Structured Review

Millipore xhoi
Xhoi, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xhoi/product/Millipore
Average 97 stars, based on 331 article reviews
Price from $9.99 to $1999.99
xhoi - by Bioz Stars, 2020-04
97/100 stars

Images

Related Articles

Clone Assay:

Article Title: Oligomerization of the carboxyl terminal domain of the human coronavirus 229E nucleocapsid protein.
Article Snippet: .. The PCR products were digested with NdeI and XhoI, and the DNA fragments were cloned into pET21b (Novagen) using T4 ligase (NEB). .. The induction of protein expression was initiated by adding IPTG to 1 mM followed by incubation at 37°C for 6 h. After harvesting the bacteria by centrifugation (6000 rpm, 30 min, 4°C), the bacterial cells were lysed with lysis buffer (50 mM Tris-HCl, pH 7.3, 150 mM NaCl, and 15 mM imidazole).

Article Title: Crystal structure of the papain-like protease of MERS coronavirus reveals unusual, potentially druggable active-site features.
Article Snippet: The resulting PCR product was digested with restriction enzymes NheI and XhoI for ligation into pET-28a (Novagen). .. Cloning was designed to include an N-terminal hexahistidine (His 6 ) tag and a thrombin cleavage site.

Article Title: A new subunit vaccine based on nucleoprotein nanoparticles confers partial clinical and virological protection in calves against bovine respiratory syncytial virus.
Article Snippet: .. Plasmid constructions The amplified full-length cDNA coding for BRSV N protein was digested subsequently by NcoI and XhoI and cloned into pET-28a(+) vector (Novagen, Merck Chemicals products, Germany). .. The PCT coding for amino acid residues 161-241 of BRSV P protein was digested subsequently by BamHI and XhoI and inserted into the pGEX-4T3 expression vector (Pharmacia, France).

Article Title: Binding characterization of determinants in porcine aminopeptidase N, the cellular receptor for transmissible gastroenteritis virus.
Article Snippet: .. The amplicons were inserted into the multiple cloning sites BamHI and XhoI of pET-30a(+) vector (Novagen, Germany) using standard molecular cloning techniques. .. Expression and purification of pAPN in E. coli The above-mentioned recombinant plasmids were transformed into E. coli BL21(DE3)pLysS (Novagen, Germany) and the transformed cells were cultured in Luria bertani (LB) medium containing Ampicillin (50 g/ml) at 37 • C with shaking until the optical density (OD) of the culture at 600 nm reached 0.5.

Centrifugation:

Article Title: Oligomerization of the carboxyl terminal domain of the human coronavirus 229E nucleocapsid protein.
Article Snippet: The PCR products were digested with NdeI and XhoI, and the DNA fragments were cloned into pET21b (Novagen) using T4 ligase (NEB). .. The induction of protein expression was initiated by adding IPTG to 1 mM followed by incubation at 37°C for 6 h. After harvesting the bacteria by centrifugation (6000 rpm, 30 min, 4°C), the bacterial cells were lysed with lysis buffer (50 mM Tris-HCl, pH 7.3, 150 mM NaCl, and 15 mM imidazole).

Article Title: Characterization of trans- and cis-cleavage activity of the SARS coronavirus 3CLpro protease: basis for the in vitro screening of anti-SARS drugs.
Article Snippet: Each RT-PCR product was digested with EcoRI and XhoI, and then ligated into the EcoRI/XhoI cleavage sites of the pET24a vector (Novagen) and expressed as a histidine tag fusion protein. .. The supernatant was purified with the HisTrap Kit and buffer kit (Amersham) after centrifugation (10 000 Â g for 20 min).

Amplification:

Article Title: Oligomerization of the carboxyl terminal domain of the human coronavirus 229E nucleocapsid protein.
Article Snippet: To generate the truncated forms of the recombinant N proteins, the N protein gene was amplified by polymerase chain reaction (PCR) from the plasmid pGENT using various primers. .. The PCR products were digested with NdeI and XhoI, and the DNA fragments were cloned into pET21b (Novagen) using T4 ligase (NEB).

Article Title: Crystal structure of the papain-like protease of MERS coronavirus reveals unusual, potentially druggable active-site features.
Article Snippet: A gene coding for the PL pro was amplified by the polymerase chain reaction (PCR) using the forward primer 5 0 -CTAGCTAGCcagttaacaatcgaagtcttagtg-3 0 and the reverse primer 5 0 -CCGCTCGAGttaatcgctactgtatttttggccggg-3 0 . .. The resulting PCR product was digested with restriction enzymes NheI and XhoI for ligation into pET-28a (Novagen).

Article Title: A new subunit vaccine based on nucleoprotein nanoparticles confers partial clinical and virological protection in calves against bovine respiratory syncytial virus.
Article Snippet: .. Plasmid constructions The amplified full-length cDNA coding for BRSV N protein was digested subsequently by NcoI and XhoI and cloned into pET-28a(+) vector (Novagen, Merck Chemicals products, Germany). .. The PCT coding for amino acid residues 161-241 of BRSV P protein was digested subsequently by BamHI and XhoI and inserted into the pGEX-4T3 expression vector (Pharmacia, France).

Article Title: Characterization of trans- and cis-cleavage activity of the SARS coronavirus 3CLpro protease: basis for the in vitro screening of anti-SARS drugs.
Article Snippet: Construction, expression, and purification of SARS-CoV 3CL pro The 3CL pro gene located within the nucleotides 9985-10 902 of the SARS-CoV TW1 strain genome (GenBank Accession No. AY291451) [14] was amplified using the reverse-transcriptase polymerase chain reaction (RT-PCR) with specific primers 5 0 -CCCGGATCCAGTG-GTTTTAGGAAAATGGCATTC-3 0 and 5 0 -GGTGCTCGAGTTGG-AAGGTAACACCAGAGCATTG-3 0 . .. Each RT-PCR product was digested with EcoRI and XhoI, and then ligated into the EcoRI/XhoI cleavage sites of the pET24a vector (Novagen) and expressed as a histidine tag fusion protein.

Article Title: A trimeric DNA polymerase complex increases the native replication processivity
Article Snippet: Sso Dpo1 was amplified from genomic S. solfataricus P2 (ATCC, Manassas, VA.) using Pfx 50 polymerase (Invitrogen, Carlsbad, CA). .. Specific restriction sites AseI and XhoI contained in the primers were used to clone Sso Dpo1 into pET30a digested with NdeI and XhoI (Novagen) to include a C terminal His tag.

Article Title: Cefoperazone induces esterase B expression by EstR and esterase B enhances cefoperazone activity at the periplasm.
Article Snippet: To construct histidine tagged PhoA, the gene was amplified from E. coli K-12 chromosome using primers BT7726 and BT 7727 producing 1415 bp fragment. .. The fragment was digested with XhoI before ligating with pETBlue2 Novagen which was digested with EcoRV and XhoI to obtain pETphoA before introducing into E. coli.

Positive Control:

Article Title: Cefoperazone induces esterase B expression by EstR and esterase B enhances cefoperazone activity at the periplasm.
Article Snippet: Construction of periplasmic PhoA (pET-phoA) An E. coli alkaline phosphatase encoded by phoA (P00634) was used as the positive control of periplasmic protein in this study. .. The fragment was digested with XhoI before ligating with pETBlue2 Novagen which was digested with EcoRV and XhoI to obtain pETphoA before introducing into E. coli.

Polymerase Chain Reaction:

Article Title: Oligomerization of the carboxyl terminal domain of the human coronavirus 229E nucleocapsid protein.
Article Snippet: .. The PCR products were digested with NdeI and XhoI, and the DNA fragments were cloned into pET21b (Novagen) using T4 ligase (NEB). .. The induction of protein expression was initiated by adding IPTG to 1 mM followed by incubation at 37°C for 6 h. After harvesting the bacteria by centrifugation (6000 rpm, 30 min, 4°C), the bacterial cells were lysed with lysis buffer (50 mM Tris-HCl, pH 7.3, 150 mM NaCl, and 15 mM imidazole).

Article Title: Crystal structure of the papain-like protease of MERS coronavirus reveals unusual, potentially druggable active-site features.
Article Snippet: .. The resulting PCR product was digested with restriction enzymes NheI and XhoI for ligation into pET-28a (Novagen). .. Cloning was designed to include an N-terminal hexahistidine (His 6 ) tag and a thrombin cleavage site.

Article Title: A new subunit vaccine based on nucleoprotein nanoparticles confers partial clinical and virological protection in calves against bovine respiratory syncytial virus.
Article Snippet: The cDNAs were amplified by PCR with high fidelity PfuTurbo Polymerase (5U, Stratagene, Agilent Technologies, France) and 100 ng of the following primers: N-A2G+: 5 -GAGGAGCCATGGCTCTTAGCAAGGTCAAACTAAATG-3 ; N-A2G−: 5 -GAGGAGCTCGAGTCACAATTCCACATCATTATCTTTGG-3 ; P-A2G+: 5 -GAGGGATCCATGGCTGCTCGTGATGGTATAAGAGATG-CCATG-3 ; P-A2G−: 5 -GAGGAGCTCGAGTCAGAAATCTTCAAGTGATAGATCA-TTGTC-3 . .. Plasmid constructions The amplified full-length cDNA coding for BRSV N protein was digested subsequently by NcoI and XhoI and cloned into pET-28a(+) vector (Novagen, Merck Chemicals products, Germany).

Article Title: Characterization of trans- and cis-cleavage activity of the SARS coronavirus 3CLpro protease: basis for the in vitro screening of anti-SARS drugs.
Article Snippet: Construction, expression, and purification of SARS-CoV 3CL pro The 3CL pro gene located within the nucleotides 9985-10 902 of the SARS-CoV TW1 strain genome (GenBank Accession No. AY291451) [14] was amplified using the reverse-transcriptase polymerase chain reaction (RT-PCR) with specific primers 5 0 -CCCGGATCCAGTG-GTTTTAGGAAAATGGCATTC-3 0 and 5 0 -GGTGCTCGAGTTGG-AAGGTAACACCAGAGCATTG-3 0 . .. Each RT-PCR product was digested with EcoRI and XhoI, and then ligated into the EcoRI/XhoI cleavage sites of the pET24a vector (Novagen) and expressed as a histidine tag fusion protein.

Article Title: A trimeric DNA polymerase complex increases the native replication processivity
Article Snippet: Initial ligation of the PCR product into pGMET (Promega, Madison, WI) was performed using standard T-cloning. .. Specific restriction sites AseI and XhoI contained in the primers were used to clone Sso Dpo1 into pET30a digested with NdeI and XhoI (Novagen) to include a C terminal His tag.

Article Title: Binding characterization of determinants in porcine aminopeptidase N, the cellular receptor for transmissible gastroenteritis virus.
Article Snippet: The PCR profile included 95 • C for 5 min, 30 cycles of 95 • C for 5 min, 63.2 • C for 30 s, 72 • C for 1.5 min and followed by a final extension of 72 • C for 10 min. .. The amplicons were inserted into the multiple cloning sites BamHI and XhoI of pET-30a(+) vector (Novagen, Germany) using standard molecular cloning techniques.

Construct:

Article Title: Cefoperazone induces esterase B expression by EstR and esterase B enhances cefoperazone activity at the periplasm.
Article Snippet: To construct histidine tagged PhoA, the gene was amplified from E. coli K-12 chromosome using primers BT7726 and BT 7727 producing 1415 bp fragment. .. The fragment was digested with XhoI before ligating with pETBlue2 Novagen which was digested with EcoRV and XhoI to obtain pETphoA before introducing into E. coli.

Incubation:

Article Title: Oligomerization of the carboxyl terminal domain of the human coronavirus 229E nucleocapsid protein.
Article Snippet: The PCR products were digested with NdeI and XhoI, and the DNA fragments were cloned into pET21b (Novagen) using T4 ligase (NEB). .. The induction of protein expression was initiated by adding IPTG to 1 mM followed by incubation at 37°C for 6 h. After harvesting the bacteria by centrifugation (6000 rpm, 30 min, 4°C), the bacterial cells were lysed with lysis buffer (50 mM Tris-HCl, pH 7.3, 150 mM NaCl, and 15 mM imidazole).

Cell Culture:

Article Title: Characterization of trans- and cis-cleavage activity of the SARS coronavirus 3CLpro protease: basis for the in vitro screening of anti-SARS drugs.
Article Snippet: Each RT-PCR product was digested with EcoRI and XhoI, and then ligated into the EcoRI/XhoI cleavage sites of the pET24a vector (Novagen) and expressed as a histidine tag fusion protein. .. The Escherichia coli strain BL21(DE3) was transformed with the resulting plasmid, pET24a-3CL pro , then cultured in LB medium in the presence of 100 Â g of kanamycin per ml at 37°C.

Expressing:

Article Title: Oligomerization of the carboxyl terminal domain of the human coronavirus 229E nucleocapsid protein.
Article Snippet: Paragraph title: Expression and purification of the full-length and truncated N proteins ... The PCR products were digested with NdeI and XhoI, and the DNA fragments were cloned into pET21b (Novagen) using T4 ligase (NEB).

Article Title: A new subunit vaccine based on nucleoprotein nanoparticles confers partial clinical and virological protection in calves against bovine respiratory syncytial virus.
Article Snippet: Plasmid constructions The amplified full-length cDNA coding for BRSV N protein was digested subsequently by NcoI and XhoI and cloned into pET-28a(+) vector (Novagen, Merck Chemicals products, Germany). .. The PCT coding for amino acid residues 161-241 of BRSV P protein was digested subsequently by BamHI and XhoI and inserted into the pGEX-4T3 expression vector (Pharmacia, France).

Article Title: Characterization of trans- and cis-cleavage activity of the SARS coronavirus 3CLpro protease: basis for the in vitro screening of anti-SARS drugs.
Article Snippet: Paragraph title: Construction, expression, and purification of SARS-CoV 3CL pro ... Each RT-PCR product was digested with EcoRI and XhoI, and then ligated into the EcoRI/XhoI cleavage sites of the pET24a vector (Novagen) and expressed as a histidine tag fusion protein.

Transformation Assay:

Article Title: Crystal structure of the papain-like protease of MERS coronavirus reveals unusual, potentially druggable active-site features.
Article Snippet: The resulting PCR product was digested with restriction enzymes NheI and XhoI for ligation into pET-28a (Novagen). .. Transformed cells were grown at 37°C overnight in LB medium, supplemented with kanamycin (50 lg/mL) and chloramphenicol (34 lg/mL).

Article Title: Characterization of trans- and cis-cleavage activity of the SARS coronavirus 3CLpro protease: basis for the in vitro screening of anti-SARS drugs.
Article Snippet: Each RT-PCR product was digested with EcoRI and XhoI, and then ligated into the EcoRI/XhoI cleavage sites of the pET24a vector (Novagen) and expressed as a histidine tag fusion protein. .. The Escherichia coli strain BL21(DE3) was transformed with the resulting plasmid, pET24a-3CL pro , then cultured in LB medium in the presence of 100 Â g of kanamycin per ml at 37°C.

Ligation:

Article Title: Crystal structure of the papain-like protease of MERS coronavirus reveals unusual, potentially druggable active-site features.
Article Snippet: .. The resulting PCR product was digested with restriction enzymes NheI and XhoI for ligation into pET-28a (Novagen). .. Cloning was designed to include an N-terminal hexahistidine (His 6 ) tag and a thrombin cleavage site.

Article Title: A trimeric DNA polymerase complex increases the native replication processivity
Article Snippet: Initial ligation of the PCR product into pGMET (Promega, Madison, WI) was performed using standard T-cloning. .. Specific restriction sites AseI and XhoI contained in the primers were used to clone Sso Dpo1 into pET30a digested with NdeI and XhoI (Novagen) to include a C terminal His tag.

Infection:

Article Title: A new subunit vaccine based on nucleoprotein nanoparticles confers partial clinical and virological protection in calves against bovine respiratory syncytial virus.
Article Snippet: Randomprimed cDNA synthesis was done using SuperscriptII (GIBCO, Invitrogen Life Science, France) and 1 g of total cytoplasmic RNA isolated from bovine Turbinate cells infected with the A2Gelfi strain of BRSV [23, 24] . .. Plasmid constructions The amplified full-length cDNA coding for BRSV N protein was digested subsequently by NcoI and XhoI and cloned into pET-28a(+) vector (Novagen, Merck Chemicals products, Germany).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Characterization of trans- and cis-cleavage activity of the SARS coronavirus 3CLpro protease: basis for the in vitro screening of anti-SARS drugs.
Article Snippet: .. Each RT-PCR product was digested with EcoRI and XhoI, and then ligated into the EcoRI/XhoI cleavage sites of the pET24a vector (Novagen) and expressed as a histidine tag fusion protein. .. The Escherichia coli strain BL21(DE3) was transformed with the resulting plasmid, pET24a-3CL pro , then cultured in LB medium in the presence of 100 Â g of kanamycin per ml at 37°C.

Recombinant:

Article Title: Oligomerization of the carboxyl terminal domain of the human coronavirus 229E nucleocapsid protein.
Article Snippet: To generate the truncated forms of the recombinant N proteins, the N protein gene was amplified by polymerase chain reaction (PCR) from the plasmid pGENT using various primers. .. The PCR products were digested with NdeI and XhoI, and the DNA fragments were cloned into pET21b (Novagen) using T4 ligase (NEB).

Article Title: Crystal structure of the papain-like protease of MERS coronavirus reveals unusual, potentially druggable active-site features.
Article Snippet: Paragraph title: Recombinant production of MERS-CoV papain-like protease (PL pro ) ... The resulting PCR product was digested with restriction enzymes NheI and XhoI for ligation into pET-28a (Novagen).

Article Title: Binding characterization of determinants in porcine aminopeptidase N, the cellular receptor for transmissible gastroenteritis virus.
Article Snippet: Paragraph title: Construction of recombinant plasmids ... The amplicons were inserted into the multiple cloning sites BamHI and XhoI of pET-30a(+) vector (Novagen, Germany) using standard molecular cloning techniques.

Molecular Cloning:

Article Title: Binding characterization of determinants in porcine aminopeptidase N, the cellular receptor for transmissible gastroenteritis virus.
Article Snippet: .. The amplicons were inserted into the multiple cloning sites BamHI and XhoI of pET-30a(+) vector (Novagen, Germany) using standard molecular cloning techniques. .. Expression and purification of pAPN in E. coli The above-mentioned recombinant plasmids were transformed into E. coli BL21(DE3)pLysS (Novagen, Germany) and the transformed cells were cultured in Luria bertani (LB) medium containing Ampicillin (50 g/ml) at 37 • C with shaking until the optical density (OD) of the culture at 600 nm reached 0.5.

Mutagenesis:

Article Title: A trimeric DNA polymerase complex increases the native replication processivity
Article Snippet: Standard QuikChange protocol (Stratagene, La Jolla, CA) was used to create an exonuclease mutant of SsoDpo1 (D231A/D318A). .. Specific restriction sites AseI and XhoI contained in the primers were used to clone Sso Dpo1 into pET30a digested with NdeI and XhoI (Novagen) to include a C terminal His tag.

Isolation:

Article Title: A new subunit vaccine based on nucleoprotein nanoparticles confers partial clinical and virological protection in calves against bovine respiratory syncytial virus.
Article Snippet: Randomprimed cDNA synthesis was done using SuperscriptII (GIBCO, Invitrogen Life Science, France) and 1 g of total cytoplasmic RNA isolated from bovine Turbinate cells infected with the A2Gelfi strain of BRSV [23, 24] . .. Plasmid constructions The amplified full-length cDNA coding for BRSV N protein was digested subsequently by NcoI and XhoI and cloned into pET-28a(+) vector (Novagen, Merck Chemicals products, Germany).

Purification:

Article Title: Oligomerization of the carboxyl terminal domain of the human coronavirus 229E nucleocapsid protein.
Article Snippet: Paragraph title: Expression and purification of the full-length and truncated N proteins ... The PCR products were digested with NdeI and XhoI, and the DNA fragments were cloned into pET21b (Novagen) using T4 ligase (NEB).

Article Title: Characterization of trans- and cis-cleavage activity of the SARS coronavirus 3CLpro protease: basis for the in vitro screening of anti-SARS drugs.
Article Snippet: Paragraph title: Construction, expression, and purification of SARS-CoV 3CL pro ... Each RT-PCR product was digested with EcoRI and XhoI, and then ligated into the EcoRI/XhoI cleavage sites of the pET24a vector (Novagen) and expressed as a histidine tag fusion protein.

Sequencing:

Article Title: Binding characterization of determinants in porcine aminopeptidase N, the cellular receptor for transmissible gastroenteritis virus.
Article Snippet: Five pairs of primers were used to amplify five gene fragments encoding a signal peptide sequence-deleted pAPN and four truncated pAPN ( Table 1 ). .. The amplicons were inserted into the multiple cloning sites BamHI and XhoI of pET-30a(+) vector (Novagen, Germany) using standard molecular cloning techniques.

Plasmid Preparation:

Article Title: Oligomerization of the carboxyl terminal domain of the human coronavirus 229E nucleocapsid protein.
Article Snippet: To generate the truncated forms of the recombinant N proteins, the N protein gene was amplified by polymerase chain reaction (PCR) from the plasmid pGENT using various primers. .. The PCR products were digested with NdeI and XhoI, and the DNA fragments were cloned into pET21b (Novagen) using T4 ligase (NEB).

Article Title: Crystal structure of the papain-like protease of MERS coronavirus reveals unusual, potentially druggable active-site features.
Article Snippet: The resulting PCR product was digested with restriction enzymes NheI and XhoI for ligation into pET-28a (Novagen). .. The recombinant plasmid was used to transform Escherichia coli strain Tuner (DE3) pLacI (Novagen).

Article Title: A new subunit vaccine based on nucleoprotein nanoparticles confers partial clinical and virological protection in calves against bovine respiratory syncytial virus.
Article Snippet: .. Plasmid constructions The amplified full-length cDNA coding for BRSV N protein was digested subsequently by NcoI and XhoI and cloned into pET-28a(+) vector (Novagen, Merck Chemicals products, Germany). .. The PCT coding for amino acid residues 161-241 of BRSV P protein was digested subsequently by BamHI and XhoI and inserted into the pGEX-4T3 expression vector (Pharmacia, France).

Article Title: Characterization of trans- and cis-cleavage activity of the SARS coronavirus 3CLpro protease: basis for the in vitro screening of anti-SARS drugs.
Article Snippet: .. Each RT-PCR product was digested with EcoRI and XhoI, and then ligated into the EcoRI/XhoI cleavage sites of the pET24a vector (Novagen) and expressed as a histidine tag fusion protein. .. The Escherichia coli strain BL21(DE3) was transformed with the resulting plasmid, pET24a-3CL pro , then cultured in LB medium in the presence of 100 Â g of kanamycin per ml at 37°C.

Article Title: Binding characterization of determinants in porcine aminopeptidase N, the cellular receptor for transmissible gastroenteritis virus.
Article Snippet: .. The amplicons were inserted into the multiple cloning sites BamHI and XhoI of pET-30a(+) vector (Novagen, Germany) using standard molecular cloning techniques. .. Expression and purification of pAPN in E. coli The above-mentioned recombinant plasmids were transformed into E. coli BL21(DE3)pLysS (Novagen, Germany) and the transformed cells were cultured in Luria bertani (LB) medium containing Ampicillin (50 g/ml) at 37 • C with shaking until the optical density (OD) of the culture at 600 nm reached 0.5.

Positron Emission Tomography:

Article Title: Crystal structure of the papain-like protease of MERS coronavirus reveals unusual, potentially druggable active-site features.
Article Snippet: .. The resulting PCR product was digested with restriction enzymes NheI and XhoI for ligation into pET-28a (Novagen). .. Cloning was designed to include an N-terminal hexahistidine (His 6 ) tag and a thrombin cleavage site.

Article Title: A new subunit vaccine based on nucleoprotein nanoparticles confers partial clinical and virological protection in calves against bovine respiratory syncytial virus.
Article Snippet: .. Plasmid constructions The amplified full-length cDNA coding for BRSV N protein was digested subsequently by NcoI and XhoI and cloned into pET-28a(+) vector (Novagen, Merck Chemicals products, Germany). .. The PCT coding for amino acid residues 161-241 of BRSV P protein was digested subsequently by BamHI and XhoI and inserted into the pGEX-4T3 expression vector (Pharmacia, France).

Article Title: Binding characterization of determinants in porcine aminopeptidase N, the cellular receptor for transmissible gastroenteritis virus.
Article Snippet: .. The amplicons were inserted into the multiple cloning sites BamHI and XhoI of pET-30a(+) vector (Novagen, Germany) using standard molecular cloning techniques. .. Expression and purification of pAPN in E. coli The above-mentioned recombinant plasmids were transformed into E. coli BL21(DE3)pLysS (Novagen, Germany) and the transformed cells were cultured in Luria bertani (LB) medium containing Ampicillin (50 g/ml) at 37 • C with shaking until the optical density (OD) of the culture at 600 nm reached 0.5.

Article Title: Cefoperazone induces esterase B expression by EstR and esterase B enhances cefoperazone activity at the periplasm.
Article Snippet: Paragraph title: 2.4.2. Construction of periplasmic PhoA (pET-phoA) ... The fragment was digested with XhoI before ligating with pETBlue2 Novagen which was digested with EcoRV and XhoI to obtain pETphoA before introducing into E. coli.

Lysis:

Article Title: Oligomerization of the carboxyl terminal domain of the human coronavirus 229E nucleocapsid protein.
Article Snippet: The PCR products were digested with NdeI and XhoI, and the DNA fragments were cloned into pET21b (Novagen) using T4 ligase (NEB). .. The induction of protein expression was initiated by adding IPTG to 1 mM followed by incubation at 37°C for 6 h. After harvesting the bacteria by centrifugation (6000 rpm, 30 min, 4°C), the bacterial cells were lysed with lysis buffer (50 mM Tris-HCl, pH 7.3, 150 mM NaCl, and 15 mM imidazole).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore xho i
    An abnormal <t>T-DNA</t> integration in KLP-7 . (A) Hygromycin phosphotransferase ( hph ) gene insertion in BCM-29. The expected PCR product size is 1.1 kb. Lane 1, Ladder of 10 kb; lane 2, WT, lane 3, BCM-29, lane 4, positive control (PC) ( hph ) ( B) . Southern hybridization showing the integration of 4.1 kb fragment of hph gene in genome of BCM 29. Total genomic DNA was digested with <t>Xho</t> I and probed with the hph probe; ( C) Schematic presentation integration of T-DNA in BCM 29. Specific primers were used for the confirmation of T-DNA insertion shown by arrows. The T-DNA insertion point is 116 bp from KLP-7 start codon ( D) Insertion of T-DNA in B. cinerea genome. Junction site are shown in bold letters.
    Xho I, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 251 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xho i/product/Millipore
    Average 99 stars, based on 251 article reviews
    Price from $9.99 to $1999.99
    xho i - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    87
    Millipore xhoi digested expression vector
    Results of cloning and sequencing of the full-length AS566 antibacterial substance gene. (A) RACE PCR results of the antibacterial substance gene AS566 . M: marker DL2000; 1: 5′RACE DNA fragments of AS566 ; 2: 3′RACE DNA fragments of AS566 . (B) PCR amplification of the full-length AS566 antibacterial substance gene. M: marker DL2000; 1: full-length DNA fragments of AS566 . (C) Restriction analysis of the recombinant pMD18T-AS566 plasmid with <t>EcoRI</t> and <t>XhoI.</t> M: marker DL2000; 1: double digestion product of pMD18T-AS566 plasmid. 4 µg DNA was loaded into each lane.
    Xhoi Digested Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi digested expression vector/product/Millipore
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xhoi digested expression vector - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    Image Search Results


    An abnormal T-DNA integration in KLP-7 . (A) Hygromycin phosphotransferase ( hph ) gene insertion in BCM-29. The expected PCR product size is 1.1 kb. Lane 1, Ladder of 10 kb; lane 2, WT, lane 3, BCM-29, lane 4, positive control (PC) ( hph ) ( B) . Southern hybridization showing the integration of 4.1 kb fragment of hph gene in genome of BCM 29. Total genomic DNA was digested with Xho I and probed with the hph probe; ( C) Schematic presentation integration of T-DNA in BCM 29. Specific primers were used for the confirmation of T-DNA insertion shown by arrows. The T-DNA insertion point is 116 bp from KLP-7 start codon ( D) Insertion of T-DNA in B. cinerea genome. Junction site are shown in bold letters.

    Journal: Scientific Reports

    Article Title: A Botrytis cinerea KLP-7 Kinesin acts as a Virulence Determinant during Plant Infection

    doi: 10.1038/s41598-017-09409-5

    Figure Lengend Snippet: An abnormal T-DNA integration in KLP-7 . (A) Hygromycin phosphotransferase ( hph ) gene insertion in BCM-29. The expected PCR product size is 1.1 kb. Lane 1, Ladder of 10 kb; lane 2, WT, lane 3, BCM-29, lane 4, positive control (PC) ( hph ) ( B) . Southern hybridization showing the integration of 4.1 kb fragment of hph gene in genome of BCM 29. Total genomic DNA was digested with Xho I and probed with the hph probe; ( C) Schematic presentation integration of T-DNA in BCM 29. Specific primers were used for the confirmation of T-DNA insertion shown by arrows. The T-DNA insertion point is 116 bp from KLP-7 start codon ( D) Insertion of T-DNA in B. cinerea genome. Junction site are shown in bold letters.

    Article Snippet: For this purpose, genomic DNA (20 µg) was first digested with Xho I, the fragments were separated on 0.8% agarose gel for 8 h and then transferred to a nylon membrane (Millipore, USA).

    Techniques: Polymerase Chain Reaction, Positive Control, Hybridization

    Results of cloning and sequencing of the full-length AS566 antibacterial substance gene. (A) RACE PCR results of the antibacterial substance gene AS566 . M: marker DL2000; 1: 5′RACE DNA fragments of AS566 ; 2: 3′RACE DNA fragments of AS566 . (B) PCR amplification of the full-length AS566 antibacterial substance gene. M: marker DL2000; 1: full-length DNA fragments of AS566 . (C) Restriction analysis of the recombinant pMD18T-AS566 plasmid with EcoRI and XhoI. M: marker DL2000; 1: double digestion product of pMD18T-AS566 plasmid. 4 µg DNA was loaded into each lane.

    Journal: Journal of Insect Science

    Article Title: Cloning, Expression, and Purification of a New Antibacterial Substance Gene From Larvae ofMusca domestica(Diptera: Muscidae)

    doi: 10.1093/jisesa/ieu115

    Figure Lengend Snippet: Results of cloning and sequencing of the full-length AS566 antibacterial substance gene. (A) RACE PCR results of the antibacterial substance gene AS566 . M: marker DL2000; 1: 5′RACE DNA fragments of AS566 ; 2: 3′RACE DNA fragments of AS566 . (B) PCR amplification of the full-length AS566 antibacterial substance gene. M: marker DL2000; 1: full-length DNA fragments of AS566 . (C) Restriction analysis of the recombinant pMD18T-AS566 plasmid with EcoRI and XhoI. M: marker DL2000; 1: double digestion product of pMD18T-AS566 plasmid. 4 µg DNA was loaded into each lane.

    Article Snippet: Construction of Expression Vector and Recombinant Protein Expression The recombinant plasmid pMD18T-AS566 was digested with EcoRI and XhoI enzymes, and ligated into the EcoRI- or XhoI-digested expression vector, pET-30a(+) (Millipore).

    Techniques: Clone Assay, Sequencing, Polymerase Chain Reaction, Marker, Amplification, Recombinant, Plasmid Preparation

    Location of tranposons in an L . interrogans sph2 mutant and in sph2 complemented isolates. A. Genetic organization of L . interrogans sph1 - sph2 locus. The location of the transposon insertion in the sph2 mutant is designated with

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Role of sph2 Gene Regulation in Hemolytic and Sphingomyelinase Activities Produced by Leptospira interrogans

    doi: 10.1371/journal.pntd.0003952

    Figure Lengend Snippet: Location of tranposons in an L . interrogans sph2 mutant and in sph2 complemented isolates. A. Genetic organization of L . interrogans sph1 - sph2 locus. The location of the transposon insertion in the sph2 mutant is designated with "Tn." The ends of the sequence cloned for complementation studies are marked with dashed lines. The segment of sph2 encoding the enzymatic domain is shaded gray, and the catalytic residues demonstrated experimentally to be necessary for Bacillus subtilis sphingomyelinase activity are abbreviated by their single-letter amino acid codes. B. Genetic structure of the complementing transposon. The sph2 sequences were cloned between the Kpn I and Xho I restriction sites in the transposon. The aadA gene encodes resistance to spectinomycin. C. Insertion sites of the complementing transposon. The location of the complementing transposon in four transconjugants (TC) is indicated by the vertical line. The arrow above the vertical line depicts the orientation of the sph2 gene in the transposon. The dashed open arrow indicates a pseudogene. HP, hypothetical protein.

    Article Snippet: Each amplicon was digested with Nde I and Xho I and ligated to pET20b+ (EMD Biosciences; La Jolla, California USA) that had been digested with the same enzymes.

    Techniques: Mutagenesis, Sequencing, Clone Assay, Activity Assay

    Results of cloning and sequencing of the full-length AS566 antibacterial substance gene. (A) RACE PCR results of the antibacterial substance gene AS566 . M: marker DL2000; 1: 5′RACE DNA fragments of AS566 ; 2: 3′RACE DNA fragments of AS566 . (B) PCR amplification of the full-length AS566 antibacterial substance gene. M: marker DL2000; 1: full-length DNA fragments of AS566 . (C) Restriction analysis of the recombinant pMD18T-AS566 plasmid with EcoRI and XhoI. M: marker DL2000; 1: double digestion product of pMD18T-AS566 plasmid. 4 µg DNA was loaded into each lane.

    Journal: Journal of Insect Science

    Article Title: Cloning, Expression, and Purification of a New Antibacterial Substance Gene From Larvae ofMusca domestica(Diptera: Muscidae)

    doi: 10.1093/jisesa/ieu115

    Figure Lengend Snippet: Results of cloning and sequencing of the full-length AS566 antibacterial substance gene. (A) RACE PCR results of the antibacterial substance gene AS566 . M: marker DL2000; 1: 5′RACE DNA fragments of AS566 ; 2: 3′RACE DNA fragments of AS566 . (B) PCR amplification of the full-length AS566 antibacterial substance gene. M: marker DL2000; 1: full-length DNA fragments of AS566 . (C) Restriction analysis of the recombinant pMD18T-AS566 plasmid with EcoRI and XhoI. M: marker DL2000; 1: double digestion product of pMD18T-AS566 plasmid. 4 µg DNA was loaded into each lane.

    Article Snippet: Construction of Expression Vector and Recombinant Protein Expression The recombinant plasmid pMD18T-AS566 was digested with EcoRI and XhoI enzymes, and ligated into the EcoRI- or XhoI-digested expression vector, pET-30a(+) (Millipore).

    Techniques: Clone Assay, Sequencing, Polymerase Chain Reaction, Marker, Amplification, Recombinant, Plasmid Preparation