ypda broth  (TaKaRa)

 
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    Name:
    YPDA Broth
    Description:
    YPD and YPDA yeast media pouches provide media aliquoted in highly convenient pouches Simply empty the contents of each into your media flask bottle add 0 5 L of dH2O and sterilize No weighing of individual supplements combining or pH adjustment is required All media pouch mixes are available either with agar or without broth
    Catalog Number:
    630306
    Price:
    None
    Size:
    10 x 0 5L Pouches
    Category:
    YPDA and YPD rich media Yeast media Two hybrid and one hybrid systems Protein research
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    Structured Review

    TaKaRa ypda broth
    YPD and YPDA yeast media pouches provide media aliquoted in highly convenient pouches Simply empty the contents of each into your media flask bottle add 0 5 L of dH2O and sterilize No weighing of individual supplements combining or pH adjustment is required All media pouch mixes are available either with agar or without broth
    https://www.bioz.com/result/ypda broth/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ypda broth - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Human neuroglial cells internalize Borrelia burgdorferi by coiling phagocytosis mediated by Daam1
    Article Snippet: Yeast 2-hybrid (Y2H) library and bait clone construction Saccharomyces cerevisiae yeast strains, Y2HGold and Y187 (Clontech Laboratories, Inc., Mountain View, CA) were cultivated in YPDA broth (Clontech), streaked, and stored on YPDA plates. .. Transformant bait and prey clones of Y2HGold and Y187 were cultivated in specialized broth and agar media purchased as premixed specialized pouches (Clontech).

    Article Title: Human neuroglial cells internalize Borrelia burgdorferi by coiling phagocytosis mediated by Daam1
    Article Snippet: Saccharomyces cerevisiae yeast strains, Y2HGold and Y187 (Clontech Laboratories, Inc., Mountain View, CA) were cultivated in YPDA broth (Clontech), streaked, and stored on YPDA plates. .. Transformant bait and prey clones of Y2HGold and Y187 were cultivated in specialized broth and agar media purchased as premixed specialized pouches (Clontech).

    Transfection:

    Article Title: Interaction of phosphorylated Rab11-FIP2 with Eps15 regulates apical junction composition
    Article Snippet: Yeast two-hybrid assay Yeast were transfected and tested for β-galactosidase activity using the colony lift assay as previously described ( ). .. For the plate assay, three colonies from each plate (triplicates) were inoculated into separate 4 ml of YPDA broth (yeast extract/peptone/dextrose [Clontech, Mountain View, CA] supplemented with 0.003% adenine hemisulfate [Sigma-Aldrich]) and grown overnight at 30°C.

    Selection:

    Article Title: Human neuroglial cells internalize Borrelia burgdorferi by coiling phagocytosis mediated by Daam1
    Article Snippet: Yeast 2-hybrid (Y2H) library and bait clone construction Saccharomyces cerevisiae yeast strains, Y2HGold and Y187 (Clontech Laboratories, Inc., Mountain View, CA) were cultivated in YPDA broth (Clontech), streaked, and stored on YPDA plates. .. Aureobasidin A was included as appropriate for antibiotic selection (Clontech).

    Article Title: Human neuroglial cells internalize Borrelia burgdorferi by coiling phagocytosis mediated by Daam1
    Article Snippet: Saccharomyces cerevisiae yeast strains, Y2HGold and Y187 (Clontech Laboratories, Inc., Mountain View, CA) were cultivated in YPDA broth (Clontech), streaked, and stored on YPDA plates. .. Aureobasidin A was included as appropriate for antibiotic selection (Clontech).).

    Activation Assay:

    Article Title: Human neuroglial cells internalize Borrelia burgdorferi by coiling phagocytosis mediated by Daam1
    Article Snippet: Yeast 2-hybrid (Y2H) library and bait clone construction Saccharomyces cerevisiae yeast strains, Y2HGold and Y187 (Clontech Laboratories, Inc., Mountain View, CA) were cultivated in YPDA broth (Clontech), streaked, and stored on YPDA plates. .. Yeast colonies were grown as appropriate on synthetically defined (SD) medium: SD base (supplies all nutrients needed for growth); SD/–Leu/–Trp (double) dropout supplement (selects for transformants containing bait and prey plasmids); SD/–Ade/–His/–Leu/–Trp (quadruple) dropout supplement (selects for the bait and prey plasmids, and for the activation of the Gal-responsive HIS3 and ADE2 genes as part of the confirmation step of the two-hybrid assay).

    Article Title: Human neuroglial cells internalize Borrelia burgdorferi by coiling phagocytosis mediated by Daam1
    Article Snippet: Saccharomyces cerevisiae yeast strains, Y2HGold and Y187 (Clontech Laboratories, Inc., Mountain View, CA) were cultivated in YPDA broth (Clontech), streaked, and stored on YPDA plates. .. Yeast colonies were grown as appropriate on synthetically defined (SD) medium: SD base (supplies all nutrients needed for growth); SD/–Leu/–Trp (double) dropout supplement (selects for transformants containing bait and prey plasmids); SD/–Ade/–His/–Leu/–Trp (quadruple) dropout supplement (selects for the bait and prey plasmids, and for the activation of the Gal-responsive HIS3 and ADE2 genes as part of the confirmation step of the two-hybrid assay).

    Activity Assay:

    Article Title: Interaction of phosphorylated Rab11-FIP2 with Eps15 regulates apical junction composition
    Article Snippet: Yeast two-hybrid assay Yeast were transfected and tested for β-galactosidase activity using the colony lift assay as previously described ( ). .. For the plate assay, three colonies from each plate (triplicates) were inoculated into separate 4 ml of YPDA broth (yeast extract/peptone/dextrose [Clontech, Mountain View, CA] supplemented with 0.003% adenine hemisulfate [Sigma-Aldrich]) and grown overnight at 30°C.

    Y2H Assay:

    Article Title: Interaction of phosphorylated Rab11-FIP2 with Eps15 regulates apical junction composition
    Article Snippet: Paragraph title: Yeast two-hybrid assay ... For the plate assay, three colonies from each plate (triplicates) were inoculated into separate 4 ml of YPDA broth (yeast extract/peptone/dextrose [Clontech, Mountain View, CA] supplemented with 0.003% adenine hemisulfate [Sigma-Aldrich]) and grown overnight at 30°C.

    Two Hybrid Assay:

    Article Title: Human neuroglial cells internalize Borrelia burgdorferi by coiling phagocytosis mediated by Daam1
    Article Snippet: Yeast 2-hybrid (Y2H) library and bait clone construction Saccharomyces cerevisiae yeast strains, Y2HGold and Y187 (Clontech Laboratories, Inc., Mountain View, CA) were cultivated in YPDA broth (Clontech), streaked, and stored on YPDA plates. .. Yeast colonies were grown as appropriate on synthetically defined (SD) medium: SD base (supplies all nutrients needed for growth); SD/–Leu/–Trp (double) dropout supplement (selects for transformants containing bait and prey plasmids); SD/–Ade/–His/–Leu/–Trp (quadruple) dropout supplement (selects for the bait and prey plasmids, and for the activation of the Gal-responsive HIS3 and ADE2 genes as part of the confirmation step of the two-hybrid assay).

    Article Title: Human neuroglial cells internalize Borrelia burgdorferi by coiling phagocytosis mediated by Daam1
    Article Snippet: Saccharomyces cerevisiae yeast strains, Y2HGold and Y187 (Clontech Laboratories, Inc., Mountain View, CA) were cultivated in YPDA broth (Clontech), streaked, and stored on YPDA plates. .. Yeast colonies were grown as appropriate on synthetically defined (SD) medium: SD base (supplies all nutrients needed for growth); SD/–Leu/–Trp (double) dropout supplement (selects for transformants containing bait and prey plasmids); SD/–Ade/–His/–Leu/–Trp (quadruple) dropout supplement (selects for the bait and prey plasmids, and for the activation of the Gal-responsive HIS3 and ADE2 genes as part of the confirmation step of the two-hybrid assay).

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  • 99
    TaKaRa ypda broth
    Ypda Broth, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ypda broth/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ypda broth - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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