yeast torula rna  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Ribonucleic acid from torula yeast
    Description:

    Catalog Number:
    r6875
    Price:
    None
    Applications:
    Ribonucleic acid (RNA) from torula yeast may be used as a substrate for studying ribonuclease activities of enzymes such as ribonuclease-A, ribonuclease T1 (RNAase) and bougainvillea xbuttiana antiviral protein 1 (BBAP1).
    Buy from Supplier


    Structured Review

    Millipore yeast torula rna
    Expression of ZFERV-related <t>RNA</t> in thymus. (A) Larval fish at 5 dpf were fixed and subjected to whole mount in situ hybridization with a <t>DIG-labeled</t> sense or antisense probe for the env gene, followed by colorimetric detection. (B and C) Contiguous sections (20 μm thick) of 3-month-old fish (right two panels) were subjected to in situ hybridization with probes as described in panel A. The section planes and main organs are indicated. Abbreviations: B, brain; E, esophagus; G, gill; H, heart; I, intestine; L, liver; M, muscle; T, thymus. The bilateral thymi hybridizing to the antisense probe are highlighted with arrows. (D) Sections (2 μm thick) of the thymus from 3-month-old fish were hybridized to fluorescein-labeled sense (left panel) or antisense (right panel) env probe, followed by fluorescence detection with the alkaline phosphatase-Fast Red system.

    https://www.bioz.com/result/yeast torula rna/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    yeast torula rna - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "Genome Structure and Thymic Expression of an Endogenous Retrovirus in Zebrafish"

    Article Title: Genome Structure and Thymic Expression of an Endogenous Retrovirus in Zebrafish

    Journal: Journal of Virology

    doi: 10.1128/JVI.78.2.899-911.2004

    Expression of ZFERV-related RNA in thymus. (A) Larval fish at 5 dpf were fixed and subjected to whole mount in situ hybridization with a DIG-labeled sense or antisense probe for the env gene, followed by colorimetric detection. (B and C) Contiguous sections (20 μm thick) of 3-month-old fish (right two panels) were subjected to in situ hybridization with probes as described in panel A. The section planes and main organs are indicated. Abbreviations: B, brain; E, esophagus; G, gill; H, heart; I, intestine; L, liver; M, muscle; T, thymus. The bilateral thymi hybridizing to the antisense probe are highlighted with arrows. (D) Sections (2 μm thick) of the thymus from 3-month-old fish were hybridized to fluorescein-labeled sense (left panel) or antisense (right panel) env probe, followed by fluorescence detection with the alkaline phosphatase-Fast Red system.
    Figure Legend Snippet: Expression of ZFERV-related RNA in thymus. (A) Larval fish at 5 dpf were fixed and subjected to whole mount in situ hybridization with a DIG-labeled sense or antisense probe for the env gene, followed by colorimetric detection. (B and C) Contiguous sections (20 μm thick) of 3-month-old fish (right two panels) were subjected to in situ hybridization with probes as described in panel A. The section planes and main organs are indicated. Abbreviations: B, brain; E, esophagus; G, gill; H, heart; I, intestine; L, liver; M, muscle; T, thymus. The bilateral thymi hybridizing to the antisense probe are highlighted with arrows. (D) Sections (2 μm thick) of the thymus from 3-month-old fish were hybridized to fluorescein-labeled sense (left panel) or antisense (right panel) env probe, followed by fluorescence detection with the alkaline phosphatase-Fast Red system.

    Techniques Used: Expressing, Fluorescence In Situ Hybridization, In Situ Hybridization, Labeling, Fluorescence

    Related Articles

    Clone Assay:

    Article Title: Vascular development in the vertebrate pancreas
    Article Snippet: Pre-hybridization solution recipe is as follows: 50% Formamide (Fisher), 5XSSC (pH 4.5), 50μg/ml Ribonucleic acid from Torula yeast, Type VI (Sigma), 1% SDS, 50μg/ml Heparin (Sigma). .. Dig-RNA probes were generated using clones from Open Biosystems, unless indicated otherwise: APJ , CoupTFII , Connexin40 , Dll4 , Endoglin , Endomucin from Invitrogen , ICAM2 , Nrp1 , Nrp2 , Pecam-1 , Rasip1 as described before , Sox18 ( ).

    Article Title: BMP and BMP receptor expression during murine organogenesis
    Article Snippet: Source of clones used to generate probes were as follows: Open biosystems: Bmp2 , Bmp4 , Bmp7 , BmpR1a , BmpR1b , BmpRII from Doug Melton lab (Clone #227), Noggin from Jill McMahon with permission from Richard Harland. .. Prehybridization solution (50% Formamide (Fisher BP227-100), 5xSSC, pH 4.5, 50μg/ml Ribonucleic acid from torula yeast, TypeVI (Sigma R6625), 1% SDS, 50μg/ml Heparin (Sigma H4784)) was added to probes to a concentration of 10μg/ml, as a stock solution and kept at −80°C.

    Amplification:

    Article Title: Human Nup98 regulates the localization and activity of DExH/D-box helicase DHX9
    Article Snippet: The remaining sample was treated with an equal volume of 2 x Proteinase K solution (0.2 mg/ml proteinase K, 40 mM Tris–HCl pH 7.5, 5 mM EDTA, 33.4 ng/ml GlycoBlue (ThermoFisher Scientific AM9515), 0.2 mg/ml total yeast RNA (Sigma-Aldrich R6625)) for 30 min at 37°C to digest protein bound to the beads and release RNA. .. PCR amplification products were resolved in 2% agarose gels in TBE buffer (100 mM Tris, 90 mM boric acid, and 1 mM EDTA) and visualized with SYBR Safe DNA Gel Stain (ThermoFisher Scientific, S33102) in a Safe Imager 2.0 Blue Light Transilluminator (ThermoFisher Scientific, G6600).

    Synthesized:

    Article Title: Vascular development in the vertebrate pancreas
    Article Snippet: cDNA containing plasmid was linearized using a one-cutter restriction enzyme and an antisense Digoxigenin(Dig)UTP-labeled RNA probe was synthesized. .. Pre-hybridization solution recipe is as follows: 50% Formamide (Fisher), 5XSSC (pH 4.5), 50μg/ml Ribonucleic acid from Torula yeast, Type VI (Sigma), 1% SDS, 50μg/ml Heparin (Sigma).

    Article Title: mPEG-PAMAM-G4 Nucleic Acid Nanocomplexes: Enhanced Stability, RNase Protection, and Activity of Splice Switching Oligomer and Poly I:C RNA
    Article Snippet: Bulk ribonucleic acid from Torula yeast (8000 g/mol) and Lambda phage deoxyribonucleic acid (31.5 × 103 kDA, at 6 mg/mL) were purchased from Sigma Aldrich and used as supplied. .. The 623 SSOs were obtained from Integrated DNA Technologies with phosphothioate and 2’-O-methyl modifications consisting of the following sequence: 5’-GTTATTCTTTAGAATGGTGC-3’. mPEG-PAMAM-G4 (MW 8423 g/mol) was synthesized following published methods., Heparin Lithium salt was purchased from MP Biomedicals, LLC.

    Blocking Assay:

    Article Title: Genome Structure and Thymic Expression of an Endogenous Retrovirus in Zebrafish
    Article Snippet: For hybridization, samples were incubated with prehybridization buffer (4× SSC [600 mM NaCl, 60 mM sodium citrate; pH 7], 50% [vol/vol] formamide) at 37°C for 10 min, followed by incubation at 42°C for 16 h in hybridization buffer (50% [vol/vol] formamide, 4× SSC, 1× Denhardt solution [200 mg/liter each for Ficoll 400, polyvinylpyrrolidone, and bovine serum albumin], 100 μg of heparin/ml, 0.1% Tween 20, 1 mg of yeast Torula RNA [Sigma]/ml) containing fluorescein- or digoxigenin [DIG]-labeled RNA probe (0.2 μg/ml). .. After two washes with 0.2× SSC at 60°C, samples were incubated with blocking solution (0.1 M maleic acid [pH 7.6], 150 mM NaCl, 2% blocking reagent [Roche]) for 1 h. Alkaline phosphatase-conjugated anti-DIG or alkaline phosphatase-conjugated anti-fluorescein antibody (Roche) was added to the blocking solution in a ratio of 1:1,000, and incubation continued for two more hours.

    Suction Filtration:

    Article Title: Purification and Characterization of Iso-Ribonucleases from a Novel Thermophilic Fungus
    Article Snippet: Preparation of Sample for RNase Purification Isolated cultures were transferred to Fernbach flasks containing 500 mL of YpSs broth containing 0.2% yeast RNA (Sigma Cat# R6625; Milwaukee, WI, USA). .. Cell mass was removed from the culture by vacuum filtration through coarse filter paper (Fisher Scientific Filter Paper P8 Cat# 09-790-12D; Pittsburgh, PA, USA).

    Incubation:

    Article Title: Genome Structure and Thymic Expression of an Endogenous Retrovirus in Zebrafish
    Article Snippet: .. For hybridization, samples were incubated with prehybridization buffer (4× SSC [600 mM NaCl, 60 mM sodium citrate; pH 7], 50% [vol/vol] formamide) at 37°C for 10 min, followed by incubation at 42°C for 16 h in hybridization buffer (50% [vol/vol] formamide, 4× SSC, 1× Denhardt solution [200 mg/liter each for Ficoll 400, polyvinylpyrrolidone, and bovine serum albumin], 100 μg of heparin/ml, 0.1% Tween 20, 1 mg of yeast Torula RNA [Sigma]/ml) containing fluorescein- or digoxigenin [DIG]-labeled RNA probe (0.2 μg/ml). .. After two washes with 0.2× SSC at 60°C, samples were incubated with blocking solution (0.1 M maleic acid [pH 7.6], 150 mM NaCl, 2% blocking reagent [Roche]) for 1 h. Alkaline phosphatase-conjugated anti-DIG or alkaline phosphatase-conjugated anti-fluorescein antibody (Roche) was added to the blocking solution in a ratio of 1:1,000, and incubation continued for two more hours.

    Article Title: Vascular development in the vertebrate pancreas
    Article Snippet: DNA was eliminated by adding 2μl RQ1 DNase I (Promega), incubated at 37°C for 15 min. Micro Bio-spin columns (Bio-RAD) were used to purify the RNA probe. .. Pre-hybridization solution recipe is as follows: 50% Formamide (Fisher), 5XSSC (pH 4.5), 50μg/ml Ribonucleic acid from Torula yeast, Type VI (Sigma), 1% SDS, 50μg/ml Heparin (Sigma).

    Article Title: Targeting the TR4 nuclear receptor-mediated lncTASR/AXL signaling with tretinoin increases the sunitinib sensitivity to better suppress the RCC progression
    Article Snippet: To test RNA–RNA interaction, 500 µl of the supernatant was incubated with a final concentration of 1 µM biotinylated AXL RNA bait (5′-TGA CAC CGA AGG GTG CCC GTG AGT C-3′). .. PierceTM Streptavidin Agarose (Thermo Fisher ScientificTM , #20347) beads (for RNA–RNA interaction) were pre-blocked by 10 µM RNA from torula yeast Type VI (MilliporeSigma, #R6625, St. Louis, MO) in PBS.

    Article Title: BMP and BMP receptor expression during murine organogenesis
    Article Snippet: 2μl RQ1 DNase1 was added (Promega M6101), and then incubated again at 37°C for 15 min. .. Prehybridization solution (50% Formamide (Fisher BP227-100), 5xSSC, pH 4.5, 50μg/ml Ribonucleic acid from torula yeast, TypeVI (Sigma R6625), 1% SDS, 50μg/ml Heparin (Sigma H4784)) was added to probes to a concentration of 10μg/ml, as a stock solution and kept at −80°C.

    Article Title: Purification and Characterization of Iso-Ribonucleases from a Novel Thermophilic Fungus
    Article Snippet: Preparation of Sample for RNase Purification Isolated cultures were transferred to Fernbach flasks containing 500 mL of YpSs broth containing 0.2% yeast RNA (Sigma Cat# R6625; Milwaukee, WI, USA). .. Each flask was placed on a rotary shaker set to 115 rpm and incubated at 55 °C.

    Article Title: Human Nup98 regulates the localization and activity of DExH/D-box helicase DHX9
    Article Snippet: Cleared cell lysates were added to antibody conjugated beads and incubated with rotation at 4°C for 2 hr. .. The remaining sample was treated with an equal volume of 2 x Proteinase K solution (0.2 mg/ml proteinase K, 40 mM Tris–HCl pH 7.5, 5 mM EDTA, 33.4 ng/ml GlycoBlue (ThermoFisher Scientific AM9515), 0.2 mg/ml total yeast RNA (Sigma-Aldrich R6625)) for 30 min at 37°C to digest protein bound to the beads and release RNA.

    Article Title: Tissue damage signaling is a prerequisite for protective neutrophil recruitment to microbial infection in zebrafish
    Article Snippet: .. Following removal of PBST, larvae were incubated in 1 mL HYB+ hybridization solution (50% formamide (AmericanBio), 5× SSC (AmericanBio), 0.1% Tween-20, 5 mg/mL yeast torula RNA (Sigma), 50 µg/µL heparin (Sigma-Aldrich)) for 4 h at 70 °C. .. After HYB+ was removed, 500 µL 1 ng/µL il1b probe diluted in HYB+ was added, and larvae were incubated overnight at 70°C.

    Activity Assay:

    Article Title: Purification of an Inducible DNase from a Thermophilic Fungus
    Article Snippet: .. The method used to detect RNase activity was identical to the described acid soluble assay method except yeast RNA (yeast RNA; 1mg/mL (Sigma R6625 Cat# R6625; Milwaukee, WI, USA) was substituted for the salmon sperm DNA. .. The membrane based system worked well and was faster than traditional column chromatography.

    Hybridization:

    Article Title: Genome Structure and Thymic Expression of an Endogenous Retrovirus in Zebrafish
    Article Snippet: .. For hybridization, samples were incubated with prehybridization buffer (4× SSC [600 mM NaCl, 60 mM sodium citrate; pH 7], 50% [vol/vol] formamide) at 37°C for 10 min, followed by incubation at 42°C for 16 h in hybridization buffer (50% [vol/vol] formamide, 4× SSC, 1× Denhardt solution [200 mg/liter each for Ficoll 400, polyvinylpyrrolidone, and bovine serum albumin], 100 μg of heparin/ml, 0.1% Tween 20, 1 mg of yeast Torula RNA [Sigma]/ml) containing fluorescein- or digoxigenin [DIG]-labeled RNA probe (0.2 μg/ml). .. After two washes with 0.2× SSC at 60°C, samples were incubated with blocking solution (0.1 M maleic acid [pH 7.6], 150 mM NaCl, 2% blocking reagent [Roche]) for 1 h. Alkaline phosphatase-conjugated anti-DIG or alkaline phosphatase-conjugated anti-fluorescein antibody (Roche) was added to the blocking solution in a ratio of 1:1,000, and incubation continued for two more hours.

    Article Title: Vascular development in the vertebrate pancreas
    Article Snippet: 10X hybridization stock was prepared at 10μg/ml by adding the appropriate volume of pre-hybridization solution onto the probe. .. Pre-hybridization solution recipe is as follows: 50% Formamide (Fisher), 5XSSC (pH 4.5), 50μg/ml Ribonucleic acid from Torula yeast, Type VI (Sigma), 1% SDS, 50μg/ml Heparin (Sigma).

    Article Title: Tissue damage signaling is a prerequisite for protective neutrophil recruitment to microbial infection in zebrafish
    Article Snippet: .. Following removal of PBST, larvae were incubated in 1 mL HYB+ hybridization solution (50% formamide (AmericanBio), 5× SSC (AmericanBio), 0.1% Tween-20, 5 mg/mL yeast torula RNA (Sigma), 50 µg/µL heparin (Sigma-Aldrich)) for 4 h at 70 °C. .. After HYB+ was removed, 500 µL 1 ng/µL il1b probe diluted in HYB+ was added, and larvae were incubated overnight at 70°C.

    Countercurrent Chromatography:

    Article Title: Targeting the TR4 nuclear receptor-mediated lncTASR/AXL signaling with tretinoin increases the sunitinib sensitivity to better suppress the RCC progression
    Article Snippet: To test RNA–RNA interaction, 500 µl of the supernatant was incubated with a final concentration of 1 µM biotinylated AXL RNA bait (5′-TGA CAC CGA AGG GTG CCC GTG AGT C-3′). .. PierceTM Streptavidin Agarose (Thermo Fisher ScientificTM , #20347) beads (for RNA–RNA interaction) were pre-blocked by 10 µM RNA from torula yeast Type VI (MilliporeSigma, #R6625, St. Louis, MO) in PBS.

    Immunoprecipitation:

    Article Title: Targeting the TR4 nuclear receptor-mediated lncTASR/AXL signaling with tretinoin increases the sunitinib sensitivity to better suppress the RCC progression
    Article Snippet: Paragraph title: RNA Immunoprecipitation (RIP) ... PierceTM Streptavidin Agarose (Thermo Fisher ScientificTM , #20347) beads (for RNA–RNA interaction) were pre-blocked by 10 µM RNA from torula yeast Type VI (MilliporeSigma, #R6625, St. Louis, MO) in PBS.

    Article Title: Human Nup98 regulates the localization and activity of DExH/D-box helicase DHX9
    Article Snippet: Paragraph title: RNA immunoprecipitation ... The remaining sample was treated with an equal volume of 2 x Proteinase K solution (0.2 mg/ml proteinase K, 40 mM Tris–HCl pH 7.5, 5 mM EDTA, 33.4 ng/ml GlycoBlue (ThermoFisher Scientific AM9515), 0.2 mg/ml total yeast RNA (Sigma-Aldrich R6625)) for 30 min at 37°C to digest protein bound to the beads and release RNA.

    Protease Inhibitor:

    Article Title: Targeting the TR4 nuclear receptor-mediated lncTASR/AXL signaling with tretinoin increases the sunitinib sensitivity to better suppress the RCC progression
    Article Snippet: RNA Immunoprecipitation (RIP) Cells were lysed in RIPA buffer supplemented with RNase and protease inhibitor cocktail. .. PierceTM Streptavidin Agarose (Thermo Fisher ScientificTM , #20347) beads (for RNA–RNA interaction) were pre-blocked by 10 µM RNA from torula yeast Type VI (MilliporeSigma, #R6625, St. Louis, MO) in PBS.

    Article Title: Human Nup98 regulates the localization and activity of DExH/D-box helicase DHX9
    Article Snippet: Beads were washed at 4°C with 1 ml of PBS cell lysis buffer, twice with 1 ml of high-salt buffer (5.3 mM KH2 PO4 , 775 mM NaCl, 14.18 mM Na2 HPO4 , 0.1% SDS, 0.5% sodium deoxycholate, 0.5% Igepal CA-630, protease inhibitor cocktail, and two units/µL of RNaseOUT Recombinant Ribonuclease Inhibitor), and then three times with 1 ml of PBS cell lysis buffer. .. The remaining sample was treated with an equal volume of 2 x Proteinase K solution (0.2 mg/ml proteinase K, 40 mM Tris–HCl pH 7.5, 5 mM EDTA, 33.4 ng/ml GlycoBlue (ThermoFisher Scientific AM9515), 0.2 mg/ml total yeast RNA (Sigma-Aldrich R6625)) for 30 min at 37°C to digest protein bound to the beads and release RNA.

    Generated:

    Article Title: Vascular development in the vertebrate pancreas
    Article Snippet: Pre-hybridization solution recipe is as follows: 50% Formamide (Fisher), 5XSSC (pH 4.5), 50μg/ml Ribonucleic acid from Torula yeast, Type VI (Sigma), 1% SDS, 50μg/ml Heparin (Sigma). .. Dig-RNA probes were generated using clones from Open Biosystems, unless indicated otherwise: APJ , CoupTFII , Connexin40 , Dll4 , Endoglin , Endomucin from Invitrogen , ICAM2 , Nrp1 , Nrp2 , Pecam-1 , Rasip1 as described before , Sox18 ( ).

    other:

    Article Title: Expression of the methionine sulfoxide reductase lost during evolution extends Drosophila lifespan in a methionine-dependent manner
    Article Snippet: Briefly, 101.07 g Diet TD.10417 (Harlan Teklad), 100 mg lecitin from soybean (Sigma), 500 mg ribonucleic acid from Torula yeast (Sigma), 100 g of dextrose, 20 g agar, 2.85 ml propionic acid, 0.255 ml of phosphoric acid (Sigma) and indicated amounts of Met (Sigma) per liter of water were used.

    Article Title: In situ hybridization assay-based small molecule screening in zebrafish
    Article Snippet: 500 ml 100% formamide (Invitrogen, cat. no. 15515-026) 250 ml 20X SSC 1ml Tween-20 50 μg/ml heparin (Sigma, H3393) 500 μg/ml total yeast RNA (Sigma, R6625) Add DEPC H2 O to final volume of 1L Store up to 1 year at -20°C

    Polymerase Chain Reaction:

    Article Title: Human Nup98 regulates the localization and activity of DExH/D-box helicase DHX9
    Article Snippet: The remaining sample was treated with an equal volume of 2 x Proteinase K solution (0.2 mg/ml proteinase K, 40 mM Tris–HCl pH 7.5, 5 mM EDTA, 33.4 ng/ml GlycoBlue (ThermoFisher Scientific AM9515), 0.2 mg/ml total yeast RNA (Sigma-Aldrich R6625)) for 30 min at 37°C to digest protein bound to the beads and release RNA. .. Reverse transcription reactions were performed on 1 µg of purified RNA using random primers (ThermoFisher Scientific 48190011) and Superscript II reverse transcriptase kit reagents (ThermoFisher Scientific 18064014) in a total volume of 20 µl. cDNA from the reverse transcription reaction (5 µl) was used as template in a 25 µl PCR reaction using primers described in the and Phusion High-Fidelity DNA Polymerase (New England BioLabs, M0530S).

    Recombinant:

    Article Title: Human Nup98 regulates the localization and activity of DExH/D-box helicase DHX9
    Article Snippet: Sample cross-linking was reversed by incubating beads with 140 µl of reverse buffer (10 mM Tris–HCl, pH 6.8, 5 mM EDTA, 10 mM DTT and two units/µL of RNaseOUT Recombinant Ribonuclease Inhibitor) for 45 min at 70°C. .. The remaining sample was treated with an equal volume of 2 x Proteinase K solution (0.2 mg/ml proteinase K, 40 mM Tris–HCl pH 7.5, 5 mM EDTA, 33.4 ng/ml GlycoBlue (ThermoFisher Scientific AM9515), 0.2 mg/ml total yeast RNA (Sigma-Aldrich R6625)) for 30 min at 37°C to digest protein bound to the beads and release RNA.

    Isolation:

    Article Title: Purification and Characterization of Iso-Ribonucleases from a Novel Thermophilic Fungus
    Article Snippet: .. Preparation of Sample for RNase Purification Isolated cultures were transferred to Fernbach flasks containing 500 mL of YpSs broth containing 0.2% yeast RNA (Sigma Cat# R6625; Milwaukee, WI, USA). .. Each flask was placed on a rotary shaker set to 115 rpm and incubated at 55 °C.

    Size-exclusion Chromatography:

    Article Title: Purification of an Inducible DNase from a Thermophilic Fungus
    Article Snippet: The purification of the DNase was achieved using size exclusion chromatography and an affinity based membrane purification system. .. The method used to detect RNase activity was identical to the described acid soluble assay method except yeast RNA (yeast RNA; 1mg/mL (Sigma R6625 Cat# R6625; Milwaukee, WI, USA) was substituted for the salmon sperm DNA.

    Labeling:

    Article Title: Vascular development in the vertebrate pancreas
    Article Snippet: Probe synthesis was performed at 37°C for 2 hours: 1μg linearized plasmid, 2.0μl 10X transcription buffer (Roche), 2.0μl DIG-RNA labeling mix (Roche), 1.5μl Placental ribonuclease inhibitor (Promega), 1.0μl T3, T7 or SP6 RNA polymerase (Roche) depending on plasmid, RNase-free water to a final volume of 20μl. .. Pre-hybridization solution recipe is as follows: 50% Formamide (Fisher), 5XSSC (pH 4.5), 50μg/ml Ribonucleic acid from Torula yeast, Type VI (Sigma), 1% SDS, 50μg/ml Heparin (Sigma).

    Article Title: BMP and BMP receptor expression during murine organogenesis
    Article Snippet: The reagents were mixed in the following order at room temperature: linearized plasmid (1μg), DIG-RNA labeling mix (Roche 11277073910) 2.0μl, 10x transcription buffer (Roche 1465384) 2μl, Placental ribonuclease inhibitor (Promega N2111) 2μl, RNA polymerase (Roche) 1μl, and double distilled RNase free water to a final volume of 20μl. .. Prehybridization solution (50% Formamide (Fisher BP227-100), 5xSSC, pH 4.5, 50μg/ml Ribonucleic acid from torula yeast, TypeVI (Sigma R6625), 1% SDS, 50μg/ml Heparin (Sigma H4784)) was added to probes to a concentration of 10μg/ml, as a stock solution and kept at −80°C.

    Purification:

    Article Title: BMP and BMP receptor expression during murine organogenesis
    Article Snippet: The probes were then purified with Micro Bio-spin colums (Bio-RAD 732-6250). .. Prehybridization solution (50% Formamide (Fisher BP227-100), 5xSSC, pH 4.5, 50μg/ml Ribonucleic acid from torula yeast, TypeVI (Sigma R6625), 1% SDS, 50μg/ml Heparin (Sigma H4784)) was added to probes to a concentration of 10μg/ml, as a stock solution and kept at −80°C.

    Article Title: Purification and Characterization of Iso-Ribonucleases from a Novel Thermophilic Fungus
    Article Snippet: .. Preparation of Sample for RNase Purification Isolated cultures were transferred to Fernbach flasks containing 500 mL of YpSs broth containing 0.2% yeast RNA (Sigma Cat# R6625; Milwaukee, WI, USA). .. Each flask was placed on a rotary shaker set to 115 rpm and incubated at 55 °C.

    Article Title: Purification of an Inducible DNase from a Thermophilic Fungus
    Article Snippet: The final purified enzyme sample was tested for RNase activity to see if the purified enzyme was a non-specific nuclease; no RNase activity was detected. .. The method used to detect RNase activity was identical to the described acid soluble assay method except yeast RNA (yeast RNA; 1mg/mL (Sigma R6625 Cat# R6625; Milwaukee, WI, USA) was substituted for the salmon sperm DNA.

    Article Title: Human Nup98 regulates the localization and activity of DExH/D-box helicase DHX9
    Article Snippet: The remaining sample was treated with an equal volume of 2 x Proteinase K solution (0.2 mg/ml proteinase K, 40 mM Tris–HCl pH 7.5, 5 mM EDTA, 33.4 ng/ml GlycoBlue (ThermoFisher Scientific AM9515), 0.2 mg/ml total yeast RNA (Sigma-Aldrich R6625)) for 30 min at 37°C to digest protein bound to the beads and release RNA. .. RNA was subsequently purified using TRIzol LS Reagent (ThermoFisher Scientific 10296–010) and treated with DNase I (ThermoFisher Scientific 18068015) before quantification.

    Sequencing:

    Article Title: mPEG-PAMAM-G4 Nucleic Acid Nanocomplexes: Enhanced Stability, RNase Protection, and Activity of Splice Switching Oligomer and Poly I:C RNA
    Article Snippet: Bulk ribonucleic acid from Torula yeast (8000 g/mol) and Lambda phage deoxyribonucleic acid (31.5 × 103 kDA, at 6 mg/mL) were purchased from Sigma Aldrich and used as supplied. .. The 623 SSOs were obtained from Integrated DNA Technologies with phosphothioate and 2’-O-methyl modifications consisting of the following sequence: 5’-GTTATTCTTTAGAATGGTGC-3’. mPEG-PAMAM-G4 (MW 8423 g/mol) was synthesized following published methods., Heparin Lithium salt was purchased from MP Biomedicals, LLC.

    RNA Extraction:

    Article Title: Targeting the TR4 nuclear receptor-mediated lncTASR/AXL signaling with tretinoin increases the sunitinib sensitivity to better suppress the RCC progression
    Article Snippet: PierceTM Streptavidin Agarose (Thermo Fisher ScientificTM , #20347) beads (for RNA–RNA interaction) were pre-blocked by 10 µM RNA from torula yeast Type VI (MilliporeSigma, #R6625, St. Louis, MO) in PBS. .. The RNA/antibody or RNA/RNA complex was washed four times before RNA extraction, which is the same as described in qRT-PCR section.

    Quantitative RT-PCR:

    Article Title: Targeting the TR4 nuclear receptor-mediated lncTASR/AXL signaling with tretinoin increases the sunitinib sensitivity to better suppress the RCC progression
    Article Snippet: PierceTM Streptavidin Agarose (Thermo Fisher ScientificTM , #20347) beads (for RNA–RNA interaction) were pre-blocked by 10 µM RNA from torula yeast Type VI (MilliporeSigma, #R6625, St. Louis, MO) in PBS. .. The RNA/antibody or RNA/RNA complex was washed four times before RNA extraction, which is the same as described in qRT-PCR section.

    Staining:

    Article Title: Genome Structure and Thymic Expression of an Endogenous Retrovirus in Zebrafish
    Article Snippet: For hybridization, samples were incubated with prehybridization buffer (4× SSC [600 mM NaCl, 60 mM sodium citrate; pH 7], 50% [vol/vol] formamide) at 37°C for 10 min, followed by incubation at 42°C for 16 h in hybridization buffer (50% [vol/vol] formamide, 4× SSC, 1× Denhardt solution [200 mg/liter each for Ficoll 400, polyvinylpyrrolidone, and bovine serum albumin], 100 μg of heparin/ml, 0.1% Tween 20, 1 mg of yeast Torula RNA [Sigma]/ml) containing fluorescein- or digoxigenin [DIG]-labeled RNA probe (0.2 μg/ml). .. For colorimetric detection, samples containing anti-DIG antibodies were washed once with alkaline phosphatase buffer (0.1 M Tris-HCl [pH 9.5], 0.1 M NaCl, 50 mM MgCl2 ), followed by staining in buffer containing 1 mM levamisole, 0.9 mg of nitroblue tetrazolium salt and 0.35 mg of BCIP (5-bromo-4-chloro-3-indolylphosphate; Roche)/ml.

    Article Title: Human Nup98 regulates the localization and activity of DExH/D-box helicase DHX9
    Article Snippet: The remaining sample was treated with an equal volume of 2 x Proteinase K solution (0.2 mg/ml proteinase K, 40 mM Tris–HCl pH 7.5, 5 mM EDTA, 33.4 ng/ml GlycoBlue (ThermoFisher Scientific AM9515), 0.2 mg/ml total yeast RNA (Sigma-Aldrich R6625)) for 30 min at 37°C to digest protein bound to the beads and release RNA. .. PCR amplification products were resolved in 2% agarose gels in TBE buffer (100 mM Tris, 90 mM boric acid, and 1 mM EDTA) and visualized with SYBR Safe DNA Gel Stain (ThermoFisher Scientific, S33102) in a Safe Imager 2.0 Blue Light Transilluminator (ThermoFisher Scientific, G6600).

    In Situ Hybridization:

    Article Title: Genome Structure and Thymic Expression of an Endogenous Retrovirus in Zebrafish
    Article Snippet: Paragraph title: In situ hybridization. ... For hybridization, samples were incubated with prehybridization buffer (4× SSC [600 mM NaCl, 60 mM sodium citrate; pH 7], 50% [vol/vol] formamide) at 37°C for 10 min, followed by incubation at 42°C for 16 h in hybridization buffer (50% [vol/vol] formamide, 4× SSC, 1× Denhardt solution [200 mg/liter each for Ficoll 400, polyvinylpyrrolidone, and bovine serum albumin], 100 μg of heparin/ml, 0.1% Tween 20, 1 mg of yeast Torula RNA [Sigma]/ml) containing fluorescein- or digoxigenin [DIG]-labeled RNA probe (0.2 μg/ml).

    Article Title: BMP and BMP receptor expression during murine organogenesis
    Article Snippet: Paragraph title: 2.2 Whole mount in situ hybridization ... Prehybridization solution (50% Formamide (Fisher BP227-100), 5xSSC, pH 4.5, 50μg/ml Ribonucleic acid from torula yeast, TypeVI (Sigma R6625), 1% SDS, 50μg/ml Heparin (Sigma H4784)) was added to probes to a concentration of 10μg/ml, as a stock solution and kept at −80°C.

    Article Title: Tissue damage signaling is a prerequisite for protective neutrophil recruitment to microbial infection in zebrafish
    Article Snippet: Paragraph title: Whole mount in situ hybridization (WISH) ... Following removal of PBST, larvae were incubated in 1 mL HYB+ hybridization solution (50% formamide (AmericanBio), 5× SSC (AmericanBio), 0.1% Tween-20, 5 mg/mL yeast torula RNA (Sigma), 50 µg/µL heparin (Sigma-Aldrich)) for 4 h at 70 °C.

    Plasmid Preparation:

    Article Title: Vascular development in the vertebrate pancreas
    Article Snippet: Probe synthesis was performed at 37°C for 2 hours: 1μg linearized plasmid, 2.0μl 10X transcription buffer (Roche), 2.0μl DIG-RNA labeling mix (Roche), 1.5μl Placental ribonuclease inhibitor (Promega), 1.0μl T3, T7 or SP6 RNA polymerase (Roche) depending on plasmid, RNase-free water to a final volume of 20μl. .. Pre-hybridization solution recipe is as follows: 50% Formamide (Fisher), 5XSSC (pH 4.5), 50μg/ml Ribonucleic acid from Torula yeast, Type VI (Sigma), 1% SDS, 50μg/ml Heparin (Sigma).

    Article Title: BMP and BMP receptor expression during murine organogenesis
    Article Snippet: The reagents were mixed in the following order at room temperature: linearized plasmid (1μg), DIG-RNA labeling mix (Roche 11277073910) 2.0μl, 10x transcription buffer (Roche 1465384) 2μl, Placental ribonuclease inhibitor (Promega N2111) 2μl, RNA polymerase (Roche) 1μl, and double distilled RNase free water to a final volume of 20μl. .. Prehybridization solution (50% Formamide (Fisher BP227-100), 5xSSC, pH 4.5, 50μg/ml Ribonucleic acid from torula yeast, TypeVI (Sigma R6625), 1% SDS, 50μg/ml Heparin (Sigma H4784)) was added to probes to a concentration of 10μg/ml, as a stock solution and kept at −80°C.

    Negative Control:

    Article Title: Human Nup98 regulates the localization and activity of DExH/D-box helicase DHX9
    Article Snippet: Anti-GFP rabbit polyclonal antibodies were used as a negative control in immunoprecipitation reactions and are identified as control IgG in figures. .. The remaining sample was treated with an equal volume of 2 x Proteinase K solution (0.2 mg/ml proteinase K, 40 mM Tris–HCl pH 7.5, 5 mM EDTA, 33.4 ng/ml GlycoBlue (ThermoFisher Scientific AM9515), 0.2 mg/ml total yeast RNA (Sigma-Aldrich R6625)) for 30 min at 37°C to digest protein bound to the beads and release RNA.

    Column Chromatography:

    Article Title: Purification of an Inducible DNase from a Thermophilic Fungus
    Article Snippet: The method used to detect RNase activity was identical to the described acid soluble assay method except yeast RNA (yeast RNA; 1mg/mL (Sigma R6625 Cat# R6625; Milwaukee, WI, USA) was substituted for the salmon sperm DNA. .. The membrane based system worked well and was faster than traditional column chromatography.

    Produced:

    Article Title: Purification of an Inducible DNase from a Thermophilic Fungus
    Article Snippet: For example, Chaetomium thermophile was found to produce ascospores at 45 °C but at higher temperatures only produced sterile ascocarps or mycelium [ ]. .. The method used to detect RNase activity was identical to the described acid soluble assay method except yeast RNA (yeast RNA; 1mg/mL (Sigma R6625 Cat# R6625; Milwaukee, WI, USA) was substituted for the salmon sperm DNA.

    Concentration Assay:

    Article Title: mPEG-PAMAM-G4 Nucleic Acid Nanocomplexes: Enhanced Stability, RNase Protection, and Activity of Splice Switching Oligomer and Poly I:C RNA
    Article Snippet: Bulk ribonucleic acid from Torula yeast (8000 g/mol) and Lambda phage deoxyribonucleic acid (31.5 × 103 kDA, at 6 mg/mL) were purchased from Sigma Aldrich and used as supplied. .. RNase A was purchased from Sigma Aldrich and was utilized at a 0.6 mg/mL concentration.

    Article Title: Targeting the TR4 nuclear receptor-mediated lncTASR/AXL signaling with tretinoin increases the sunitinib sensitivity to better suppress the RCC progression
    Article Snippet: To test RNA–RNA interaction, 500 µl of the supernatant was incubated with a final concentration of 1 µM biotinylated AXL RNA bait (5′-TGA CAC CGA AGG GTG CCC GTG AGT C-3′). .. PierceTM Streptavidin Agarose (Thermo Fisher ScientificTM , #20347) beads (for RNA–RNA interaction) were pre-blocked by 10 µM RNA from torula yeast Type VI (MilliporeSigma, #R6625, St. Louis, MO) in PBS.

    Article Title: BMP and BMP receptor expression during murine organogenesis
    Article Snippet: .. Prehybridization solution (50% Formamide (Fisher BP227-100), 5xSSC, pH 4.5, 50μg/ml Ribonucleic acid from torula yeast, TypeVI (Sigma R6625), 1% SDS, 50μg/ml Heparin (Sigma H4784)) was added to probes to a concentration of 10μg/ml, as a stock solution and kept at −80°C. ..

    Lysis:

    Article Title: Human Nup98 regulates the localization and activity of DExH/D-box helicase DHX9
    Article Snippet: Beads were washed at 4°C with 1 ml of PBS cell lysis buffer, twice with 1 ml of high-salt buffer (5.3 mM KH2 PO4 , 775 mM NaCl, 14.18 mM Na2 HPO4 , 0.1% SDS, 0.5% sodium deoxycholate, 0.5% Igepal CA-630, protease inhibitor cocktail, and two units/µL of RNaseOUT Recombinant Ribonuclease Inhibitor), and then three times with 1 ml of PBS cell lysis buffer. .. The remaining sample was treated with an equal volume of 2 x Proteinase K solution (0.2 mg/ml proteinase K, 40 mM Tris–HCl pH 7.5, 5 mM EDTA, 33.4 ng/ml GlycoBlue (ThermoFisher Scientific AM9515), 0.2 mg/ml total yeast RNA (Sigma-Aldrich R6625)) for 30 min at 37°C to digest protein bound to the beads and release RNA.

    Fluorescence In Situ Hybridization:

    Article Title: Genome Structure and Thymic Expression of an Endogenous Retrovirus in Zebrafish
    Article Snippet: Both larval fish and paraffin sections were treated with 0.3% Triton X-100 for 15 min and with proteinase K (15 μg/ml) for 10 min, followed by incubation in acetylation solution (0.1 M triethanolamine [pH 8], 0.25% [vol/vol] acetic anhydride; Sigma) twice for 5 min each. .. For hybridization, samples were incubated with prehybridization buffer (4× SSC [600 mM NaCl, 60 mM sodium citrate; pH 7], 50% [vol/vol] formamide) at 37°C for 10 min, followed by incubation at 42°C for 16 h in hybridization buffer (50% [vol/vol] formamide, 4× SSC, 1× Denhardt solution [200 mg/liter each for Ficoll 400, polyvinylpyrrolidone, and bovine serum albumin], 100 μg of heparin/ml, 0.1% Tween 20, 1 mg of yeast Torula RNA [Sigma]/ml) containing fluorescein- or digoxigenin [DIG]-labeled RNA probe (0.2 μg/ml).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Millipore target grna
    Target Grna, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/target grna/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    target grna - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    96
    Millipore sacas9 mrna
    Sacas9 Mrna, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sacas9 mrna/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sacas9 mrna - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    Image Search Results