yeast torula rna  (Millipore)


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  • 99
    Name:
    Yeast Extract
    Description:

    Catalog Number:
    y4250
    Price:
    None
    Applications:
    Yeast extract is used as a bacteriological media component for a variety of microorganisms and molecular genetics applications.
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    Structured Review

    Millipore yeast torula rna
    Expression of ZFERV-related <t>RNA</t> in thymus. (A) Larval fish at 5 dpf were fixed and subjected to whole mount in situ hybridization with a <t>DIG-labeled</t> sense or antisense probe for the env gene, followed by colorimetric detection. (B and C) Contiguous sections (20 μm thick) of 3-month-old fish (right two panels) were subjected to in situ hybridization with probes as described in panel A. The section planes and main organs are indicated. Abbreviations: B, brain; E, esophagus; G, gill; H, heart; I, intestine; L, liver; M, muscle; T, thymus. The bilateral thymi hybridizing to the antisense probe are highlighted with arrows. (D) Sections (2 μm thick) of the thymus from 3-month-old fish were hybridized to fluorescein-labeled sense (left panel) or antisense (right panel) env probe, followed by fluorescence detection with the alkaline phosphatase-Fast Red system.

    https://www.bioz.com/result/yeast torula rna/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    yeast torula rna - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Genome Structure and Thymic Expression of an Endogenous Retrovirus in Zebrafish"

    Article Title: Genome Structure and Thymic Expression of an Endogenous Retrovirus in Zebrafish

    Journal: Journal of Virology

    doi: 10.1128/JVI.78.2.899-911.2004

    Expression of ZFERV-related RNA in thymus. (A) Larval fish at 5 dpf were fixed and subjected to whole mount in situ hybridization with a DIG-labeled sense or antisense probe for the env gene, followed by colorimetric detection. (B and C) Contiguous sections (20 μm thick) of 3-month-old fish (right two panels) were subjected to in situ hybridization with probes as described in panel A. The section planes and main organs are indicated. Abbreviations: B, brain; E, esophagus; G, gill; H, heart; I, intestine; L, liver; M, muscle; T, thymus. The bilateral thymi hybridizing to the antisense probe are highlighted with arrows. (D) Sections (2 μm thick) of the thymus from 3-month-old fish were hybridized to fluorescein-labeled sense (left panel) or antisense (right panel) env probe, followed by fluorescence detection with the alkaline phosphatase-Fast Red system.
    Figure Legend Snippet: Expression of ZFERV-related RNA in thymus. (A) Larval fish at 5 dpf were fixed and subjected to whole mount in situ hybridization with a DIG-labeled sense or antisense probe for the env gene, followed by colorimetric detection. (B and C) Contiguous sections (20 μm thick) of 3-month-old fish (right two panels) were subjected to in situ hybridization with probes as described in panel A. The section planes and main organs are indicated. Abbreviations: B, brain; E, esophagus; G, gill; H, heart; I, intestine; L, liver; M, muscle; T, thymus. The bilateral thymi hybridizing to the antisense probe are highlighted with arrows. (D) Sections (2 μm thick) of the thymus from 3-month-old fish were hybridized to fluorescein-labeled sense (left panel) or antisense (right panel) env probe, followed by fluorescence detection with the alkaline phosphatase-Fast Red system.

    Techniques Used: Expressing, Fluorescence In Situ Hybridization, In Situ Hybridization, Labeling, Fluorescence

    Related Articles

    Recombinant:

    Article Title: Two enzymes with redundant fructose bisphosphatase activity sustain gluconeogenesis and virulence in Mycobacterium tuberculosis
    Article Snippet: .. Reactions were performed in cuvettes with a 1 mL final reaction volume containing 20 mM Tris-HCl pH 7.7, 8 mM MgCl2 , 50 mM KCl, 1 mM NADP+ , 1 U/mL yeast glucose 6-phosphate dehydrogenase (Sigma-Aldrich), 2.5 U/mL yeast phosphoglucoisomerase (Sigma-Aldrich) and either 50 μg/mL total protein lysate or 0.05 μg/mL purified recombinant GPM2. .. Reactions were incubated at 30°C for 5 minutes before starting the reactions by adding fructose-1,6 bisphosphate.

    Article Title: In Vivo Function of Hsp90 Is Dependent on ATP Binding and ATP Hydrolysis
    Article Snippet: .. Yeast p23 protein (100 μg) with an NH2 -terminal T7 tag was added and the mixture was agitated at 30°C for 45 min in the prescence or absence of nucleotide and GA. After three washes with buffer Y containing 25 mM imidazole, Hsp82 protein was eluted from the resin with 500 mM imidazole in the same buffer, followed by SDS-PAGE, and then immunoblotting with an antibody against the T7 tag of recombinant yeast p23 (Novagen, Inc.). .. Miscellaneous Procedures SDS-PAGE was as described (Laemmli et al., 1970).

    Transformation Assay:

    Article Title: Lysine harvesting is an antioxidant strategy and triggers underground polyamine metabolism
    Article Snippet: .. The MAT ɑ wild-type strain of the S288c background BY4742 transformed with the plasmids pHLU or pHLUK, to repair auxotrophic markers , were grown in SM or synthetic complete (SC) media (0.56 g l-1 complete supplement mixture (MP Biomedicals) and 6.8 g l-1 yeast nitrogen base (Sigma-Aldrich)) supplemented with 2% glucose, with or without lysine. ..

    other:

    Article Title: Targeting RNA by Small Molecules: Comparative Structural and Thermodynamic Aspects of Aristololactam-?-D-glucoside and Daunomycin Binding to tRNAphe
    Article Snippet: Biochemicals tRNAPhe (yeast) and daunomycin were purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA).

    SDS Page:

    Article Title: In Vivo Function of Hsp90 Is Dependent on ATP Binding and ATP Hydrolysis
    Article Snippet: .. Yeast p23 protein (100 μg) with an NH2 -terminal T7 tag was added and the mixture was agitated at 30°C for 45 min in the prescence or absence of nucleotide and GA. After three washes with buffer Y containing 25 mM imidazole, Hsp82 protein was eluted from the resin with 500 mM imidazole in the same buffer, followed by SDS-PAGE, and then immunoblotting with an antibody against the T7 tag of recombinant yeast p23 (Novagen, Inc.). .. Miscellaneous Procedures SDS-PAGE was as described (Laemmli et al., 1970).

    Article Title: Deterioration of willow seeds during storage
    Article Snippet: .. Protein extracts from different harvest times were resolved on 12% SDS-PAGE gels containing herring sperm DNA (Biodynamics, Argentina) or torula yeast RNA (Sigma Aldrich, Merck, Darmstadt, Germany). .. For single-stranded DNase activity assays, DNA was boiled for 5 min prior to pouring in the gels.

    Purification:

    Article Title: Two enzymes with redundant fructose bisphosphatase activity sustain gluconeogenesis and virulence in Mycobacterium tuberculosis
    Article Snippet: .. Reactions were performed in cuvettes with a 1 mL final reaction volume containing 20 mM Tris-HCl pH 7.7, 8 mM MgCl2 , 50 mM KCl, 1 mM NADP+ , 1 U/mL yeast glucose 6-phosphate dehydrogenase (Sigma-Aldrich), 2.5 U/mL yeast phosphoglucoisomerase (Sigma-Aldrich) and either 50 μg/mL total protein lysate or 0.05 μg/mL purified recombinant GPM2. .. Reactions were incubated at 30°C for 5 minutes before starting the reactions by adding fructose-1,6 bisphosphate.

    Article Title: Interaction of Sulforaphane with DNA and RNA
    Article Snippet: .. Materials DNA sodium salt, and Baker’s yeast RNA sodium sulforaphane were purchased from Sigma Chemical (St. Louis, MO) and used without further purification. ..

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