yeast ribopure rna purification kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher yeast ribopure rna purification kit
    Yeast Ribopure Rna Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/yeast ribopure rna purification kit/product/Thermo Fisher
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    yeast ribopure rna purification kit - by Bioz Stars, 2020-04
    92/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: The Involvement of Mig1 from Xanthophyllomyces dendrorhous in Catabolic Repression: An Active Mechanism Contributing to the Regulation of Carotenoid Production
    Article Snippet: The general PCR protocol was: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and synthesis at 72°C for 3 min; and a final extension step at 72°C for 10 min. Total DNA was extracted from the yeasts X . dendrorhous and S . cerevisiae as previously described [ ], and total RNA was obtained from X . dendrorhous using the yeast RiboPure RNA Purification Kit (Ambion, Thermo Fisher Scientific Inc., Waltham, MA, USA) according to the manufacturer's recommendations. .. The AxyPrep Plasmid Miniprep Kit (Axygen, Corning Incorporated, Corning, NY, USA) were used for E . coli plasmid DNA extraction and purification.

    SYBR Green Assay:

    Article Title: Selective aggregation of the splicing factor Hsh155 suppresses splicing upon genotoxic stress
    Article Snippet: RNA isolation, cDNA preparation, and reverse transcription–quantitative PCR analysis Total RNA was isolated from log phase–grown cells treated with or without MMS and 2 h after MMS washout using the yeast RiboPure RNA Purification kit (Ambion). .. Reverse transcription–quantitative PCRs were performed and analyzed using SYBR green PCR Master Mix and a StepOnePlus Real-Time PCR system (Applied Biosystems). cDNA PCR products and reverse transcription–quantitative PCR transcripts were amplified using the primers in Table S5.

    Article Title: Selective defects in gene expression control genome instability in yeast splicing mutants
    Article Snippet: RNA isolation, cDNA preparation, and reverse transcription–quantitative PCR analysis Total RNA was isolated from 0.5–1 OD cell cultures shifted to 34°C for 3.5 h, using the yeast RiboPure RNA Purification kit (Ambion). cDNA (1 µg) was reverse transcribed using anchored-oligo(dT)18 primer and Transcriptor Reverse transcription (Roche). .. Reverse transcription–quantitative PCRs were performed and analyzed using SYBR green PCR Master Mix and a StepOnePlus Real-Time PCR system (Applied Biosystems).

    Amplification:

    Article Title: Selective aggregation of the splicing factor Hsh155 suppresses splicing upon genotoxic stress
    Article Snippet: RNA isolation, cDNA preparation, and reverse transcription–quantitative PCR analysis Total RNA was isolated from log phase–grown cells treated with or without MMS and 2 h after MMS washout using the yeast RiboPure RNA Purification kit (Ambion). .. Reverse transcription–quantitative PCRs were performed and analyzed using SYBR green PCR Master Mix and a StepOnePlus Real-Time PCR system (Applied Biosystems). cDNA PCR products and reverse transcription–quantitative PCR transcripts were amplified using the primers in Table S5.

    Agarose Gel Electrophoresis:

    Article Title: The Involvement of Mig1 from Xanthophyllomyces dendrorhous in Catabolic Repression: An Active Mechanism Contributing to the Regulation of Carotenoid Production
    Article Snippet: The general PCR protocol was: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and synthesis at 72°C for 3 min; and a final extension step at 72°C for 10 min. Total DNA was extracted from the yeasts X . dendrorhous and S . cerevisiae as previously described [ ], and total RNA was obtained from X . dendrorhous using the yeast RiboPure RNA Purification Kit (Ambion, Thermo Fisher Scientific Inc., Waltham, MA, USA) according to the manufacturer's recommendations. .. The integrity and concentration of genomic DNA, plasmids, RNA and PCR products were analyzed by performing 0.7–1% agarose gel electrophoresis in 1X TAE buffer (40 mM Tris-acetate, 1 mM EDTA mM, pH 8.0).

    Ligation:

    Article Title: The Involvement of Mig1 from Xanthophyllomyces dendrorhous in Catabolic Repression: An Active Mechanism Contributing to the Regulation of Carotenoid Production
    Article Snippet: Standard molecular biology procedures, nucleic acid extraction, PCR reactions, synthesis of cDNA and qPCR Standard procedures (PCR reactions, restriction enzyme digestions, ligation reactions and E . coli transformations) were performed according to standard protocols [ ]. .. The general PCR protocol was: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and synthesis at 72°C for 3 min; and a final extension step at 72°C for 10 min. Total DNA was extracted from the yeasts X . dendrorhous and S . cerevisiae as previously described [ ], and total RNA was obtained from X . dendrorhous using the yeast RiboPure RNA Purification Kit (Ambion, Thermo Fisher Scientific Inc., Waltham, MA, USA) according to the manufacturer's recommendations.

    Isolation:

    Article Title: Selective aggregation of the splicing factor Hsh155 suppresses splicing upon genotoxic stress
    Article Snippet: .. RNA isolation, cDNA preparation, and reverse transcription–quantitative PCR analysis Total RNA was isolated from log phase–grown cells treated with or without MMS and 2 h after MMS washout using the yeast RiboPure RNA Purification kit (Ambion). .. 1 µg of cDNA was reverse transcribed using anchored-oligo(dT)18 primer and Transcriptor Reverse transcription (Roche).

    Article Title: Selective defects in gene expression control genome instability in yeast splicing mutants
    Article Snippet: .. RNA isolation, cDNA preparation, and reverse transcription–quantitative PCR analysis Total RNA was isolated from 0.5–1 OD cell cultures shifted to 34°C for 3.5 h, using the yeast RiboPure RNA Purification kit (Ambion). cDNA (1 µg) was reverse transcribed using anchored-oligo(dT)18 primer and Transcriptor Reverse transcription (Roche). .. Reverse transcription–quantitative PCRs were performed and analyzed using SYBR green PCR Master Mix and a StepOnePlus Real-Time PCR system (Applied Biosystems).

    Purification:

    Article Title: Selective aggregation of the splicing factor Hsh155 suppresses splicing upon genotoxic stress
    Article Snippet: .. RNA isolation, cDNA preparation, and reverse transcription–quantitative PCR analysis Total RNA was isolated from log phase–grown cells treated with or without MMS and 2 h after MMS washout using the yeast RiboPure RNA Purification kit (Ambion). .. 1 µg of cDNA was reverse transcribed using anchored-oligo(dT)18 primer and Transcriptor Reverse transcription (Roche).

    Article Title: Selective defects in gene expression control genome instability in yeast splicing mutants
    Article Snippet: .. RNA isolation, cDNA preparation, and reverse transcription–quantitative PCR analysis Total RNA was isolated from 0.5–1 OD cell cultures shifted to 34°C for 3.5 h, using the yeast RiboPure RNA Purification kit (Ambion). cDNA (1 µg) was reverse transcribed using anchored-oligo(dT)18 primer and Transcriptor Reverse transcription (Roche). .. Reverse transcription–quantitative PCRs were performed and analyzed using SYBR green PCR Master Mix and a StepOnePlus Real-Time PCR system (Applied Biosystems).

    Article Title: The Involvement of Mig1 from Xanthophyllomyces dendrorhous in Catabolic Repression: An Active Mechanism Contributing to the Regulation of Carotenoid Production
    Article Snippet: .. The general PCR protocol was: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and synthesis at 72°C for 3 min; and a final extension step at 72°C for 10 min. Total DNA was extracted from the yeasts X . dendrorhous and S . cerevisiae as previously described [ ], and total RNA was obtained from X . dendrorhous using the yeast RiboPure RNA Purification Kit (Ambion, Thermo Fisher Scientific Inc., Waltham, MA, USA) according to the manufacturer's recommendations. .. The AxyPrep Plasmid Miniprep Kit (Axygen, Corning Incorporated, Corning, NY, USA) were used for E . coli plasmid DNA extraction and purification.

    Real-time Polymerase Chain Reaction:

    Article Title: Selective aggregation of the splicing factor Hsh155 suppresses splicing upon genotoxic stress
    Article Snippet: RNA isolation, cDNA preparation, and reverse transcription–quantitative PCR analysis Total RNA was isolated from log phase–grown cells treated with or without MMS and 2 h after MMS washout using the yeast RiboPure RNA Purification kit (Ambion). .. Reverse transcription–quantitative PCRs were performed and analyzed using SYBR green PCR Master Mix and a StepOnePlus Real-Time PCR system (Applied Biosystems). cDNA PCR products and reverse transcription–quantitative PCR transcripts were amplified using the primers in Table S5.

    Article Title: Selective defects in gene expression control genome instability in yeast splicing mutants
    Article Snippet: RNA isolation, cDNA preparation, and reverse transcription–quantitative PCR analysis Total RNA was isolated from 0.5–1 OD cell cultures shifted to 34°C for 3.5 h, using the yeast RiboPure RNA Purification kit (Ambion). cDNA (1 µg) was reverse transcribed using anchored-oligo(dT)18 primer and Transcriptor Reverse transcription (Roche). .. Reverse transcription–quantitative PCRs were performed and analyzed using SYBR green PCR Master Mix and a StepOnePlus Real-Time PCR system (Applied Biosystems).

    Article Title: The Involvement of Mig1 from Xanthophyllomyces dendrorhous in Catabolic Repression: An Active Mechanism Contributing to the Regulation of Carotenoid Production
    Article Snippet: Paragraph title: Standard molecular biology procedures, nucleic acid extraction, PCR reactions, synthesis of cDNA and qPCR ... The general PCR protocol was: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and synthesis at 72°C for 3 min; and a final extension step at 72°C for 10 min. Total DNA was extracted from the yeasts X . dendrorhous and S . cerevisiae as previously described [ ], and total RNA was obtained from X . dendrorhous using the yeast RiboPure RNA Purification Kit (Ambion, Thermo Fisher Scientific Inc., Waltham, MA, USA) according to the manufacturer's recommendations.

    Concentration Assay:

    Article Title: The Involvement of Mig1 from Xanthophyllomyces dendrorhous in Catabolic Repression: An Active Mechanism Contributing to the Regulation of Carotenoid Production
    Article Snippet: The general PCR protocol was: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and synthesis at 72°C for 3 min; and a final extension step at 72°C for 10 min. Total DNA was extracted from the yeasts X . dendrorhous and S . cerevisiae as previously described [ ], and total RNA was obtained from X . dendrorhous using the yeast RiboPure RNA Purification Kit (Ambion, Thermo Fisher Scientific Inc., Waltham, MA, USA) according to the manufacturer's recommendations. .. The integrity and concentration of genomic DNA, plasmids, RNA and PCR products were analyzed by performing 0.7–1% agarose gel electrophoresis in 1X TAE buffer (40 mM Tris-acetate, 1 mM EDTA mM, pH 8.0).

    Polymerase Chain Reaction:

    Article Title: Selective aggregation of the splicing factor Hsh155 suppresses splicing upon genotoxic stress
    Article Snippet: .. RNA isolation, cDNA preparation, and reverse transcription–quantitative PCR analysis Total RNA was isolated from log phase–grown cells treated with or without MMS and 2 h after MMS washout using the yeast RiboPure RNA Purification kit (Ambion). .. 1 µg of cDNA was reverse transcribed using anchored-oligo(dT)18 primer and Transcriptor Reverse transcription (Roche).

    Article Title: Selective defects in gene expression control genome instability in yeast splicing mutants
    Article Snippet: .. RNA isolation, cDNA preparation, and reverse transcription–quantitative PCR analysis Total RNA was isolated from 0.5–1 OD cell cultures shifted to 34°C for 3.5 h, using the yeast RiboPure RNA Purification kit (Ambion). cDNA (1 µg) was reverse transcribed using anchored-oligo(dT)18 primer and Transcriptor Reverse transcription (Roche). .. Reverse transcription–quantitative PCRs were performed and analyzed using SYBR green PCR Master Mix and a StepOnePlus Real-Time PCR system (Applied Biosystems).

    Article Title: The Involvement of Mig1 from Xanthophyllomyces dendrorhous in Catabolic Repression: An Active Mechanism Contributing to the Regulation of Carotenoid Production
    Article Snippet: .. The general PCR protocol was: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and synthesis at 72°C for 3 min; and a final extension step at 72°C for 10 min. Total DNA was extracted from the yeasts X . dendrorhous and S . cerevisiae as previously described [ ], and total RNA was obtained from X . dendrorhous using the yeast RiboPure RNA Purification Kit (Ambion, Thermo Fisher Scientific Inc., Waltham, MA, USA) according to the manufacturer's recommendations. .. The AxyPrep Plasmid Miniprep Kit (Axygen, Corning Incorporated, Corning, NY, USA) were used for E . coli plasmid DNA extraction and purification.

    Staining:

    Article Title: The Involvement of Mig1 from Xanthophyllomyces dendrorhous in Catabolic Repression: An Active Mechanism Contributing to the Regulation of Carotenoid Production
    Article Snippet: The general PCR protocol was: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and synthesis at 72°C for 3 min; and a final extension step at 72°C for 10 min. Total DNA was extracted from the yeasts X . dendrorhous and S . cerevisiae as previously described [ ], and total RNA was obtained from X . dendrorhous using the yeast RiboPure RNA Purification Kit (Ambion, Thermo Fisher Scientific Inc., Waltham, MA, USA) according to the manufacturer's recommendations. .. Gels were stained with 0.5 μg/ml ethidium bromide and visualized under UV light.

    Plasmid Preparation:

    Article Title: The Involvement of Mig1 from Xanthophyllomyces dendrorhous in Catabolic Repression: An Active Mechanism Contributing to the Regulation of Carotenoid Production
    Article Snippet: The general PCR protocol was: initial denaturation at 95°C for 3 min; 35 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and synthesis at 72°C for 3 min; and a final extension step at 72°C for 10 min. Total DNA was extracted from the yeasts X . dendrorhous and S . cerevisiae as previously described [ ], and total RNA was obtained from X . dendrorhous using the yeast RiboPure RNA Purification Kit (Ambion, Thermo Fisher Scientific Inc., Waltham, MA, USA) according to the manufacturer's recommendations. .. The AxyPrep Plasmid Miniprep Kit (Axygen, Corning Incorporated, Corning, NY, USA) were used for E . coli plasmid DNA extraction and purification.

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  • 99
    Thermo Fisher ribopure yeast rna purification kit
    Ribopure Yeast Rna Purification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribopure yeast rna purification kit/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    ribopure yeast rna purification kit - by Bioz Stars, 2020-04
    99/100 stars
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