.

    Techniques: Activity Assay, Incubation, Generated, Enzyme-linked Immunosorbent Assay

    Effect of DT97 on p110-δ kinase activity ( A ) Effect of individual p110 isoform knockdown on PIP3 production. RPMI8226 cells were transfected with shRNA to scrambled control or isoforms PIK3CA, PIK3CB, PIK3CG or PIK3CD . PIP3 production was then determined from the transfected cells after puromycin selection. Data points represent the average value from triplicate measurements. ( B ) Dose-dependent effect of DT97 on purified p110-δ. Assays were performed in triplicate, values represent the mean and error bars represent the SD. ( C ) Effect of DT97 treatment on RPMI8226 cells after transfection with control shRNA or shRNA specific to individual PIK3C isoforms. Cells were transfected, selected in puromycin and cell viabiltiy determined using the XTT assay. Values represent the average of triplicate measurements. ( D ) Effect of DT97 and idelalisib on MM and CLL cell viability. The effect of each agent at 1 uM was determined using the XTT assay after treatment for 72 h. Values represent the average of triplicate measurements. ( E ) Effect of DT97 and idelalisib on apoptosis in MM and CLL cells. The effect of each agent at 1 uM was determined by counting the number of apoptotic cells after 24 h. Values represent the average of triplicate measurements.

    Journal: Oncotarget

    Article Title: High throughput chemical library screening identifies a novel p110-δ inhibitor that potentiates the anti-myeloma effect of bortezomib

    doi: 10.18632/oncotarget.9568

    Figure Lengend Snippet: Effect of DT97 on p110-δ kinase activity ( A ) Effect of individual p110 isoform knockdown on PIP3 production. RPMI8226 cells were transfected with shRNA to scrambled control or isoforms PIK3CA, PIK3CB, PIK3CG or PIK3CD . PIP3 production was then determined from the transfected cells after puromycin selection. Data points represent the average value from triplicate measurements. ( B ) Dose-dependent effect of DT97 on purified p110-δ. Assays were performed in triplicate, values represent the mean and error bars represent the SD. ( C ) Effect of DT97 treatment on RPMI8226 cells after transfection with control shRNA or shRNA specific to individual PIK3C isoforms. Cells were transfected, selected in puromycin and cell viabiltiy determined using the XTT assay. Values represent the average of triplicate measurements. ( D ) Effect of DT97 and idelalisib on MM and CLL cell viability. The effect of each agent at 1 uM was determined using the XTT assay after treatment for 72 h. Values represent the average of triplicate measurements. ( E ) Effect of DT97 and idelalisib on apoptosis in MM and CLL cells. The effect of each agent at 1 uM was determined by counting the number of apoptotic cells after 24 h. Values represent the average of triplicate measurements.

    Article Snippet: Reactions were then transferred to plates coated with PIP3 and incubated for 1 h. After 3 washes with TBS + 0.05% Tween20 (TBST), 100 μl horseradish peroxidase (HRP)-conjugated antibody to the anti-PIP3 was added and incubation continued for 1 h in the dark.

    Techniques: Activity Assay, Transfection, shRNA, Selection, Purification, XTT Assay

    PI3K catalytic activity in MM cells ( A ) Comparison of healthy (normal), MGUS and MM CD138 + cells. PIP3 production was measured using CD138 + cells from healthy individuals, MGUS or MM patients. Cells were incubated with substrate and the amount of PIP3 generated quantitated by ELISA according to the manufacturer's instructions. ( B ) PIP3 production from CD138 + cells of MM patients that were either clinical responders or non-responders to bortezomib-based therapy. ( C ) PIP3 production from bortezomib sensitive and resistant MM cells. Each assay contained approximately 10,000 cells. All assays were performed in triplicate, values shown represent the mean and error bars represent the SD.

    Journal: Oncotarget

    Article Title: High throughput chemical library screening identifies a novel p110-δ inhibitor that potentiates the anti-myeloma effect of bortezomib

    doi: 10.18632/oncotarget.9568

    Figure Lengend Snippet: PI3K catalytic activity in MM cells ( A ) Comparison of healthy (normal), MGUS and MM CD138 + cells. PIP3 production was measured using CD138 + cells from healthy individuals, MGUS or MM patients. Cells were incubated with substrate and the amount of PIP3 generated quantitated by ELISA according to the manufacturer's instructions. ( B ) PIP3 production from CD138 + cells of MM patients that were either clinical responders or non-responders to bortezomib-based therapy. ( C ) PIP3 production from bortezomib sensitive and resistant MM cells. Each assay contained approximately 10,000 cells. All assays were performed in triplicate, values shown represent the mean and error bars represent the SD.

    Article Snippet: Reactions were then transferred to plates coated with PIP3 and incubated for 1 h. After 3 washes with TBS + 0.05% Tween20 (TBST), 100 μl horseradish peroxidase (HRP)-conjugated antibody to the anti-PIP3 was added and incubation continued for 1 h in the dark.

    Techniques: Activity Assay, Incubation, Generated, Enzyme-linked Immunosorbent Assay