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Agilent technologies xl1 blue e scheri chia coli competent cells
Xl1 Blue E Scheri Chia Coli Competent Cells, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xl1 blue e scheri chia coli competent cells/product/Agilent technologies
Average 80 stars, based on 1 article reviews
Price from $9.99 to $1999.99
xl1 blue e scheri chia coli competent cells - by Bioz Stars, 2020-04
80/100 stars

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Clone Assay:

Article Title: Frequent cross-species transmissions of foamy virus between domestic and wild felids
Article Snippet: .. Purified DNA was then cloned using pJET 1.2 blunt vector using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific, USA) and XL1-Blue E scheri chia coli competent cells (Agilent technologies, USA). .. Plasmids were isolated using DNA-spin Plasmid Purification Kit (iNtRON Biotechnology, Korea) and subsequently Sanger sequenced using primer walking at Quintarabio in San Francisco, CA.

Agarose Gel Electrophoresis:

Article Title: Frequent cross-species transmissions of foamy virus between domestic and wild felids
Article Snippet: PCR products were resolved on a 0.7 per cent agarose gel using electrophoresis for 30 min at 110 V. Bands of the correct size were excised from the gel and purified using a MEGAquick-spin™ Total Fragment DNA Purification Kit (iNtRON Biotechnology, Korea). .. Purified DNA was then cloned using pJET 1.2 blunt vector using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific, USA) and XL1-Blue E scheri chia coli competent cells (Agilent technologies, USA).

Polymerase Chain Reaction:

Article Title: Frequent cross-species transmissions of foamy virus between domestic and wild felids
Article Snippet: .. Purified DNA was then cloned using pJET 1.2 blunt vector using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific, USA) and XL1-Blue E scheri chia coli competent cells (Agilent technologies, USA). .. Plasmids were isolated using DNA-spin Plasmid Purification Kit (iNtRON Biotechnology, Korea) and subsequently Sanger sequenced using primer walking at Quintarabio in San Francisco, CA.

Isolation:

Article Title: Frequent cross-species transmissions of foamy virus between domestic and wild felids
Article Snippet: Purified DNA was then cloned using pJET 1.2 blunt vector using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific, USA) and XL1-Blue E scheri chia coli competent cells (Agilent technologies, USA). .. Plasmids were isolated using DNA-spin Plasmid Purification Kit (iNtRON Biotechnology, Korea) and subsequently Sanger sequenced using primer walking at Quintarabio in San Francisco, CA.

Chromosome Walking:

Article Title: Frequent cross-species transmissions of foamy virus between domestic and wild felids
Article Snippet: Purified DNA was then cloned using pJET 1.2 blunt vector using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific, USA) and XL1-Blue E scheri chia coli competent cells (Agilent technologies, USA). .. Plasmids were isolated using DNA-spin Plasmid Purification Kit (iNtRON Biotechnology, Korea) and subsequently Sanger sequenced using primer walking at Quintarabio in San Francisco, CA.

Purification:

Article Title: Frequent cross-species transmissions of foamy virus between domestic and wild felids
Article Snippet: .. Purified DNA was then cloned using pJET 1.2 blunt vector using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific, USA) and XL1-Blue E scheri chia coli competent cells (Agilent technologies, USA). .. Plasmids were isolated using DNA-spin Plasmid Purification Kit (iNtRON Biotechnology, Korea) and subsequently Sanger sequenced using primer walking at Quintarabio in San Francisco, CA.

Electrophoresis:

Article Title: Frequent cross-species transmissions of foamy virus between domestic and wild felids
Article Snippet: PCR products were resolved on a 0.7 per cent agarose gel using electrophoresis for 30 min at 110 V. Bands of the correct size were excised from the gel and purified using a MEGAquick-spin™ Total Fragment DNA Purification Kit (iNtRON Biotechnology, Korea). .. Purified DNA was then cloned using pJET 1.2 blunt vector using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific, USA) and XL1-Blue E scheri chia coli competent cells (Agilent technologies, USA).

Nested PCR:

Article Title: Frequent cross-species transmissions of foamy virus between domestic and wild felids
Article Snippet: Nested PCR was used to recover proviral sequences for Sanger sequencing as follows: First Round 5 µl Kapa Hotstart Hifi polymerase (Kapa Biosystems, USA), 2 µl H2 O, 1 µl of both the forward and reverse primer ( ) and 2 µl of DNA. .. Purified DNA was then cloned using pJET 1.2 blunt vector using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific, USA) and XL1-Blue E scheri chia coli competent cells (Agilent technologies, USA).

DNA Purification:

Article Title: Frequent cross-species transmissions of foamy virus between domestic and wild felids
Article Snippet: PCR products were resolved on a 0.7 per cent agarose gel using electrophoresis for 30 min at 110 V. Bands of the correct size were excised from the gel and purified using a MEGAquick-spin™ Total Fragment DNA Purification Kit (iNtRON Biotechnology, Korea). .. Purified DNA was then cloned using pJET 1.2 blunt vector using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific, USA) and XL1-Blue E scheri chia coli competent cells (Agilent technologies, USA).

Sequencing:

Article Title: Frequent cross-species transmissions of foamy virus between domestic and wild felids
Article Snippet: Nested PCR was used to recover proviral sequences for Sanger sequencing as follows: First Round 5 µl Kapa Hotstart Hifi polymerase (Kapa Biosystems, USA), 2 µl H2 O, 1 µl of both the forward and reverse primer ( ) and 2 µl of DNA. .. Purified DNA was then cloned using pJET 1.2 blunt vector using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific, USA) and XL1-Blue E scheri chia coli competent cells (Agilent technologies, USA).

Plasmid Preparation:

Article Title: Frequent cross-species transmissions of foamy virus between domestic and wild felids
Article Snippet: .. Purified DNA was then cloned using pJET 1.2 blunt vector using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific, USA) and XL1-Blue E scheri chia coli competent cells (Agilent technologies, USA). .. Plasmids were isolated using DNA-spin Plasmid Purification Kit (iNtRON Biotechnology, Korea) and subsequently Sanger sequenced using primer walking at Quintarabio in San Francisco, CA.

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    Agilent technologies xl1 blue e scheri chia coli competent cells
    Xl1 Blue E Scheri Chia Coli Competent Cells, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xl1 blue e scheri chia coli competent cells/product/Agilent technologies
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xl1 blue e scheri chia coli competent cells - by Bioz Stars, 2020-04
    80/100 stars
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